##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7740804 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.840594594566664 33.0 33.0 33.0 27.0 33.0 2 31.677584783182727 33.0 33.0 33.0 27.0 33.0 3 31.63535041062918 33.0 33.0 33.0 27.0 33.0 4 35.28151119702811 37.0 37.0 37.0 33.0 37.0 5 35.26436698306791 37.0 37.0 37.0 33.0 37.0 6 35.48814567582386 37.0 37.0 37.0 33.0 37.0 7 35.2381753884997 37.0 37.0 37.0 33.0 37.0 8 35.307960645948405 37.0 37.0 37.0 33.0 37.0 9 35.11196537207246 37.0 37.0 37.0 33.0 37.0 10-11 35.08465528903716 37.0 37.0 37.0 33.0 37.0 12-13 35.08476354652566 37.0 37.0 37.0 33.0 37.0 14-15 36.79742207656982 40.0 37.0 40.0 33.0 40.0 16-17 36.72864659795029 40.0 37.0 40.0 33.0 40.0 18-19 36.710293724011095 40.0 37.0 40.0 33.0 40.0 20-21 36.659217634240576 40.0 37.0 40.0 33.0 40.0 22-23 36.90570075149816 40.0 37.0 40.0 33.0 40.0 24-25 36.5650050046481 40.0 37.0 40.0 33.0 40.0 26-27 36.459153274001 40.0 37.0 40.0 33.0 40.0 28-29 36.317504551206824 40.0 37.0 40.0 33.0 40.0 30-31 36.28892761010355 40.0 37.0 40.0 33.0 40.0 32-33 36.212253662539446 40.0 37.0 40.0 33.0 40.0 34-35 36.18700357223875 40.0 37.0 40.0 33.0 40.0 36-37 36.07440609528416 40.0 37.0 40.0 33.0 40.0 38-39 35.96570272286961 40.0 37.0 40.0 27.0 40.0 40-41 35.8887204094045 37.0 37.0 40.0 27.0 40.0 42-43 35.785753585803235 37.0 37.0 40.0 27.0 40.0 44-45 35.630444008141794 37.0 37.0 40.0 27.0 40.0 46-47 35.5139987396658 37.0 37.0 40.0 27.0 40.0 48-49 35.394573160617426 37.0 37.0 40.0 27.0 40.0 50-51 34.26893989306537 37.0 35.0 38.5 24.5 38.5 52-53 34.69282073283344 37.0 35.0 38.5 27.0 40.0 54-55 35.41282765976247 37.0 37.0 40.0 27.0 40.0 56-57 35.512302598024704 37.0 37.0 40.0 27.0 40.0 58-59 35.205173390257656 37.0 37.0 40.0 27.0 40.0 60-61 35.020861528078996 37.0 37.0 40.0 27.0 40.0 62-63 34.74055866806601 37.0 33.0 40.0 27.0 40.0 64-65 34.50548986901102 37.0 33.0 40.0 27.0 40.0 66-67 34.263682041297 37.0 33.0 37.0 27.0 40.0 68-69 34.13836308218113 37.0 33.0 37.0 27.0 40.0 70-71 33.78342353068234 37.0 33.0 37.0 27.0 40.0 72-73 33.71386822350753 37.0 33.0 37.0 27.0 38.5 74-75 33.53594781627335 37.0 33.0 37.0 27.0 37.0 76-77 33.25646295914481 37.0 33.0 37.0 27.0 37.0 78-79 32.95027822691286 37.0 33.0 37.0 22.0 37.0 80-81 32.66737608909875 37.0 33.0 37.0 22.0 37.0 82-83 32.40936736028971 37.0 33.0 37.0 22.0 37.0 84-85 32.219396008476636 37.0 33.0 37.0 22.0 37.0 86-87 32.05704200752273 33.0 33.0 37.0 22.0 37.0 88-89 31.89175975777193 33.0 33.0 37.0 22.0 37.0 90-91 31.762160687701176 33.0 33.0 37.0 22.0 37.0 92-93 31.569399896961606 33.0 33.0 37.0 15.0 37.0 94-95 31.430936760574227 33.0 33.0 37.0 15.0 37.0 96-97 31.297471360856058 33.0 33.0 37.0 15.0 37.0 98-99 31.141881256779012 33.0 33.0 37.0 15.0 37.0 100-101 29.842893645156238 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13745649296393836 2306 2 0.17282475847838086 2306 3 0.17799372369583466 2306 4 0.3113322655461772 2306 5 0.28945565197152945 2306 6 0.1895840720847275 2306 7 0.3311021425501295 2306 8 0.1637999473901246 2306 9 0.260932845418715 2306 10-11 0.22840375373092314 2306 12-13 0.19291577695991435 2306 14-15 0.39883168662734647 2306 16-17 0.39711226764401886 2306 18-19 0.39361891338327837 2306 20-21 0.4380470754030412 2306 22-23 0.3524778076413497 2306 24-25 0.4411473752138093 2306 26-27 0.36879752343225647 