##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3146825 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.042650608152663 33.0 33.0 33.0 27.0 33.0 2 30.511649043083107 33.0 33.0 33.0 22.0 33.0 3 30.366562487586695 33.0 33.0 33.0 22.0 33.0 4 33.70374552127939 37.0 37.0 37.0 22.0 37.0 5 33.758906199105446 37.0 37.0 37.0 22.0 37.0 6 34.44305387175963 37.0 37.0 37.0 27.0 37.0 7 33.772879648534634 37.0 37.0 37.0 22.0 37.0 8 34.11204118436837 37.0 37.0 37.0 27.0 37.0 9 33.48721457341924 37.0 37.0 37.0 15.0 37.0 10-11 33.54937897722307 37.0 37.0 37.0 15.0 37.0 12-13 33.53831353824887 37.0 37.0 37.0 18.5 37.0 14-15 35.10956487888586 40.0 37.0 40.0 15.0 40.0 16-17 35.01492424904467 40.0 37.0 40.0 18.5 40.0 18-19 35.058115878703134 40.0 37.0 40.0 18.5 40.0 20-21 35.008423887569215 40.0 37.0 40.0 15.0 40.0 22-23 35.90403708499837 40.0 37.0 40.0 27.0 40.0 24-25 35.17136764834397 40.0 37.0 40.0 22.0 40.0 26-27 34.97982410842675 40.0 37.0 40.0 18.5 40.0 28-29 34.675012274276455 40.0 37.0 40.0 15.0 40.0 30-31 34.62266681496429 40.0 37.0 40.0 15.0 40.0 32-33 34.4097012067719 38.5 37.0 40.0 15.0 40.0 34-35 34.706275690577016 37.0 37.0 40.0 18.5 40.0 36-37 34.43140991316645 37.0 37.0 40.0 15.0 40.0 38-39 34.33680614587719 37.0 37.0 40.0 15.0 40.0 40-41 34.143203387541405 37.0 37.0 40.0 15.0 40.0 42-43 34.055718700595044 37.0 37.0 40.0 15.0 40.0 44-45 33.90266188936468 37.0 35.0 40.0 15.0 40.0 46-47 33.83196523480015 37.0 33.0 40.0 15.0 40.0 48-49 33.72451264369643 37.0 33.0 40.0 15.0 40.0 50-51 32.78603973846655 37.0 33.0 38.5 15.0 38.5 52-53 33.20189969254725 37.0 33.0 38.5 15.0 40.0 54-55 33.83286471284549 37.0 33.0 40.0 15.0 40.0 56-57 34.16655072970375 37.0 33.0 40.0 18.5 40.0 58-59 33.54271829542475 37.0 33.0 40.0 15.0 40.0 60-61 33.34902274514789 37.0 33.0 40.0 15.0 40.0 62-63 32.95124498502459 37.0 33.0 40.0 6.0 40.0 64-65 32.73632423156674 37.0 33.0 38.5 6.0 40.0 66-67 32.5998326885035 37.0 33.0 37.0 10.5 40.0 68-69 32.716528564505495 37.0 33.0 37.0 15.0 40.0 70-71 32.266261231558794 37.0 33.0 37.0 15.0 40.0 72-73 32.60504286066114 37.0 33.0 37.0 18.5 37.0 74-75 32.70648653801848 37.0 33.0 37.0 22.0 37.0 76-77 32.41867501370429 37.0 33.0 37.0 22.0 37.0 78-79 31.91963757120272 37.0 33.0 37.0 22.0 37.0 80-81 31.363016055865835 37.0 33.0 37.0 15.0 37.0 82-83 31.023829256472794 33.0 33.0 37.0 15.0 37.0 84-85 30.76044711733255 33.0 33.0 37.0 15.0 37.0 86-87 30.58474001573014 33.0 33.0 37.0 15.0 37.0 88-89 30.419964408570543 33.0 27.0 37.0 15.0 37.0 90-91 30.290537764254445 33.0 27.0 37.0 10.5 37.0 92-93 30.105542888466946 33.0 27.0 37.0 6.0 37.0 94-95 29.980436948352704 33.0 27.0 37.0 6.0 37.0 96-97 29.855865038570624 33.0 27.0 37.0 6.0 37.0 98-99 29.718114448690347 33.0 27.0 37.0 6.0 37.0 100-101 28.50100212118564 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.052594165731079556 2306 2 0.19863344368961222 2306 3 0.08584983977198135 2306 4 0.3477386965772169 2306 5 0.25650864904518045 2306 6 -0.1006798138131515 2306 7 0.46124854084240496 2306 8 0.016587161911985504 2306 9 0.32734763218789453 2306 10-11 0.2663352560377277 2306 12-13 0.21243058454722785 2306 14-15 0.6025758745422891 2306 16-17 0.5601174246183902 2306 18-19 0.5632247863346365 