##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6944751 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18009155403844 33.0 33.0 33.0 33.0 33.0 2 32.13013439934708 33.0 33.0 33.0 33.0 33.0 3 32.10873003222146 33.0 33.0 33.0 33.0 33.0 4 35.872463245982466 37.0 37.0 37.0 33.0 37.0 5 35.83092151180078 37.0 37.0 37.0 33.0 37.0 6 35.924757849489495 37.0 37.0 37.0 33.0 37.0 7 35.823179549561964 37.0 37.0 37.0 33.0 37.0 8 35.81360886805013 37.0 37.0 37.0 33.0 37.0 9 35.742074553860895 37.0 37.0 37.0 33.0 37.0 10-11 35.695366255752006 37.0 37.0 37.0 33.0 37.0 12-13 35.708791143123776 37.0 37.0 37.0 33.0 37.0 14-15 37.55073745624573 40.0 37.0 40.0 33.0 40.0 16-17 37.52254983656002 40.0 37.0 40.0 33.0 40.0 18-19 37.494613197795 40.0 37.0 40.0 33.0 40.0 20-21 37.454671017002624 40.0 37.0 40.0 33.0 40.0 22-23 37.537168935214524 40.0 37.0 40.0 33.0 40.0 24-25 37.33137192391779 40.0 37.0 40.0 33.0 40.0 26-27 37.25503866157332 40.0 37.0 40.0 33.0 40.0 28-29 37.184779267104034 40.0 37.0 40.0 33.0 40.0 30-31 37.15989198172836 40.0 37.0 40.0 33.0 40.0 32-33 37.079098084294166 40.0 37.0 40.0 33.0 40.0 34-35 37.08220388319178 40.0 37.0 40.0 33.0 40.0 36-37 36.98468181220608 40.0 37.0 40.0 33.0 40.0 38-39 36.93499450160272 40.0 37.0 40.0 33.0 40.0 40-41 36.82612335561059 40.0 37.0 40.0 33.0 40.0 42-43 36.76201472162213 40.0 37.0 40.0 33.0 40.0 44-45 36.663461368161364 40.0 37.0 40.0 33.0 40.0 46-47 36.56203080571211 40.0 37.0 40.0 33.0 40.0 48-49 36.45697851514043 40.0 37.0 40.0 33.0 40.0 50-51 35.33477427772428 37.0 35.0 38.5 30.0 38.5 52-53 35.73753249036575 37.0 37.0 38.5 33.0 40.0 54-55 36.52246336837707 38.5 37.0 40.0 33.0 40.0 56-57 36.588482366034434 38.5 37.0 40.0 33.0 40.0 58-59 36.39248253825083 37.0 37.0 40.0 33.0 40.0 60-61 36.21949347068023 37.0 37.0 40.0 33.0 40.0 62-63 36.00586270119692 37.0 37.0 40.0 33.0 40.0 64-65 35.78882475412006 37.0 37.0 40.0 33.0 40.0 66-67 35.54900283681877 37.0 37.0 40.0 30.0 40.0 68-69 35.35714462620762 37.0 37.0 40.0 27.0 40.0 70-71 35.045678455570254 37.0 33.0 37.0 27.0 40.0 72-73 34.85587769813489 37.0 33.0 37.0 27.0 40.0 74-75 34.61577304931451 37.0 33.0 37.0 27.0 40.0 76-77 34.34397813542919 37.0 33.0 37.0 27.0 37.0 78-79 34.056160472852085 37.0 33.0 37.0 27.0 37.0 80-81 33.7947404449778 37.0 33.0 37.0 27.0 37.0 82-83 33.54970574178974 37.0 33.0 37.0 27.0 37.0 84-85 33.37231932433575 37.0 33.0 37.0 27.0 37.0 86-87 33.201975204006594 37.0 33.0 37.0 27.0 37.0 88-89 33.01868144732619 37.0 33.0 37.0 27.0 37.0 90-91 32.87436777790881 37.0 33.0 37.0 27.0 37.0 92-93 32.70242424818399 35.0 33.0 37.0 27.0 37.0 94-95 32.57608904912502 33.0 33.0 37.0 27.0 37.0 96-97 32.44661680454778 33.0 33.0 37.0 24.5 37.0 98-99 32.30581895592801 33.0 33.0 37.0 22.0 37.0 100-101 31.004260771912485 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.28489856084288334 2306 2 0.3483004044375946 2306 3 0.3360443057882563 2306 4 0.40905404907349663 2306 5 0.4642387748586927 2306 6 0.4184420894302079 2306 7 0.4180557211576712 2306 8 0.4001140889863848 2306 9 0.416499755708422 2306 10-11 0.4203434475972614 2306 12-13 0.38951592929675627 2306 14-15 0.5971962976896137 2306 16-17 0.6287182021923456 2306 18-19 0.6563356233929696 2306 20-21 0.585253060878614 2306 22-23 0.5280209473797157 2306 24-25 0.5866183744738649 2306 26-27 0.5953164405662434 2306 28-29 0.6068869482392429 2306 30-31 