##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3920376 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.298135178870595 33.0 33.0 33.0 33.0 33.0 2 32.3027676426955 33.0 33.0 33.0 33.0 33.0 3 32.28912456356227 33.0 33.0 33.0 33.0 33.0 4 36.10029777756011 37.0 37.0 37.0 37.0 37.0 5 36.042661979361164 37.0 37.0 37.0 37.0 37.0 6 36.068057502647704 37.0 37.0 37.0 33.0 37.0 7 36.028942376955676 37.0 37.0 37.0 33.0 37.0 8 35.97891299201913 37.0 37.0 37.0 33.0 37.0 9 35.971574410209634 37.0 37.0 37.0 33.0 37.0 10-11 35.91335754529668 37.0 37.0 37.0 33.0 37.0 12-13 35.93364309953943 37.0 37.0 37.0 33.0 37.0 14-15 37.806326612549405 40.0 37.0 40.0 33.0 40.0 16-17 37.77205260413797 40.0 37.0 40.0 33.0 40.0 18-19 37.71443313090377 40.0 37.0 40.0 33.0 40.0 20-21 37.665636535883294 40.0 37.0 40.0 33.0 40.0 22-23 37.67110119029399 40.0 37.0 40.0 33.0 40.0 24-25 37.514668235903905 40.0 37.0 40.0 33.0 40.0 26-27 37.438579743371555 40.0 37.0 40.0 33.0 40.0 28-29 37.40220236528333 40.0 37.0 40.0 33.0 40.0 30-31 37.38655654457634 40.0 37.0 40.0 33.0 40.0 32-33 37.33649030093032 40.0 37.0 40.0 33.0 40.0 34-35 37.28535528224843 40.0 37.0 40.0 33.0 40.0 36-37 37.21056730272811 40.0 37.0 40.0 33.0 40.0 38-39 37.17533369248255 40.0 37.0 40.0 33.0 40.0 40-41 37.05564734096934 40.0 37.0 40.0 33.0 40.0 42-43 36.98080617777478 40.0 37.0 40.0 33.0 40.0 44-45 36.87252421706489 40.0 37.0 40.0 33.0 40.0 46-47 36.76661626333801 40.0 37.0 40.0 33.0 40.0 48-49 36.64044354419066 38.5 37.0 40.0 33.0 40.0 50-51 35.4493801104792 37.0 35.0 38.5 30.0 38.5 52-53 35.85697379537065 37.0 37.0 38.5 33.0 40.0 54-55 36.64766453523846 37.0 37.0 40.0 33.0 40.0 56-57 36.672613927847735 37.0 37.0 40.0 33.0 40.0 58-59 36.49404189802203 37.0 37.0 40.0 33.0 40.0 60-61 36.25780575638663 37.0 37.0 40.0 33.0 40.0 62-63 36.015706274092075 37.0 37.0 40.0 33.0 40.0 64-65 35.75412894069344 37.0 37.0 40.0 33.0 40.0 66-67 35.48829640320214 37.0 37.0 38.5 33.0 40.0 68-69 35.25374045754795 37.0 33.0 37.0 30.0 40.0 70-71 34.93178218109692 37.0 33.0 37.0 27.0 40.0 72-73 34.68817098155891 37.0 33.0 37.0 27.0 40.0 74-75 34.41512612565734 37.0 33.0 37.0 27.0 37.0 76-77 34.17404975951286 37.0 33.0 37.0 27.0 37.0 78-79 33.94563646446157 37.0 33.0 37.0 27.0 37.0 80-81 33.748210120661895 37.0 33.0 37.0 27.0 37.0 82-83 33.54547063853059 37.0 33.0 37.0 27.0 37.0 84-85 33.38623348882862 37.0 33.0 37.0 27.0 37.0 86-87 33.23520358761506 37.0 33.0 37.0 27.0 37.0 88-89 33.063759828138934 37.0 33.0 37.0 27.0 37.0 90-91 32.93354170620369 37.0 33.0 37.0 27.0 37.0 92-93 32.76979465745123 33.0 33.0 37.0 27.0 37.0 94-95 32.64005952490271 33.0 33.0 37.0 27.0 37.0 96-97 32.50354647105277 33.0 33.0 37.0 27.0 37.0 98-99 32.34954682918169 33.0 33.0 37.0 22.0 37.0 100-101 31.00454803314784 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.24188844999444825 2306 2 0.1872521234562754 2306 3 0.27846615414787124 2306 4 0.34138458910758374 2306 5 0.4774274976149684 2306 6 0.36305811159500934 2306 7 0.29175569438761784 2306 8 0.2886471922356577 2306 9 0.3447558598659981 2306 10-11 0.28261361099988136 2306 12-13 0.2704450951406159 2306 14-15 0.4903084105806812 2306 16-17 0.41357076368682044 2306 18-19 0.4700567507509703 2306 20-21 0.4723681222106322 2306 22-23 