##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1850838 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.573637455033882 33.0 33.0 33.0 27.0 33.0 2 31.315514918107365 33.0 33.0 33.0 27.0 33.0 3 31.249751734079375 33.0 33.0 33.0 27.0 33.0 4 34.853017389960655 37.0 37.0 37.0 33.0 37.0 5 34.84264803294508 37.0 37.0 37.0 33.0 37.0 6 35.16230377807242 37.0 37.0 37.0 33.0 37.0 7 34.85827068603519 37.0 37.0 37.0 33.0 37.0 8 34.89866914338262 37.0 37.0 37.0 33.0 37.0 9 34.67064972731271 37.0 37.0 37.0 33.0 37.0 10-11 34.64053850201908 37.0 37.0 37.0 33.0 37.0 12-13 34.6248807297019 37.0 37.0 37.0 33.0 37.0 14-15 36.25268527013169 40.0 37.0 40.0 33.0 40.0 16-17 36.125772217773786 40.0 37.0 40.0 30.0 40.0 18-19 36.044336133146174 40.0 37.0 40.0 27.0 40.0 20-21 35.959891141201986 40.0 37.0 40.0 27.0 40.0 22-23 36.31818776143563 40.0 37.0 40.0 27.0 40.0 24-25 35.83625174110322 40.0 37.0 40.0 27.0 40.0 26-27 35.641810088187086 37.0 37.0 40.0 27.0 40.0 28-29 35.44965442680559 37.0 37.0 40.0 27.0 40.0 30-31 35.38419488901784 37.0 37.0 40.0 27.0 40.0 32-33 35.22785219451946 37.0 37.0 40.0 27.0 40.0 34-35 35.14139919323031 37.0 37.0 40.0 27.0 40.0 36-37 34.973431764422386 37.0 37.0 40.0 22.0 40.0 38-39 34.85883313396418 37.0 37.0 40.0 22.0 40.0 40-41 34.746641791447985 37.0 35.0 40.0 22.0 40.0 42-43 34.50233191667775 37.0 33.0 40.0 22.0 40.0 44-45 34.28527915463158 37.0 33.0 40.0 22.0 40.0 46-47 34.114071571904184 37.0 33.0 40.0 22.0 40.0 48-49 33.92598271701792 37.0 33.0 40.0 22.0 40.0 50-51 32.72761878673336 35.0 33.0 38.5 18.5 38.5 52-53 33.18327346855857 37.0 33.0 37.0 22.0 40.0 54-55 33.83150740367336 37.0 33.0 40.0 22.0 40.0 56-57 33.88372158989604 37.0 33.0 40.0 22.0 40.0 58-59 33.51763309376618 37.0 33.0 38.5 22.0 40.0 60-61 33.25946976450667 37.0 33.0 37.0 22.0 40.0 62-63 32.98997319052235 37.0 33.0 37.0 15.0 40.0 64-65 32.72618727300823 37.0 33.0 37.0 15.0 40.0 66-67 32.50706301686047 37.0 33.0 37.0 15.0 38.5 68-69 32.40468992964268 37.0 33.0 37.0 15.0 37.0 70-71 32.09798156294608 37.0 33.0 37.0 15.0 37.0 72-73 32.06528340135658 37.0 33.0 37.0 18.5 37.0 74-75 31.98392241784532 37.0 33.0 37.0 22.0 37.0 76-77 31.768501349118615 33.0 33.0 37.0 22.0 37.0 78-79 31.521704492775704 33.0 33.0 37.0 15.0 37.0 80-81 31.257815378763567 33.0 33.0 37.0 15.0 37.0 82-83 30.97457935270402 33.0 33.0 37.0 15.0 37.0 84-85 30.809910429762084 33.0 33.0 37.0 15.0 37.0 86-87 30.660810670625956 33.0 27.0 37.0 15.0 37.0 88-89 30.459908430667625 33.0 27.0 37.0 15.0 37.0 90-91 30.327595662073072 33.0 27.0 37.0 15.0 37.0 92-93 30.12938706683135 33.0 27.0 37.0 15.0 37.0 94-95 29.96550724590699 33.0 27.0 37.0 15.0 37.0 96-97 29.800874252635833 33.0 27.0 37.0 15.0 37.0 98-99 29.62908963399282 33.0 27.0 37.0 15.0 37.0 100-101 28.216070504279685 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16392718642508441 2306 2 0.17740564392599012 2306 3 0.26102902339299305 2306 4 0.36244337467156384 2306 5 0.4007890031082937 2306 6 0.1980856691327446 2306 7 0.5031523869820731 2306 8 0.18670826587012357 2306 9 0.3399306514877054 2306 10-11 0.37386247168743836 2306 12-13 0.3096598644939377 2306 14-15 0.7957110772014815 2306 16-17 0.668619561051436 