FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425843
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1592104.647323301155365No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1258763.6743073164765576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG1550.055.1641771
ATGACCG502.8273016E-547.5024871
GGTACGG704.3111504E-640.7164151
CGGCACC704.3111504E-640.7164151
CGCGTAG500.00161584738.001991
ACACGGC1054.660069E-836.192371
GTTCGCG550.002579672734.547261
CGAGGCG1151.0369331E-733.0452081
ACCGGGC1605.2750693E-1132.657961
CGTGGCT1900.032.50171
CGCGGAG1051.90235E-631.6683251
AACGGTG600.003951021531.6683251
CGGTCGC600.003951021531.6683251
GTGCGAC600.003951021531.6683251
GAGCGGC2100.031.6336239
TTGACGT3650.031.221275
CGGCAGG2000.030.8766171
ACGGCTG1252.1550477E-730.401591
CGACGCA1102.7344176E-630.2288551
GTGTTTG505650.029.8176381
Produced by FastQC (version 0.11.2)