##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3425843 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27598637765946 33.0 33.0 33.0 33.0 33.0 2 32.30027091142239 33.0 33.0 33.0 33.0 33.0 3 32.2992492650714 33.0 33.0 33.0 33.0 33.0 4 36.123165305590476 37.0 37.0 37.0 33.0 37.0 5 36.07373776323083 37.0 37.0 37.0 33.0 37.0 6 36.060552979222926 37.0 37.0 37.0 33.0 37.0 7 36.019802717170634 37.0 37.0 37.0 33.0 37.0 8 35.941610283950546 37.0 37.0 37.0 33.0 37.0 9 35.94562768930158 37.0 37.0 37.0 33.0 37.0 10-11 35.85080912931504 37.0 37.0 37.0 33.0 37.0 12-13 35.875372134683346 37.0 37.0 37.0 33.0 37.0 14-15 37.696911679840554 40.0 37.0 40.0 33.0 40.0 16-17 37.50183750393698 40.0 37.0 40.0 33.0 40.0 18-19 37.206325713116456 40.0 37.0 40.0 33.0 40.0 20-21 37.09721417473013 40.0 37.0 40.0 33.0 40.0 22-23 36.98635532918467 40.0 37.0 40.0 33.0 40.0 24-25 36.81076745198189 40.0 37.0 40.0 33.0 40.0 26-27 36.69229033554661 38.5 37.0 40.0 33.0 40.0 28-29 36.621768860978165 37.0 37.0 40.0 33.0 40.0 30-31 36.585769692306386 37.0 37.0 40.0 33.0 40.0 32-33 36.49738297989721 37.0 37.0 40.0 33.0 40.0 34-35 36.41927694876853 37.0 37.0 40.0 33.0 40.0 36-37 36.32577543687787 37.0 37.0 40.0 33.0 40.0 38-39 36.2507839676249 37.0 37.0 40.0 33.0 40.0 40-41 36.1063006973758 37.0 37.0 40.0 33.0 40.0 42-43 36.0035020577417 37.0 37.0 40.0 33.0 40.0 44-45 35.86683700916825 37.0 37.0 40.0 27.0 40.0 46-47 35.727240273415916 37.0 37.0 40.0 27.0 40.0 48-49 35.57746312367496 37.0 37.0 40.0 27.0 40.0 50-51 34.287596804640486 37.0 35.0 38.5 24.5 38.5 52-53 34.73510169613727 37.0 35.0 38.5 27.0 40.0 54-55 35.4813899819694 37.0 37.0 40.0 27.0 40.0 56-57 35.457260008704424 37.0 37.0 40.0 27.0 40.0 58-59 35.26141930613866 37.0 37.0 40.0 27.0 40.0 60-61 35.0222526251203 37.0 35.0 40.0 27.0 40.0 62-63 34.76827469910326 37.0 33.0 40.0 27.0 40.0 64-65 34.49318386744518 37.0 33.0 38.5 27.0 40.0 66-67 34.18680745147982 37.0 33.0 37.0 27.0 40.0 68-69 33.922903209516605 37.0 33.0 37.0 27.0 40.0 70-71 33.61258601167654 37.0 33.0 37.0 22.0 40.0 72-73 33.3368271692544 37.0 33.0 37.0 22.0 38.5 74-75 33.017216492407854 37.0 33.0 37.0 22.0 37.0 76-77 32.74111569035709 37.0 33.0 37.0 22.0 37.0 78-79 32.4834453884781 37.0 33.0 37.0 22.0 37.0 80-81 32.24831143750603 37.0 33.0 37.0 22.0 37.0 82-83 31.99588495444771 37.0 33.0 37.0 18.5 37.0 84-85 31.785119166289874 37.0 33.0 37.0 15.0 37.0 86-87 31.58732463805259 33.0 33.0 37.0 15.0 37.0 88-89 31.34986074960236 33.0 33.0 37.0 15.0 37.0 90-91 31.16189767015009 33.0 33.0 37.0 15.0 37.0 92-93 30.922741351544715 33.0 33.0 37.0 15.0 37.0 94-95 30.733042203043162 33.0 30.0 37.0 15.0 37.0 96-97 30.547704900662406 33.0 27.0 37.0 15.0 37.0 98-99 30.329691699240158 33.0 27.0 37.0 15.0 37.0 100-101 29.063442195103512 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21682422267453916 2306 2 0.2504912310380263 2306 3 0.24548887600318636 2306 4 0.3038623256764055 2306 5 0.40392106686355334 2306 6 0.4021827622305807 2306 7 0.35018444068762733 2306 8 0.35521590381348034 2306 9 0.2490979451822426 2306 10-11 0.33892985998246417 2306 12-13 0.25858395850650595 2306 14-15 0.3754180367739508 2306 16-17 0.37390935699654193 2306 18-19 0.37086679098774056 