2306 28-29 0.42078534459010086 2306 30-31 0.43630140297799613 2306 32-33 0.4294948702572654 2306 34-35 0.35947531707535063 2306 36-37 0.32410566783525496 2306 38-39 0.39599231081216857 2306 40-41 0.33400825510763354 2306 42-43 0.3231746282373038 2306 44-45 0.48450087776569006 2306 46-47 0.3649840738067809 2306 48-49 0.40260937715545 2306 50-51 0.44528464174477733 2306 52-53 0.4553249858694315 2306 54-55 0.3131027723470723 2306 56-57 0.33519076312504836 2306 58-59 0.48689034419641075 2306 60-61 0.36153383923171134 2306 62-63 0.42825663458442875 2306 64-65 0.3268267047009559 2306 66-67 0.31596475291897264 2306 68-69 0.2922356725582631 2306 70-71 0.3845248723961916 2306 72-73 0.19408947400157928 2306 74-75 0.1689360772072348 2306 76-77 0.16021577391405373 2306 78-79 0.1408789287731338 2306 80-81 0.33070476194333054 2306 82-83 0.3335797964925433 2306 84-85 0.3020670443139082 2306 86-87 0.3551882763473486 2306 88-89 0.3803586856327108 2306 90-91 0.3228509388779912 2306 92-93 0.37847146370247486 2306 94-95 0.19566569493280284 2306 96-97 0.15987292838402212 2306 98-99 0.2383979755445793 2306 100-101 0.16574148144926326 2307 1 0.26906211285345094 2307 2 0.247481871468203 2307 3 0.1954714718157149 2307 4 0.30575486689329523 2307 5 0.34570542784578606 2307 6 0.20869968037044373 2307 7 0.39630104780160735 2307 8 0.3069446767984658 2307 9 0.3304143284037693 2307 10-11 0.27987110918251545 2307 12-13 0.24210905961545137 2307 14-15 0.48114408915378704 2307 16-17 0.47394192869823826 2307 18-19 0.5146382203357476 2307 20-21 0.4265569183289557 2307 22-23 0.3593422124141341 2307 24-25 0.4087981653204693 2307 26-27 0.3087833823666699 2307 28-29 0.4244204103731235 2307 30-31 0.4755738485365839 2307 32-33 0.46759712572339396 2307 34-35 0.4261371460090544 2307 36-37 0.2839027875234805 2307 38-39 0.26848167964025293 2307 40-41 0.2716489976150669 2307 42-43 0.2243240126949928 2307 44-45 0.35095377076383727 2307 46-47 0.3265674671888945 2307 48-49 0.26731111231156035 2307 50-51 0.08132609062889173 2307 52-53 0.2328092632373 2307 54-55 0.3282234957742176 2307 56-57 0.2865056115822142 2307 58-59 0.26923221291454524 2307 60-61 0.22158989603955348 2307 62-63 0.2840413453184354 2307 64-65 0.35897134276748943 2307 66-67 0.3485303752442661 2307 68-69 0.16959738274761094 2307 70-71 0.22821437113262988 2307 72-73 0.19294868244021046 2307 74-75 0.1729993669333041 2307 76-77 0.19008992519639634 2307 78-79 0.23330855329062672 2307 80-81 0.32378494313841344 2307 82-83 0.4069555191923371 2307 84-85 0.4441494684579155 2307 86-87 0.33908951893427997 2307 88-89 0.3976250620354058 2307 90-91 0.35961077419208465 2307 92-93 0.3651511212045122 2307 94-95 0.34048306205583856 2307 96-97 0.29274985713402657 2307 98-99 0.24024027221601685 2307 100-101 0.2263715707950844 2201 1 0.20619428418927654 2201 2 0.0979812481294573 2201 3 0.1587321214993409 2201 4 0.25051388178285805 2201 5 0.22991167064211027 2201 6 0.2342085835294796 2201 7 0.32605100663773356 2201 8 0.2107063496985191 2201 9 0.325591333084688 2201 10-11 0.27579376813388734 2201 12-13 0.3113316503524075 2201 14-15 0.3905254548537016 2201 16-17 0.3353676102544725 2201 18-19 0.31871320916268786 2201 20-21 0.43442180671603836 2201 22-23 0.3488343677548471 2201 24-25 0.3937486510542456 2201 26-27 0.27425888397793585 2201 28-29 0.45673287950021546 2201 