##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2869102 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.23497317279065 33.0 33.0 33.0 22.0 33.0 2 29.34261521549251 33.0 33.0 33.0 15.0 33.0 3 29.09548911122714 33.0 33.0 33.0 15.0 33.0 4 32.15837708105184 37.0 37.0 37.0 6.0 37.0 5 32.263680412895745 37.0 37.0 37.0 15.0 37.0 6 33.344937893459345 37.0 37.0 37.0 15.0 37.0 7 32.34438266746878 37.0 37.0 37.0 15.0 37.0 8 32.84421223086527 37.0 33.0 37.0 15.0 37.0 9 31.84158980754257 37.0 33.0 37.0 6.0 37.0 10-11 31.98066015080677 37.0 33.0 37.0 10.5 37.0 12-13 31.95804279527183 37.0 33.0 37.0 10.5 37.0 14-15 33.36953984208299 37.0 33.0 40.0 10.5 40.0 16-17 33.168126647292425 37.0 33.0 40.0 10.5 40.0 18-19 33.344498731658895 37.0 33.0 40.0 10.5 40.0 20-21 33.27581574304433 37.0 33.0 40.0 10.5 40.0 22-23 34.75569620738475 37.0 33.0 40.0 18.5 40.0 24-25 33.7019248531422 37.0 33.0 40.0 15.0 40.0 26-27 33.373726517913965 37.0 33.0 40.0 10.5 40.0 28-29 32.92353199711965 37.0 33.0 40.0 6.0 40.0 30-31 32.884745993694196 37.0 33.0 40.0 10.5 40.0 32-33 32.621777127477515 37.0 33.0 40.0 6.0 40.0 34-35 33.08058392486569 37.0 33.0 40.0 10.5 40.0 36-37 32.722328798348755 37.0 33.0 40.0 6.0 40.0 38-39 32.56651767695955 37.0 33.0 40.0 6.0 40.0 40-41 32.332474760395414 37.0 33.0 40.0 6.0 40.0 42-43 32.24540431117472 37.0 33.0 40.0 6.0 40.0 44-45 32.0645119971336 37.0 33.0 40.0 6.0 40.0 46-47 31.989493228194746 37.0 33.0 40.0 6.0 40.0 48-49 31.885696813846284 37.0 33.0 40.0 6.0 40.0 50-51 31.09248137570571 35.0 30.0 38.5 10.5 38.5 52-53 31.518521823204615 37.0 33.0 38.5 10.5 40.0 54-55 32.05351047122061 37.0 33.0 40.0 6.0 40.0 56-57 32.55050290996974 37.0 33.0 40.0 10.5 40.0 58-59 31.71043587854318 37.0 33.0 40.0 6.0 40.0 60-61 31.560740085225273 37.0 33.0 40.0 6.0 40.0 62-63 31.06945587852924 37.0 33.0 37.0 6.0 40.0 64-65 30.86425229915144 37.0 33.0 37.0 6.0 40.0 66-67 30.754133697582034 37.0 30.0 37.0 6.0 40.0 68-69 31.10539604377955 37.0 27.0 37.0 10.5 38.5 70-71 30.633114298480848 37.0 27.0 37.0 6.0 37.0 72-73 31.31268355046283 37.0 27.0 37.0 15.0 37.0 74-75 31.679061427582567 37.0 27.0 37.0 18.5 37.0 76-77 31.383309830044382 33.0 27.0 37.0 22.0 37.0 78-79 30.829877780573852 33.0 27.0 37.0 15.0 37.0 80-81 30.113908986156645 33.0 27.0 37.0 15.0 37.0 82-83 29.60280237509855 33.0 27.0 37.0 6.0 37.0 84-85 29.259845240775682 33.0 27.0 37.0 6.0 37.0 86-87 29.042437842920886 33.0 27.0 37.0 6.0 37.0 88-89 28.901859536537913 33.0 27.0 37.0 6.0 37.0 90-91 28.7965086288323 33.0 27.0 37.0 6.0 37.0 92-93 28.550314000687322 33.0 24.5 37.0 6.0 37.0 94-95 28.365919371287603 33.0 22.0 37.0 6.0 37.0 96-97 28.2242250362657 33.0 22.0 37.0 6.0 37.0 98-99 28.08812844576456 33.0 22.0 37.0 6.0 37.0 100-101 26.850803491824273 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.2672074644019844 2306 2 -0.2529446799151387 2306 3 -0.20994431445227235 2306 4 0.00868833840139871 2306 5 -0.0035909004419352186 2306 6 -0.5910923078551065 2306 7 0.30082766210491485 2306 8 -0.6802546315935771 2306 9 -0.0573132612344196 2306 10-11 -0.16693128090249942 2306 12-13 -0.20312065223876985 2306 14-15 0.3155840320055958 2306 16-17 0.18041922097099672 2306 18-19 0.15144621307710793 2306 20-21 0.17165217195294957 2306 22-23 -0.16551295110224373 2306 24-25 0.22561037009565865 2306 26-27 -0.3155082627093151 2306 28-29 -0.041886456601808675 2306 30-31 0.09702092036585697 2306 32-33 