2306 20-21 0.3442100251528757 2306 22-23 0.41537521080670814 2306 24-25 0.44492967571311226 2306 26-27 0.4850562332427657 2306 28-29 0.39842238929238505 2306 30-31 0.31384211904855874 2306 32-33 0.28714325110403394 2306 34-35 0.33639146858029534 2306 36-37 0.34733585355383667 2306 38-39 0.31178587599762864 2306 40-41 0.22050170134968283 2306 42-43 0.22866229680209926 2306 44-45 0.3798810638026353 2306 46-47 0.41221500053601545 2306 48-49 0.373456295598686 2306 50-51 0.5389926750392462 2306 52-53 0.4867394054711838 2306 54-55 0.43318693107010375 2306 56-57 0.31387605441617694 2306 58-59 0.4356395659960768 2306 60-61 0.4806747115977714 2306 62-63 0.5144598823889552 2306 64-65 0.5261555481539872 2306 66-67 0.5561915039019567 2306 68-69 0.36472386306431304 2306 70-71 0.5399547650461685 2306 72-73 0.43376419380511066 2306 74-75 0.4590445574240718 2306 76-77 0.3442608662436726 2306 78-79 0.327995109725137 2306 80-81 0.33989352028971354 2306 82-83 0.41309644143783686 2306 84-85 0.5104643370940423 2306 86-87 0.5139447825652894 2306 88-89 0.6169041463122262 2306 90-91 0.6518318210970193 2306 92-93 0.6062809433277856 2306 94-95 0.5414863559844001 2306 96-97 0.6471572322754078 2306 98-99 0.7094269410890561 2306 100-101 0.5480225765288758 2307 1 0.2532428532578095 2307 2 0.22838532451562799 2307 3 0.19591817517740395 2307 4 0.24190862319244388 2307 5 0.35157298284521943 2307 6 0.25175074586414325 2307 7 0.2916399545757429 2307 8 0.2940565501200041 2307 9 0.30883300862180363 2307 10-11 0.25904647224222543 2307 12-13 0.1931094284001844 2307 14-15 0.3226241956512794 2307 16-17 0.36143065170080035 2307 18-19 0.46631599273514013 2307 20-21 0.4739027279095893 2307 22-23 0.4037160485205078 2307 24-25 0.48863321903496626 2307 26-27 0.44638267706069 2307 28-29 0.3099348747906632 2307 30-31 0.4346925107885653 2307 32-33 0.5266780196422545 2307 34-35 0.4306094211037248 2307 36-37 0.5430849610937685 2307 38-39 0.35614980021480136 2307 40-41 0.46099496804416873 2307 42-43 0.27083784943256006 2307 44-45 0.3435508279537913 2307 46-47 0.33265346249515915 2307 48-49 0.2674508880422053 2307 50-51 0.1616388212867932 2307 52-53 0.32162715619666216 2307 54-55 0.3292424622244283 2307 56-57 0.291654581237097 2307 58-59 0.3312622381104191 2307 60-61 0.5180331811375254 2307 62-63 0.4931687895858303 2307 64-65 0.5145153030288512 2307 66-67 0.5771894666956854 2307 68-69 0.5197751922012444 2307 70-71 0.3618097956180151 2307 72-73 0.6126326586568567 2307 74-75 0.41509732667287835 2307 76-77 0.39222670391448844 2307 78-79 0.4744587356358707 2307 80-81 0.4823582303257439 2307 82-83 0.7088790667476914 2307 84-85 0.730279169042582 2307 86-87 0.580810583880762 2307 88-89 0.5927556163304253 2307 90-91 0.714196799859927 2307 92-93 0.9252418202310686 2307 94-95 0.7607974755260649 2307 96-97 0.5970210803080036 2307 98-99 0.5612716910437392 2307 100-101 0.3738221791309151 2201 1 0.25010649183597167 2201 2 0.14738368995863027 2201 3 0.19268584606609807 2201 4 0.24962331370399937 2201 5 0.36183335119766014 2201 6 0.30308217902632606 2201 7 0.3458190286493945 2201 8 0.32976715734534423 2201 9 0.2255315827740887 2201 10-11 0.1847795787408586 2201 12-13 0.19758328840377004 2201 14-15 0.2633383979864874 