##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422675 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861170400487065 33.0 33.0 33.0 27.0 33.0 2 31.792458336343092 33.0 33.0 33.0 33.0 33.0 3 31.74878553664854 33.0 33.0 33.0 33.0 33.0 4 35.454648271022734 37.0 37.0 37.0 33.0 37.0 5 35.40330089673605 37.0 37.0 37.0 33.0 37.0 6 35.504723497786536 37.0 37.0 37.0 33.0 37.0 7 35.39188211377919 37.0 37.0 37.0 33.0 37.0 8 35.38346084390189 37.0 37.0 37.0 33.0 37.0 9 35.28708390518745 37.0 37.0 37.0 33.0 37.0 10-11 35.23191616704676 37.0 37.0 37.0 33.0 37.0 12-13 35.22939849546472 37.0 37.0 37.0 33.0 37.0 14-15 36.90954296387258 40.0 37.0 40.0 33.0 40.0 16-17 36.8560774763433 40.0 37.0 40.0 33.0 40.0 18-19 36.856396957908096 40.0 37.0 40.0 33.0 40.0 20-21 36.830193071707924 40.0 37.0 40.0 33.0 40.0 22-23 36.93822861093626 40.0 37.0 40.0 33.0 40.0 24-25 36.68435448419619 40.0 37.0 40.0 33.0 40.0 26-27 36.568939292311185 40.0 37.0 40.0 33.0 40.0 28-29 36.51596252076733 40.0 37.0 40.0 33.0 40.0 30-31 36.507271714117664 40.0 37.0 40.0 33.0 40.0 32-33 36.42525287130961 40.0 37.0 40.0 33.0 40.0 34-35 36.383301722269806 40.0 37.0 40.0 33.0 40.0 36-37 36.29432486817258 40.0 37.0 40.0 33.0 40.0 38-39 36.23446995573075 40.0 37.0 40.0 33.0 40.0 40-41 36.13949993292538 37.0 37.0 40.0 33.0 40.0 42-43 36.01124975491966 37.0 37.0 40.0 30.0 40.0 44-45 35.892205929396226 37.0 37.0 40.0 27.0 40.0 46-47 35.80976792182195 37.0 37.0 40.0 27.0 40.0 48-49 35.68498477922132 37.0 37.0 40.0 27.0 40.0 50-51 34.52769583208644 37.0 35.0 38.5 27.0 38.5 52-53 34.96406678568112 37.0 35.0 38.5 27.0 40.0 54-55 35.757950612442855 37.0 37.0 40.0 27.0 40.0 56-57 35.82658074236123 37.0 37.0 40.0 27.0 40.0 58-59 35.579222759965745 37.0 37.0 40.0 27.0 40.0 60-61 35.335265976658036 37.0 37.0 40.0 27.0 40.0 62-63 35.11708772328109 37.0 37.0 40.0 27.0 40.0 64-65 34.85646031762411 37.0 33.0 40.0 27.0 40.0 66-67 34.59572249682685 37.0 33.0 37.0 27.0 40.0 68-69 34.390733796320184 37.0 33.0 37.0 27.0 40.0 70-71 34.06214308563881 37.0 33.0 37.0 27.0 40.0 72-73 33.85315591236959 37.0 33.0 37.0 27.0 38.5 74-75 33.607945349665144 37.0 33.0 37.0 27.0 37.0 76-77 33.35714262127607 37.0 33.0 37.0 27.0 37.0 78-79 33.104999638828986 37.0 33.0 37.0 27.0 37.0 80-81 32.90592547493989 37.0 33.0 37.0 27.0 37.0 82-83 32.715921037695935 37.0 33.0 37.0 24.5 37.0 84-85 32.54320905610534 37.0 33.0 37.0 22.0 37.0 86-87 32.39032350604188 33.0 33.0 37.0 22.0 37.0 88-89 32.2402214494309 33.0 33.0 37.0 22.0 37.0 90-91 32.10695409000382 33.0 33.0 37.0 22.0 37.0 92-93 31.91335858090747 33.0 33.0 37.0 22.0 37.0 94-95 31.78598429422023 33.0 33.0 37.0 22.0 37.0 96-97 31.652965626838103 33.0 33.0 37.0 22.0 37.0 98-99 31.50278019131745 33.0 33.0 37.0 18.5 37.0 100-101 30.2250790964533 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07058116773085032 2306 2 0.09499462962782701 2306 3 0.04708297924359428 2306 4 0.08902406197387336 2306 5 0.17046388375570842 2306 6 0.24695027325764585 2306 7 0.364212440527929 2306 8 0.17716080107611987 2306 9 0.12692395613318297 2306 10-11 0.13819013030126115 2306 12-13 0.12285347534797353 2306 