2306 18-19 0.5859752023278588 2306 20-21 0.597459064079338 2306 22-23 0.513479082223256 2306 24-25 0.6497126049586015 2306 26-27 0.6028821021653599 2306 28-29 0.753110761887875 2306 30-31 0.5369174664105216 2306 32-33 0.5288487754773854 2306 34-35 0.5330741600099955 2306 36-37 0.5826791970241842 2306 38-39 0.4855639441305115 2306 40-41 0.6215064188088277 2306 42-43 0.5169501281389657 2306 44-45 0.7525987970870034 2306 46-47 0.7164409474639868 2306 48-49 0.7564282841007568 2306 50-51 0.7315844332248957 2306 52-53 0.7715648232010608 2306 54-55 0.8492686862401015 2306 56-57 0.6059190114718191 2306 58-59 0.8606207390497147 2306 60-61 0.8712927955718541 2306 62-63 1.0471785447354094 2306 64-65 1.0012058272254833 2306 66-67 0.833169623483009 2306 68-69 0.8390483099320107 2306 70-71 1.0194492362402414 2306 72-73 0.7488254446014935 2306 74-75 0.7225111188838085 2306 76-77 0.5856742416494818 2306 78-79 0.6195963548759806 2306 80-81 0.7630666911608586 2306 82-83 0.9092100193190298 2306 84-85 0.8553839184846375 2306 86-87 0.6275684800298684 2306 88-89 0.7238460039483847 2306 90-91 0.5663473150116083 2306 92-93 0.8376924077554548 2306 94-95 0.7319041821054064 2306 96-97 0.6191338603035952 2306 98-99 0.5736580295307512 2306 100-101 0.5809135943354846 2307 1 0.05440141131667531 2307 2 0.09830168548154106 2307 3 0.08253633967432705 2307 4 0.13858325362247115 2307 5 0.0637094863770784 2307 6 0.04649046471747198 2307 7 0.36599495359385514 2307 8 0.024907370756146463 2307 9 0.3258458243652882 2307 10-11 0.1384254659132651 2307 12-13 0.1526960632741705 2307 14-15 0.41849033652945167 2307 16-17 0.3633147353203867 2307 18-19 0.484986402847305 2307 20-21 0.41256167753387984 2307 22-23 0.22198938994767303 2307 24-25 0.422371278984059 2307 26-27 0.09652053177369169 2307 28-29 0.24141584270321914 2307 30-31 0.3186708230879347 2307 32-33 0.3442074008049403 2307 34-35 0.2588063678805028 2307 36-37 0.33911010396479924 2307 38-39 0.18633360301324586 2307 40-41 0.2239647357162724 2307 42-43 0.2638598978674267 2307 44-45 0.40488736699169436 2307 46-47 0.3798340323606766 2307 48-49 0.48739783631187805 2307 50-51 0.184216439346919 2307 52-53 0.3447965093082388 2307 54-55 0.4642140750117534 2307 56-57 0.22230678597730247 2307 58-59 0.391996802867169 2307 60-61 0.30541101554727135 2307 62-63 0.4225638379664005 2307 64-65 0.3587224656782837 2307 66-67 0.4802340670514056 2307 68-69 0.2030496549219194 2307 70-71 0.2198325583653329 2307 72-73 5.96889074280682E-4 2307 74-75 0.0891460932594299 2307 76-77 0.17069917034182325 2307 78-79 0.08264901818790094 2307 80-81 0.215449410359426 2307 82-83 0.46533969489818716 2307 84-85 0.5411316633075849 2307 86-87 0.6747866698589569 2307 88-89 0.6316388291228847 2307 90-91 0.6524888172262067 2307 92-93 0.49060245575768135 2307 94-95 0.47702511525013236 2307 96-97 0.37665165823045754 2307 98-99 0.2966295296582935 2307 100-101 0.11907721931519433 2201 1 0.2073547588296769 2201 2 0.07889399248019657 2201 3 0.03706868201598468 2201 4 0.09575891836735906 2201 5 0.0817542443035677 2201 6 0.030790690871377535 2201 7 0.3258323345333167 2201 8 0.11548423145637088 2201 9 0.07625463759338658 2201 10-11 