FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850838
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70810.38258345679092387No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68880.3721557478288213No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA48010.2593960141298158No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT41430.2238445504144609Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39720.2146054922148778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11150.073.194389
AGAGCGG14900.056.0467728
TCGGGAG19800.055.869323
CGGGAGA19550.055.8552134
GGAGAGC17500.055.319096
ATCGGGA22800.055.1904872
AAGCGGC4300.050.759069
ATTGGGA10800.048.3639832
CGGAAAG6800.048.1738175
GATCGGG27600.046.6332471
GATTGGG12150.046.5164031
AGAGCGT41100.043.177158
AAGGGGC13400.041.4290739
GAGCGTC43550.040.5302169
TGTGTTT407850.039.883711
TTGTGTT233050.038.6185151
GAGGGGC23550.038.48289
CGGAAGA47000.037.476474
AAGAGCG45550.036.876597
ATCGGAA51450.036.086482
Produced by FastQC (version 0.11.2)