0.43501445516539405 2306 24-25 0.5019499708617801 2306 26-27 0.6028204316339725 2306 28-29 0.5327405215323964 2306 30-31 0.4814078684152818 2306 32-33 0.5058943123394712 2306 34-35 0.4881418823472998 2306 36-37 0.43106739934621885 2306 38-39 0.42503155744513066 2306 40-41 0.46701395790989153 2306 42-43 0.4039694982578794 2306 44-45 0.4344494057481185 2306 46-47 0.4743926730163679 2306 48-49 0.5245243891415399 2306 50-51 0.5895646272997865 2306 52-53 0.5823159454657585 2306 54-55 0.43702401916516465 2306 56-57 0.4168008532137719 2306 58-59 0.4860660260989178 2306 60-61 0.5352922740964843 2306 62-63 0.5120917942965733 2306 64-65 0.4834737413518795 2306 66-67 0.49876698653799423 2306 68-69 0.4672495086019097 2306 70-71 0.6561929898610117 2306 72-73 0.4411164309992799 2306 74-75 0.325512286512172 2306 76-77 0.36866058035788996 2306 78-79 0.26433561620761026 2306 80-81 0.41653597653200336 2306 82-83 0.5179249340790477 2306 84-85 0.4980926363649729 2306 86-87 0.511006225145394 2306 88-89 0.3347073685841764 2306 90-91 0.21959702223375643 2306 92-93 0.3662762982585477 2306 94-95 0.2039165607294322 2306 96-97 0.18763520547589252 2306 98-99 0.181386573386078 2306 100-101 0.11691927458544527 2307 1 0.20278052119537193 2307 2 0.1852959410743864 2307 3 0.19949416620259797 2307 4 0.23958993134464635 2307 5 0.32011498541932326 2307 6 0.26248410296591373 2307 7 0.31312952089500357 2307 8 0.3122644764539473 2307 9 0.2886152142011511 2307 10-11 0.19771182664067055 2307 12-13 0.23598533536783606 2307 14-15 0.31653637710498117 2307 16-17 0.3634149303715546 2307 18-19 0.3374759342386042 2307 20-21 0.32207922079485485 2307 22-23 0.3308367672439658 2307 24-25 0.3746154522918701 2307 26-27 0.3478456901817637 2307 28-29 0.34557115917522196 2307 30-31 0.3683354858729544 2307 32-33 0.3603213335653308 2307 34-35 0.2979970522191664 2307 36-37 0.1903011330582558 2307 38-39 0.19845723443925323 2307 40-41 0.3163637942171533 2307 42-43 0.1733448641274009 2307 44-45 0.29283621926848724 2307 46-47 0.30070302793690473 2307 48-49 0.3040904407860552 2307 50-51 0.06911848329354342 2307 52-53 0.24331723549459383 2307 54-55 0.24366685462685922 2307 56-57 0.15118039270599581 2307 58-59 0.22367280579668858 2307 60-61 0.14356309342739593 2307 62-63 0.23523859740998176 2307 64-65 0.2880275973456321 2307 66-67 0.3208584593389858 2307 68-69 0.19756356015970056 2307 70-71 0.17374713855038948 2307 72-73 0.23727263289421074 2307 74-75 0.21467062400105874 2307 76-77 0.25018091728306757 2307 78-79 0.20392838987802264 2307 80-81 0.05911343246505396 2307 82-83 0.14977888980612164 2307 84-85 0.24850349428835727 2307 86-87 0.18852557557792693 2307 88-89 0.2537407142034951 2307 90-91 0.15753823377680476 2307 92-93 0.29072288672850277 2307 94-95 0.24484543569420936 2307 96-97 0.17363812159150172 2307 98-99 0.1088761122278683 2307 100-101 -0.12163226152452111 2201 1 0.3353628543022893 2201 2 0.27737957776341204 2201 3 0.30946677267536415 2201 4 0.40859243682538704 2201 5 0.41509090677325844 2201 6 0.36264893187330216 2201 7 0.4109930494741576 2201 8 0.3773292894987179 2201 9 0.406240315376202 2201 10-11 0.37252350885135144 2201 12-13 0.32262011070837104 2201 14-15 0.5253662138826627 2201 16-17 0.44644217950826004 2201 18-19 0.4824585667016592 