0.13362760246427996 2201 12-13 0.13161588114871847 2201 14-15 0.3238210815722127 2201 16-17 0.312248187031976 2201 18-19 0.3007878360376921 2201 20-21 0.3137892612222615 2201 22-23 0.39141908820425897 2201 24-25 0.36681642129592973 2201 26-27 0.3113449854668673 2201 28-29 0.34454781187522343 2201 30-31 0.36910099105914185 2201 32-33 0.3489626444935183 2201 34-35 0.2524100391018038 2201 36-37 0.28756038218210733 2201 38-39 0.0825327279174104 2201 40-41 0.22520474642953303 2201 42-43 0.3563310947603995 2201 44-45 0.3235145800227599 2201 46-47 0.20103987548496605 2201 48-49 0.24654123296321728 2201 50-51 0.31369549577234324 2201 52-53 0.45136722020136233 2201 54-55 0.5132476607288439 2201 56-57 0.45699743156797723 2201 58-59 0.5329726518191436 2201 60-61 0.5089008561179682 2201 62-63 0.3737061148396137 2201 64-65 0.44667821111359274 2201 66-67 0.4842984219923636 2201 68-69 0.42396492100605343 2201 70-71 0.4988955337719361 2201 72-73 0.34030666196478876 2201 74-75 0.47145409236038205 2201 76-77 0.4349371985632864 2201 78-79 0.4770401679927687 2201 80-81 0.6448430478124152 2201 82-83 0.4980039279017632 2201 84-85 0.6739411175368772 2201 86-87 0.5773540842257638 2201 88-89 0.6068312124782125 2201 90-91 0.5908776915844989 2201 92-93 0.5878679270671796 2201 94-95 0.4546607324493124 2201 96-97 0.4369406740414874 2201 98-99 0.5729886177389822 2201 100-101 0.1722666803008437 1101 1 0.020408402685962557 1101 2 0.06373372589139592 1101 3 -0.00437364268610807 1101 4 0.09880120214759813 1101 5 0.22653319171686093 1101 6 0.24555069061561596 1101 7 0.3424649351808142 1101 8 0.20703877154380024 1101 9 0.11824727592308903 1101 10-11 0.0767654131879354 1101 12-13 0.09813072491037644 1101 14-15 0.3287715597397636 1101 16-17 0.23036463100847016 1101 18-19 0.25480190718629814 1101 20-21 0.21103513546857045 1101 22-23 0.15319594902947387 1101 24-25 0.4232985915180336 1101 26-27 0.23479022158807794 1101 28-29 0.30730487888688884 1101 30-31 0.3078825429776728 1101 32-33 0.24969156494527311 1101 34-35 0.26866411263751644 1101 36-37 0.23158632492988573 1101 38-39 0.2635643848047451 1101 40-41 0.4815251474636639 1101 42-43 0.458583927852537 1101 44-45 0.5132862488882637 1101 46-47 0.4035842767578046 1101 48-49 0.4921669642814379 1101 50-51 0.4815513826575284 1101 52-53 0.4304972898750705 1101 54-55 0.4907372331168247 1101 56-57 0.5698839778704112 1101 58-59 0.5917400515979168 1101 60-61 0.5954769974006595 1101 62-63 0.6418640135026195 1101 64-65 0.44386419452745685 1101 66-67 0.38045837527324977 1101 68-69 0.25352115338247927 1101 70-71 0.4310466803297004 1101 72-73 0.3691030693673838 1101 74-75 0.46599464868440066 1101 76-77 0.5017555460451995 1101 78-79 0.48216989238837016 1101 80-81 0.531933056700133 1101 82-83 0.5393102727067145 1101 84-85 0.3692651567792282 1101 86-87 0.4942215592410584 1101 88-89 0.6390107059424359 1101 90-91 0.6542089433028941 1101 92-93 0.4904146728210037 1101 94-95 0.4564772789435665 1101 96-97 0.23379288861691094 1101 98-99 0.44021085535425897 1101 100-101 0.4738705184293508 2304 1 0.11966894699443031 2304 2 0.048035992142544615 2304 3 0.055611756596416484 2304 4 0.1562271584553443 2304 5 0.14621982578049142 