0.6356412077871596 2306 32-33 0.5559447587947091 2306 34-35 0.5560900798958457 2306 36-37 0.5214375615314282 2306 38-39 0.5238058328434718 2306 40-41 0.524013805335251 2306 42-43 0.44210918219822304 2306 44-45 0.6259404230736152 2306 46-47 0.589796552792258 2306 48-49 0.647323168079339 2306 50-51 0.5848699285891215 2306 52-53 0.6077082634790614 2306 54-55 0.5419975289209802 2306 56-57 0.5511846286966389 2306 58-59 0.7131657825046673 2306 60-61 0.7344422050484809 2306 62-63 0.6554510549209667 2306 64-65 0.6190661428970827 2306 66-67 0.6549862200445631 2306 68-69 0.6878166923709372 2306 70-71 0.7167309316762598 2306 72-73 0.5212763789147132 2306 74-75 0.5054551678713395 2306 76-77 0.5897231482503642 2306 78-79 0.5402285974571086 2306 80-81 0.5982538299228466 2306 82-83 0.6018065216948472 2306 84-85 0.5736955037453413 2306 86-87 0.5431354002085342 2306 88-89 0.6151726517428884 2306 90-91 0.5520124833923603 2306 92-93 0.5975030324132504 2306 94-95 0.4514682010882325 2306 96-97 0.39481115934697897 2306 98-99 0.4509425879060416 2306 100-101 0.4561951049489217 2307 1 0.23096533816228515 2307 2 0.19779850704161106 2307 3 0.25992360247762747 2307 4 0.29649103211057337 2307 5 0.34754194843426944 2307 6 0.19621044527288234 2307 7 0.31415133820737395 2307 8 0.22762917173488972 2307 9 0.23998915646187413 2307 10-11 0.253660653638633 2307 12-13 0.26665876430251245 2307 14-15 0.4778226708051321 2307 16-17 0.37708402254923357 2307 18-19 0.4103336709001866 2307 20-21 0.40300507707707567 2307 22-23 0.321657955899056 2307 24-25 0.40830197766672427 2307 26-27 0.344906959440344 2307 28-29 0.31663860803029564 2307 30-31 0.4624900497213318 2307 32-33 0.4586663254032928 2307 34-35 0.40464439156466625 2307 36-37 0.349734416192959 2307 38-39 0.30188638952775193 2307 40-41 0.3858771790499418 2307 42-43 0.3798813517723971 2307 44-45 0.37114515072325105 2307 46-47 0.42025587069390014 2307 48-49 0.2899449750477032 2307 50-51 0.20386665893850164 2307 52-53 0.28114807711414613 2307 54-55 0.3853552655445114 2307 56-57 0.24560013775661815 2307 58-59 0.2775341665844522 2307 60-61 0.3762280902818489 2307 62-63 0.4200731855830284 2307 64-65 0.4064720909407953 2307 66-67 0.3612905850883976 2307 68-69 0.30583244144634847 2307 70-71 0.2663255397658446 2307 72-73 0.23742697223835307 2307 74-75 0.16868446869296605 2307 76-77 0.16170832032455706 2307 78-79 0.23158253240156768 2307 80-81 0.2944955395764879 2307 82-83 0.325205400130649 2307 84-85 0.3765579545886695 2307 86-87 0.4157918923331323 2307 88-89 0.3455365402682915 2307 90-91 0.35584166782200555 2307 92-93 0.42027936007391276 2307 94-95 0.2727830861769718 2307 96-97 0.2656771777079854 2307 98-99 0.23702091833531824 2307 100-101 0.10183122331062222 2201 1 0.3039047886096853 2201 2 0.26989046308872133 2201 3 0.3194523679274539 2201 4 0.3988171779905798 2201 5 0.3903041960818925 2201 6 0.34824928086892726 2201 7 0.43327628927242756 2201 8 0.39773495184034147 2201 9 0.3789578855980764 2201 10-11 0.41996043499337077 2201 12-13 0.4235988997008562 2201 14-15 0.5502311182910304 2201 16-17 0.5146591381376666 2201 18-19 0.5299093609432077 2201 20-21 0.5669712354736802 2201 22-23 0.5616276452786266 2201 24-25 0.5573954688860212 2201 26-27 0.5001626379689768 2201 28-29 0.5224790517672133 2201 30-31 0.5441463624263321 2201 32-33 0.47557728676031985 2201 34-35 0.4546217824927865 2201 36-37 0.4727244080527484 