2306 20-21 0.40867772520602585 2306 22-23 0.25051126417308467 2306 24-25 0.4952738506745362 2306 26-27 0.2872714597878243 2306 28-29 0.37972119248119895 2306 30-31 0.5111067610414608 2306 32-33 0.5638672230039958 2306 34-35 0.26930985400413476 2306 36-37 0.5940451553771027 2306 38-39 0.41585733993424867 2306 40-41 0.46823802317400975 2306 42-43 0.4338779814539748 2306 44-45 0.6005608925545474 2306 46-47 0.501549527513582 2306 48-49 0.5635943552687976 2306 50-51 0.46280622216563927 2306 52-53 0.386100578314192 2306 54-55 0.5187968102465987 2306 56-57 0.4958514805502787 2306 58-59 0.6456303385489903 2306 60-61 0.5985745834168057 2306 62-63 0.7256091301664966 2306 64-65 0.5940952367010439 2306 66-67 0.6320902474054222 2306 68-69 0.4175127642030816 2306 70-71 0.627272952066896 2306 72-73 0.11790201883977147 2306 74-75 0.22193448043064734 2306 76-77 0.4257182333454992 2306 78-79 0.42896005426900885 2306 80-81 0.7767152411450873 2306 82-83 0.6225968827735677 2306 84-85 0.5717762138177314 2306 86-87 0.5582996606900359 2306 88-89 0.5056302557881978 2306 90-91 0.42480657627598717 2306 92-93 0.6255159339932987 2306 94-95 0.3480973894053818 2306 96-97 0.3357379422171647 2306 98-99 0.3751261613252055 2306 100-101 0.4182597258017893 2307 1 0.07332391732502685 2307 2 -0.023327358518102415 2307 3 0.11836592660905865 2307 4 0.3191887791384076 2307 5 0.24852288550385992 2307 6 -0.07510247959384486 2307 7 0.5313486504486917 2307 8 -0.04833131782130806 2307 9 0.3416415082101665 2307 10-11 0.20595151046879323 2307 12-13 0.1854346427364746 2307 14-15 0.5485769563458476 2307 16-17 0.36258199745333997 2307 18-19 0.406995242450769 2307 20-21 0.42702113984574197 2307 22-23 0.12380221894552079 2307 24-25 0.4022358934390553 2307 26-27 0.15290005134234264 2307 28-29 0.40267700679503804 2307 30-31 0.5340276876660894 2307 32-33 0.6789229244794512 2307 34-35 0.37192433933466873 2307 36-37 0.5164601629772889 2307 38-39 0.25768368669886854 2307 40-41 0.4184220153333058 2307 42-43 0.43392608273664734 2307 44-45 0.45934579331476044 2307 46-47 0.5795764623306354 2307 48-49 0.4548079810503225 2307 50-51 0.2712441948011417 2307 52-53 0.43255643588116044 2307 54-55 0.6466850023323261 2307 56-57 0.31048111493299757 2307 58-59 0.5465551950293985 2307 60-61 0.5113748480689253 2307 62-63 0.6411625753495755 2307 64-65 0.6649526087929871 2307 66-67 0.5703970252183765 2307 68-69 0.35605567558549467 2307 70-71 0.43783317991107396 2307 72-73 0.14351357531552367 2307 74-75 0.10819110742801286 2307 76-77 0.19857171360638404 2307 78-79 0.3437124134658127 2307 80-81 0.557505908355509 2307 82-83 0.6441981611649688 2307 84-85 0.6984163392232894 2307 86-87 0.6257172207001886 2307 88-89 0.5447108368440858 2307 90-91 0.4805013974029073 2307 92-93 0.527554439735507 2307 94-95 0.6157961422893194 2307 96-97 0.4048921866800512 2307 98-99 0.34547913538188624 2307 100-101 0.289137060876655 2201 1 0.05035032387789684 2201 2 0.003435760167018742 2201 3 0.03520600890874803 2201 4 0.16570911869496285 2201 5 0.1823674343901871 2201 6 -0.04474061079186242 2201 7 0.4239061870173657 2201 8 -0.04711833220721218 2201 9 0.19737911547980502 2201 10-11 0.18923221380520516 2201 12-13 0.18601267646213415 2201 14-15 