30-31 0.4623000264115902 2201 32-33 0.3266188367294376 2201 34-35 0.30917245285637307 2201 36-37 0.34302329183979907 2201 38-39 0.3266874049478119 2201 40-41 0.3587026047470019 2201 42-43 0.4066752934796867 2201 44-45 0.47700086224173077 2201 46-47 0.408618369265902 2201 48-49 0.3518786563569094 2201 50-51 0.2590952435938618 2201 52-53 0.37155129508637685 2201 54-55 0.524292227691511 2201 56-57 0.49889570402452676 2201 58-59 0.5151151193196455 2201 60-61 0.5061781396356295 2201 62-63 0.4973509212128775 2201 64-65 0.5851555687689398 2201 66-67 0.5023840200117462 2201 68-69 0.44082900601578956 2201 70-71 0.535603640297353 2201 72-73 0.32501205129209154 2201 74-75 0.40066784481066264 2201 76-77 0.4202985672588184 2201 78-79 0.4228329340682748 2201 80-81 0.44369649960349733 2201 82-83 0.5631630321883634 2201 84-85 0.499122770741927 2201 86-87 0.44617511628097617 2201 88-89 0.4528853353635576 2201 90-91 0.3870835472262115 2201 92-93 0.6006073345980667 2201 94-95 0.5785137820708961 2201 96-97 0.4449858790567305 2201 98-99 0.45955742226423624 2201 100-101 0.3211924491146583 1101 1 0.12334870511970664 1101 2 0.1645754688372989 1101 3 0.11880654844695471 1101 4 0.24119735431348488 1101 5 0.27773034223213244 1101 6 0.16378428941372647 1101 7 0.3213530660382773 1101 8 0.17529808760514243 1101 9 0.22267993609082026 1101 10-11 0.20361447209281636 1101 12-13 0.18433241448885695 1101 14-15 0.3637424008140897 1101 16-17 0.3705684108708027 1101 18-19 0.3459007816835751 1101 20-21 0.3141066097061369 1101 22-23 0.31951122366874074 1101 24-25 0.3854005958914186 1101 26-27 0.3578098834495833 1101 28-29 0.40601088901181015 1101 30-31 0.4589425501342177 1101 32-33 0.34862301221992453 1101 34-35 0.3092343658921024 1101 36-37 0.37749607907675653 1101 38-39 0.2841713607940264 1101 40-41 0.3608551174771435 1101 42-43 0.336020359747188 1101 44-45 0.41874019608715685 1101 46-47 0.35697808771430317 1101 48-49 0.4077464935767168 1101 50-51 0.42028170744609383 1101 52-53 0.4285005694992776 1101 54-55 0.4564974535627684 1101 56-57 0.47247640691017523 1101 58-59 0.4949037814985431 1101 60-61 0.4630207004090252 1101 62-63 0.5461415760838051 1101 64-65 0.4553179813050008 1101 66-67 0.4494018469127212 1101 68-69 0.44306087247545634 1101 70-71 0.5070753180093206 1101 72-73 0.4136858906224816 1101 74-75 0.457738005210814 1101 76-77 0.5558993620520738 1101 78-79 0.6034412803108609 1101 80-81 0.6194625984913742 1101 82-83 0.48805639704625037 1101 84-85 0.40405624634107795 1101 86-87 0.4281214862902871 1101 88-89 0.5940759438782202 1101 90-91 0.4365556593855757 1101 92-93 0.6071820337175495 1101 94-95 0.5860487326789077 1101 96-97 0.45805506723097267 1101 98-99 0.44682074905231417 1101 100-101 0.5259984164009417 2304 1 0.18294373037879197 2304 2 0.1283989891464543 2304 3 0.13285984118789074 2304 4 0.23791958221590903 2304 5 0.2889302097750246 2304 6 0.21779375731877337 2304 7 0.3613719050182169 2304 8 0.245756475205404 2304 9 0.24819897072281805 2304 10-11 0.23554829992043125 2304 12-13 0.2516625651608635 2304 14-15 0.3845656327777647 2304 16-17 0.3411268869358324 2304 18-19 0.3340915634328141 2304 20-21 0.37341967332935866 2304 22-23 0.2470191730870397 2304 24-25 0.33910572295596353 2304 26-27 0.32950317721601863 2304 28-29 0.26911320344648004 2304 30-31 0.2399774050049217 2304 32-33 0.26042787924240685 