2201 20-21 0.5377678619376169 2201 22-23 0.492440732492625 2201 24-25 0.507037633931084 2201 26-27 0.6154867556618555 2201 28-29 0.5554579956397276 2201 30-31 0.5569976262693572 2201 32-33 0.4120779663781775 2201 34-35 0.3782970998070283 2201 36-37 0.41679389726241567 2201 38-39 0.45412613882019315 2201 40-41 0.3848084133312071 2201 42-43 0.4862501236032841 2201 44-45 0.5442912080629654 2201 46-47 0.3724550036467562 2201 48-49 0.5488646221324274 2201 50-51 0.4207841093993494 2201 52-53 0.46535799226366237 2201 54-55 0.5074227471840445 2201 56-57 0.5712380167293247 2201 58-59 0.5613690548967796 2201 60-61 0.5909503366257951 2201 62-63 0.6534537677704648 2201 64-65 0.6128141128971123 2201 66-67 0.5906675181160992 2201 68-69 0.5752789155841143 2201 70-71 0.536669480849497 2201 72-73 0.5035743745287107 2201 74-75 0.5050735345887531 2201 76-77 0.47051339173000883 2201 78-79 0.48401932540662784 2201 80-81 0.5950162393356067 2201 82-83 0.5334656070975967 2201 84-85 0.48804697617445925 2201 86-87 0.39722571508765725 2201 88-89 0.5911849408193532 2201 90-91 0.4091461036707642 2201 92-93 0.47241843827999475 2201 94-95 0.42046402709340924 2201 96-97 0.3762721810804024 2201 98-99 0.4013722787609879 2201 100-101 0.38166101191046664 1101 1 0.2427579903106647 1101 2 0.2703017643212533 1101 3 0.16927473922572034 1101 4 0.2883034432314986 1101 5 0.33187726015932384 1101 6 0.3472294433913632 1101 7 0.372581647161752 1101 8 0.31498811453720066 1101 9 0.354557329563967 1101 10-11 0.3084223995465507 1101 12-13 0.2935562890906098 1101 14-15 0.44464287412886705 1101 16-17 0.3875102264387138 1101 18-19 0.5213589260587028 1101 20-21 0.45168036746641604 1101 22-23 0.40549836429872954 1101 24-25 0.4712674761632698 1101 26-27 0.4634117371351181 1101 28-29 0.5301401900540625 1101 30-31 0.41126839933325954 1101 32-33 0.3118469558515571 1101 34-35 0.25047955316691173 1101 36-37 0.3420206030255386 1101 38-39 0.26607192113684874 1101 40-41 0.3131883171212735 1101 42-43 0.284470001403605 1101 44-45 0.3478062041436729 1101 46-47 0.3177863922103086 1101 48-49 0.24639799029342413 1101 50-51 0.3462444080759539 1101 52-53 0.3542687989777562 1101 54-55 0.3133100048861692 1101 56-57 0.28367552697287834 1101 58-59 0.27228993122316325 1101 60-61 0.39742079906813643 1101 62-63 0.4028242167259677 1101 64-65 0.41000004635314724 1101 66-67 0.3481330430159204 1101 68-69 0.2624291564953509 1101 70-71 0.3267224659614669 1101 72-73 0.2942694691906311 1101 74-75 0.27739070560468093 1101 76-77 0.2806876472368316 1101 78-79 0.3877312143481575 1101 80-81 0.36774395331345033 1101 82-83 0.34024048959663844 1101 84-85 0.22049289519740967 1101 86-87 0.34937215451810033 1101 88-89 0.4359013537445193 1101 90-91 0.28161125299351397 1101 92-93 0.36257100550711385 1101 94-95 0.2702191103193101 1101 96-97 0.20503651179594584 1101 98-99 0.3061215992115436 1101 100-101 0.2931551215708659 2304 1 0.22353759431093323 2304 2 0.19457298478538831 2304 3 0.21055810226653193 2304 4 0.24456090376845196 2304 5 0.25652715815224525 2304 6 0.23410540619099152 2304 7 0.2607785699591503 2304 8 0.2672472295499091 2304 9 0.2871756160634362 2304 10-11 0.307222598887293 2304 12-13 0.2916054228275442 2304 14-15 0.41739613055906943 2304 16-17 0.38156022846968796 