14-15 0.3221019237499476 2306 16-17 0.3777916148787668 2306 18-19 0.36566036266413704 2306 20-21 0.3268532057602016 2306 22-23 0.26312074442422784 2306 24-25 0.1918556526829036 2306 26-27 0.3036736332114458 2306 28-29 0.29413915280297687 2306 30-31 0.33911742508366416 2306 32-33 0.31471380063512555 2306 34-35 0.27831418797168794 2306 36-37 0.08492345162178339 2306 38-39 0.2127278500014569 2306 40-41 0.2666689082883593 2306 42-43 0.2000109057807009 2306 44-45 0.37993970079670447 2306 46-47 0.35924958800625006 2306 48-49 0.3017043818141758 2306 50-51 0.25828327235195303 2306 52-53 0.45978482387805997 2306 54-55 0.21304112193494262 2306 56-57 0.2299867555009527 2306 58-59 0.27403071787792754 2306 60-61 0.3614138613049178 2306 62-63 0.4312566907190103 2306 64-65 0.38979861859354514 2306 66-67 0.3610533212982787 2306 68-69 0.3034673296836914 2306 70-71 0.2660212976566072 2306 72-73 0.22831411371937094 2306 74-75 0.1984577433867969 2306 76-77 0.1944591722701361 2306 78-79 0.08722845453672079 2306 80-81 0.26770981491242196 2306 82-83 0.2734466691120403 2306 84-85 0.2679199511745409 2306 86-87 0.24484316753694912 2306 88-89 0.0681172824596814 2306 90-91 0.060725752519303455 2306 92-93 0.2814404850148229 2306 94-95 0.1441180798279973 2306 96-97 0.027479177651638764 2306 98-99 0.2205375479413938 2306 100-101 0.2052920133749545 2307 1 0.26069585143424234 2307 2 0.1452933832935166 2307 3 0.31498829972955633 2307 4 0.24829354249335722 2307 5 0.33406882719935993 2307 6 0.27607941024968596 2307 7 0.46958372247178914 2307 8 0.3162477826429395 2307 9 0.34647621838705334 2307 10-11 0.2615947700163872 2307 12-13 0.26317212846904425 2307 14-15 0.525129913904955 2307 16-17 0.460437980609818 2307 18-19 0.4024301050185528 2307 20-21 0.43943529101365897 2307 22-23 0.41191983847533464 2307 24-25 0.5101219126326271 2307 26-27 0.49911050480758945 2307 28-29 0.5281749456391722 2307 30-31 0.5074272805507789 2307 32-33 0.4420722788504534 2307 34-35 0.43435406543294164 2307 36-37 0.37302501739471694 2307 38-39 0.3654968854015408 2307 40-41 0.2924487836549261 2307 42-43 0.36901763493864337 2307 44-45 0.3202549500635641 2307 46-47 0.3200851032974157 2307 48-49 0.34746317946813576 2307 50-51 0.2317671171362079 2307 52-53 0.29369545961329635 2307 54-55 0.3628033758101026 2307 56-57 0.3416454798411266 2307 58-59 0.19750448201701687 2307 60-61 0.29192640319850227 2307 62-63 0.36152271501728706 2307 64-65 0.25795896631034765 2307 66-67 0.4330845058395454 2307 68-69 0.38274466525134443 2307 70-71 0.1977999423989587 2307 72-73 0.4340405747583347 2307 74-75 0.2383213273834457 2307 76-77 0.23785242216540325 2307 78-79 0.3768880691156937 2307 80-81 0.4019372709241509 2307 82-83 0.4826403484166022 2307 84-85 0.3210651124648578 2307 86-87 0.5416431518268539 2307 88-89 0.418654960507439 2307 90-91 0.34012798335298555 2307 92-93 0.5085711668330042 2307 94-95 0.4108118900500308 2307 96-97 0.5245635146855712 2307 98-99 0.47862955673905105 2307 100-101 0.2656811000946888 2201 1 0.17814348753285714 2201 2 0.02814697826151047 2201 3 0.05429370564881708 2201 4 0.1960464894481717 2201 5 0.14008473441781888 2201 6 