0.19759512330118412 2306 34-35 -0.1604335508296657 2306 36-37 0.3829144710693342 2306 38-39 0.09689252438182194 2306 40-41 0.0710722001967028 2306 42-43 0.11950553504542683 2306 44-45 0.1366629774571848 2306 46-47 0.24450950940446603 2306 48-49 0.08833310237646685 2306 50-51 -0.07827275422682689 2306 52-53 -0.036894235725672075 2306 54-55 0.13066780055782345 2306 56-57 -0.039441731984062756 2306 58-59 0.4619324464796293 2306 60-61 0.31594278961756217 2306 62-63 0.41409932360416946 2306 64-65 0.358711819981572 2306 66-67 0.1089776566006222 2306 68-69 -0.16029447741553682 2306 70-71 0.17602043070263207 2306 72-73 -0.5138759476889554 2306 74-75 -0.5555865101749653 2306 76-77 -0.2839040842849698 2306 78-79 -0.011219595576655195 2306 80-81 0.4104925385285263 2306 82-83 0.48496739971477254 2306 84-85 0.431293742238946 2306 86-87 0.41524392576788216 2306 88-89 0.3475899483254743 2306 90-91 0.3028323248921403 2306 92-93 0.35777958490686856 2306 94-95 0.27987100531796827 2306 96-97 0.07056579383943884 2306 98-99 0.16348387038329903 2306 100-101 0.051536983674253634 2307 1 -0.2519084044413695 2307 2 -0.2994108559683646 2307 3 -0.37133210382210535 2307 4 -0.09729732740119346 2307 5 -0.12852419804254822 2307 6 -0.6269056660432355 2307 7 0.23837168568851297 2307 8 -0.5929642154733799 2307 9 -0.040132897155924496 2307 10-11 -0.16842343828142603 2307 12-13 -0.1620655724607225 2307 14-15 0.37144635271942406 2307 16-17 0.24932833336859517 2307 18-19 0.25316697687195955 2307 20-21 0.15435559747195526 2307 22-23 -0.15535175462522943 2307 24-25 0.405740150236646 2307 26-27 -0.3453923210927883 2307 28-29 -0.010571508228487403 2307 30-31 0.17697565364717605 2307 32-33 0.2544985943557023 2307 34-35 -0.048610387487642015 2307 36-37 0.39876473989482975 2307 38-39 -0.14949852994693202 2307 40-41 0.09239572205176927 2307 42-43 0.1586190315002156 2307 44-45 0.20181244617010208 2307 46-47 0.15556151395485784 2307 48-49 0.05790830987527684 2307 50-51 -0.16254822189464235 2307 52-53 -0.09676462950502085 2307 54-55 0.012839346085030456 2307 56-57 -0.02324016385511385 2307 58-59 0.2545113055148249 2307 60-61 0.14338546112870532 2307 62-63 0.2162735451328217 2307 64-65 0.18425895752962873 2307 66-67 0.16753386488444733 2307 68-69 -0.08463211764868461 2307 70-71 0.06963277177305116 2307 72-73 -0.5195226269569382 2307 74-75 -0.5467938492966837 2307 76-77 -0.05590204151163647 2307 78-79 0.1421843612689706 2307 80-81 0.3433742126223791 2307 82-83 0.38107292824679817 2307 84-85 0.5394310792182786 2307 86-87 0.4014796139034331 2307 88-89 0.3307617729157535 2307 90-91 0.3949497219056113 2307 92-93 0.40569503116666894 2307 94-95 0.3940954550379949 2307 96-97 0.29897961275833396 2307 98-99 0.23301148695537321 2307 100-101 0.11981988406454747 2201 1 -0.4933716298147459 2201 2 -0.6193468403687845 2201 3 -0.6184277886167457 2201 4 -0.3290293796799837 2201 5 -0.47543476406935525 2201 6 -0.7238409219979616 2201 7 0.013761470964105627 2201 8 -0.7184516095016065 2201 9 -0.22529640388309247 2201 10-11 -0.4031510471157418 2201 12-13 -0.44195848500342905 2201 14-15 -0.09268864957877554 2201 16-17 -0.27718270878397533 2201 18-19 -0.22635653350858576 2201 20-21 -0.33217047769085895 2201 22-23 -0.336588727439576 2201 24-25 -0.13549926929387368 2201 26-27 -0.7116836451635038 2201 28-29 -0.48547503187104013 2201 30-31 -0.2000876260811708 2201 32-33 -0.07993241975802334 2201 34-35 -0.5271797156914175 2201 36-37 0.006742055605862163 2201 38-39 -0.4160176696533142 2201 40-41 -0.22615706946944414 2201 42-43 -0.2188846757017444 2201 44-45 -0.1294166741333349 2201 46-47 -0.17742987315870806 2201 48-49 -0.15498170744851336 2201 50-51 -0.14009969389700316 2201 52-53 -0.2832524816892068 2201 54-55 -0.2024946028257517 2201 56-57 -0.10923072747604579 2201 58-59 0.19106700859115477 2201 60-61 -0.06159542552559216 2201 62-63 0.0013451236471624384 2201 64-65 -0.04338505529280212 2201 66-67 -0.3215217212395878 2201 68-69 -0.5931996154562924 2201 70-71 -0.1100527268112721 2201 72-73 -0.6400975607025394 2201 74-75 -0.4817364461424596 2201 76-77 -0.09865525064535063 2201 78-79 0.15258364752585152 2201 80-81 0.3099562095437527 2201 82-83 0.29466105792349495 2201 84-85 0.13503707029426337 2201 86-87 0.15272617680557232 2201 88-89 0.1362020778679245 2201 90-91 0.032974679895605874 2201 92-93 0.058561977330747084 2201 94-95 0.016543388992253938 2201 96-97 0.0466217246419518 2201 98-99 -0.07985705198750637 2201 100-101 -0.18098539742662112 1101 1 -0.3923049084159693 1101 2 -0.5227774896099326 1101 3 -0.4679517024802422 1101 4 -0.24254964350354058 1101 5 -0.2870555371234076 1101 6 -0.5723476229831377 1101 7 0.2771620545267268 1101 8 -0.5436816785084417 1101 9 -0.17880970600433344 1101 10-11 -0.28716242991506746 1101 12-13 -0.32172662703325017 1101 14-15 0.08348124924078348 1101 16-17 -0.09942042456901135 1101 18-19 -0.04955495034536028 1101 20-21 -0.07442939754530897 1101 22-23 -0.1315374715595823 1101 24-25 0.16941913178893486 1101 26-27 -0.42086997193637643 1101 28-29 -0.1045326226227914 1101 30-31 0.05173566039704269 1101 32-33 -0.06359322121360123 1101 34-35 -0.29736379863274465 1101 36-37 0.1148814227843431 1101 38-39 -0.18249312063849032 1101 40-41 0.03745881375146354 1101 42-43 -0.08124648175038374 1101 44-45 0.11706676478119249 1101 46-47 0.09270428522417973 1101 48-49 0.022717477671747588 1101 50-51 0.18176324100137364 1101 52-53 -0.05774983846119497 1101 54-55 0.16736802143092433 1101 56-57 0.2587808744366171 1101 58-59 0.3744229235418999 1101 60-61 0.2708889591819599 1101 62-63 0.264791471388353 1101 64-65 0.17257714349569753 1101 66-67 0.12583768565665565 1101 68-69 -0.05429347639975646 1101 70-71 0.14043195744363146 1101 72-73 -0.19977439626213922 1101 74-75 0.20397112353003521 1101 76-77 0.4529542616471858 1101 78-79 0.5087811573998167 1101 80-81 0.6468197407632417 1101 82-83 0.5831577462611186 1101 84-85 0.40289619428467915 1101 86-87 0.5020632202732216 1101 88-89 0.42984376931196167 1101 90-91 0.44350051030742677 1101 92-93 0.5627497463686453 1101 94-95 0.5003026527714312 1101 96-97 0.41381823879833846 1101 98-99 0.46188438595071446 1101 100-101 0.5155332660374761 2304 1 -0.19148859345079572 2304 2 -0.38981204325127905 2304 3 -0.3913178489790283 2304 4 -0.2932553863012153 2304 5 -0.3756098688218792 2304 6 -0.3682250907323876 2304 7 0.12768355331088088 2304 8 -0.348826407622731 2304 9 -0.22139319217669495 2304 10-11 -0.32085957165351786 2304 12-13 -0.26781657752371046 2304 14-15 -0.057939071824215205 2304 16-17 -0.18609126210971993 2304 18-19 -0.19966230258952322 2304 20-21 -0.21016977383843027 2304 22-23 -0.18554717769649187 2304 24-25 0.08239555164633572 2304 26-27 -0.36749732763230014 2304 28-29 -0.1510959340434752 2304 30-31 -0.10693942486652475 2304 32-33 -0.1000569210858373 2304 34-35 -0.3593251054579554 