0.44935652007805515 2201 16-17 0.39119636283650294 2201 18-19 0.3047967784138663 2201 20-21 0.34456119972563926 2201 22-23 0.1498590725789768 2201 24-25 0.3922379865052079 2201 26-27 0.21644851823342748 2201 28-29 0.31531490717667054 2201 30-31 0.387694731305281 2201 32-33 0.3755768314397656 2201 34-35 0.25465182791174357 2201 36-37 0.5229926803733207 2201 38-39 0.3044022470664558 2201 40-41 0.37986591661007907 2201 42-43 0.4146563063638098 2201 44-45 0.5261900534737762 2201 46-47 0.45433350533280503 2201 48-49 0.40009084694600716 2201 50-51 0.35773903700629006 2201 52-53 0.37374425506340714 2201 54-55 0.39095914270674825 2201 56-57 0.48151332746939346 2201 58-59 0.696897903589381 2201 60-61 0.552675262628398 2201 62-63 0.6644598908474464 2201 64-65 0.5646452672600191 2201 66-67 0.48911201496400736 2201 68-69 0.40853157430095166 2201 70-71 0.5077030375246707 2201 72-73 0.1304774132542903 2201 74-75 0.2305279289291775 2201 76-77 0.4347489144075425 2201 78-79 0.48024071991055095 2201 80-81 0.7283343571783867 2201 82-83 0.6626456024536402 2201 84-85 0.6340481018745514 2201 86-87 0.5470214077425375 2201 88-89 0.5281997713079498 2201 90-91 0.5472495254323704 2201 92-93 0.7354018659673365 2201 94-95 0.6172388688016852 2201 96-97 0.4979786981509662 2201 98-99 0.4493162303053282 2201 100-101 0.3086826586952327 1101 1 -0.2674744572889729 1101 2 -0.3183161814110207 1101 3 -0.26794205275390226 1101 4 -0.09062902671081474 1101 5 -0.1205440731455738 1101 6 -0.2626294984561284 1101 7 0.19327644143807277 1101 8 -0.33585189083175493 1101 9 -0.14853441197815442 1101 10-11 -0.21889200573479428 1101 12-13 -0.10605657500526178 1101 14-15 0.11476296300312328 1101 16-17 -0.042866724076411344 1101 18-19 -0.07054577680170127 1101 20-21 -0.05316697589425701 1101 22-23 -0.14222547759064952 1101 24-25 0.08554646575334601 1101 26-27 -0.1696936047362172 1101 28-29 -0.09215393570023167 1101 30-31 0.021701328171481293 1101 32-33 -0.051664103544538875 1101 34-35 -0.22757683767787285 1101 36-37 0.022629788576431054 1101 38-39 -0.0958230715619166 1101 40-41 0.04671775789114463 1101 42-43 -0.04780783421843182 1101 44-45 -0.014022009569373495 1101 46-47 0.015422703840378915 1101 48-49 -0.025908178872121823 1101 50-51 0.14115547409646467 1101 52-53 -0.04622699982662226 1101 54-55 -0.010905933596760065 1101 56-57 0.13743844081550094 1101 58-59 0.2368823778741529 1101 60-61 0.12858363952327778 1101 62-63 0.1072897098934078 1101 64-65 0.0497266473082405 1101 66-67 0.09326089140592586 1101 68-69 0.04516124059018978 1101 70-71 0.2605719965729989 1101 72-73 -0.2170794086755521 1101 74-75 0.02867421972592865 1101 76-77 0.1867600768670954 1101 78-79 0.4375962454645723 1101 80-81 0.5422259736047188 1101 82-83 0.3141758757065567 1101 84-85 0.34303684614226526 1101 86-87 0.16510189108940665 1101 88-89 0.2880663238539789 1101 90-91 0.4479670345667621 1101 92-93 0.4183437471845899 1101 94-95 0.3778640231252872 1101 96-97 0.2121318236840537 1101 98-99 0.3954737638386874 1101 100-101 0.3532577306608218 2304 1 -0.1208570843645056 2304 2 -0.19094443739798095 2304 3 -0.16049881368913077 2304 4 -0.05274445627605928 2304 5 -0.007540003323533995 2304 6 -0.13262626749305895 2304 7 0.2767078715758302 