0.11922338325177861 2201 7 0.31291782159087234 2201 8 0.11819083194897928 2201 9 0.11572450725200412 2201 10-11 0.06272780284482593 2201 12-13 0.0729261924148048 2201 14-15 0.18088231820814826 2201 16-17 0.055022753092224264 2201 18-19 0.08407283427896317 2201 20-21 0.1734473679830444 2201 22-23 0.21112140780799393 2201 24-25 0.17036010500856946 2201 26-27 0.05701925615431236 2201 28-29 0.05475150704990739 2201 30-31 0.21241393211298742 2201 32-33 0.13288413717481262 2201 34-35 0.07400219348288317 2201 36-37 0.026241416564495523 2201 38-39 0.08944614153310937 2201 40-41 0.0745578170220611 2201 42-43 0.1186269851826367 2201 44-45 0.1895155179068979 2201 46-47 0.12198260155459195 2201 48-49 0.06795996316799346 2201 50-51 0.09736627186718039 2201 52-53 0.23903073513772455 2201 54-55 0.22835648437211375 2201 56-57 0.2403171716467014 2201 58-59 0.25290743055620624 2201 60-61 0.26409520746366866 2201 62-63 0.2585218911374483 2201 64-65 0.24083375241846738 2201 66-67 0.36628537804310923 2201 68-69 0.2578001187021357 2201 70-71 0.39333574156241013 2201 72-73 0.1941860806522442 2201 74-75 0.32071822357458757 2201 76-77 0.2018992763703622 2201 78-79 0.19246969659524638 2201 80-81 0.2350486254142936 2201 82-83 0.4093185084711095 2201 84-85 0.3333718175792555 2201 86-87 0.31620259396513717 2201 88-89 0.19615838673851016 2201 90-91 0.05725497967721793 2201 92-93 0.3316771344165055 2201 94-95 0.18484997072688358 2201 96-97 0.25160925189959116 2201 98-99 -0.027613907675679172 2201 100-101 -0.031175085341651254 1101 1 0.14212608115076364 1101 2 0.18370675083994925 1101 3 0.10888492729554144 1101 4 0.2217783909782085 1101 5 0.339078602370428 1101 6 0.22361423429660476 1101 7 0.3696020509175355 1101 8 0.2619497621150799 1101 9 0.184910969120196 1101 10-11 0.2140248164484504 1101 12-13 0.3087030424475401 1101 14-15 0.4659290341395632 1101 16-17 0.35278890925106765 1101 18-19 0.3482848215385914 1101 20-21 0.3197658139853061 1101 22-23 0.3304651708311468 1101 24-25 0.41948011155735543 1101 26-27 0.3326576700079755 1101 28-29 0.3739105272618488 1101 30-31 0.307609037637782 1101 32-33 0.2736491361329598 1101 34-35 0.3324402702227758 1101 36-37 0.32721917673000434 1101 38-39 0.2136991703477733 1101 40-41 0.3741783779853236 1101 42-43 0.41664104647334455 1101 44-45 0.28676735231185546 1101 46-47 0.3931010490452138 1101 48-49 0.4237782454505492 1101 50-51 0.3753246684558533 1101 52-53 0.27797881738455743 1101 54-55 0.4074025937964194 1101 56-57 0.5438693312585343 1101 58-59 0.5958732503454627 1101 60-61 0.622373006326562 1101 62-63 0.5672442448315635 1101 64-65 0.49380024197017747 1101 66-67 0.5035573797398385 1101 68-69 0.4122687366100948 1101 70-71 0.4414460812063865 1101 72-73 0.3928473929401477 1101 74-75 0.2818208386248955 1101 76-77 0.44664396664243355 1101 78-79 0.5006050779133417 1101 80-81 0.4864314058215058 1101 82-83 0.4017813552592244 1101 84-85 0.43952032996241286 1101 86-87 0.3899676264114049 1101 88-89 0.34400472834712303 1101 90-91 0.48932153174008164 1101 92-93 0.4777946516814886 1101 94-95 0.3930791187890357 1101 96-97 0.39994535730531666 1101 98-99 0.5369674721838216 