2304 36-37 0.09285585916897077 2304 38-39 -0.25372603572525065 2304 40-41 -0.2018441179243382 2304 42-43 0.03684468973501254 2304 44-45 0.1295528225131264 2304 46-47 0.04035831347707841 2304 48-49 -0.03242934439271039 2304 50-51 -0.02170854474234929 2304 52-53 -0.3056364417769437 2304 54-55 -0.15598537417142921 2304 56-57 0.16390792617176686 2304 58-59 0.2180294759570991 2304 60-61 0.047557330261380315 2304 62-63 0.08411513293062 2304 64-65 0.15413551555397476 2304 66-67 0.03108210359755148 2304 68-69 0.1017415937172963 2304 70-71 0.14189541260169136 2304 72-73 -0.1558010301800401 2304 74-75 -0.013212383765026203 2304 76-77 0.22572877943146707 2304 78-79 0.23845265438081853 2304 80-81 0.34942295218598574 2304 82-83 0.17170724807457916 2304 84-85 0.29730460420925553 2304 86-87 0.27539623376083355 2304 88-89 0.21892108095932983 2304 90-91 0.21678168302926792 2304 92-93 0.183174396298579 2304 94-95 0.30615093513552694 2304 96-97 0.1354822694210931 2304 98-99 0.1581797675502692 2304 100-101 0.1467089180075547 2202 1 -0.014857130992883327 2202 2 -0.06702265640295835 2202 3 0.032436413017940424 2202 4 0.1655168617237024 2202 5 0.24046606017917327 2202 6 -0.014194516562803017 2202 7 0.3313442250306977 2202 8 0.05633210037109393 2202 9 0.19458483631241208 2202 10-11 0.1712577747854631 2202 12-13 0.1477776263385202 2202 14-15 0.39581540390340564 2202 16-17 0.4060214534985036 2202 18-19 0.4176734498581425 2202 20-21 0.3366114128772608 2202 22-23 0.23350931428296917 2202 24-25 0.35138077039074744 2202 26-27 0.316221680681231 2202 28-29 0.47575958937992624 2202 30-31 0.595783652047416 2202 32-33 0.362081881535687 2202 34-35 0.15086346434559061 2202 36-37 0.4219534171181749 2202 38-39 0.2954293423019365 2202 40-41 0.35942088255358584 2202 42-43 0.3436488049999511 2202 44-45 0.4976246098891295 2202 46-47 0.4445124358366854 2202 48-49 0.39912326629750794 2202 50-51 0.3192823817978301 2202 52-53 0.20481528389026593 2202 54-55 0.5035299737672219 2202 56-57 0.622991419456774 2202 58-59 0.60784197480681 2202 60-61 0.4471304923410493 2202 62-63 0.5703658698560901 2202 64-65 0.5375003246835988 2202 66-67 0.46806109803046425 2202 68-69 0.40310344386479713 2202 70-71 0.5415491689475616 2202 72-73 0.19341481581040298 2202 74-75 0.4272884168343687 2202 76-77 0.7803049010869536 2202 78-79 0.6772200103486234 2202 80-81 0.815282597642458 2202 82-83 0.8029436561410144 2202 84-85 0.7246648288164188 2202 86-87 0.6001568405896478 2202 88-89 0.5695438386427973 2202 90-91 0.5054164666240162 2202 92-93 0.6995470546863523 2202 94-95 0.660577207857024 2202 96-97 0.47614428823770183 2202 98-99 0.6816165207917031 2202 100-101 0.7158826946815857 1102 1 -0.009746951022485462 1102 2 -0.2482239181813597 1102 3 -0.18869888293136938 1102 4 -0.18658943047095988 1102 5 -0.20013902709834497 1102 6 -0.0308571781209821 1102 7 -0.19366344672641134 1102 8 -0.1598901684833791 1102 9 -0.2741457022449332 1102 10-11 -0.14709546562935216 1102 12-13 -0.13563640146934475 1102 14-15 -0.20706934599731142 1102 16-17 -0.13055294336600554 1102 18-19 -0.0294558745308251 1102 20-21 -0.1065311362671153 1102 22-23 0.043646582325138183 1102 24-25 -0.15861775730881078 1102 26-27 -0.0509706141669497 1102 28-29 0.026968003943629526 1102 30-31 0.01976119297849266 1102 32-33 -0.039450833995559265 1102 34-35 -0.08995073472296866 1102 36-37 -0.12027678523569563 1102 38-39 -0.09691785434776534 1102 