2304 34-35 0.256519081978027 2304 36-37 0.3060274098100422 2304 38-39 0.3113768794180416 2304 40-41 0.40074947149287254 2304 42-43 0.3623226154709229 2304 44-45 0.3895702664248617 2304 46-47 0.40878403130828644 2304 48-49 0.32194338706528924 2304 50-51 0.273765509181672 2304 52-53 0.20879336630642342 2304 54-55 0.3954760987353225 2304 56-57 0.4350414161504048 2304 58-59 0.475949381656001 2304 60-61 0.5162537907034448 2304 62-63 0.518028428009977 2304 64-65 0.5230699508741878 2304 66-67 0.489652138552259 2304 68-69 0.4750057060506734 2304 70-71 0.5324806180709416 2304 72-73 0.42865202203018526 2304 74-75 0.4499428971966779 2304 76-77 0.5231329277130783 2304 78-79 0.6234297863268381 2304 80-81 0.6482071853744245 2304 82-83 0.6283518454469643 2304 84-85 0.600532537917907 2304 86-87 0.605139948061634 2304 88-89 0.5583155376246616 2304 90-91 0.5548205121143752 2304 92-93 0.545196769468788 2304 94-95 0.5731398078704792 2304 96-97 0.5604795083049723 2304 98-99 0.6325858691365092 2304 100-101 0.6071459639986294 2202 1 0.1340810526296714 2202 2 0.1192393346819749 2202 3 0.1263174036325232 2202 4 0.1957710817638798 2202 5 0.2791274383551752 2202 6 0.19154811506354008 2202 7 0.27753539641459923 2202 8 0.20056745566396472 2202 9 0.2086849216258173 2202 10-11 0.21008244075306237 2202 12-13 0.20093055474763588 2202 14-15 0.2968166025932959 2202 16-17 0.28191930861736836 2202 18-19 0.24310789987338666 2202 20-21 0.32787281226621445 2202 22-23 0.27114906033747843 2202 24-25 0.34209045686518635 2202 26-27 0.3175783012586777 2202 28-29 0.3895893881634578 2202 30-31 0.42212176314275496 2202 32-33 0.35722680768316906 2202 34-35 0.3011880445550901 2202 36-37 0.267232218522814 2202 38-39 0.2577455514283997 2202 40-41 0.2792002157488085 2202 42-43 0.30847313567906554 2202 44-45 0.25020573370512267 2202 46-47 0.28820005571468243 2202 48-49 0.31809904737134076 2202 50-51 0.3118329230774677 2202 52-53 0.22730845249377296 2202 54-55 0.22771222273866698 2202 56-57 0.3794183002230582 2202 58-59 0.3700796922086269 2202 60-61 0.35800136351898004 2202 62-63 0.38970437204942954 2202 64-65 0.3880289963882433 2202 66-67 0.4088782212231763 2202 68-69 0.3462588014478669 2202 70-71 0.42781552568524006 2202 72-73 0.44368393599099676 2202 74-75 0.37755036303770595 2202 76-77 0.5358957908080839 2202 78-79 0.4589849017825145 2202 80-81 0.40911553138163015 2202 82-83 0.4914938663520445 2202 84-85 0.4179422006972473 2202 86-87 0.3660895660314125 2202 88-89 0.356671008654164 2202 90-91 0.3257348145879746 2202 92-93 0.49520750320264284 2202 94-95 0.5340906352750672 2202 96-97 0.40802904221903447 2202 98-99 0.42660491168745196 2202 100-101 0.40878792292017607 1102 1 0.14256397553937106 1102 2 0.06364175343453482 1102 3 0.10882126884672871 1102 4 0.10869910729483934 1102 5 0.20054008734837936 1102 6 0.16018255720436514 1102 7 0.15245267161926535 1102 8 0.1836186153699373 1102 9 0.15152878397742597 1102 10-11 0.20928658216534046 1102 12-13 0.1896140861717157 1102 14-15 0.15081920727293152 1102 16-17 0.12761598956394948 1102 18-19 0.22014590580548088 1102 20-21 0.07756508311320687 1102 22-23 0.2238952401170593 1102 24-25 0.1498176258845163 1102 26-27 0.23648844547970072 1102 28-29 0.10372480599630762 1102 30-31 0.14546609810625455 1102 32-33 0.1843442435900542 1102 34-35 0.1834566666682349 1102 