1101 100-101 0.5797008337212759 2304 1 0.29866069669680684 2304 2 0.14516167403309765 2304 3 0.15653507839098424 2304 4 0.2880846320593804 2304 5 0.3373787786484428 2304 6 0.1981680305695832 2304 7 0.3389737457047488 2304 8 0.31793114799604894 2304 9 0.23043896374790762 2304 10-11 0.29604721319989835 2304 12-13 0.27000844410959246 2304 14-15 0.49080010460879464 2304 16-17 0.37410884335665884 2304 18-19 0.41507372861489955 2304 20-21 0.43415831062763033 2304 22-23 0.3592238011089961 2304 24-25 0.4615796037087847 2304 26-27 0.4160808474192095 2304 28-29 0.3147098378888913 2304 30-31 0.32748031796179333 2304 32-33 0.28139605651406185 2304 34-35 0.35050483204599203 2304 36-37 0.24205551623306576 2304 38-39 0.10654600783623636 2304 40-41 0.3430300263946364 2304 42-43 0.27320333661856466 2304 44-45 0.1947243897919151 2304 46-47 0.205852218985612 2304 48-49 0.39086580211133537 2304 50-51 0.3741999779916583 2304 52-53 0.3719314584069693 2304 54-55 0.4411419961299998 2304 56-57 0.5161304640546334 2304 58-59 0.5791071649765342 2304 60-61 0.5640523954392904 2304 62-63 0.46460033691583646 2304 64-65 0.48255071414342865 2304 66-67 0.5315329893679817 2304 68-69 0.5338966326060657 2304 70-71 0.4868757064057547 2304 72-73 0.4846162326530745 2304 74-75 0.518750820577381 2304 76-77 0.578594750450037 2304 78-79 0.6122688066130877 2304 80-81 0.44325837530862344 2304 82-83 0.569901922076987 2304 84-85 0.5712717517285526 2304 86-87 0.4538133201913155 2304 88-89 0.5333776822630156 2304 90-91 0.5089862362645547 2304 92-93 0.5159364138320761 2304 94-95 0.4823933133663907 2304 96-97 0.5426511986131324 2304 98-99 0.4070732652250335 2304 100-101 0.5153823108890769 2202 1 0.1259375186969578 2202 2 0.06501680257682807 2202 3 0.09917040401394317 2202 4 0.03065218994346708 2202 5 0.07252280044877324 2202 6 0.11752360236756232 2202 7 0.0467084810727485 2202 8 0.09314432974257159 2202 9 0.02665154561686478 2202 10-11 0.08596333270860868 2202 12-13 -0.019876964943442488 2202 14-15 0.057680004206076774 2202 16-17 0.15931838079578142 2202 18-19 0.09684819921782406 2202 20-21 0.15129413068893172 2202 22-23 0.11549958509574765 2202 24-25 0.20069315664235887 2202 26-27 0.2742469623213566 2202 28-29 0.18720852245771624 2202 30-31 0.23922512226453563 2202 32-33 0.20305716458825174 2202 34-35 0.1808165636447967 2202 36-37 0.12163552004115985 2202 38-39 0.16191378601666173 2202 40-41 0.2894626998626393 2202 42-43 0.09984619244525561 2202 44-45 0.21089313760379724 2202 46-47 0.28401360447757895 2202 48-49 0.13310127760131252 2202 50-51 0.19620535027403463 2202 52-53 0.3285019604000823 2202 54-55 0.23522040606544437 2202 56-57 0.39286923887124914 2202 58-59 0.431952795800008 2202 60-61 0.25465427942338437 2202 62-63 0.39651484719667707 2202 64-65 0.3185854415267144 2202 66-67 0.24991616577088394 2202 68-69 0.3523301741562861 2202 70-71 0.4213079622115643 2202 72-73 0.3572909905011272 2202 74-75 0.3180713005613285 2202 76-77 0.44485511778803044 2202 78-79 0.2612480934344106 2202 80-81 0.31431735371507585 2202 82-83 0.29625313094289396 2202 84-85 0.31572482922332057 2202 86-87 0.287782667061812 2202 88-89 