2304 6 0.1633368764529166 2304 7 0.2679333748665087 2304 8 0.0790246261175156 2304 9 0.07301291870790294 2304 10-11 0.1421840848366429 2304 12-13 0.2320630670695536 2304 14-15 0.40670701043879376 2304 16-17 0.27827303898077815 2304 18-19 0.4342582659557479 2304 20-21 0.3029466519807187 2304 22-23 0.2560542749708645 2304 24-25 0.41691882371653577 2304 26-27 0.3254098493482829 2304 28-29 0.3651038992258435 2304 30-31 0.338550525802205 2304 32-33 0.3278562312089761 2304 34-35 0.3824027205454499 2304 36-37 0.47227463886100907 2304 38-39 0.35316667225048803 2304 40-41 0.38664324842841324 2304 42-43 0.4216260579873179 2304 44-45 0.2912576126423687 2304 46-47 0.40224116774696483 2304 48-49 0.31132746227580554 2304 50-51 0.3263675392148997 2304 52-53 0.3289375250565243 2304 54-55 0.5907594089377213 2304 56-57 0.650464042396159 2304 58-59 0.782840090062173 2304 60-61 0.6609039113898731 2304 62-63 0.7564592360850639 2304 64-65 0.7598659136441981 2304 66-67 0.7365064869922904 2304 68-69 0.7855347964184887 2304 70-71 0.880432037223045 2304 72-73 0.7552149218530388 2304 74-75 0.6234282585940534 2304 76-77 0.8114181203243866 2304 78-79 0.8328086737779472 2304 80-81 0.7771923371830525 2304 82-83 0.6908146960961226 2304 84-85 0.8050789112830792 2304 86-87 0.8262120452251516 2304 88-89 0.8252397174110655 2304 90-91 0.7770759433264445 2304 92-93 0.8067333295549979 2304 94-95 0.5631525935262296 2304 96-97 0.6614400536442702 2304 98-99 0.8144929867450195 2304 100-101 0.8616074857190341 2202 1 0.030182407982952242 2202 2 -0.10038942528524686 2202 3 -0.15512872908468722 2202 4 -0.06520508138544301 2202 5 -0.08402056360478838 2202 6 0.038414934428836034 2202 7 0.24034462221837316 2202 8 0.0734336324030096 2202 9 -0.10304502219564426 2202 10-11 -0.0905592663746475 2202 12-13 -0.0661796801043053 2202 14-15 0.10835994279243266 2202 16-17 0.04056607591751771 2202 18-19 0.07270466420025201 2202 20-21 0.15331826630720258 2202 22-23 0.24020485829009175 2202 24-25 0.08418758243423241 2202 26-27 0.12288660703881504 2202 28-29 0.01921077007855132 2202 30-31 0.054304230273245935 2202 32-33 0.12032268816474812 2202 34-35 0.04501207466311996 2202 36-37 -0.04429188987891308 2202 38-39 0.013817281832210426 2202 40-41 0.03048586020451438 2202 42-43 0.11790393270936761 2202 44-45 0.1217010986678062 2202 46-47 0.030123476745664846 2202 48-49 0.06518002462004802 2202 50-51 0.08069574455260664 2202 52-53 0.19110245937110193 2202 54-55 0.28271491443242525 2202 56-57 0.38092474494713 2202 58-59 0.49045242059612093 2202 60-61 0.27981028267154073 2202 62-63 0.31645080290561367 2202 64-65 0.44473273360602406 2202 66-67 0.39134433066623586 2202 68-69 0.3002359583880576 2202 70-71 0.4657318965727839 2202 72-73 0.35663484750099883 2202 74-75 0.4494093087437463 2202 76-77 0.3803330998688246 2202 78-79 0.26514553990341483 2202 80-81 0.4134516530741976 2202 82-83 0.4053063417041578 2202 84-85 0.41895382592996455 2202 86-87 0.46236412258863524 2202 88-89 0.44001849964827855 2202 90-91 0.429312340812249 2202 92-93 0.3809817844447849 2202 94-95 0.2959696256306721 2202 96-97 0.2777070440205236 2202 98-99 0.3624286086117543 2202 100-101 0.3045981712869157 1102 1 -0.0391466585352056 1102 2 -0.15730373317941826 1102 3 -0.050062624388800714 1102 4 -0.07708297227929961 1102 5 0.0830462048804037 1102 6 0.09406002653663847 1102 7 0.16177348495883592 1102 8 0.06578423410270062 1102 9 0.011275458657827642 1102 10-11 -0.10618591442145231 1102 12-13 0.056138090744937585 1102 14-15 -0.05455392351308319 1102 16-17 -0.0370626422518896 1102 18-19 -0.07821458554906968 1102 20-21 -0.040768207719928284 1102 22-23 0.011064472476618903 1102 24-25 -0.03563640792395262 1102 26-27 0.05978355374862332 1102 28-29 0.01974394618383002 1102 30-31 0.09966322496996582 1102 32-33 0.041902204357974426 1102 34-35 0.07902565140461348 1102 36-37 -0.03562606419955472 1102 38-39 -0.030939802220110835 1102 40-41 -0.13179445295777725 1102 42-43 -0.02738277645668319 1102 44-45 -0.04782218247649439 1102 46-47 0.010309998728764924 1102 48-49 0.004375447286484757 1102 50-51 0.18327896784035858 1102 52-53 0.13660616576245843 1102 54-55 0.09014024868267967 1102 56-57 0.270503850491032 1102 58-59 0.41623230271726186 1102 60-61 0.34465103652242846 1102 62-63 0.30706053979471903 1102 64-65 0.34785922344034503 1102 66-67 0.23608953120233167 1102 68-69 0.3569227918739841 1102 70-71 0.37059784488440783 1102 72-73 0.48433676531769265 1102 74-75 0.4657544173056465 1102 76-77 0.4013458285913565 1102 78-79 0.1487064179616624 1102 80-81 0.2203603356352417 1102 82-83 0.23969653449838546 1102 84-85 0.44622526512925376 1102 86-87 0.33753622262393534 1102 88-89 0.29274516893072544 1102 90-91 0.4079789681015846 1102 92-93 0.29165782291643794 1102 94-95 0.36162693174302163 1102 96-97 0.23916366777996245 1102 98-99 0.2989474274605577 1102 100-101 0.452196540784108 2305 1 0.2445217687837946 2305 2 0.15426371379696135 2305 3 0.09014016803948977 2305 4 0.1656600048684993 2305 5 0.08001785695270058 2305 6 0.16300757360720297 2305 7 0.22866434653840173 2305 8 0.14362443579391027 2305 9 0.13826461360948628 2305 10-11 0.3042272947463758 2305 12-13 0.34182239373260614 2305 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9337.0 16 9524.0 17 8634.0 18 8372.0 19 8631.0 20 9177.0 21 9547.0 22 10230.0 23 11061.0 24 12210.0 25 13903.0 26 16629.0 27 19510.0 28 23230.0 29 28051.0 30 35012.0 31 46383.0 32 64280.0 33 92225.0 34 141267.0 35 243951.0 36 427257.0 37 438986.0 38 60800.0 39 36.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.615262499329965 22.54993315895283 24.435235113180383 12.399569228536823 2 34.4566038246559 22.210504946609102 31.72910717427493 11.60378405446007 3 39.57082093255982 16.441382565148373 27.79602601551037 16.191770486781436 4 32.75131902919451 15.063989826564573 34.88389837387375 17.300792770367163 5 31.806432897921365 14.474507401426164 37.29006923006984 16.428990470582633 6 27.405756785911745 14.620857772569845 42.45529526709232 15.518090174426092 7 38.04147099010447 15.430023248593347 29.216687924687108 17.311817836615074 8 32.52770208213209 28.778144266104423 22.84811913599145 15.84603451577204 9 33.86682603122752 27.27892223406039 20.962947739601194 17.891303995110896 10-11 28.128862148129663 25.57448756366334 27.418341801951414 18.878308486255584 12-13 29.531239393340307 21.79789423238412 