2304 18-19 0.3654908438572875 2304 20-21 0.37954675326238174 2304 22-23 0.3437900875086086 2304 24-25 0.46649998285564465 2304 26-27 0.45528353535546984 2304 28-29 0.38050203019100337 2304 30-31 0.36277618668157885 2304 32-33 0.31431521704033827 2304 34-35 0.3854671293118841 2304 36-37 0.3410322415723783 2304 38-39 0.28998669554419365 2304 40-41 0.4020566391081104 2304 42-43 0.38424528446178385 2304 44-45 0.3896841826258779 2304 46-47 0.4407160809216535 2304 48-49 0.42061778136999806 2304 50-51 0.40679896319100095 2304 52-53 0.37044977276863733 2304 54-55 0.4458926948244013 2304 56-57 0.5386229030258676 2304 58-59 0.504064819443407 2304 60-61 0.5319911747800106 2304 62-63 0.5895748272179091 2304 64-65 0.5393147535856215 2304 66-67 0.5561349752758815 2304 68-69 0.583873005431407 2304 70-71 0.6142227007240635 2304 72-73 0.5788181157132826 2304 74-75 0.44117336654154116 2304 76-77 0.5343130587265321 2304 78-79 0.48493865319959895 2304 80-81 0.5498830402157893 2304 82-83 0.5727087900945094 2304 84-85 0.6099835236929181 2304 86-87 0.5186449844709244 2304 88-89 0.571026730074415 2304 90-91 0.5398702885145212 2304 92-93 0.5611383203514819 2304 94-95 0.5697405877119976 2304 96-97 0.5204877342429981 2304 98-99 0.5853411566174458 2304 100-101 0.6021154530250818 2202 1 0.2425547411242306 2202 2 0.21186368233284725 2202 3 0.18288379830357115 2202 4 0.26665178701475156 2202 5 0.3968696253182884 2202 6 0.31041962383957866 2202 7 0.32022452257763234 2202 8 0.26788536712638233 2202 9 0.3341566955673727 2202 10-11 0.34843888587288774 2202 12-13 0.29838727238317375 2202 14-15 0.4214364957937562 2202 16-17 0.40225153608960085 2202 18-19 0.42820581594786944 2202 20-21 0.3852865136413257 2202 22-23 0.4368495932310168 2202 24-25 0.45297664250872316 2202 26-27 0.40424999916339743 2202 28-29 0.46018846928222246 2202 30-31 0.4428384313810554 2202 32-33 0.4722730312129002 2202 34-35 0.35615437274762485 2202 36-37 0.3926929561975001 2202 38-39 0.36754952762948534 2202 40-41 0.4663321770463611 2202 42-43 0.4083345331376833 2202 44-45 0.3254147510738221 2202 46-47 0.39847711578831735 2202 48-49 0.3183820352322897 2202 50-51 0.3679851954334481 2202 52-53 0.3693484799536719 2202 54-55 0.511236729026642 2202 56-57 0.5580250352465157 2202 58-59 0.548291635699016 2202 60-61 0.45494916486502035 2202 62-63 0.4559776521696577 2202 64-65 0.4604733008480366 2202 66-67 0.4987154936393239 2202 68-69 0.5014689818486247 2202 70-71 0.6287715095541415 2202 72-73 0.5793957960225313 2202 74-75 0.4631598750676318 2202 76-77 0.5789275294059664 2202 78-79 0.6139734680931994 2202 80-81 0.5145193806545052 2202 82-83 0.5106427125256161 2202 84-85 0.6528077717885097 2202 86-87 0.6148224675964684 2202 88-89 0.5228652578125761 2202 90-91 0.4463149523215577 2202 92-93 0.600808357123249 2202 94-95 0.4766410467423938 2202 96-97 0.4911887271575708 2202 98-99 0.5396320656642359 2202 100-101 0.578736243612024 1102 1 0.09755225364119724 1102 2 -0.0033259523525188683 1102 3 0.05182658308932986 1102 4 0.07099661658141798 1102 5 0.14911057200543354 1102 6 0.11949944138793711 1102 7 0.07666368867707973 1102 8 0.1068055899365632 1102 9 0.07305883464755425 1102 10-11 0.06562685033564009 1102 12-13 0.10889845984780777 1102 14-15 0.08389474992992518 