40-41 -0.026901305296156863 1102 42-43 0.0536513096029978 1102 44-45 0.049064728440342265 1102 46-47 0.1490169418657814 1102 48-49 -0.05849140390719754 1102 50-51 0.17828775541240205 1102 52-53 -0.00865248570429955 1102 54-55 0.20865287418781975 1102 56-57 0.43976066766970234 1102 58-59 0.1789342017373876 1102 60-61 0.03151121795388789 1102 62-63 0.18580494256629976 1102 64-65 0.18059523560096125 1102 66-67 0.1274432620977599 1102 68-69 0.2575310066829495 1102 70-71 0.1912523678446334 1102 72-73 0.21358321651981882 1102 74-75 0.4456103779159939 1102 76-77 0.3918738261835273 1102 78-79 0.2643027739411714 1102 80-81 0.2430270559261487 1102 82-83 0.14174170616086812 1102 84-85 0.13604743175335088 1102 86-87 0.18722338631332036 1102 88-89 0.1769458996377793 1102 90-91 0.22790292258813238 1102 92-93 0.11811276328593934 1102 94-95 0.08786530941303283 1102 96-97 -0.08128671107634844 1102 98-99 0.08337953006099141 1102 100-101 0.10053091641341538 2305 1 0.12004066428541904 2305 2 0.0739011753115335 2305 3 0.09125351360316003 2305 4 0.08954611864397322 2305 5 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1213 80-81 -0.35816453997481545 1213 82-83 -0.0904439799081409 1213 84-85 -0.17374804752752837 1213 86-87 -0.08466359458889627 1213 88-89 -0.07058205953309837 1213 90-91 -0.1323998975147056 1213 92-93 -0.02766318488490427 1213 94-95 0.04819452669061519 1213 96-97 -0.004636929643837107 1213 98-99 0.003829558501639241 1213 100-101 0.2399134576635511 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 378.0 3 277.0 4 320.0 5 251.0 6 704.0 7 5568.0 8 33940.0 9 108244.0 10 126043.0 11 76492.0 12 41879.0 13 25428.0 14 17452.0 15 13435.0 16 11015.0 17 10003.0 18 9382.0 19 9371.0 20 9596.0 21 10345.0 22 10967.0 23 11416.0 24 12364.0 25 14167.0 26 16865.0 27 21520.0 28 27768.0 29 35172.0 30 41434.0 31 53102.0 32 74846.0 33 109036.0 34 169814.0 35 295134.0 36 542893.0 37 771679.0 38 150739.0 39 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.599367412671725 18.760796629740124 21.761954091315825 11.877881866272325 2 34.289122580582074 25.81650662347631 29.797855986970255 10.096514808971358 3 39.28397865048975 17.75508950092308 30.06705237668312 12.893879471904054 4 29.546117768944107 14.4523379675202 35.373443693721995 20.628100569813697 5 38.56230050909781 12.584068516473677 31.722522664680035 17.131108309748473 6 36.676471542769164 12.53784136620113 36.76720357930905 14.018483511720659 7 42.32944355353088 16.57699377384008 25.580117321453244 15.513445351175797 8 29.124957520346623 35.99012308806669 19.901202967958305 14.983716423628387 9 38.26636287234254 27.376375979316308 18.457214644226077 15.900046504115073 10-11 32.57885706053542 30.08129967863134 22.72859190435611 14.611251356477132 12-13 36.39608668277822 21.475670690018823 22.377832700263202 19.750409926939756 14-15 31.234032472270908 21.601713227955717 24.67719535679776 22.48705894297561 16-17 33.625283433362476 23.186022119271303 26.115779835749503 17.072914611616707 18-19 33.09459343255383 23.318007861985986 26.64099378405301 16.946404921407176 20-21 30.1401129052627 27.68514558304035 25.33515043220347 16.83959107949348 22-23 35.72317180644399 23.165859128144426 23.979839122490116 17.131129942921465 24-25 29.6830463271206 28.824407063383035 24.544052368632286 16.948494240864076 26-27 24.006084013437626 32.77034211052834 25.255796381087464 17.967777494946574 28-29 