2201 38-39 0.39544680736205606 2201 40-41 0.4058533867660259 2201 42-43 0.470262153987008 2201 44-45 0.5369614314839026 2201 46-47 0.5030928266821277 2201 48-49 0.4996990289716692 2201 50-51 0.38874375861625055 2201 52-53 0.5062846385165685 2201 54-55 0.5342967254764162 2201 56-57 0.5733582726160833 2201 58-59 0.5988265060333617 2201 60-61 0.5915502950461402 2201 62-63 0.5975231597017441 2201 64-65 0.6419762021086655 2201 66-67 0.653434017577851 2201 68-69 0.5907242542117714 2201 70-71 0.5967799477286633 2201 72-73 0.40885463810717937 2201 74-75 0.4897645294675428 2201 76-77 0.5091583798052639 2201 78-79 0.5575532622489661 2201 80-81 0.5427301073733162 2201 82-83 0.5842888051983479 2201 84-85 0.5884170510272497 2201 86-87 0.5916226364185917 2201 88-89 0.48985444302532954 2201 90-91 0.5212905165851396 2201 92-93 0.5118499740819189 2201 94-95 0.4299680642685786 2201 96-97 0.47764598011323045 2201 98-99 0.4870622754297358 2201 100-101 0.33397549045065844 1101 1 0.1580695778856267 1101 2 0.16327940907653726 1101 3 0.12821378483501178 1101 4 0.18761386245499523 1101 5 0.22188301267717492 1101 6 0.26832662274357943 1101 7 0.2856802426787013 1101 8 0.1939827338340976 1101 9 0.22950186616336765 1101 10-11 0.19903339010180332 1101 12-13 0.224588984106191 1101 14-15 0.3490272109410242 1101 16-17 0.3002643210142395 1101 18-19 0.4001287722915734 1101 20-21 0.37298177148891654 1101 22-23 0.40468692771957393 1101 24-25 0.4700497823485321 1101 26-27 0.39504415210308963 1101 28-29 0.41913446966356815 1101 30-31 0.4033800261195708 1101 32-33 0.3378659552183265 1101 34-35 0.34577798057812004 1101 36-37 0.39897307114468106 1101 38-39 0.28648414132865696 1101 40-41 0.4307501029764751 1101 42-43 0.30776244443434564 1101 44-45 0.35526874700114774 1101 46-47 0.36583863866422206 1101 48-49 0.32810186433917465 1101 50-51 0.4221728241083369 1101 52-53 0.4298988860710722 1101 54-55 0.3953531990723107 1101 56-57 0.4985010518732693 1101 58-59 0.5497807718822045 1101 60-61 0.5534046153970422 1101 62-63 0.49537794161776816 1101 64-65 0.4551306864741491 1101 66-67 0.42545806192734403 1101 68-69 0.37681235837312244 1101 70-71 0.4710045249402768 1101 72-73 0.4121412130888018 1101 74-75 0.40365030384203493 1101 76-77 0.45170680431328236 1101 78-79 0.4958753512872889 1101 80-81 0.49231242351442006 1101 82-83 0.4764499242224005 1101 84-85 0.4390264068578347 1101 86-87 0.4365061071563403 1101 88-89 0.44800100842692814 1101 90-91 0.44900736635804606 1101 92-93 0.5579780953525386 1101 94-95 0.404577417521395 1101 96-97 0.4443152102349899 1101 98-99 0.5380662780357284 1101 100-101 0.4809756948468191 2304 1 0.27083956208579707 2304 2 0.20888329172765197 2304 3 0.19783047662603082 2304 4 0.25615876566748597 2304 5 0.31820840012329654 2304 6 0.3156479960962102 2304 7 0.31018810079979886 2304 8 0.28608701196974096 2304 9 0.2781707499543913 2304 10-11 0.25438383210477866 2304 12-13 0.25163914204832594 2304 14-15 0.39674937487158246 2304 16-17 0.34537557526559937 2304 18-19 0.4106334705760375 2304 20-21 0.41605314885714506 2304 22-23 0.3278684611005289 2304 24-25 0.42204873858099035 2304 26-27 0.4666442779476725 2304 28-29 0.3685927611697295 2304 30-31 0.3408537143191239 2304 32-33 0.3000092156158516 2304 34-35 0.3254290821366439 2304 36-37 0.3801932705409641 2304 38-39 0.32329307374227056 2304 40-41 0.3558085968881315 2304 42-43 