FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920376
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGG301.3014665E-463.3347931
CGACGCC903.6379788E-1252.778991
GAGCGGC4250.051.3571439
CGGGAGA7250.047.804244
CGAGGCG1700.047.5010951
CGAGCGG804.2291504E-947.501091
CGCCGAG405.3891673E-447.501091
ACCCGCG502.8315011E-547.4907722
TGCCGGC1300.043.837642
CGTGCGG554.964835E-543.182811
AGTCGCC459.626827E-442.2231941
ACGCGGC459.6492557E-442.203233
CGAGGGG3500.042.07241
AGAGCGG5400.040.4199758
CGAGCGT608.2932864E-539.5842441
CGAGTGG2050.039.3911481
CGAGCGC500.001616108338.0008741
CGGAGCG500.001616108338.0008741
ACGGCCC905.722759E-736.927833
GATCCGG1451.6370905E-1136.0353131