1102 16-17 0.08505829397174125 1102 18-19 0.12606165214186404 1102 20-21 0.06534062512867678 1102 22-23 0.14404761628350826 1102 24-25 0.05506077298787915 1102 26-27 0.08923953310765143 1102 28-29 0.09057577155449081 1102 30-31 0.05763612256217243 1102 32-33 0.07482093563456971 1102 34-35 0.10763421171704834 1102 36-37 -0.013339481202137904 1102 38-39 -0.029687775147259288 1102 40-41 0.07815113640143068 1102 42-43 0.0738115283823646 1102 44-45 0.10139082622142581 1102 46-47 0.16944873209347833 1102 48-49 0.11183471600944017 1102 50-51 0.09713597415922237 1102 52-53 0.21509806608461446 1102 54-55 0.29446678589336983 1102 56-57 0.26964844971862334 1102 58-59 0.3199942072364692 1102 60-61 0.2554368046743676 1102 62-63 0.16892886257657125 1102 64-65 0.2332456453143834 1102 66-67 0.33311020480800124 1102 68-69 0.3172692722748849 1102 70-71 0.3847354538562513 1102 72-73 0.32875646243514467 1102 74-75 0.2812932771139671 1102 76-77 0.3490445536466993 1102 78-79 0.3273217444343217 1102 80-81 0.37446632469816876 1102 82-83 0.28387793838984976 1102 84-85 0.32214781653992475 1102 86-87 0.27655419041713003 1102 88-89 0.2570914720807167 1102 90-91 0.26514368542821387 1102 92-93 0.382312077482041 1102 94-95 0.37269832792731705 1102 96-97 0.19340759306709998 1102 98-99 0.21736831640252774 1102 100-101 0.3009941719306042 2305 1 0.14403598809350626 2305 2 0.1250340307482105 2305 3 0.1388950609376849 2305 4 0.1743585688767766 2305 5 0.20613558435765356 2305 6 0.22367413401455138 2305 7 0.23785909723126508 2305 8 0.18043127109888957 2305 9 0.09720617337051607 2305 10-11 0.1360076567745523 2305 12-13 0.1118279053942075 2305 14-15 0.15376678808217292 2305 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74-75 -0.46949812985017303 1213 76-77 -0.37676277090678667 1213 78-79 -0.24182848494479714 1213 80-81 -0.3476635718764456 1213 82-83 -0.2837183724481349 1213 84-85 -0.32974566048974907 1213 86-87 -0.34379323766727765 1213 88-89 -0.4213704591261447 1213 90-91 -0.4330957060701053 1213 92-93 -0.2913641494537913 1213 94-95 -0.4274939897189185 1213 96-97 -0.2967359139886483 1213 98-99 -0.354573986293488 1213 100-101 -0.09188417044908093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 532.0 3 256.0 4 116.0 5 168.0 6 400.0 7 1348.0 8 4297.0 9 7665.0 10 6869.0 11 7282.0 12 5963.0 13 5156.0 14 5098.0 15 5414.0 16 5865.0 17 6395.0 18 6924.0 19 8006.0 20 9162.0 21 10738.0 22 12707.0 23 13952.0 24 15794.0 25 18883.0 26 22892.0 27 28370.0 28 34739.0 29 42872.0 30 53580.0 31 71389.0 32 99861.0 33 146005.0 34 224911.0 35 380620.0 36 757270.0 37 1424598.0 38 472343.0 39 1936.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.903522674983385 32.21033795183803 15.65839255585664 19.22774681732195 2 31.311340867039956 24.62659691687662 29.663324439527962 14.398737776555473 3 32.72803048344844 23.327519223358923 24.102458484392873 19.841991808799765 4 28.794085177712674 22.79745606843363 28.54623758877841 19.862221165075283 5 30.917058350825506 21.108886705649834 28.460096763766863 19.513958179757797 6 28.441138601811446 21.39975168719636 30.314671731341996 19.844437979650202 7 31.816726144963603 21.967896531769174 24.469407111959658 21.745970211307565 8 27.882405938080808 25.408656755905064 25.04751169469529 