36-37 0.2748104474823734 1102 38-39 0.12750009084083302 1102 40-41 0.1638185763311384 1102 42-43 0.17724546428816979 1102 44-45 0.18750381080533174 1102 46-47 0.19565754544405678 1102 48-49 0.18304569962109696 1102 50-51 0.19349515649814464 1102 52-53 0.277814437730143 1102 54-55 0.3985940867709914 1102 56-57 0.45801273686542743 1102 58-59 0.4738376298918823 1102 60-61 0.42064730989772414 1102 62-63 0.3570211693988341 1102 64-65 0.39963455889559896 1102 66-67 0.4149790951654708 1102 68-69 0.3379857550638832 1102 70-71 0.39199933840905743 1102 72-73 0.37419751702870485 1102 74-75 0.3882136777263625 1102 76-77 0.3769690945423534 1102 78-79 0.3902720925122338 1102 80-81 0.39628557104701656 1102 82-83 0.3879092433149438 1102 84-85 0.32269269421176006 1102 86-87 0.286649104501457 1102 88-89 0.2651756136087293 1102 90-91 0.341462374734391 1102 92-93 0.3392110006723641 1102 94-95 0.3698060648133783 1102 96-97 0.189184085845298 1102 98-99 0.2851815775558002 1102 100-101 0.36462499865003295 2305 1 0.12175598319437952 2305 2 0.02859878670276572 2305 3 0.026723251806991755 2305 4 -0.0012608402904774607 2305 5 0.08753938665453376 2305 6 0.13302032754823045 2305 7 0.10701481078427832 2305 8 0.15795773066866303 2305 9 0.056090112230812395 2305 10-11 0.10093207410901783 2305 12-13 0.07276701649190187 2305 14-15 0.0955959599922167 2305 16-17 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-0.32796187710733093 1213 96-97 -0.26390115349488497 1213 98-99 -0.2737437821547779 1213 100-101 -0.06491697541058628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1213.0 3 509.0 4 361.0 5 432.0 6 1033.0 7 4485.0 8 18940.0 9 56977.0 10 71909.0 11 55718.0 12 35471.0 13 25577.0 14 21633.0 15 21540.0 16 22042.0 17 22158.0 18 22801.0 19 24922.0 20 26925.0 21 29694.0 22 32871.0 23 35527.0 24 39027.0 25 44840.0 26 52712.0 27 64251.0 28 78313.0 29 98023.0 30 120394.0 31 155404.0 32 213622.0 33 306271.0 34 469430.0 35 801679.0 36 1568692.0 37 2513459.0 38 681371.0 39 578.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.438340627478624 26.25665724008118 22.31052938637253 14.994472746067675 2 32.29485336608665 24.697818045115895 29.73688952591233 13.270439062885123 3 36.419391986631354 20.245438631412117 26.23945577033931 17.09571361161722 4 30.799388870923895 19.112897517608392 31.07969458038965 19.008019031078064 5 31.18620694296229 17.83960262627774 32.64985439620951 18.324336034550456 6 27.59273651900162 18.125689495656566 36.47031003243346 17.81126395290835 7 33.37192487777117 19.108324494916832 28.199627493080165 19.320123134231835 8 29.72783406324882 27.95269149142422 23.63190478906561 18.687569656261346 9 29.433381242788688 27.230123934355166 24.124057469620343 19.212437353235803 10-11 25.047302487788247 28.620400460993178 28.662443525038412 17.669853526180162 12-13 25.640086115886024 25.788811983742978 28.515653368490874 20.055448531880124 14-15 24.4597080410353 26.504430884882353 28.986719696657143 20.049141377425205 16-17 24.375933786072608 27.508941650276174 29.726414017062492 18.388710546588722 18-19 23.716929213940972 27.35842859317171 30.12428187386067 18.80036031902665 20-21 23.05679112479936 28.72180785606508 29.456086440820872 18.765314578314687 22-23 24.512721766121317 27.6080876391278 28.797676035026015 19.081514559724866 24-25 22.943785273524313 28.59849697226294 