0.34448425194631227 2304 44-45 0.3502834072286234 2304 46-47 0.34343780852341155 2304 48-49 0.2898145658161013 2304 50-51 0.19490496131536617 2304 52-53 0.23651499560805433 2304 54-55 0.3761157803329169 2304 56-57 0.4180910836046934 2304 58-59 0.4441691363171216 2304 60-61 0.40960781060943674 2304 62-63 0.4255130200011692 2304 64-65 0.38024371560484127 2304 66-67 0.4132760930661519 2304 68-69 0.40850336406635535 2304 70-71 0.41374257702240413 2304 72-73 0.44642480578181676 2304 74-75 0.40427684970790523 2304 76-77 0.3961472785766844 2304 78-79 0.41481903968207945 2304 80-81 0.3933231942041502 2304 82-83 0.41132648883574774 2304 84-85 0.49144834607186993 2304 86-87 0.42438239255321974 2304 88-89 0.4005784195031836 2304 90-91 0.41542343391476777 2304 92-93 0.4607637293331166 2304 94-95 0.39103492158516673 2304 96-97 0.3430805950739355 2304 98-99 0.47852529658196374 2304 100-101 0.5164846628125268 2202 1 0.14201760019326315 2202 2 0.19597776632162578 2202 3 0.16758347970412046 2202 4 0.26755456576430703 2202 5 0.31938313064690504 2202 6 0.24654586946110157 2202 7 0.2891140126334051 2202 8 0.27305427849614716 2202 9 0.28689016078836715 2202 10-11 0.2640853478335856 2202 12-13 0.23640403185564907 2202 14-15 0.3825778477337991 2202 16-17 0.35683869350036446 2202 18-19 0.37266753512072626 2202 20-21 0.38881123941646223 2202 22-23 0.3179794991772624 2202 24-25 0.3902876203593948 2202 26-27 0.42390640052976636 2202 28-29 0.34848950501170606 2202 30-31 0.3988221173323865 2202 32-33 0.328775663257737 2202 34-35 0.3085724898208255 2202 36-37 0.32716781787218707 2202 38-39 0.24360322581365068 2202 40-41 0.30907212363372594 2202 42-43 0.36251231043637233 2202 44-45 0.3831185254066227 2202 46-47 0.34188967995748953 2202 48-49 0.3615119952759116 2202 50-51 0.38588818711522066 2202 52-53 0.2984112523800988 2202 54-55 0.2981621020016689 2202 56-57 0.4384184482275586 2202 58-59 0.5060297318859099 2202 60-61 0.4910254423846183 2202 62-63 0.46449139703805287 2202 64-65 0.4836332767030598 2202 66-67 0.5387867279826821 2202 68-69 0.5225488495974133 2202 70-71 0.5334341972750991 2202 72-73 0.46107215899077403 2202 74-75 0.3608574631926018 2202 76-77 0.41742236361444185 2202 78-79 0.4233892770043397 2202 80-81 0.4980604238010429 2202 82-83 0.4982707366212793 2202 84-85 0.5190264399721443 2202 86-87 0.46171791685980423 2202 88-89 0.4561093691186642 2202 90-91 0.3605028886134818 2202 92-93 0.5108158675211243 2202 94-95 0.5091181896532788 2202 96-97 0.470170725326696 2202 98-99 0.4319764000959978 2202 100-101 0.46619654843621916 1102 1 0.11145605613785392 1102 2 0.0394974435176465 1102 3 0.08621647800468679 1102 4 0.1142685803532899 1102 5 0.1865021555140558 1102 6 0.16434073581942954 1102 7 0.1608190645044445 1102 8 0.12772814271627198 1102 9 0.11464189174934347 1102 10-11 0.15175817536895408 1102 12-13 0.11961846572790336 1102 14-15 0.1416813113832447 1102 16-17 0.013916141514762614 1102 18-19 0.13122117585022863 1102 20-21 0.10538055951753478 1102 22-23 0.13703843267045812 1102 24-25 0.12302474926763551 1102 26-27 0.16531686546958468 1102 28-29 0.10381349799646244 1102 30-31 0.11519852068460068 1102 32-33 0.17327368165437207 1102 34-35 0.12739048448673884 1102 36-37 0.02465128169492914 1102 38-39 0.0015502075377540336 1102 40-41 0.14476401191427613 1102 42-43 0.13838808253455426 1102 44-45 0.1408027302089465 1102 46-47 0.13004895919606696 