2304 8 -0.017420806364718544 2304 9 -0.10980894460547574 2304 10-11 -0.08936730532784054 2304 12-13 -0.04537968432358497 2304 14-15 0.13162902915900787 2304 16-17 0.029517743911263494 2304 18-19 0.10728127277093336 2304 20-21 0.032270285026626766 2304 22-23 -0.014852949743776378 2304 24-25 0.04158146111227268 2304 26-27 0.015537774389621006 2304 28-29 0.06980188961958333 2304 30-31 0.054812899236281964 2304 32-33 -0.05378970413761408 2304 34-35 -0.08608683540005302 2304 36-37 0.11818407118583707 2304 38-39 -0.01935788624714263 2304 40-41 0.038975595955150766 2304 42-43 0.1253522687020876 2304 44-45 0.11277236244098532 2304 46-47 -0.015813388008020013 2304 48-49 0.04211621340974858 2304 50-51 0.08613582573372724 2304 52-53 -0.024052654175029886 2304 54-55 0.09377601563494409 2304 56-57 0.06683484330037714 2304 58-59 0.20344716181897837 2304 60-61 0.03974885084566182 2304 62-63 0.26341396824943075 2304 64-65 0.14513215825581227 2304 66-67 0.29238588201952354 2304 68-69 0.24439079463734004 2304 70-71 0.2277081953833644 2304 72-73 0.13582986114716533 2304 74-75 0.3646916352596037 2304 76-77 0.4547940162450672 2304 78-79 0.453939337685906 2304 80-81 0.5173568856126316 2304 82-83 0.3940722936329628 2304 84-85 0.24422557718269644 2304 86-87 0.2939818097838902 2304 88-89 0.2789338758452864 2304 90-91 0.27974445773811496 2304 92-93 0.20221410859312883 2304 94-95 0.29399082423826783 2304 96-97 0.27556075495954246 2304 98-99 0.3265557226018174 2304 100-101 0.24883240862660827 2202 1 -0.01742239283876046 2202 2 -0.10878772553186877 2202 3 0.0032967125107177253 2202 4 0.08028893829681749 2202 5 0.09825222649533316 2202 6 0.06610893346223179 2202 7 0.28006316032824685 2202 8 0.052798490104613904 2202 9 0.14308582033408612 2202 10-11 0.08898764593211439 2202 12-13 0.00736179767334022 2202 14-15 0.10870654074727071 2202 16-17 0.14728297924170164 2202 18-19 0.19012646927131982 2202 20-21 0.22346590425166823 2202 22-23 0.2234093690381087 2202 24-25 0.3045328879690601 2202 26-27 0.20259634958232908 2202 28-29 0.27532007555461036 2202 30-31 0.32990362334785317 2202 32-33 0.2889283742907267 2202 34-35 0.1566960415821015 2202 36-37 0.31022771693901774 2202 38-39 0.1806057872908724 2202 40-41 0.25069259808933 2202 42-43 0.25918950897094817 2202 44-45 0.36018195434509437 2202 46-47 0.31269748477205184 2202 48-49 0.4073045403429987 2202 50-51 0.3272257177729543 2202 52-53 0.2186148617454151 2202 54-55 0.2812745582811118 2202 56-57 0.34053387119934797 2202 58-59 0.46731718181080595 2202 60-61 0.35694820335102406 2202 62-63 0.3831397280063342 2202 64-65 0.41203531509898994 2202 66-67 0.3297254350322234 2202 68-69 0.39855919478804225 2202 70-71 0.5158666278474371 2202 72-73 0.2285758594069378 2202 74-75 0.28443531279843626 2202 76-77 0.4815068518813277 2202 78-79 0.3388445882096356 2202 80-81 0.5303632316982707 2202 82-83 0.4853449838651933 2202 84-85 0.40144490924214793 2202 86-87 0.5008929191768594 2202 88-89 0.4337158986646834 2202 90-91 0.5294962473686304 2202 92-93 0.6084623827793756 2202 94-95 0.7372393181014267 2202 96-97 0.5396835874908703 2202 98-99 0.5114347100753207 2202 100-101 0.4283102893702697 1102 1 -0.06040910719747217 1102 2 -0.21174907983456137 1102 