26.47504431298907 30.797239599921994 23.710632431212392 19.017083655876544 30-31 30.37637121019829 25.62155069868482 25.036616230827324 18.96546186028957 32-33 25.745258257068414 27.745803428503173 26.95038049368923 19.558557820739182 34-35 33.30751266266411 23.643829808485826 24.12452734377592 18.92413018507414 36-37 27.257240620927263 25.054000470457655 27.526330522869845 20.162428385745237 38-39 20.17839386495825 29.946241197223067 29.382353547253224 20.493011390565457 40-41 23.112234001590153 31.836250157382594 25.182367352013653 19.869148489013604 42-43 28.874169155031737 25.64199294856983 27.19322375270428 18.290614143694153 44-45 23.820275614922817 29.310082591577256 27.45423865730057 19.415403136199355 46-47 26.862073777525193 30.584500994564955 24.297233189983476 18.25619203792638 48-49 21.39360165695395 28.384365116648652 29.288966272394983 20.933066954002413 50-51 18.502393363095596 25.39107722383503 31.134013488896255 24.972515924173116 52-53 27.891090412574098 25.405705955429887 24.881889928782954 21.821313703213065 54-55 31.558347365326046 24.96514990777646 25.085274474158204 18.391228252739296 56-57 33.101884106710756 24.675508358887914 23.160009205585233 19.0625983288161 58-59 29.46197485238483 24.085459916612294 23.298935627266477 23.153629603736402 60-61 22.29155224971798 23.782356356612883 24.510353650059233 29.415737743609903 62-63 26.8760978177787 23.448506269616278 25.278762585794084 24.39663332681094 64-65 23.663416440191185 27.713354916945796 28.081586683538358 20.54164195932466 66-67 20.89889178323232 27.242048794777823 26.428907245788302 25.43015217620156 68-69 19.11969873864614 23.22318366161306 36.66794217212062 20.989175427620186 70-71 18.47990965059427 31.5561707631697 30.860134721157923 19.103784865078104 72-73 20.275987367395004 37.989651346525925 24.105481413829533 17.628879872249534 74-75 21.335771817633116 37.40239982880807 23.57481146720631 17.687016886352506 76-77 20.494632905841765 36.45177924425672 23.666570540996666 19.387017308904845 78-79 20.403370380520638 36.9127886983677 24.02483622024428 18.65900470086738 80-81 19.5845948044063 37.413036833296935 23.950135735247574 19.052232627049186 82-83 19.46197713319877 36.47815291562192 24.176858014904628 19.88301193627468 84-85 20.316310794614843 34.69094237415036 24.41475274272448 20.57799408851031 86-87 19.37190583686287 34.58797690622799 25.18006213470715 20.86005512220198 88-89 19.089692111091047 34.636306073503995 25.27530556813828 20.998696247266675 90-91 20.183034534992515 33.20079272946055 24.650439725306775 21.96573301024015 92-93 20.84921258907861 32.91755751095197 23.876960148363104 22.356269751606312 94-95 21.142901731210138 34.29932370840035 23.296414443055312 21.261360117334203 96-97 20.592038544201515 34.79000704076223 22.582184270347113 22.03577014468915 98-99 20.810074130878007 32.723779412404056 24.323507940823106 22.142638515894827 100-101 21.916341406854624 33.720758048608765 23.545411112570164 20.81748943196645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 688.0 1 514.5 2 350.5 3 509.5 4 546.0 5 396.0 6 424.5 7 559.0 8 662.5 9 737.5 10 796.0 11 819.5 12 889.5 13 914.5 14 816.0 15 771.5 16 732.5 17 678.5 18 658.0 19 625.0 20 622.0 21 661.0 22 716.5 23 831.0 24 969.5 25 1172.0 26 1453.0 27 1759.5 28 2146.5 29 2741.0 30 3466.0 31 4626.0 32 7023.5 33 11232.0 34 17644.5 35 27776.0 36 41862.5 37 60942.5 38 88957.0 39 113197.5 