1102 48-49 0.008657361122260454 1102 50-51 0.19328261751565634 1102 52-53 0.17286283847899853 1102 54-55 0.2888051575596009 1102 56-57 0.3392309231249513 1102 58-59 0.4038428504366962 1102 60-61 0.3236499883104713 1102 62-63 0.25075699569151766 1102 64-65 0.27164266349832644 1102 66-67 0.36830203446336185 1102 68-69 0.37617425440682695 1102 70-71 0.36845911375321805 1102 72-73 0.3584162474226673 1102 74-75 0.28738546230517414 1102 76-77 0.3222627989867277 1102 78-79 0.32517278657571325 1102 80-81 0.34809367936197333 1102 82-83 0.3113617860745421 1102 84-85 0.2483790716621712 1102 86-87 0.19865638007018305 1102 88-89 0.1800937544465171 1102 90-91 0.235466108128783 1102 92-93 0.33381534120675127 1102 94-95 0.3622255101166374 1102 96-97 0.09417960789014757 1102 98-99 0.2447407608907639 1102 100-101 0.32840205636761866 2305 1 0.1386624796051379 2305 2 0.1021319921092072 2305 3 0.10170368015444353 2305 4 0.11715982665874947 2305 5 0.19582140729820452 2305 6 0.15047931171224604 2305 7 0.16594161547649122 2305 8 0.12414641106284563 2305 9 0.09197515953093216 2305 10-11 0.1414046288047146 2305 12-13 0.16665012907591148 2305 14-15 0.19424013349642166 2305 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1213 80-81 -0.279675612307301 1213 82-83 -0.23154896006511905 1213 84-85 -0.281923729213851 1213 86-87 -0.2578154059097031 1213 88-89 -0.34914243050690885 1213 90-91 -0.36287598798595866 1213 92-93 -0.22128299331077272 1213 94-95 -0.2539090404040394 1213 96-97 -0.1718874218537394 1213 98-99 -0.24321227493295794 1213 100-101 -0.01718139750413883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1062.0 3 450.0 4 261.0 5 343.0 6 782.0 7 2505.0 8 8572.0 9 24383.0 10 28449.0 11 22837.0 12 15841.0 13 12329.0 14 11219.0 15 11596.0 16 11739.0 17 12242.0 18 12821.0 19 14301.0 20 16370.0 21 19009.0 22 22270.0 23 24406.0 24 27497.0 25 32392.0 26 38990.0 27 47708.0 28 59531.0 29 74808.0 30 92703.0 31 120723.0 32 167441.0 33 242883.0 34 375493.0 35 634732.0 36 1252436.0 37 2424997.0 38 1077855.0 39 775.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.43133470601046 26.73363506829735 21.477956755619356 16.357073470072834 2 31.85590575416251 24.58221929715326 29.965595166960046 13.596279781724183 3 36.09548762585345 21.23377350303064 25.381830478116157 17.288908392999755 4 30.899947887346084 20.88184187725559 30.176284429797708 18.041925805600616 5 29.883696843208273 19.620200028042934 32.5430006052888 17.95310252345999 6 26.47085456684219 19.754855889950175 35.45310825117019 18.321181292037444 7 32.097581792728796 19.819712144956913 27.961327137755493 20.121378924558798 8 29.793446704048108 27.410329077630557 23.653433227351638 19.14279099096969 9 27.54704089406348 26.964769510970743 25.602196238746544 19.885993356219227 10-11 23.328151474394048 29.45590625264834 29.95638129641982 17.259560976537795 12-13 23.598164524971576 27.41883188498482 30.183484069415776 18.79951952062783 14-15 22.396887920162172 27.657226338445817 31.21316202929875 18.73272371209326 16-17 22.105658276056595 27.947773906514183 32.06787916074247 17.87868865668675 18-19 21.867543759194685 28.081405979053358 31.942163552397705 18.108886709354252 20-21 21.7687841641314 28.84076837277473 31.202567335104774 18.187880127989096 22-23 22.31280617302909 28.753779792722405 30.452534537438257 18.48087949681024 24-25 21.6810337972167 