FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6052628
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC207860.3434210726315908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118840.19634446392542215No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109930.18162358565568543No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG94040.15537052665387663No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA78750.13010877258605683No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT64300.10623484542582164Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG3100.053.6253851
CGGAAAG3050.051.3674055
CGCGGAG4100.050.9721451
CGACGGG3650.048.1474151
GGACGGG9600.046.01251
CGGGAGA8900.045.3421824
CGGAGCG1208.731149E-1139.5806431
CGAGGGG11700.038.5657541
ACCGGTA500.001616900937.9974141
CGACGCG905.70637E-736.941931
GTCCCGT2450.036.7940149
GTTACGG2250.035.8864481
GATCCGG3200.035.6225781
TCGGCCG801.0805228E-535.6146162
CGTTTGA6200.035.2395361
GGTCCGG2450.034.8955841
GCACGGG3300.034.5431061
GAGCGGC5950.034.2879189
GAACCGG2100.033.9262661
CCAGCCG3650.033.8257522
Produced by FastQC (version 0.11.2)