40 125291.5 41 127562.0 42 149281.5 43 188233.5 44 189398.5 45 173067.0 46 179248.5 47 182273.5 48 166103.5 49 144027.0 50 122736.5 51 111539.5 52 106553.0 53 105803.5 54 95749.5 55 67143.5 56 45758.5 57 37883.0 58 33933.5 59 28688.0 60 22257.0 61 16970.5 62 12280.5 63 8742.0 64 6537.0 65 4800.0 66 3474.0 67 2627.0 68 2076.0 69 1610.5 70 1153.0 71 878.0 72 676.0 73 468.5 74 354.0 75 283.0 76 218.5 77 166.0 78 123.5 79 105.0 80 84.0 81 70.0 82 69.0 83 53.0 84 36.5 85 31.5 86 32.0 87 27.0 88 17.0 89 15.0 90 12.5 91 8.5 92 10.5 93 7.5 94 2.5 95 2.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20703342021301438 2 0.19169761130834664 3 0.16639352661564488 4 0.15008180259886195 5 0.16113055583245212 6 0.12272132534848883 7 0.10909336789002273 8 0.10539883210844368 9 0.09372270487420803 10-11 0.08019930974918285 12-13 0.10604363316466267 14-15 0.1022445350496427 16-17 0.09367042370748757 18-19 0.08795434947938414 20-21 0.09159660409424272 22-23 0.07885742647002442 24-25 0.3906448777352635 26-27 0.18134594029769593 28-29 0.0892613786473956 30-31 0.1720050385103074 32-33 0.2015438977073663 34-35 0.20086424254000032 36-37 0.20677201437941212 38-39 0.2071379825464553 40-41 0.20412310193224223 42-43 0.21883153683626444 44-45 0.20199700115227692 46-47 0.21931949439232207 48-49 0.20971718677133122 50-51 0.21193042282916397 52-53 0.20504673587763694 54-55 0.18849103308282522 56-57 0.1959846669794242 58-59 0.19136649725245042 60-61 0.17228387139948317 62-63 0.16076458766540888 64-65 0.1651213515587804 66-67 0.17728543634907368 68-69 0.17001835417493 70-71 0.16266413672291888 72-73 0.16618440194876308 74-75 0.15618127204958207 76-77 0.16716031706087828 78-79 0.16510392450320693 80-81 0.17031461411967924 82-83 0.19385856619945893 84-85 0.1926038181981679 86-87 0.1967863115358046 88-89 0.20316461387570048 90-91 0.18561556891320002 92-93 0.16653294306023278 94-95 0.16747400406120103 96-97 0.17655350001498726 98-99 0.18997233280657153 100-101 0.2004111390950897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2869102.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.51852247760428 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83642819982636 34.7796524541193 2 5.766463288045793 4.6729714470592665 3 2.460665331893848 2.9910757068060745 4 1.2907612044731278 2.09198947506656 5 0.7328673883440949 1.4847351873861678 6 0.5076144375049748 1.2340672197601066 7 0.43954767200696615 1.2466875559735024 8 0.3264782763622106 1.0582733903385386 9 0.2470828189867194 0.9010287679448897 >10 1.9541064874801668 15.053630511273841 >50 0.22797654828801903 6.386698322443199 >100 0.18633417771491337 15.121955815907398 >500 0.016284253358690586 4.39593041652065 >1k 0.006615101327765226 4.572840265004603 >5k 4.3045243704185063E-4 1.3974315776874207 >10k+ 3.44361949458369E-4 2.6110318867084663 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 21770 0.758773999669583 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 21548 0.7510363869949552 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19975 0.6962108701607681 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11559 0.4028786707478508 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9812 0.3419885385740904 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 9703 0.33818944045907046 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7703 0.2684812181651262 