29.100119284294234 30.50875112958612 18.710095788902947 26-27 21.497970582920434 29.483749334077796 30.34581786428064 18.672462218721133 28-29 21.69917587357106 29.362014546324026 30.111216460073564 18.82759312003135 30-31 21.90830622254451 29.080340207522493 30.165432911201773 18.84592065873122 32-33 21.339634571657875 29.418466329922115 30.378976139196723 18.86292295922328 34-35 22.110389762647618 28.94684687512062 30.226248979572695 18.716514382659067 36-37 21.457318439639863 28.757294644247338 30.74159805469419 19.043788861418605 38-39 21.250216364509136 29.234537540568116 30.55977421754693 18.955471877375814 40-41 21.39700738572217 29.638464109176628 29.756586025539498 19.207942479561705 42-43 21.53502682303594 29.016648877969924 30.21161489447542 19.236709404518717 44-45 21.07402676127815 29.12758972023612 30.49243481860721 19.305948699878517 46-47 21.393786092807606 29.351506232820658 29.868895256558897 19.385812417812833 48-49 21.035673637596407 29.542678936395795 29.787654067292834 19.633993358714967 50-51 20.953081134062604 29.190558502540014 29.920135582771266 19.93622478062612 52-53 21.711598735677473 29.04509652223282 29.370563830445022 19.872740911644684 54-55 21.822246168622105 29.226887241575994 29.229440904383292 19.721425685418605 56-57 21.808588038056357 29.161357875944084 29.374820500289907 19.655233585709656 58-59 21.586981702911764 29.21421069500686 29.491296119916743 19.707511482164637 60-61 20.83990004864857 29.3132329516338 29.40090856341029 20.44595843630734 62-63 21.050994897055876 29.179444940971678 29.507679368749102 20.261880793223344 64-65 20.73038866080075 29.32009908449898 29.756375804221353 20.193136450478917 66-67 20.61369285669051 29.016886305340094 29.690898832916645 20.67852200505275 68-69 20.440009208780538 29.08583379709006 30.413173345196277 20.060983648933128 70-71 20.274569133628578 29.993755957010293 29.668024326526094 20.06365058283504 72-73 20.264449508631387 30.42869965997762 29.204075306597833 20.10277552479316 74-75 20.43550516211824 30.40508376610897 29.081744025424598 20.07766704634819 76-77 20.44009111282168 30.348996321575495 29.11006042574754 20.10085213985528 78-79 20.29652996617917 30.30290900974093 29.378225356571946 20.022335667507953 80-81 20.16073793587374 30.5965436987511 29.253310463364258 19.989407902010907 82-83 20.050685379477724 30.46274413954358 29.227272684643484 20.25929779633521 84-85 20.22805937080886 30.042882508505915 29.43296299499871 20.296095125686517 86-87 20.352115831057873 30.015241726041246 29.509877543152367 20.122764899748518 88-89 20.592792940209176 30.27430612522595 29.07008724308196 20.062813691482916 90-91 20.624285275600755 30.120889187304122 29.03023409092827 20.224591446166848 92-93 20.40803933438948 30.229686201590912 29.000327863508385 20.361946600511224 94-95 20.14278132205211 30.302806287795974 28.96267896798354 20.591733422168375 96-97 20.228339373355293 30.56885120138302 28.84696168286235 20.355847742399337 98-99 20.54992792297183 30.483868675604175 28.66940893374259 20.29679446768141 100-101 20.675253949092724 30.330283164168613 28.35047730799388 20.64398557874478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 309.0 1 405.5 2 456.5 3 367.0 4 323.0 5 359.0 6 449.5 7 591.0 8 868.0 9 1209.5 10 1439.0 11 1637.5 12 1746.0 13 1662.0 14 1536.0 15 1353.0 16 1184.5 17 1134.5 18 1162.0 19 1303.0 20 1567.0 21 2054.0 