2201 16-17 0.20622776423412148 2201 18-19 0.36160469853154353 2201 20-21 0.42253900578885606 2201 22-23 0.4035219877924163 2201 24-25 0.5121085998076182 2201 26-27 0.495780042477854 2201 28-29 0.3433315489788882 2201 30-31 0.36509190313488915 2201 32-33 0.2633109630121524 2201 34-35 0.18798969609775185 2201 36-37 0.23877337967376633 2201 38-39 0.23415614748834912 2201 40-41 0.1872808124666463 2201 42-43 0.28610366031376344 2201 44-45 0.4142486062445627 2201 46-47 0.2854833812643065 2201 48-49 0.2543544864545453 2201 50-51 0.13746346493930872 2201 52-53 0.2820525006912362 2201 54-55 0.3784218976074385 2201 56-57 0.3698048146494841 2201 58-59 0.4085427132469519 2201 60-61 0.46104243359990704 2201 62-63 0.39185748184541325 2201 64-65 0.30751724755985066 2201 66-67 0.3398544224017712 2201 68-69 0.3621857011046643 2201 70-71 0.4505308618501047 2201 72-73 0.36329830166816635 2201 74-75 0.35486648432506485 2201 76-77 0.4479780459089966 2201 78-79 0.42941939869249524 2201 80-81 0.5660342898368285 2201 82-83 0.7077414327321705 2201 84-85 0.5687048934841954 2201 86-87 0.7204767601912323 2201 88-89 0.7605446580955011 2201 90-91 0.703595966919238 2201 92-93 0.7354801830451763 2201 94-95 0.7288065563256296 2201 96-97 0.7042921940988727 2201 98-99 0.8017590368782699 2201 100-101 0.6563351985294688 1101 1 0.17751865805177403 1101 2 0.22863503616934366 1101 3 0.08554405372267837 1101 4 0.22094917762142074 1101 5 0.3136793179199344 1101 6 0.21000304069853826 1101 7 0.28508180411301254 1101 8 0.29712344897139076 1101 9 0.19424488522215455 1101 10-11 0.17355431098943797 1101 12-13 0.20175965995669998 1101 14-15 0.3804847415869119 1101 16-17 0.4164449559381751 1101 18-19 0.5043904237694861 1101 20-21 0.5257089441278424 1101 22-23 0.6138215493384322 1101 24-25 0.6363287102790309 1101 26-27 0.5901119933972936 1101 28-29 0.5331280931875355 1101 30-31 0.5595524209803244 1101 32-33 0.488559928331334 1101 34-35 0.42029067595661473 1101 36-37 0.5639847271500784 1101 38-39 0.28813965344087933 1101 40-41 0.38296227497779256 1101 42-43 0.31721778430023306 1101 44-45 0.4111110225969625 1101 46-47 0.5006266393802079 1101 48-49 0.39882670188232794 1101 50-51 0.5110127517670691 1101 52-53 0.5442658262606628 1101 54-55 0.4538360133188917 1101 56-57 0.6068316786265342 1101 58-59 0.6033268540633259 1101 60-61 0.5829277431401465 1101 62-63 0.5649890022208055 1101 64-65 0.5516317208005361 1101 66-67 0.6408266755538818 1101 68-69 0.5368411776419606 1101 70-71 0.7031827448414276 1101 72-73 0.6713920094945038 1101 74-75 0.7241640270113621 1101 76-77 0.5881063359871348 1101 78-79 0.8104729355911431 1101 80-81 0.8054628089381168 1101 82-83 0.6845602927031322 1101 84-85 0.6275782626678037 1101 86-87 0.8156824551036763 1101 88-89 0.9410504469536782 1101 90-91 1.0687386622125814 1101 92-93 1.1448735850611165 1101 94-95 1.0650833755810964 1101 96-97 0.9278051560545002 1101 98-99 1.0435677630009046 1101 100-101 0.9165550674435323 2304 1 0.15083536544864273 2304 2 0.1541037673900476 2304 3 0.11787949701421496 2304 4 0.15906597315578352 2304 5 0.23113221109996118 2304 6 0.18503575840482256 2304 7 0.20769274384565506 2304 8 0.216364131102182 2304 9 0.1306106157617961 2304 10-11 