26.11685168512524 22.55401468915033 14-15 27.21253495084638 23.535404372401928 26.37990768838841 22.872152988363283 16-17 27.200887826698565 24.98867636969956 27.665271595413653 20.14516420818822 18-19 27.262371037151308 24.909315339261354 27.152292094514845 20.676021529072493 20-21 26.04979096254037 26.427250880915576 27.10864729062521 20.41431086591885 22-23 28.207596934059126 25.546406977181185 25.472216161322848 20.773779927436838 24-25 26.441311503153674 26.816481554206963 25.64877053359688 21.093436409042486 26-27 24.098236109746555 28.253071739187508 26.052316122608115 21.596376028457822 28-29 24.91347591061196 27.557598004754496 25.3282778028938 22.200648281739742 30-31 26.27806122310915 25.904678300222816 26.14752062157243 21.669739855095603 32-33 25.043316883609744 25.287585940128636 27.808640747186132 21.86045642907549 34-35 23.89376530562803 26.31676109564138 26.66323977596905 23.126233822761538 36-37 24.87400062695144 27.665206847955616 26.1671827195291 21.293609805563843 38-39 25.761077714955828 26.746503419834877 24.780199878831784 22.712218986377508 40-41 25.371547336333883 25.90532814431653 24.23325346968353 24.48987104966606 42-43 26.958772878837596 25.584174520818237 25.227718955118977 22.229333645225186 44-45 25.125058739711076 26.444157753750137 25.5591370838684 22.871646422670388 46-47 25.836184238312914 26.713196251642046 25.178797497484812 22.27182201256023 48-49 24.25842014673771 26.575676148327343 25.8465897370232 23.31931396791175 50-51 23.53824362912998 25.703413571336526 25.837799322331968 24.92054347720153 52-53 26.46682311336141 25.487483768136578 24.366378704769712 23.6793144137323 54-55 27.033563658901638 25.253236331354863 25.02835088517012 22.684849124573383 56-57 27.393040406265918 25.069169901632847 24.58935192227636 22.94843776982487 58-59 26.342140056810877 25.05903702991976 24.999167730122622 23.599655183146737 60-61 24.54603979030276 25.215868386379586 24.881129981601564 25.356961841716092 62-63 26.1554208883938 25.018202794964424 25.22464995301501 23.60172636362677 64-65 25.359310634447496 26.166278105727542 25.789812343466224 22.684598916358738 66-67 24.28681028288905 26.063705850595287 25.165096413924303 24.484387452591356 68-69 23.499491545695673 24.48890736173642 28.38371062861621 23.627890463951697 70-71 23.468572702615532 26.63595225674339 26.767941061235444 23.127533979405634 72-73 23.56082554510812 28.569588566861032 25.225272029792922 22.64431385823793 74-75 23.95750361468732 28.62258768322352 25.123970788131704 22.295937913957456 76-77 23.98740887180439 28.21095145656288 24.83652260596198 22.965117065670746 78-79 23.913024066689594 28.411319242501193 24.970824114258534 22.70483257655068 80-81 24.199688377588082 28.86290291592635 24.759234073459112 22.178174633026458 82-83 23.734533792275023 28.501074680336398 25.050377755348567 22.714013772040015 84-85 23.406545014397928 28.522674401137433 24.957823911413026 23.11295667305161 86-87 23.14585495327386 28.744925573270148 24.995520375389834 23.113699098066157 88-89 23.324565585004255 28.72269958943983 25.04059719220913 22.912137633346788 90-91 23.712485554451934 28.175544194581192 24.743606745495832 23.36836350547104 