22 2952.0 23 4443.0 24 6787.5 25 10651.5 26 17546.0 27 26818.5 28 38006.0 29 52115.0 30 69043.0 31 88241.5 32 113390.5 33 148454.0 34 190663.0 35 237527.5 36 292799.5 37 352720.5 38 407918.0 39 442597.0 40 458248.0 41 452918.5 42 426852.5 43 404418.5 44 375495.5 45 345781.5 46 329786.5 47 303182.0 48 261764.5 49 222489.5 50 182935.0 51 147747.0 52 119884.0 53 95005.0 54 77381.5 55 61452.5 56 44206.5 57 30660.0 58 21887.0 59 15962.0 60 11663.5 61 8282.0 62 5869.0 63 4114.0 64 2895.0 65 2030.5 66 1458.0 67 1085.0 68 791.0 69 581.0 70 438.5 71 335.5 72 259.0 73 220.0 74 176.0 75 149.5 76 127.0 77 98.5 78 80.5 79 64.5 80 52.0 81 36.5 82 33.5 83 31.0 84 29.5 85 29.5 86 25.0 87 21.5 88 17.0 89 11.5 90 10.5 91 10.0 92 5.5 93 7.0 94 8.0 95 5.5 96 3.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2262428127372745 2 0.21051870686220425 3 0.1854494135210895 4 0.1683861667610545 5 0.1803232398109018 6 0.1377299200504093 7 0.12413692009979913 8 0.11998990316571465 9 0.10707367333976409 10-11 0.09277510453578537 12-13 0.12016269553796817 14-15 0.11576368972768065 16-17 0.10731126285161267 18-19 0.10051476287630759 20-21 0.10489936932224064 22-23 0.09178874807750487 24-25 0.4103962834664627 26-27 0.19544977206526196 28-29 0.09265270993877246 30-31 0.17846572180917644 32-33 0.20840200030209866 34-35 0.2079772190536421 36-37 0.21365776829147654 38-39 0.21447133237750354 40-41 0.21056190495526766 42-43 0.22643720415605972 44-45 0.20899237424063152 46-47 0.22787714059150574 48-49 0.21696962209300236 50-51 0.21969110195599523 52-53 0.21189384615805518 54-55 0.19483059939802017 56-57 0.20201588221089567 58-59 0.19691850722941684 60-61 0.17824253166168233 62-63 0.16698222873649465 64-65 0.1710716482131613 66-67 0.18264153747196982 68-69 0.17620502160552626 70-71 0.16909173561442303 72-73 0.17238919005159437 74-75 0.1628352118024102 76-77 0.17318835477326688 78-79 0.17125883994976926 80-81 0.1760250295510955 82-83 0.20095032924866565 84-85 0.19923680489048493 86-87 0.20311023390183464 88-89 0.2098563361018991 90-91 0.19180673288358357 92-93 0.17261957988126572 94-95 0.17353393951777393 96-97 0.1822527546343994 98-99 0.1961841396473394 100-101 0.20771083081308458 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6944751.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.15183224394951 #Duplication Level Percentage of deduplicated Percentage of total 1 91.59737537802272 60.593342099930304 2 4.836872326106842 6.399359334040433 3 1.1799763598161177 2.341727946191461 4 0.5257704307739088 1.3912270934153874 5 0.31749556186403 1.0501456573313905 6 0.24011009924899082 0.9530233803358386 7 0.16632877779596641 0.7702067384269948 8 0.12482064442849666 0.6605691464652467 9 0.11924106365626555 0.7099213355621447 >10 0.7392565187059055 9.923231938791604 >50 0.08375723412520919 3.902375933728422 >100 0.062399511033420736 8.016202726422978 >500 0.005691383789172435 2.4830640613116945 >1k 9.047106328313825E-4 0.8056026080461083 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010511535978755754 0.0 0.0 2 0.001137549784002335 0.0 0.0 3 0.0012527446988380144 0.0 0.0 4 0.0013247415206103141 0.0 0.0 5 0.0015551313502816732 0.0 0.0 6 0.001799920544307492 0.0 0.0 7 0.002015911009624391 0.0 1.4399364354459937E-5 8 0.0024766906689671093 0.0 1.4399364354459937E-5 9 0.002779077320410768 0.0 1.4399364354459937E-5 10-11 0.0046941927795539394 