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6913 0.24094647035901828 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5930 0.20668487910154468 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4643 0.16182763805539155 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4359 0.15192907048965146 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4006 0.13962556925477032 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3534 0.12317442879339947 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3265 0.11379867289486396 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2970 0.10351671010650719 No Hit TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA 2933 0.10222710799406923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007423925674305061 0.0 0.0 2 0.007877029119215699 0.0 0.0 3 0.008330132564126336 0.0 0.0 4 0.008783236009036974 0.0 0.0 5 0.010630503899826496 0.0 0.0 6 0.011327586122765939 0.0 0.0 7 0.013209708124702433 0.0 0.0 8 0.014778143126316179 0.0 0.0 9 0.016869389795134505 0.0 0.0 10-11 0.024972970636805522 0.0 0.0 12-13 0.03243175042225756 0.0 0.0 14-15 0.04046562304163463 0.0 0.0 16-17 0.04790697577151318 0.0 0.0 18-19 0.05520891205680384 0.0 0.0 20-21 0.06082042395146635 0.0 0.0 22-23 0.06678047695759859 0.0 0.0 24-25 0.07329819574208238 0.0 0.0 26-27 0.07857859358084865 0.0 0.0 28-29 0.08659503914465222 0.0 0.0 30-31 0.1690075849516678 0.0 0.0 32-33 0.18092769096393227 0.0 0.0 34-35 0.18997233280657155 0.0 0.0 36-37 0.20008002503919345 0.0 0.0 38-39 0.21217440160719278 0.0 0.0 40-41 0.22721395056711124 1.742705557348606E-5 0.0 42-43 0.2432642687502919 3.485411114697212E-5 0.0 44-45 0.2601162314898529 6.970822229394424E-5 0.0 46-47 0.2783797857308663 6.970822229394424E-5 0.0 48-49 0.2988740030852859 6.970822229394424E-5 0.0 50-51 0.32126776949721547 6.970822229394424E-5 0.0 52-53 0.345020846243877 6.970822229394424E-5 0.0 54-55 0.3718933659381925 6.970822229394424E-5 0.0 56-57 0.4027741084144098 6.970822229394424E-5 0.0 58-59 0.4359726492819007 6.970822229394424E-5 0.0 60-61 0.4710533121513282 6.970822229394424E-5 0.0 62-63 0.5098110837467612 6.970822229394424E-5 0.0 64-65 0.5513746112895255 6.970822229394424E-5 0.0 66-67 0.5954824889460186 6.970822229394424E-5 0.0 68-69 0.6437031517178546 6.970822229394424E-5 0.0 70-71 0.6971345041061628 6.970822229394424E-5 0.0 72-73 0.7543823816650645 6.970822229394424E-5 0.0 74-75 0.8176600204523924 6.970822229394424E-5 0.0 76-77 0.8893026459149935 6.970822229394424E-5 0.0 78-79 0.9747300723362222 6.970822229394424E-5 0.0 80-81 1.0649847931513066 6.970822229394424E-5 0.0 82-83 1.1712026968717042 6.970822229394424E-5 0.0 84-85 1.2936277622754435 6.970822229394424E-5 0.0 86-87 1.4276941008022719 6.970822229394424E-5 0.0 88-89 1.5760157707882119 6.970822229394424E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4565 0.0 90.43869 9 ATTCGGA 1030 0.0 90.37554 2 AAGCGGC 1510 0.0 88.62331 9 CGGGAGA 6265 0.0 88.59552 4 GTTCGGA 1000 0.0 87.3876 2 ATCGGGA 7830 0.0 84.91773 2 AGAGCGG 5095 0.0 84.2908 8 ATCCGGA 1180 0.0 82.91198 2 TCGGGAG 6805 0.0 81.98667 3 TCGGAGA 1605 0.0 81.945114 4 CGGAAAG 3000 0.0 80.084335 5 ACCGGAA 625 0.0 79.77058 3 CGGAGAG 2665 0.0 79.46135 5 GGATCGG 2325 0.0 78.86143 1 GTCCGGA 540 0.0 77.39644 2 TCGGGAA 2130 0.0 77.3541 3 TTGGGAG 3060 0.0 76.189224 3 AAGGGGC 4420 0.0 76.01281 9 GGAAAGC 1855 0.0 75.72887 6 GGAGAGC 6355 0.0 75.57508 6 >>END_MODULE