92-93 23.404437822829653 27.819991173385077 24.46195396143727 24.313617042348 94-95 23.449295715574895 28.439723508663693 23.94740224510532 24.16357853065609 96-97 23.3903404596526 29.065164132841208 23.653541866640833 23.89095354086536 98-99 23.610361546610342 27.761643721422196 24.301046515341547 24.326948216625915 100-101 24.345927773589707 28.024892031220606 23.81850147048673 23.810678724702957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2399.0 1 1550.5 2 650.0 3 1036.0 4 1246.0 5 1017.5 6 1319.0 7 1871.5 8 2158.0 9 2056.0 10 1729.5 11 1458.0 12 1329.5 13 1218.0 14 1109.0 15 1029.0 16 1022.0 17 1038.5 18 995.0 19 978.0 20 956.0 21 984.0 22 1064.0 23 1173.0 24 1316.0 25 1504.5 26 1783.5 27 2074.0 28 2446.0 29 3058.5 30 3911.5 31 4976.0 32 6279.0 33 8105.0 34 10783.5 35 15290.0 36 20977.5 37 28307.5 38 36168.0 39 40653.0 40 46351.5 41 50588.5 42 56917.0 43 69356.0 44 76244.0 45 82082.0 46 94669.0 47 101837.5 48 98941.5 49 95792.0 50 92643.0 51 90293.5 52 88132.5 53 85148.5 54 79671.5 55 70257.0 56 61169.0 57 54660.5 58 50047.0 59 42399.5 60 32411.0 61 23963.0 62 17441.5 63 13102.5 64 10392.5 65 8658.5 66 7911.5 67 7183.5 68 6022.5 69 4611.0 70 3230.5 71 2393.5 72 2025.5 73 1657.5 74 1125.5 75 769.5 76 619.5 77 488.0 78 377.0 79 310.0 80 256.0 81 193.5 82 154.0 83 121.0 84 100.0 85 77.0 86 49.5 87 38.5 88 27.5 89 22.0 90 21.5 91 20.5 92 14.5 93 7.0 94 4.5 95 5.5 96 4.5 97 2.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2117419244688082 2 0.19520887295376474 3 0.17132779854314642 4 0.15603742737073692 5 0.1667352842334121 6 0.12837428235210213 7 0.1147588281632428 8 0.11130093503591346 9 0.09876607244934457 10-11 0.08425913018859565 12-13 0.11121989066574169 14-15 0.10759990879806876 16-17 0.09879308723940183 18-19 0.09241759678588833 20-21 0.09549728285241604 22-23 0.08388092312779401 24-25 0.39301116575302647 26-27 0.1876177169476745 28-29 0.08871657054804365 30-31 0.16949079281925267 32-33 0.1996122837330982 34-35 0.19896392877172395 36-37 0.20558255233575276 38-39 0.20633896645735608 40-41 0.2016113781973355 42-43 0.2184956219831233 44-45 0.2002336239044152 46-47 0.21914397694449758 48-49 0.20904044546308215 50-51 0.21157983572846462 52-53 0.20404270930248894 54-55 0.18656414013544137 56-57 0.19315574890941292 58-59 0.18804995358859067 60-61 0.1689234822280502 62-63 0.1570910041829701 64-65 0.16176456286287616 66-67 0.17319181905709738 68-69 0.1664651363328395 70-71 0.15960337965829532 72-73 0.1629802284154529 74-75 0.15247147508317854 76-77 0.1636555981668844 78-79 0.16173754807281893 80-81 0.16689737297375568 82-83 0.19150784671592003 84-85 0.19037322553351507 86-87 0.19369604471055815 88-89 0.2013952598768774 90-91 0.18278206952742487 92-93 0.16303425799556742 94-95 0.1642769383382014 96-97 0.17308375989686833 98-99 0.18742861341727368 100-101 0.1977752780092045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1850838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.18160418940315 #Duplication Level Percentage of deduplicated Percentage of total 1 83.94855697392882 43.80570372285114 2 7.768094715985575 8.107032875507068 3 2.4725393749291937 3.870632130158084 4 1.4724345834730526 