0.1237666422261654 2304 12-13 0.1675154055865562 2304 14-15 0.2198942203738241 2304 16-17 0.1977613094496533 2304 18-19 0.3143698249686864 2304 20-21 0.4215047188697696 2304 22-23 0.3309352467063249 2304 24-25 0.4677941296666148 2304 26-27 0.46165994152971734 2304 28-29 0.4230146495487972 2304 30-31 0.32955696139670465 2304 32-33 0.3317528092255273 2304 34-35 0.31962947782085394 2304 36-37 0.377679090356672 2304 38-39 0.18732738630414758 2304 40-41 0.27664725688290304 2304 42-43 0.36277802494229405 2304 44-45 0.37748272912580916 2304 46-47 0.31086978535459764 2304 48-49 0.36413508295680685 2304 50-51 0.3177646180117435 2304 52-53 0.38784263942253006 2304 54-55 0.4632022913793321 2304 56-57 0.4531227259937509 2304 58-59 0.4637850021076062 2304 60-61 0.5980413203205472 2304 62-63 0.5443985477785347 2304 64-65 0.4386145807286894 2304 66-67 0.5004026140178865 2304 68-69 0.5612367347109881 2304 70-71 0.5994150338789908 2304 72-73 0.6591186964595082 2304 74-75 0.7329944691677852 2304 76-77 0.6334395558100567 2304 78-79 0.7014812418822913 2304 80-81 0.6791424157914676 2304 82-83 0.76880671712523 2304 84-85 0.7797957219042075 2304 86-87 0.9425169836348459 2304 88-89 0.8734007467547009 2304 90-91 0.8433520591117372 2304 92-93 0.9923631688117673 2304 94-95 1.0326870039079559 2304 96-97 1.0316488188351052 2304 98-99 0.9941030981236914 2304 100-101 1.0469927168432633 2202 1 0.23842197288415434 2202 2 0.2501876083722223 2202 3 0.22639626616729203 2202 4 0.2564796703347554 2202 5 0.34579086414004223 2202 6 0.2866128558265757 2202 7 0.3424544421746063 2202 8 0.31101238193534186 2202 9 0.30191598196127245 2202 10-11 0.29920538777841443 2202 12-13 0.24797837680124246 2202 14-15 0.423720392245869 2202 16-17 0.43284451451763317 2202 18-19 0.489663249273498 2202 20-21 0.5346928517944747 2202 22-23 0.5450653810337585 2202 24-25 0.44554046837676253 2202 26-27 0.487389291814722 2202 28-29 0.391876692922871 2202 30-31 0.5335755942365665 2202 32-33 0.4243765695578787 2202 34-35 0.39599330734348115 2202 36-37 0.32318865751711456 2202 38-39 0.3290337283776523 2202 40-41 0.38364422634642636 2202 42-43 0.4184079299277528 2202 44-45 0.3577337667031557 2202 46-47 0.25525895024266276 2202 48-49 0.30709621012101707 2202 50-51 0.40683464762228283 2202 52-53 0.3875359480241798 2202 54-55 0.47679413919091473 2202 56-57 0.5231868888804314 2202 58-59 0.4568946728873229 2202 60-61 0.4829854726539651 2202 62-63 0.49508630937567233 2202 64-65 0.5014824782392537 2202 66-67 0.5829852992272677 2202 68-69 0.47933439713469284 2202 70-71 0.5635441612164058 2202 72-73 0.5884138991086942 2202 74-75 0.5444727833859346 2202 76-77 0.49681417069930944 2202 78-79 0.5056554169183016 2202 80-81 0.5865445854521738 2202 82-83 0.6886744791956332 2202 84-85 0.7057616896694583 2202 86-87 0.707416345052124 2202 88-89 0.8536517843320475 2202 90-91 0.7645169502032516 2202 92-93 0.9125155314369273 2202 94-95 0.9783881509199368 2202 96-97 0.9443957139072481 2202 98-99 1.0458529446835918 2202 100-101 0.7728783268048112 1102 1 0.1490023128497029 1102 2 0.0427654847017962 1102 3 0.16761991177858704 1102 4 0.15060329878811274 1102 5 0.17846929859727112 1102 6 0.13881317892989387 1102 7 0.19490766295952966 1102 8 