29.153572912631365 19.304144841581387 26-27 22.196496754319643 29.273000218617938 29.157465362518113 19.3730376645443 28-29 22.45120957934551 29.274588586304585 28.593221121389295 19.680980712960615 30-31 22.80949847476559 28.944601979886396 28.82168249059889 19.42421705474913 32-33 22.768922428016385 28.526938684680548 29.327726131819336 19.376412755483734 34-35 21.80929834412385 28.41218095701971 28.960903328743115 20.81761737011332 36-37 20.464557246021357 29.058687640473874 29.482331152035634 20.994423961469135 38-39 20.558152360719216 29.462581084081467 29.438034963980254 20.541231591219066 40-41 21.496255390394996 28.176943670230713 29.98023756478329 20.346563374591 42-43 22.291298689300852 28.20312758452746 29.996700221001678 19.50887350517001 44-45 21.097523733428545 29.002228320158846 30.044754113874113 19.8554938325385 46-47 22.175527134091016 29.24881656178399 28.980195719274516 19.59546058485048 48-49 21.425664899315624 28.911201194098023 29.231527906111726 20.431606000474627 50-51 20.736287099287175 28.60899351313938 29.17369853337109 21.48102085420236 52-53 22.939218000697146 28.328481263999933 28.076926686877602 20.655374048425323 54-55 23.667085915454127 28.05810537085008 28.290051300053083 19.984757413642708 56-57 23.894869927512126 27.60426523535971 28.161911076465273 20.338953760662896 58-59 22.937053206364737 27.63703990631067 28.50815137985634 20.917755507468254 60-61 21.241213881266837 27.790545723730293 28.190440508694635 22.77779988630823 62-63 22.147314653262367 27.95557461187892 28.50406934241515 21.393041392443564 64-65 21.385577053852113 28.74705163538071 29.35754605996773 20.50982525079944 66-67 21.443543310913242 28.301212696122946 28.66417187371487 21.59107211924894 68-69 21.111210903235776 27.428048080708418 30.75295056122717 20.707790454828633 70-71 20.903217198993005 29.611024917208884 29.38395957468243 20.101798309115683 72-73 21.02167946893373 31.216953538171765 27.946925725067285 19.814441267827224 74-75 21.826586418076936 30.807698600799625 27.519321842612072 19.846393138511374 76-77 21.915963693971936 29.974485669347423 27.699160211516855 20.410390425163786 78-79 21.62288316324101 29.91039354166796 28.29829514279266 20.168428152298368 80-81 21.062673750174792 30.627266804237863 28.05697908951042 20.25308035607693 82-83 20.41964151241243 30.51979929997183 28.357250865520978 20.703308322094767 84-85 20.610790775099524 30.035635718303077 28.53786117085275 20.815712335744646 86-87 20.476937883772603 30.233847651458913 28.997548903802727 20.29166556096576 88-89 20.5209869081069 30.594823194164555 28.856925018475092 20.027264879253455 90-91 21.05998849726627 29.588540082794573 28.648362776056064 20.70310864388309 92-93 20.740812394754425 30.022073869656435 28.125914554745496 21.111199180843645 94-95 20.46623675829191 30.61067375591452 27.695275360364562 21.22781412542901 96-97 20.766178961285313 30.885125700922394 27.435707136564947 20.912988201227343 98-99 21.059195515186307 29.98841841649756 27.760910036110335 21.1914760322058 100-101 21.34564145795042 30.126423312459334 27.27062659017844 21.257308639411804 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2547.0 1 1853.0 2 1130.5 3 1369.5 4 1489.0 5 1322.5 6 1504.5 7 1885.0 8 2189.5 9 2363.0 10 2469.5 11 2584.0 12 2704.0 13 2720.0 14 2544.5 15 2429.5 16 2433.0 17 2408.0 