0.24352431445208111 2202 90-91 0.15023478007710622 2202 92-93 0.4801101080746868 2202 94-95 0.2880902052635399 2202 96-97 0.22884756536149098 2202 98-99 0.1777437563671178 2202 100-101 0.20081941280479398 1102 1 0.21360535284806303 1102 2 0.17126231543039694 1102 3 0.1918709819385711 1102 4 0.2258762795237672 1102 5 0.2679152695791913 1102 6 0.1913605141795145 1102 7 0.2805021972828001 1102 8 0.31737035896009047 1102 9 0.32686494537649224 1102 10-11 0.3396782933010556 1102 12-13 0.24387373415893165 1102 14-15 0.25134256682780176 1102 16-17 0.21768648744805574 1102 18-19 0.2825542826975962 1102 20-21 0.22959151396362643 1102 22-23 0.15565820696168942 1102 24-25 0.21250709426457348 1102 26-27 0.1785807028859523 1102 28-29 0.28677279822731805 1102 30-31 0.25951644466873347 1102 32-33 0.17561278537132097 1102 34-35 0.2446914153954296 1102 36-37 0.1676535122588163 1102 38-39 0.2008320713614964 1102 40-41 0.22428315450725478 1102 42-43 0.2065844485170345 1102 44-45 0.22970226381507786 1102 46-47 0.30332400196438414 1102 48-49 0.28621649760536627 1102 50-51 0.1655662219604892 1102 52-53 0.3613218914616496 1102 54-55 0.2634936926200737 1102 56-57 0.2678124182103403 1102 58-59 0.2531472989712569 1102 60-61 0.2740080113409249 1102 62-63 0.2986800812819297 1102 64-65 0.40005278303375746 1102 66-67 0.3386614108831125 1102 68-69 0.31089978095335624 1102 70-71 0.36831342413477586 1102 72-73 0.38226005150252007 1102 74-75 0.3754785133145475 1102 76-77 0.37531842929218584 1102 78-79 0.3704213081946932 1102 80-81 0.3731952597001822 1102 82-83 0.367622260962051 1102 84-85 0.25864445922462664 1102 86-87 0.20493923426091953 1102 88-89 0.20951809095351592 1102 90-91 0.36004492172219216 1102 92-93 0.3069974861532181 1102 94-95 0.2920667590557464 1102 96-97 0.11083373007595654 1102 98-99 0.16105226578169507 1102 100-101 0.2893102044861706 2305 1 0.1772169180617169 2305 2 0.07884339064311519 2305 3 0.11771834222361122 2305 4 0.08712432128374559 2305 5 0.1754811531474587 2305 6 0.17458560395842682 2305 7 0.21144320479265843 2305 8 0.15718447251652634 2305 9 0.050872782782917625 2305 10-11 0.18050487438070917 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-0.3503173508550361 1213 64-65 -0.3608842720893435 1213 66-67 -0.2697052294602642 1213 68-69 -0.24498709877073566 1213 70-71 -0.23041526877995722 1213 72-73 -0.3117880663828103 1213 74-75 -0.30404371911465944 1213 76-77 -0.17627909846813594 1213 78-79 -0.10552377821550607 1213 80-81 -0.07611764891504436 1213 82-83 -0.06437556871019723 1213 84-85 -0.11532329206870173 1213 86-87 -0.10977131707753074 1213 88-89 -0.09006570572331185 1213 90-91 -0.07906247726892701 1213 92-93 -0.06136595779162235 1213 94-95 -0.03473960902969253 1213 96-97 -0.042531352358892605 1213 98-99 0.041080818484665826 1213 100-101 0.2967603648433048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 336.0 3 170.0 4 115.0 5 120.0 6 350.0 7 2470.0 8 9524.0 9 14940.0 10 14521.0 11 12781.0 12 9566.0 13 6976.0 14 5674.0 15 4995.0 16 5001.0 17 5107.0 18 5810.0 19 6327.0 20 7230.0 21 8113.0 22 9253.0 23 10322.0 24 11908.0 25 13777.0 26 16644.0 27 19844.0 28 24175.0 29 29603.0 30 37764.0 31 48913.0 32 68243.0 33 98175.0 34 149960.0 35 254924.0 36 496356.0 37 813911.0 38 198673.0 39 104.