0.10492461845107215 1102 9 0.10822938597956977 1102 10-11 0.1145067348609885 1102 12-13 0.14886406716839673 1102 14-15 0.042918843715568755 1102 16-17 0.11885123627807559 1102 18-19 0.31686670527950156 1102 20-21 0.29798139684108094 1102 22-23 0.26045360282204655 1102 24-25 0.461615150576435 1102 26-27 0.31258470922765014 1102 28-29 0.3455069312345245 1102 30-31 0.4117028183440965 1102 32-33 0.5117489030284545 1102 34-35 0.5010064762658999 1102 36-37 0.391846204843624 1102 38-39 0.39590382207618546 1102 40-41 0.3587692175379331 1102 42-43 0.45219832723227427 1102 44-45 0.5222991529158278 1102 46-47 0.3946293005947865 1102 48-49 0.3346513858729807 1102 50-51 0.3998805575537503 1102 52-53 0.417359452628034 1102 54-55 0.486333902373282 1102 56-57 0.5976243584680248 1102 58-59 0.6516542656803281 1102 60-61 0.6357547499615848 1102 62-63 0.5689152029298228 1102 64-65 0.6732453133927763 1102 66-67 0.6862373058648501 1102 68-69 0.6173126775043869 1102 70-71 0.6691654888493446 1102 72-73 0.6533843389348419 1102 74-75 0.6861816324558561 1102 76-77 0.7080351251740353 1102 78-79 0.6963557694130742 1102 80-81 0.7699993180191385 1102 82-83 0.7997115850445837 1102 84-85 0.9057495038405676 1102 86-87 0.8165201131083535 1102 88-89 0.8360386114271741 1102 90-91 0.7054358308573683 1102 92-93 0.8819950379609374 1102 94-95 1.002069079656568 1102 96-97 0.8284657950622467 1102 98-99 0.9274264213566106 1102 100-101 1.0009895316744668 2305 1 0.17058589019368497 2305 2 0.1334897642134365 2305 3 0.13655158890625785 2305 4 0.15611083605399045 2305 5 0.1412652471807334 2305 6 0.20637424606833576 2305 7 0.17522307198119336 2305 8 0.15227064846533267 2305 9 0.18727919059224973 2305 10-11 0.09894981679752135 2305 12-13 0.06033683165755832 2305 14-15 0.10386705169077004 2305 16-17 0.14081669150110798 2305 18-19 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1213 92-93 -0.9254644935394865 1213 94-95 -1.0500269896890657 1213 96-97 -1.1287761029632186 1213 98-99 -1.330605630282509 1213 100-101 -0.9298489264920455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 479.0 3 196.0 4 95.0 5 157.0 6 350.0 7 894.0 8 2307.0 9 4099.0 10 4153.0 11 5515.0 12 5992.0 13 8047.0 14 11515.0 15 15298.0 16 17098.0 17 16776.0 18 16552.0 19 17662.0 20 19285.0 21 21226.0 22 23815.0 23 25822.0 24 28167.0 25 31455.0 26 36211.0 27 41091.0 28 47742.0 29 56593.0 30 67475.0 31 83627.0 32 108585.0 33 145405.0 34 211575.0 35 345387.0 36 677001.0 37 1003625.0 38 324393.0 39 178.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.71705167813509 32.69238870945092 19.364292234236764 15.226267378177221 2 30.138856753569993 26.484342161340134 30.44011135217069 12.936689732919179 3 33.736019990554496 24.3845286677009 24.81834943407372 17.061101907670892 4 29.25733178836779 24.319348237397232 29.247419916313394 17.175900057921588 5 29.0179164095831 22.848378692134265 30.407775961541272 17.725928936741358 6 26.629003490586058 23.44395332400718 31.95303872732449 17.974004458082277 7 30.651469767770816 23.4174727318266 26.031201786967333 19.89985571343525 8 27.068100727132133 28.67070801061079 25.127074968459574 19.1341162937975 9 24.98555039129365 26.581465111427082 29.05171995635605 19.381264540923222 