21.66142561131884 9 26.301724397671116 23.14227774512292 28.402872855610102 22.15312500159586 10-11 22.2812633035504 29.00885318369319 31.04327918196608 17.666604330790328 12-13 22.616227126811207 28.30882592352401 30.09597006427585 18.97897688538893 14-15 22.369874097469985 28.101147968254896 30.603034137720186 18.925943796554932 16-17 22.542121436897318 27.791683848884997 30.80899203468531 18.85720267953237 18-19 22.11961523422066 27.773168498881624 30.889187133843716 19.218029133054003 20-21 22.198064882161262 28.234162842203688 30.305947676137652 19.261824599497402 22-23 22.24690814649117 28.256833632585455 30.045185240594364 19.451072980329013 24-25 21.642393190236927 28.48365780564085 30.25595260733543 19.61799639678679 26-27 21.54663642765658 28.653187152560943 30.517798323028124 19.282378096754353 28-29 21.450782625790428 29.10407968785604 30.097956677451453 19.34718100890208 30-31 21.250322120536257 29.39594398341442 30.13576398431131 19.217969911738013 32-33 20.795397891288065 29.480584726069246 30.475090889781146 19.24892649286154 34-35 20.83997897931057 29.783676555387885 30.45786455969017 18.91847990561138 36-37 20.820503474094973 29.305503083000477 30.941799004012683 18.93219443889187 38-39 21.021088264787974 28.861097201208256 30.923337221289643 19.194477312714127 40-41 21.06948972467363 29.29877385207829 30.001752205881505 19.629984217366573 42-43 21.46737761861435 28.85736469027854 30.04788622859967 19.627371462507444 44-45 20.90472262685847 29.014583007970007 30.36267989130671 19.718014473864816 46-47 21.13473177394946 29.247516984111176 30.069958674669856 19.547792567269507 48-49 20.863764606819554 29.640024161819774 29.892877145633246 19.603334085727425 50-51 20.998181151204747 29.320417760042332 29.526869241004494 20.154531847748423 52-53 21.271892715660407 29.12047505384513 29.451648788158725 20.15598344233574 54-55 21.573841271334107 29.095386859657346 29.458752229184437 19.87201963982411 56-57 21.604229799908016 28.820515035620854 29.592714475598946 19.982540688872188 58-59 21.58677018914517 29.258143143280012 29.458132460144622 19.6969542074302 60-61 21.05968269056325 29.52915909677452 29.12426888256893 20.28688933009329 62-63 21.136860280240235 29.481369310518474 29.08674733948578 20.295023069755512 64-65 20.945035453744566 29.35346994965548 29.33874014009799 20.362754456501968 66-67 21.263226190748462 29.005785518150834 29.269961161989528 20.46102712911118 68-69 21.305532931530653 29.10145038631188 29.75857982873879 19.834436853418676 70-71 21.195373553596745 29.90332251332083 29.2877200544369 19.613583878645528 72-73 20.93624804007728 30.106738097090545 29.03123190269355 19.925781960138625 74-75 21.099755451500098 29.589640361437162 28.98691721731808 20.32368696974466 76-77 21.19558614367071 29.32310828021572 29.01238093011496 20.46892464599861 78-79 20.893801349400707 28.996810398252443 29.728194625622617 20.38119362672423 80-81 20.84096701279494 29.410631396640106 29.4205328218832 20.327868768681753 82-83 20.419128909717372 29.66700327225616 29.28201542149435 20.631852396532118 84-85 20.454995332480124 29.68405906185726 29.41846088771691 20.442484717945703 86-87 20.596524342827056 29.47282394429645 29.909309846204373 20.021341866672117 88-89 20.74981845378604 