3 -0.19602812331130792 1102 4 -0.2773966727102035 1102 5 -0.11647434857232497 1102 6 -0.021160886178570593 1102 7 -0.20198726643985765 1102 8 -0.050565029265548844 1102 9 -0.27950686688557624 1102 10-11 -0.17028935590365535 1102 12-13 -0.1815978249538901 1102 14-15 -0.29552790256164485 1102 16-17 -0.29062214292185473 1102 18-19 -0.1635676534410777 1102 20-21 -0.24876578067802058 1102 22-23 -0.0616209825961036 1102 24-25 -0.1729322656106831 1102 26-27 -0.14016834600236905 1102 28-29 -0.2431091386621631 1102 30-31 -0.21601357323043402 1102 32-33 -0.2342061712154333 1102 34-35 -0.2286656230973989 1102 36-37 -0.21445693833454982 1102 38-39 -0.20964277437759193 1102 40-41 -0.29767839681973385 1102 42-43 -0.25007684731891544 1102 44-45 -0.23778486467116267 1102 46-47 -0.1588375083201754 1102 48-49 -0.2334005969608839 1102 50-51 -0.042720605018921276 1102 52-53 -0.11969999565157963 1102 54-55 0.03099659124917764 1102 56-57 0.26054205644130946 1102 58-59 0.09475856033802899 1102 60-61 0.0061218724212182 1102 62-63 -0.003877910480390767 1102 64-65 0.016987386485652678 1102 66-67 0.07691974226740683 1102 68-69 0.07611743775252222 1102 70-71 -0.026859270636641952 1102 72-73 0.14994016891316875 1102 74-75 0.263937314161069 1102 76-77 0.3279590462831905 1102 78-79 0.3093883626943885 1102 80-81 0.3438343420523644 1102 82-83 0.2616737845616477 1102 84-85 0.2684625240721026 1102 86-87 0.1587391596431793 1102 88-89 0.05489414768529244 1102 90-91 0.2320963628136603 1102 92-93 0.3525257166804714 1102 94-95 0.3624510461810644 1102 96-97 -0.01739014119438309 1102 98-99 0.14575192841783746 1102 100-101 0.15322997464062027 2305 1 0.0016806073069979277 2305 2 -0.11233693962700642 2305 3 -0.06515518287142186 2305 4 -0.08595918131429414 2305 5 -0.0906301340103326 2305 6 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12729.0 16 11873.0 17 10537.0 18 10173.0 19 10293.0 20 10841.0 21 11949.0 22 12869.0 23 13305.0 24 14802.0 25 16857.0 26 19983.0 27 24175.0 28 30413.0 29 37874.0 30 44426.0 31 56321.0 32 78340.0 33 112820.0 34 172252.0 35 291561.0 36 576717.0 37 994644.0 38 267308.0 39 313.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.43924644921965 22.886681396961926 23.536200794009197 13.137871359809225 2 30.660746241092298 25.998618072051304 31.703146592624197 11.637489094232203 3 36.28020586256919 19.630384561389658 29.530206897166444 14.559202678874703 4 27.33016402083394 17.50829780163957 35.68382542978602 19.477712747740465 5 33.900459978672274 16.134874023142178 33.145872129112355 16.818793869073197 6 30.553460800504013 16.39881378529132 38.09951094098518 14.948214473219485 7 36.208665032219145 18.899630372217533 28.55551193352427 16.336192662039057 8 27.495892830555558 32.75955974706708 24.0460613803907 15.698486041986662 9 32.87423290636082 27.237565862217707 23.024610616079734 16.863590615341746 10-11 27.173782882438317 29.783163599995355 26.74513855084375 16.297914966722576 12-13 29.36312759940533 23.764335005584954 26.788338375327324 20.084199019682387 14-15 25.564590042524195 24.264734186404667 29.237570811357305 20.933104959713837 16-17 27.0942249742895 25.565320898558593 30.090019362799442 17.250434764352473 18-19 26.69822018439087 