10-11 20.056756038582773 31.713437224405915 32.99512273792813 15.234683999083185 12-13 20.26920832272735 31.015184649298284 32.9709910921439 15.744615935830472 14-15 20.317529154668048 30.994189814963637 33.11006212298235 15.578218907385965 16-17 19.98780034348973 31.07701051435067 33.06833049526953 15.866858646890073 18-19 19.311766276828067 31.45528835842945 32.7054191130193 16.527526251723184 20-21 19.32035888818939 31.714583634970893 32.19605927782598 16.768998199013733 22-23 19.385132022981146 31.206802429121765 32.24528981860472 17.162775729292374 24-25 19.273412406929854 30.909195896208136 32.46379929618129 17.35359240068072 26-27 19.30606880493316 31.339715052817585 32.243711546513296 17.110504595735957 28-29 19.09334173981435 31.593648510147965 32.04147476478019 17.271534985257496 30-31 18.564746079401036 32.60486546824595 31.856250918333558 16.974137534019462 32-33 18.309417119056533 33.00498993822538 31.951194543242234 16.734398399475854 34-35 18.68744295546311 32.49831562774254 32.362278894607215 16.451962522187134 36-37 18.692791759921995 32.04408803273637 32.83114032489783 16.43197988244381 38-39 18.96273335347752 31.92427385133532 32.19151095142667 16.921481843760485 40-41 18.882246119552008 31.90988693967264 31.606260430260157 17.601606510515193 42-43 19.14133692808794 31.506043622674806 31.579237600758738 17.77338184847852 44-45 18.823404328753117 31.448685632187605 31.81302732511511 17.91488271394417 46-47 18.963623510227627 31.66252007048604 31.69448072421525 17.67937569507108 48-49 18.905269987322452 32.20920763273838 31.180582816217665 17.7049395637215 50-51 19.443177646368806 32.11288267824678 30.327189809580503 18.11674986580391 52-53 19.560185439091356 31.94292039805142 30.401606760491863 18.095287402365358 54-55 19.913610069661754 31.994923056226565 30.301750609759665 17.789716264352016 56-57 20.042081083298346 32.06918986456577 30.186508871378503 17.70222018075738 58-59 19.884531026839472 32.61254181626594 30.056838097332665 17.446089059561924 60-61 19.688869492879643 32.49742868859829 29.87404046407145 17.939661354450617 62-63 19.47080353058829 32.37067463976132 30.30451828350807 17.854003546142316 64-65 19.38580866693254 31.98115474894245 30.693734116295907 17.939302467829105 66-67 19.842176451417693 31.616993957339513 30.35937958958333 18.181450001659467 68-69 20.196034199260207 31.639885842622643 30.48011039716048 17.68396956095667 70-71 19.925800723865425 31.936724489942108 30.50099568774086 17.636479098451606 72-73 20.021446474817935 31.892443078029643 30.175833316886653 17.91027713026577 74-75 20.418994499155016 31.4643041484449 29.818406773680085 18.298294578719997 76-77 20.527438019307816 31.269685909913914 29.406358114889326 18.79651795588894 78-79 20.36009258003097 31.4336016626045 29.632027708515587 18.57427804884895 80-81 20.18664669000591 31.792408225635683 29.27400978540587 18.74693529895254 82-83 19.882289750533648 31.900085942198935 29.133396419031172 19.084227888236242 84-85 20.00507423444133 31.856014576359915 29.691246726241395 18.44766446295736 86-87 20.346006218354894 31.547921709154082 29.95924478326938 18.14682728922165 88-89 20.832672170176597 31.455488595063514 29.767610351848 17.94422888291189 