29.97818122516919 29.58084998646551 19.691150334579255 90-91 21.099335299361904 29.69349709152741 29.044155652319358 20.163011956791326 92-93 20.798461522344745 29.7748393543872 29.076261855989888 20.35043726727817 94-95 20.42700676353387 29.92523587283008 29.098360289224402 20.549397074411647 96-97 20.528058944878698 30.30834698957492 28.92517180288709 20.23842226265929 98-99 20.85742161418583 30.063541819078647 28.8369067753022 20.24212979143332 100-101 20.930752677618173 30.072100091530924 28.433323390432168 20.56382384041874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 304.0 1 296.0 2 285.0 3 292.0 4 270.0 5 251.0 6 290.0 7 348.0 8 459.0 9 631.5 10 752.5 11 869.5 12 982.0 13 943.0 14 817.5 15 729.0 16 681.5 17 619.0 18 583.0 19 680.0 20 887.0 21 1166.5 22 1598.5 23 2309.5 24 3299.5 25 5028.5 26 8073.0 27 12423.0 28 17378.5 29 22644.5 30 28553.0 31 33327.5 32 40511.0 33 56095.0 34 78467.5 35 111065.5 36 154260.0 37 205348.0 38 257414.0 39 277865.5 40 283998.0 41 272905.5 42 239792.5 43 225199.0 44 212850.5 45 203154.5 46 207600.5 47 193978.0 48 158603.5 49 128411.5 50 102394.5 51 82876.0 52 68842.0 53 54847.5 54 44512.0 55 34500.5 56 23934.5 57 15422.0 58 10107.0 59 7211.0 60 5372.0 61 4052.0 62 2987.0 63 2084.0 64 1478.5 65 1089.5 66 832.5 67 634.0 68 470.0 69 342.5 70 258.0 71 193.0 72 155.0 73 119.0 74 86.5 75 66.0 76 55.5 77 50.5 78 48.0 79 39.0 80 29.5 81 22.5 82 18.0 83 18.5 84 15.5 85 14.5 86 12.0 87 8.0 88 5.5 89 4.5 90 5.0 91 3.5 92 5.0 93 6.5 94 3.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21875452762694192 2 0.20189389997285973 3 0.17620758825173913 4 0.16011219332023255 5 0.17143763761435127 6 0.13144147398106712 7 0.11891716508824664 8 0.11473389287150008 9 0.10187798313223018 10-11 0.08732580752458438 12-13 0.11528230965601259 14-15 0.11148165380055382 16-17 0.10256669258254819 18-19 0.09589641401743099 20-21 0.10004142459804875 22-23 0.08644578989362245 24-25 0.4031628598889494 26-27 0.19257081463614714 28-29 0.09166212628584605 30-31 0.17489393874465103 32-33 0.20616644934057346 34-35 0.20508236965025803 36-37 0.21115321591602437 38-39 0.21158684779215056 40-41 0.20825808544894672 42-43 0.22479986613528907 44-45 0.20617920321928307 46-47 0.2258074225533469 48-49 0.21474980971212965 50-51 0.21837191126565408 52-53 0.20992884355990343 54-55 0.19156325821808928 56-57 0.20024864961932223 58-59 0.19556797613290156 60-61 0.17462610729174957 62-63 0.1626629690621512 64-65 0.1669227645511553 66-67 0.18035259883235688 68-69 0.1725217173046667 70-71 0.16545606849955208 72-73 0.16930773986984923 74-75 0.15876028217701568 76-77 0.16976687950339456 78-79 0.1674456735782486 80-81 0.17340173493562863 82-83 0.19857789150836552 84-85 0.19637147049160592 86-87 0.20096286682705944 88-89 0.20786271520894933 90-91 0.18905074411229944 92-93 0.16929498599113962 94-95 0.1700729725924248 96-97 0.1791919958697839 98-99 0.19382069474968727 100-101 0.20500584637800048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3920376.