25.73284794353447 30.47255527829598 17.09637659377868 20-21 25.433557637054214 28.31505646877585 29.067904559824427 17.183481334345508 22-23 28.83991905505926 25.60911863045613 28.32368434653658 17.22727796794803 24-25 24.975013755396272 29.67754862486717 28.125506006350214 17.22193161338634 26-27 22.62978261800522 31.860808278501885 28.059086944360466 17.45032215913243 28-29 23.90221133737804 30.18035798061402 27.772011990998802 18.145418691009134 30-31 26.727852566852178 26.841271005927332 28.241270910430472 18.18960551679002 32-33 22.919988218342766 29.250463703739083 30.229423892882444 17.600124185035703 34-35 27.59796821062238 26.847972111296254 28.442742724606372 17.111316953474994 36-37 24.329896546102074 27.25409000820294 30.03320345338843 18.382809992306555 38-39 20.715491694747833 29.964697662836247 31.46831100957806 17.85149963283786 40-41 21.89172101589742 31.19437539395663 28.71537221746613 18.198531372679824 42-43 24.629614826171604 27.40143259020092 30.383304346275224 17.585648237352252 44-45 22.1380671867537 29.243655468874387 31.087661200445787 17.530616143926125 46-47 23.986272102133725 30.086386963083182 28.301941775865252 17.62539915891784 48-49 20.861108625448253 29.68722683140189 30.142448119262568 19.30921642388729 50-51 19.718757722674155 27.383440166312962 31.948846280250592 20.94895583076229 52-53 25.484243227855114 26.78385371886826 27.977345938241555 19.754557115035073 54-55 27.480759272297984 26.792889427441597 27.486935789846857 18.23941551041356 56-57 28.072411511042883 26.796544770358473 26.847934776084927 18.28310894251372 58-59 26.23320930598332 26.56848590957927 27.425517922835596 19.77278686160181 60-61 21.745612421143907 26.803113944310358 27.2993765362972 24.151897098248533 62-63 24.624929480074194 26.884336181715028 27.45282425837705 21.037910079833726 64-65 21.832242864152278 28.46577344267362 29.929316716589366 19.77266697658474 66-67 20.325960278266695 28.333433610192966 28.62640120994377 22.714204901596567 68-69 20.006028956498724 27.083813795646705 33.92531065121484 18.98484659663973 70-71 19.333362616348264 31.87416388239305 30.531649050705134 18.26082445055356 72-73 19.138964782342775 35.815368905846725 26.61254115474726 18.433125157063245 74-75 19.831576816621055 35.479245243384995 26.114552780877837 18.574625159116113 76-77 20.111729176192686 34.86842513618396 26.427414948587742 18.592430739035613 78-79 19.914672796737786 34.72169746609368 26.970838130408946 18.392791606759587 80-81 19.12177348939826 34.87509044208953 26.92466973716168 19.07846633135053 82-83 18.72289935742513 34.150554800510136 27.176922769546568 19.949623072518165 84-85 18.96769493663511 33.020045676595316 27.708079879596674 20.304179507172908 86-87 19.1737577869655 32.77095774302576 28.328697903151085 19.726586566857655 88-89 19.674408163200322 33.030137544639516 27.70858177290726 19.586872519252907 90-91 20.075971870382688 32.30630324883772 27.377061862808738 20.24066301797085 92-93 19.709447284262673 32.68534230446395 27.442400178505892 20.16281023276748 94-95 19.341293615953926 33.15272455378586 27.521305246981075 19.98467658327914 96-97 19.992324920404076 33.455213554871804 26.788542643725894 19.763918880998226 98-99 20.58724409396173 