18 2501.0 19 2833.0 20 3327.0 21 4077.5 22 5455.0 23 7409.0 24 9979.0 25 13325.0 26 17754.5 27 23150.0 28 29560.0 29 36996.0 30 45373.0 31 55994.5 32 71343.5 33 93670.5 34 125558.0 35 176835.5 36 247366.0 37 329685.5 38 411219.0 39 451719.5 40 468603.0 41 456545.0 42 431771.5 43 447297.0 44 448204.5 45 439183.5 46 456238.5 47 427186.5 48 343390.0 49 276268.0 50 233005.0 51 206925.0 52 188586.0 53 161916.5 54 128063.0 55 95869.0 56 73588.5 57 60842.0 58 50930.0 59 39249.0 60 28549.0 61 21247.5 62 15367.0 63 11038.0 64 8028.5 65 5652.0 66 4090.0 67 3091.0 68 2272.5 69 1543.0 70 1068.0 71 755.5 72 556.5 73 435.0 74 324.0 75 243.5 76 199.5 77 162.0 78 127.0 79 106.0 80 97.5 81 79.0 82 55.5 83 46.5 84 36.0 85 32.0 86 32.5 87 24.0 88 16.5 89 15.5 90 13.5 91 8.0 92 6.0 93 4.5 94 3.5 95 3.0 96 2.5 97 2.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2229484172445136 2 0.2065418527584473 3 0.18171239059921943 4 0.16552544154328155 5 0.17659664293269794 6 0.13585152136651438 7 0.1214860885251713 8 0.11739090668101144 9 0.10544124357108126 10-11 0.0912954261598666 12-13 0.11793994525633254 14-15 0.11403208245551755 16-17 0.1055575105635022 18-19 0.09891737344079503 20-21 0.10303839239438177 22-23 0.08981625164517794 24-25 0.40691509564122796 26-27 0.1937395650374302 28-29 0.09256144452178353 30-31 0.1782114622718777 32-33 0.20792413811278518 34-35 0.20723299543561627 36-37 0.2131949084358679 38-39 0.2140475330469548 40-41 0.21057890110639668 42-43 0.22684336149061518 44-45 0.2086475771767377 46-47 0.22801894996953803 48-49 0.21722549750646056 50-51 0.2200934166528438 52-53 0.21233582454742425 54-55 0.19482472363335904 56-57 0.20217538126530527 58-59 0.19728570830626896 60-61 0.1779078762361119 62-63 0.1658161090243339 64-65 0.17071870053808363 66-67 0.18315280944976775 68-69 0.17607990074416044 70-71 0.16887780649141873 72-73 0.17222371216219917 74-75 0.16236685491584596 76-77 0.17287609917522778 78-79 0.17098999018706584 80-81 0.17638348677992624 82-83 0.20079955518832412 84-85 0.1990620095793667 86-87 0.20267920489912936 88-89 0.20946790540104102 90-91 0.1913819288022278 92-93 0.17235289770933354 94-95 0.17337992280905187 96-97 0.1822872662839674 98-99 0.19605844560849234 100-101 0.20723945471297295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7740804.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.039050854013475 #Duplication Level Percentage of deduplicated Percentage of total 1 89.31753623921784 40.227770568333234 2 5.296841448944472 4.77129422769313 3 1.6805796216113678 2.2707513312591936 4 0.7382517390947142 1.3300063048060287 5 0.4530620999388474 1.020274347958594 6 0.431156159941879 1.1651318528186072 7 0.2535639965730317 0.7994197215479771 8 0.20966628121810887 0.7554536241723437 9 0.1508295136700956 0.61138963228262 >10 1.1250052210136257 10.40723956032042 >50 0.15937712170378107 4.983137014715529 >100 0.15150056595530367 14.106028478411162 >500 0.019952834119005804 6.3713592034249436 >1k 0.012241051805080453 9.530592954819852 >5k 2.90857649738609E-4 0.8327783482373714 >10k+ 1.4524754303550284E-4 0.8173728291989707 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 16496 0.21310447855287384 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 14324 0.18504537771528642 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10942 