FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740804
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT164960.21310447855287384Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA143240.18504537771528642No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC109420.14135482567443897No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG106820.1379960014489451No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC100290.12956018522107007No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98040.1266535104105465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95430.12328176763033917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA48750.079.7767942
GAGCGGC34800.077.714729
CGGGAGA45200.073.106614
AGAGCGG37850.072.204978
AAGCGGC13650.063.610119
CGGAAAG19600.061.7689065
TCGGAGA10250.059.7518924
TCGGGAA17000.056.1363563
GTTCGGA7650.055.8661082
TCGGGAG59700.055.7494743
GATCGGG74850.055.3943751
GAGAGCG53800.055.210577
CGGAGAG21950.050.829895
AAGCGTC6900.048.8221559
GAACCGG4300.048.5990141
ATTCGGA9200.048.0023462
GGTTCGG10800.047.494491
TCGGAAA19050.047.1034434
GGTCCGG5750.047.0814931
GGATCGG17850.046.297151
Produced by FastQC (version 0.11.2)