FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146825
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT163980.5210966609201337Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC57600.183041637205755No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56370.17913293557792379No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG55470.17627290999658385No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG49030.15580783805899598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47010.1493886695319886No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA39370.12511023015261413No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.11948551317597896No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA34690.11023809712964656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC31050.090.326339
CGGGAGA42500.089.041594
ATTCGGA7750.087.016192
AGAGCGG33200.086.335088
CGGAAAG18950.084.188655
AAGCGGC11350.084.0405049
GAGAGCG38150.083.594377
TCGGGAG46250.082.5419543
TTCGGAG6950.080.617183
TCGGGAA14850.079.936293
GATCCGG9150.078.384731
TCGGAGA9650.077.741564
GTTCGGA7250.076.641012
AAGGGGC30550.075.028189
ATCCGGA9450.074.880352
GATCGGG64450.074.139681
ATCGGGA63400.073.85862
AAAGCGG14550.072.4066548
CGGAGAG17950.072.214055
AAGCGTC3950.072.084639
Produced by FastQC (version 0.11.2)