0.0 1.4399364354459937E-5 12-13 0.00679649997530509 0.0 1.4399364354459937E-5 14-15 0.010050756319413035 0.0 1.4399364354459937E-5 16-17 0.01341300789617943 0.0 2.1599046531689906E-5 18-19 0.016343278542312028 1.4399364354459937E-5 2.8798728708919874E-5 20-21 0.018071202264847222 1.4399364354459937E-5 2.8798728708919874E-5 22-23 0.020058314545762693 1.4399364354459937E-5 4.319809306337981E-5 24-25 0.022239818245463373 1.4399364354459937E-5 4.319809306337981E-5 26-27 0.024421321945164054 1.4399364354459937E-5 6.479713959506971E-5 28-29 0.02727239608734712 1.4399364354459937E-5 8.639618612675963E-5 30-31 0.04636595322136099 1.4399364354459937E-5 1.0079555048121955E-4 32-33 0.05077935839600297 1.4399364354459937E-5 1.0079555048121955E-4 34-35 0.054321602027200114 1.4399364354459937E-5 1.2239459701290947E-4 36-37 0.058677409744424244 1.4399364354459937E-5 1.2959427919013942E-4 38-39 0.06367398917542184 1.4399364354459937E-5 1.4399364354459937E-4 40-41 0.06885776034302742 1.4399364354459937E-5 1.4399364354459937E-4 42-43 0.07470390227093815 1.4399364354459937E-5 1.4399364354459937E-4 44-45 0.08082363212158362 1.4399364354459937E-5 1.4399364354459937E-4 46-47 0.08791531906615514 1.4399364354459937E-5 1.4399364354459937E-4 48-49 0.09575577295715858 1.4399364354459937E-5 1.4399364354459937E-4 50-51 0.10411460396492257 1.4399364354459937E-5 1.4399364354459937E-4 52-53 0.11345259174878984 2.8798728708919874E-5 1.4399364354459937E-4 54-55 0.12307136713756908 2.8798728708919874E-5 1.4399364354459937E-4 56-57 0.1335901027985021 2.8798728708919874E-5 1.4399364354459937E-4 58-59 0.14506639618900663 2.8798728708919874E-5 1.4399364354459937E-4 60-61 0.15787463078229874 2.8798728708919874E-5 1.4399364354459937E-4 62-63 0.1724971852842528 2.8798728708919874E-5 1.5119332572182932E-4 64-65 0.1892148472997808 2.8798728708919874E-5 1.799920544307492E-4 66-67 0.20731484829333693 2.8798728708919874E-5 1.8719173660797917E-4 68-69 0.22838111834391184 2.8798728708919874E-5 1.9439141878520912E-4 70-71 0.2512473089387942 2.8798728708919874E-5 2.087907831396691E-4 72-73 0.2763310016442634 2.8798728708919874E-5 2.23190147494129E-4 74-75 0.30528092367890514 2.8798728708919874E-5 2.3758951184858898E-4 76-77 0.3385794537485938 2.8798728708919874E-5 2.4478919402581893E-4 78-79 0.3759242052018856 2.8798728708919874E-5 2.4478919402581893E-4 80-81 0.41974867061468435 2.8798728708919874E-5 2.4478919402581893E-4 82-83 0.4674249659923012 2.8798728708919874E-5 2.4478919402581893E-4 84-85 0.522207347678844 2.8798728708919874E-5 2.4478919402581893E-4 86-87 0.5840670169456039 2.8798728708919874E-5 2.4478919402581893E-4 88-89 0.6539903302508614 2.8798728708919874E-5 2.663882405575088E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1475 0.0 53.4038 9 CGGGAGA 2110 0.0 53.331264 4 AGAGCGG 1680 0.0 51.12443 8 GATCGGG 2920 0.0 50.753174 1 GAGAGCG 1950 0.0 48.913643 7 ATCGGGA 3020 0.0 44.81882 2 GATCCGG 640 0.0 39.33574 1 CGGAAAG 1195 0.0 39.335373 5 TTGTGTT 55410 0.0 38.635883 1 AGCGGCG 1335 0.0 38.0331 10-11 GGCGCCG 1535 0.0 36.503807 56-57 TCGGGAG 3195 0.0 36.26138 3 CCGGATC 940 0.0 35.8604 60-61 CGCCGTA 1625 0.0 35.502655 58-59 TGTGTTT 98645 0.0 35.497818 1 CGCCGGA 885 0.0 35.41103 58-59 GTCGCCG 1610 0.0 34.213436 56-57 TCGCCGT 1365 0.0 34.09243 56-57 CGATCGC 70 2.0544554E-4 33.928402 1 GGGCGCC 2250 0.0 33.874855 54-55 >>END_MODULE