3.073359945183181 5 0.8709336077337447 2.2723356397005587 6 0.5249795521931089 1.6436565120042552 7 0.4478100633965182 1.6357213236132053 8 0.30515574485104957 1.2738813035151977 9 0.23411978749074697 1.0995071475374403 >10 1.7300876792605513 17.34239134596755 >50 0.13735295289623767 4.944475671963349 >100 0.08340870824023282 7.807326493373652 >500 0.00338184180292255 1.2053715037595816 >1k 9.366484784683821E-4 1.161377810964535 >5k 2.077653398146186E-4 0.7572265739012242 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7081 0.38258345679092387 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6888 0.3721557478288213 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4801 0.2593960141298158 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 4143 0.2238445504144609 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3972 0.2146054922148778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002809538165955097 0.0 0.0 2 0.0030796860665277025 0.0 0.0 3 0.0033498339671003082 0.0 0.0 4 0.0036740114477874348 0.0 0.0 5 0.004376395989276209 0.0 0.0 6 0.004808632630192378 0.0 0.0 7 0.005943253812597321 0.0 0.0 8 0.006969815834773222 0.0 0.0 9 0.008050407437063643 0.0 0.0 10-11 0.016506036724986198 0.0 0.0 12-13 0.03049969797464716 0.0 0.0 14-15 0.04660051284877445 0.0 0.0 16-17 0.06578101378942944 0.0 0.0 18-19 0.08504255910025621 0.0 0.0 20-21 0.09301192216714807 0.0 0.0 22-23 0.09879308723940183 0.0 0.0 24-25 0.10533066643325889 0.0 0.0 26-27 0.11348913303055157 0.0 0.0 28-29 0.12388982720259688 0.0 0.0 30-31 0.18307923221805472 0.0 0.0 32-33 0.19499275463330665 0.0 0.0 34-35 0.20255689584933959 0.0 0.0 36-37 0.20958074126422732 0.0 0.0 38-39 0.21814442971237893 0.0 0.0 40-41 0.22641095546990067 0.0 0.0 42-43 0.23438031853679253 0.0 0.0 44-45 0.2426738590843715 0.0 0.0 46-47 0.2512645623225804 0.0 0.0 48-49 0.26107093111336593 0.0 0.0 50-51 0.2723090837771863 0.0 0.0 52-53 0.2842226061924382 0.0 0.0 54-55 0.29789208996141203 0.0 0.0 56-57 0.31315544634376424 0.0 0.0 58-59 0.33006670491960943 0.0 0.0 60-61 0.3475993036667715 0.0 0.0 62-63 0.36796845536994593 0.0 0.0 64-65 0.3897153613660407 0.0 0.0 66-67 0.41416374636786146 0.0 0.0 68-69 0.4414756991157519 0.0 0.0 70-71 0.4693009328747303 0.0 0.0 72-73 0.499962719589721 0.0 0.0 74-75 0.5368919375979961 0.0 0.0 76-77 0.5828170806953391 0.0 0.0 78-79 0.6347935367655084 0.0 0.0 80-81 0.6885259541893997 0.0 0.0 82-83 0.7478504331551439 0.0 0.0 84-85 0.8167381478011582 0.0 0.0 86-87 0.8914340423094836 0.0 0.0 88-89 0.9740452703045863 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1115 0.0 73.19438 9 AGAGCGG 1490 0.0 56.046772 8 TCGGGAG 1980 0.0 55.86932 3 CGGGAGA 1955 0.0 55.855213 4 GGAGAGC 1750 0.0 55.31909 6 ATCGGGA 2280 0.0 55.190487 2 AAGCGGC 430 0.0 50.75906 9 ATTGGGA 1080 0.0 48.363983 2 CGGAAAG 680 0.0 48.173817 5 GATCGGG 2760 0.0 46.633247 1 GATTGGG 1215 0.0 46.516403 1 AGAGCGT 4110 0.0 43.17715 8 AAGGGGC 1340 0.0 41.429073 9 GAGCGTC 4355 0.0 40.530216 9 TGTGTTT 40785 0.0 39.88371 1 TTGTGTT 23305 0.0 38.618515 1 GAGGGGC 2355 0.0 38.4828 9 CGGAAGA 4700 0.0 37.47647 4 AAGAGCG 4555 0.0 36.87659 7 ATCGGAA 5145 0.0 36.08648 2 >>END_MODULE