90-91 20.897146082333435 31.441946360735894 29.26183868448988 18.39906887244079 92-93 20.641284440150915 31.55299964679767 29.21246406575645 18.59325184729496 94-95 20.599951083237293 31.333920353978417 29.24892009594469 18.817208466839595 96-97 20.728051078786425 31.764803066832563 28.96279477106218 18.544351083318837 98-99 21.316972608937373 31.51127400216475 28.646967663519753 18.524785725378123 100-101 21.773692899709165 31.376095858628005 28.08995681863285 18.760254423029984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 154325.0 1 86055.5 2 13231.0 3 8687.0 4 7810.5 5 6710.5 6 6996.5 7 7817.0 8 8181.5 9 7999.0 10 7424.0 11 6795.0 12 6343.5 13 6169.0 14 6025.5 15 5928.5 16 6121.0 17 6164.5 18 6150.5 19 6135.0 20 6147.0 21 6501.5 22 7032.5 23 7682.5 24 8863.0 25 10602.5 26 12776.5 27 15797.0 28 19342.5 29 23095.0 30 27746.5 31 33652.0 32 42559.5 33 57663.0 34 77768.0 35 99096.0 36 127766.0 37 165679.5 38 194540.0 39 199253.5 40 198186.0 41 197434.5 42 190128.0 43 184964.5 44 173536.5 45 159757.0 46 155651.5 47 144420.5 48 120225.0 49 96722.0 50 76970.5 51 62082.0 52 50500.5 53 39309.5 54 31195.5 55 26353.5 56 21191.0 57 15520.0 58 11091.5 59 7975.0 60 5882.5 61 4489.0 62 3453.0 63 2708.0 64 2141.0 65 1568.5 66 1116.5 67 797.5 68 602.5 69 499.5 70 388.0 71 282.0 72 234.0 73 191.5 74 155.0 75 131.0 76 94.0 77 81.0 78 65.0 79 49.0 80 47.0 81 38.5 82 28.5 83 22.5 84 16.5 85 16.0 86 13.0 87 7.5 88 9.0 89 8.0 90 4.0 91 4.0 92 5.0 93 4.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2266011606486345 2 0.20841585560108852 3 0.1817946706839747 4 0.16644078552344635 5 0.17727023684389506 6 0.13547030614070757 7 0.1220137642034384 8 0.11804393838246527 9 0.10522957415153 10-11 0.09117463935154063 12-13 0.11769365963355588 14-15 0.11445358120614402 16-17 0.10553606805682571 18-19 0.09927483542007035 20-21 0.10366791472930896 22-23 0.09120382924728308 24-25 0.4135916327747652 26-27 0.20019889994958906 28-29 0.09010920815694123 30-31 0.1729647272218838 32-33 0.203249244054675 34-35 0.20180434421542376 36-37 0.20834288086173242 38-39 0.20888289393296774 40-41 0.20446062472798668 42-43 0.21977072504490136 44-45 0.2024903067653713 46-47 0.22118643498841012 48-49 0.21019643924137793 50-51 0.21419545495809353 52-53 0.20545308118322994 54-55 0.18865429618345034 56-57 0.19643340339881307 58-59 0.1917630200800212 60-61 0.1720890303496103 62-63 0.16073416090579748 64-65 0.16553589875543043 66-67 0.17731402168750873 68-69 0.17010411743912374 70-71 0.16328827678326183 72-73 0.16639700067983268 74-75 0.15670595529333947 76-77 0.16712674807339392 78-79 0.16541913917246062 80-81 0.17081926988481375 82-83 0.19511985809040286 84-85 0.1928868310661055 86-87 0.19765937901999597 88-89 0.20416872577056216 90-91 0.1848596097369319 92-93 0.1665867350021586 94-95 0.1677689257797278 96-97 0.17595669153548485 98-99 0.19020136065780013 100-101 0.20116216650908988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3425843.