0.14135482567443897 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10682 0.1379960014489451 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 10029 0.12956018522107007 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9804 0.1266535104105465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9543 0.12328176763033917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027258150445354256 0.0 0.0 2 0.002855000591669806 0.0 0.0 3 0.003177964459505757 0.0 0.0 4 0.003371742780207327 0.0 0.0 5 0.003927240632885163 0.0 0.0 6 0.004637761142124255 0.0 0.0 7 0.005335363096649909 0.0 0.0 8 0.006045883605889001 0.0 0.0 9 0.006678892786847464 0.0 0.0 10-11 0.011400624534609067 0.0 1.9377832070157052E-5 12-13 0.015250353839213601 0.0 2.5837109426876072E-5 14-15 0.020534042717009756 0.0 2.5837109426876072E-5 16-17 0.02689643091337799 0.0 4.5214941497033125E-5 18-19 0.033820776239780775 0.0 5.1674218853752144E-5 20-21 0.03896236101572911 0.0 5.1674218853752144E-5 22-23 0.043819737587981814 0.0 5.1674218853752144E-5 24-25 0.04963954648638565 0.0 6.459277356719018E-5 26-27 0.055911504799759815 0.0 8.397060563734724E-5 28-29 0.06314589543928512 0.0 9.042988299406625E-5 30-31 0.1112352153600582 0.0 1.0334843770750429E-4 32-33 0.12167340756851613 0.0 1.1626699242094231E-4 34-35 0.13060658815285853 0.0 1.1626699242094231E-4 36-37 0.13976584344468612 0.0 1.2918554713438037E-4 38-39 0.15006839082865292 0.0 1.2918554713438037E-4 40-41 0.16147547464061873 0.0 1.421041018478184E-4 42-43 0.17377393872781172 0.0 1.421041018478184E-4 44-45 0.18717047996564698 0.0 1.421041018478184E-4 46-47 0.20099333350902568 0.0 1.421041018478184E-4 48-49 0.21645038422365429 0.0 1.5502265656125642E-4 50-51 0.2333349352341178 0.0 1.5502265656125642E-4 52-53 0.25052953155770386 0.0 1.6148193391797545E-4 54-55 0.2703013795466207 0.0 1.6794121127469447E-4 56-57 0.29226938183682216 0.0 1.6794121127469447E-4 58-59 0.3161880858887527 0.0 1.6794121127469447E-4 60-61 0.3418184984402137 0.0 1.744004886314135E-4 62-63 0.3683015356027617 0.0 1.808597659881325E-4 64-65 0.3983113382020782 0.0 1.808597659881325E-4 66-67 0.43104049656857346 0.0 1.808597659881325E-4 68-69 0.4669799157813581 0.0 1.808597659881325E-4 70-71 0.505535342323614 0.0 1.808597659881325E-4 72-73 0.5482247063741699 0.0 1.808597659881325E-4 74-75 0.5952482455310844 0.0 1.808597659881325E-4 76-77 0.6477104962223562 0.0 1.808597659881325E-4 78-79 0.7066707799344873 0.0 1.808597659881325E-4 80-81 0.7740539613197802 0.0 1.808597659881325E-4 82-83 0.8472181959393366 0.0 1.808597659881325E-4 84-85 0.9296514935657847 0.0 1.808597659881325E-4 86-87 1.021689736621674 0.0 1.808597659881325E-4 88-89 1.1197157297872418 0.0 1.808597659881325E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4875 0.0 79.776794 2 GAGCGGC 3480 0.0 77.71472 9 CGGGAGA 4520 0.0 73.10661 4 AGAGCGG 3785 0.0 72.20497 8 AAGCGGC 1365 0.0 63.61011 9 CGGAAAG 1960 0.0 61.768906 5 TCGGAGA 1025 0.0 59.751892 4 TCGGGAA 1700 0.0 56.136356 3 GTTCGGA 765 0.0 55.866108 2 TCGGGAG 5970 0.0 55.749474 3 GATCGGG 7485 0.0 55.394375 1 GAGAGCG 5380 0.0 55.21057 7 CGGAGAG 2195 0.0 50.82989 5 AAGCGTC 690 0.0 48.822155 9 GAACCGG 430 0.0 48.599014 1 ATTCGGA 920 0.0 48.002346 2 GGTTCGG 1080 0.0 47.49449 1 TCGGAAA 1905 0.0 47.103443 4 GGTCCGG 575 0.0 47.081493 1 GGATCGG 1785 0.0 46.29715 1 >>END_MODULE