FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6100707
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC136660.22400682412710526No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG131000.21472921089309813No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT100800.1652267515879717Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA100570.16484974610319755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC27450.079.502059
AGAGCGG29750.076.385598
CGGGAGA39500.075.353644
GATCGGG54000.069.65531
CGGAAAG20350.069.282865
GAGAGCG36850.068.365057
AAGCGGC12750.065.488559
TCGGGAG46950.064.511963
ATCGGGA58250.063.0931472
TCGGGAA15300.062.3677023
GTTCGGA8700.060.5816232
AGAGCGT76550.060.3638088
GATCCGG10850.059.0917431
GAGCGTC78000.058.9375429
AAGGGGC36600.056.7748379
AAGAGCG80650.055.0615737
TCGGAAA20550.053.8242344
CGGAAGA84750.053.6611024
TCGGAAG85700.053.5120353
GGTCCGG4800.051.4499361
Produced by FastQC (version 0.11.2)