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.36209080216989 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4164678828441 37.90560817940853 2 5.877719485986268 5.097404121220398 3 1.8810280523499538 2.4469592762228243 4 1.0115938526237265 1.7545929796954758 5 0.5860061634637364 1.2705226235372873 6 0.3868740433879631 1.0065400439034882 7 0.33199206909309464 1.0077109171930523 8 0.2556754719743737 0.8869298425312349 9 0.20477812696777156 0.7991646962287307 >10 1.6410169122826268 14.269065351820284 >50 0.21032242662570858 6.478436891645541 >100 0.1628090747955577 13.956754907722377 >500 0.02411835422252088 7.336908065489927 >1k 0.009598083382594772 5.78340210338065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.611706632220991E-4 0.0 0.0 2 6.887094503180307E-4 0.0 0.0 3 9.948025393482665E-4 0.0 0.0 4 0.001173356841282571 0.0 0.0 5 0.001658004232247111 0.0 0.0 6 0.0027038222864337502 0.0 0.0 7 0.0033415162219134083 0.0 0.0 8 0.0044128420335192335 0.0 0.0 9 0.005050535968998892 0.0 0.0 10-11 0.007282464743177695 0.0 0.0 12-13 0.01064948872251029 0.0 0.0 14-15 0.015368423845059759 0.0 0.0 16-17 0.02247233428630315 0.0 0.0 18-19 0.031208741202374466 0.0 0.0 20-21 0.04138633641262981 0.0 0.0 22-23 0.053987168577707856 0.0 0.0 24-25 0.06722569467826556 0.0 0.0 26-27 0.08182888580074973 0.0 0.0 28-29 0.09797529624709467 0.0 0.0 30-31 0.11864933363534519 0.0 0.0 32-33 0.13772913618489657 0.0 0.0 34-35 0.15918116017443223 0.0 0.0 36-37 0.1831456982697578 0.0 0.0 38-39 0.21102567712892845 0.0 0.0 40-41 0.24236195711839886 0.0 0.0 42-43 0.27670815248333325 0.0 3.8261636128779486E-5 44-45 0.3131969994714793 0.0 5.101551483837264E-5 46-47 0.3537543337679855 0.0 5.101551483837264E-5 48-49 0.39904335706575084 0.0 5.101551483837264E-5 50-51 0.44863043748864906 0.0 5.101551483837264E-5 52-53 0.4997224755992793 0.0 5.101551483837264E-5 54-55 0.5542325532040804 0.0 6.376939354796581E-5 56-57 0.6113699298230577 0.0 7.652327225755897E-5 58-59 0.6727671019310393 0.0 7.652327225755897E-5 60-61 0.7391893022506004 0.0 7.652327225755897E-5 62-63 0.8103049299352919 0.0 7.652327225755897E-5 64-65 0.8826449299761043 0.0 7.652327225755897E-5 66-67 0.9622036253665465 0.0 7.652327225755897E-5 68-69 1.0479989674459795 0.0 7.652327225755897E-5 70-71 1.137365395564099 0.0 7.652327225755897E-5 72-73 1.232126714376376 0.0 7.652327225755897E-5 74-75 1.332448724306036 0.0 1.0203102967674529E-4 76-77 1.437617208145341 0.0 1.275387870959316E-4 78-79 1.555310000877467 0.0 1.4029266580552479E-4 80-81 1.681509630708891 0.0 1.5304654451511794E-4 82-83 1.8186265807157271 0.0 1.5304654451511794E-4 84-85 1.9650411083018566 0.0 1.5304654451511794E-4 86-87 2.121429169038888 0.0 1.5304654451511794E-4 88-89 2.2887983193448793 0.0 1.5304654451511794E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGG 30 1.3014665E-4 63.334793 1 CGACGCC 90 3.6379788E-12 52.77899 1 GAGCGGC 425 0.0 51.357143 9 CGGGAGA 725 0.0 47.80424 4 CGAGGCG 170 0.0 47.501095 1 CGAGCGG 80 4.2291504E-9 47.50109 1 CGCCGAG 40 5.3891673E-4 47.50109 1 ACCCGCG 50 2.8315011E-5 47.490772 2 TGCCGGC 130 0.0 43.83764 2 CGTGCGG 55 4.964835E-5 43.18281 1 AGTCGCC 45 9.626827E-4 42.223194 1 ACGCGGC 45 9.6492557E-4 42.20323 3 CGAGGGG 350 0.0 42.0724 1 AGAGCGG 540 0.0 40.419975 8 CGAGCGT 60 8.2932864E-5 39.584244 1 CGAGTGG 205 0.0 39.391148 1 CGAGCGC 50 0.0016161083 38.000874 1 CGGAGCG 50 0.0016161083 38.000874 1 ACGGCCC 90 5.722759E-7 36.92783 3 GATCCGG 145 1.6370905E-11 36.035313 1 >>END_MODULE