32.31603172937983 26.48176487153498 20.614959305123463 100-101 20.51084672035113 32.35187962215709 26.043878521062574 21.09339513642921 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 616.0 1 501.0 2 374.5 3 419.5 4 420.5 5 370.5 6 434.0 7 577.5 8 740.0 9 851.0 10 924.0 11 1017.0 12 1064.0 13 1041.5 14 995.0 15 973.0 16 995.5 17 991.5 18 990.5 19 1015.0 20 1089.0 21 1242.5 22 1420.0 23 1680.5 24 2190.0 25 2990.0 26 4506.0 27 7079.5 28 9592.5 29 11609.5 30 13949.5 31 16385.0 32 19853.0 33 29230.5 34 46680.0 35 77302.5 36 116700.5 37 157567.0 38 203144.0 39 234020.0 40 239211.0 41 218183.5 42 192422.5 43 186807.5 44 179796.5 45 167329.5 46 164410.5 47 151634.5 48 123431.5 49 101697.5 50 86101.5 51 74618.0 52 64593.5 53 52444.0 54 41404.0 55 32234.0 56 24166.0 57 17669.5 58 13316.5 59 10137.0 60 7831.0 61 5959.5 62 4412.5 63 3308.0 64 2512.5 65 1910.0 66 1403.5 67 1053.5 68 827.0 69 620.0 70 465.0 71 362.5 72 273.5 73 197.0 74 143.5 75 118.0 76 93.5 77 69.0 78 63.0 79 53.5 80 41.5 81 41.5 82 38.5 83 30.5 84 19.5 85 14.5 86 21.0 87 19.5 88 15.5 89 13.5 90 8.0 91 6.0 92 5.5 93 6.0 94 6.5 95 6.5 96 5.0 97 2.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21644038038340233 2 0.19972511976357124 3 0.17497000945397345 4 0.15981187387287185 5 0.17080708333002312 6 0.12886004147037092 7 0.11640304116053482 8 0.11252611759471848 9 0.09981489278876328 10-11 0.08664288608359219 12-13 0.1130663446489716 14-15 0.109252977207185 16-17 0.10067290046316525 18-19 0.09406306356406854 20-21 0.09840076902910076 22-23 0.08619799321538377 24-25 0.40041947041859655 26-27 0.1926862790272735 28-29 0.08897857364168646 30-31 0.17034630143080723 32-33 0.20179069379453893 34-35 0.2015364692984198 36-37 0.20620784441460838 38-39 0.20728829852311456 40-41 0.2036814884844248 42-43 0.21984063301899534 44-45 0.20099624224416673 46-47 0.2205874174763452 48-49 0.2095445409261716 50-51 0.2135803548020624 52-53 0.2053180586781915 54-55 0.18737934267078724 56-57 0.19500607755436036 58-59 0.19020759019011227 60-61 0.1697901853455467 62-63 0.15833419398917958 64-65 0.16316445941544255 66-67 0.17547845844621165 68-69 0.16904340088819683 70-71 0.16117833053951205 72-73 0.16449913802006783 74-75 0.15439371429933346 76-77 0.16557959212857404 78-79 0.16378413162473285 80-81 0.16880506542308518 82-83 0.19389384538383928 84-85 0.19219371906604277 86-87 0.19575286201171022 88-89 0.20306181627513445 90-91 0.18412209131426122 92-93 0.16499169798129862 94-95 0.16613570821383458 96-97 0.17444567143072778 98-99 0.18909535801959118 100-101 0.20063079453099555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3146825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.33438040385107 #Duplication Level Percentage of deduplicated Percentage of total 1 89.42212816888275 43.221631594367054 2 4.834600354979311 4.673548253163269 3 1.5611011272167787 2.2636456719532947 4 0.9572899288486881 1.8508006231099203 5 0.5355047155233761 1.2941644314081455 6 0.3219238711135462 0.9335994508489478 7 0.3511115539493602 1.1879531588942958 8 0.21366051505233205 0.8261718889457733 9 0.14708614227019753 0.6398385797360417 >10 1.3404041551370767 12.76931787952902 >50 0.15917913233146447 5.389604712714264 >100 0.13126951649235166 13.563784941339673 >500 0.019683302509893235 6.488712656428873 >1k 0.004793622493073783 3.8340236223782167 >5k 1.979199030578525E-4 0.5403066505853162 >10k+ 6.59732966266346E-5 0.5228958845979363 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 16398 0.5210966609201337 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5760 0.183041637205755 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5637 0.17913293557792379 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5547 0.17627290999658385 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4903 0.15580783805899598 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4701 0.1493886695319886 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3937 0.12511023015261413 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3760 0.11948551317597896 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3469 0.11023809712964656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005434048604545852 0.0 0.0 2 0.005878941472754284 0.0 0.0 3 0.006673393023126485 0.0 0.0 4 0.007118285891334917 0.0 0.0 5 0.008389408371930437 0.0 0.0 6 0.009342750232377078 0.0 0.0 7 0.010836319147076815 0.0 0.0 8 0.012520556433865881 0.0 0.0 9 0.014363684030729386 0.0 0.0 10-11 0.023960658759225567 0.0 0.0 12-13 0.032922072247423995 0.0 0.0 14-15 0.043933170735582686 0.0 0.0 16-17 0.055754609805121036 0.0 0.0 18-19 0.06736949147156261 0.0 0.0 20-21 0.07637857205278337 0.0 0.0 22-23 0.08599143581228699 0.0 0.0 24-25 0.09763809554074346 0.0 0.0 26-27 0.11017454100561677 0.0 0.0 28-29 0.12325121352474319 0.0 0.0 30-31 0.21963407561589857 0.0 0.0 32-33 0.23782701611942197 0.0 0.0 34-35 0.25107846797963024 0.0 0.0 36-37 0.26340835604140683 0.0 0.0 38-39 0.2779944865062404 0.0 0.0 40-41 0.2942330761958482 0.0 0.0 42-43 0.31090066972265695 0.0 0.0 44-45 0.32930016762927716 0.0 0.0 46-47 0.34912967832656727 0.0 0.0 48-49 0.36919752448896903 0.0 0.0 50-51 0.3915375020854353 0.0 0.0 52-53 0.4160701659609289 0.0 0.0 54-55 0.44107950076664576 0.0 0.0 56-57 0.46971153464205984 0.0 0.0 58-59 0.5017120430910521 0.0 0.0 60-61 0.5346182263074686 0.0 0.0 62-63 0.5705909925083219 0.0 0.0 64-65 0.6094714513835373 0.0 0.0 66-67 0.6530550634369563 0.0 0.0 68-69 0.6996734804127971 0.0 0.0 70-71 0.7498828183963201 0.0 0.0 72-73 0.803698966418533 0.0 0.0 74-75 0.8636323913786118 0.0 0.0 76-77 0.9265370651370826 0.0 0.0 78-79 0.9952889023062929 0.0 0.0 80-81 1.0710954692428083 0.0 0.0 82-83 1.1545128820318893 0.0 0.0 84-85 1.2439204595107767 0.0 0.0 86-87 1.3426707872220414 0.0 0.0 88-89 1.4475701699331864 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3105 0.0 90.32633 9 CGGGAGA 4250 0.0 89.04159 4 ATTCGGA 775 0.0 87.01619 2 AGAGCGG 3320 0.0 86.33508 8 CGGAAAG 1895 0.0 84.18865 5 AAGCGGC 1135 0.0 84.040504 9 GAGAGCG 3815 0.0 83.59437 7 TCGGGAG 4625 0.0 82.541954 3 TTCGGAG 695 0.0 80.61718 3 TCGGGAA 1485 0.0 79.93629 3 GATCCGG 915 0.0 78.38473 1 TCGGAGA 965 0.0 77.74156 4 GTTCGGA 725 0.0 76.64101 2 AAGGGGC 3055 0.0 75.02818 9 ATCCGGA 945 0.0 74.88035 2 GATCGGG 6445 0.0 74.13968 1 ATCGGGA 6340 0.0 73.8586 2 AAAGCGG 1455 0.0 72.406654 8 CGGAGAG 1795 0.0 72.21405 5 AAGCGTC 395 0.0 72.08463 9 >>END_MODULE