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.70199491794351 #Duplication Level Percentage of deduplicated Percentage of total 1 82.73207001368169 36.982885733025455 2 8.168023357722266 7.302538772530892 3 3.0523492524944262 4.0933830231838355 4 1.4696715372557474 2.6278899835781058 5 0.8526565981414537 1.905772545843513 6 0.6386965692845401 1.7130606476559278 7 0.43366835599336284 1.3570088451981754 8 0.3556300164193052 1.271789694931516 9 0.33004745738991853 1.3278401786631886 >10 1.6942436048125735 14.1308634688891 >50 0.14352626354661974 4.520027447671495 >100 0.1181118302794453 11.015313496142948 >500 0.00936422545305303 2.826410791544332 >1k 0.0018170823597909416 1.0344925014081119 >5k 0.0 0.0 >10k+ 1.2383516612677023E-4 7.8907228697334935 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 159210 4.647323301155365 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125876 3.6743073164765576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.167595829697975E-4 0.0 0.0 2 1.7513937445469627E-4 0.0 0.0 3 2.0432927019714564E-4 0.0 0.0 4 2.9189895742449376E-4 0.0 0.0 5 3.2108885316694315E-4 0.0 0.0 6 4.3784843613674067E-4 0.0 0.0 7 5.254181233640887E-4 0.0 0.0 8 5.837979148489875E-4 0.0 0.0 9 8.465069765310319E-4 0.0 0.0 10-11 0.0023205967115247257 0.0 0.0 12-13 0.008786158618477263 0.0 0.0 14-15 0.020097243218676397 0.0 0.0 16-17 0.042865361897786905 0.0 0.0 18-19 0.06420317568551741 0.0 0.0 20-21 0.07233256164978956 0.0 0.0 22-23 0.07936732652371986 0.0 0.0 24-25 0.0876718518624467 0.0 0.0 26-27 0.09750884672765214 0.0 0.0 28-29 0.10871776669275271 0.0 0.0 30-31 0.12043750983334613 0.0 0.0 32-33 0.13405459619719876 0.0 0.0 34-35 0.14913117734817388 0.0 0.0 36-37 0.165638063390529 0.0 0.0 38-39 0.18429040676995415 0.0 0.0 40-41 0.203833041969524 0.0 0.0 42-43 0.22388650034458674 0.0 0.0 44-45 0.24621677058756047 0.0 0.0 46-47 0.2699189659304294 0.0 0.0 48-49 0.2955768842880424 0.0 0.0 50-51 0.32356999430505134 0.0 0.0 52-53 0.3524679910900762 0.0 0.0 54-55 0.3837741542738532 0.0 0.0 56-57 0.4149635578746603 0.0 0.0 58-59 0.4472621775136806 0.0 0.0 60-61 0.48383711687896963 0.0 0.0 62-63 0.521725601552669 0.0 0.0 64-65 0.5640363554313493 0.0 0.0 66-67 0.6093098837278883 0.0 0.0 68-69 0.658275933835847 0.0 0.0 70-71 0.7119561521062114 0.0 0.0 72-73 0.7705694627570499 0.0 0.0 74-75 0.8364948422913718 0.0 0.0 76-77 0.908871188784775 0.0 2.9189895742449375E-5 78-79 0.9914639987880355 0.0 2.9189895742449375E-5 80-81 1.090563694833651 0.0 2.9189895742449375E-5 82-83 1.203835085262226 0.0 2.9189895742449375E-5 84-85 1.3333798425672163 0.0 2.9189895742449375E-5 86-87 1.478614168833773 0.0 2.9189895742449375E-5 88-89 1.6455949674284547 0.0 5.837979148489875E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGG 155 0.0 55.164177 1 ATGACCG 50 2.8273016E-5 47.502487 1 GGTACGG 70 4.3111504E-6 40.716415 1 CGGCACC 70 4.3111504E-6 40.716415 1 CGCGTAG 50 0.001615847 38.00199 1 ACACGGC 105 4.660069E-8 36.19237 1 GTTCGCG 55 0.0025796727 34.54726 1 CGAGGCG 115 1.0369331E-7 33.045208 1 ACCGGGC 160 5.2750693E-11 32.65796 1 CGTGGCT 190 0.0 32.5017 1 CGCGGAG 105 1.90235E-6 31.668325 1 AACGGTG 60 0.0039510215 31.668325 1 CGGTCGC 60 0.0039510215 31.668325 1 GTGCGAC 60 0.0039510215 31.668325 1 GAGCGGC 210 0.0 31.633623 9 TTGACGT 365 0.0 31.22127 5 CGGCAGG 200 0.0 30.876617 1 ACGGCTG 125 2.1550477E-7 30.40159 1 CGACGCA 110 2.7344176E-6 30.228855 1 GTGTTTG 50565 0.0 29.817638 1 >>END_MODULE