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.10578384402128 24.913938525509028 26.283352182333676 14.696925448136017 2 29.349582500464255 22.817820978746795 35.02707160544071 12.80552491534824 3 38.29535977334533 19.759468856691452 26.076450466222393 15.868720903740819 4 28.081910032416246 18.647216093274118 36.492604540423976 16.77826933388566 5 31.758352211248525 17.70086218908291 34.20202752737005 16.33875807229852 6 24.34415846085492 18.446223948915595 41.18275281305215 16.026864777177337 7 32.24404616776934 18.74894105881721 31.833238975647454 17.173773797765996 8 27.491231289008784 27.758342259071195 27.887600166613773 16.86282628530625 9 30.672104155175013 25.68250603763684 25.404144638847054 18.24124516834109 10-11 24.80076990194037 28.56802618228449 28.795927236912988 17.83527667886215 12-13 25.6188784689881 25.485734782073287 28.845050831796794 20.050335917141815 14-15 24.168233270090695 26.037930294256984 30.12087174430057 19.672964691351748 16-17 23.120949380255567 27.480160472252347 31.17137761408361 18.227512533408476 18-19 22.117685166172464 27.905306834759607 31.237411724557067 18.739596274510856 20-21 22.110099418979985 28.429448676565528 30.645836023240804 18.814615881213687 22-23 22.817910941042204 27.79984198051093 30.511745598950668 18.870501479496195 24-25 22.49050650091775 28.05023856521867 30.607981791711204 18.85127314215238 26-27 22.03888654541244 28.552083074861667 30.371971745945096 19.0370586337808 28-29 21.475224597129866 28.541383919072732 30.15096397563258 19.83242750816482 30-31 21.60258416759048 28.658123344217856 29.90889916098079 19.830393327210878 32-33 20.786265664118936 28.447140203506876 31.524870466106936 19.24172366626725 34-35 20.525940491188827 29.24984055265154 31.16966107821978 19.05455787793985 36-37 20.96446555630837 30.007677041427065 30.685788125238357 18.342069277026205 38-39 21.871611402254615 29.104356453131018 29.80743716481126 19.216594979803105 40-41 21.4531688345799 28.53846559517051 29.480269559561517 20.528096010688078 42-43 21.873248936873924 28.710537022684367 30.343135460226705 19.073078580215004 44-45 21.017320914434425 29.254588944179893 30.904743449698124 18.823346691687558 46-47 21.29557138014008 29.14729474275788 30.626342038141857 18.930791838960182 48-49 21.189802437953954 29.695402600765497 29.82617716758379 19.288617793696762 50-51 20.76225929002346 29.486868710267757 29.846920202926082 19.9039517967827 52-53 21.307479162504546 28.976848054525966 29.78670908825436 19.92896369471513 54-55 21.9672575894552 29.09615115801071 29.338683703152903 19.597907549381187 56-57 22.233644251021314 28.99113569225456 28.756132791941575 20.01908726478255 58-59 21.806854484422743 28.637579253127992 29.7527114231826 19.802854839266665 60-61 20.61040452293617 29.05894694448338 29.881236951756694 20.44941158082375 62-63 21.33617811573752 29.1599981312252 29.44827318179995 20.055550571237323 64-65 21.015981225409206 29.113164375247045 29.601465813937722 20.26938858540603 66-67 21.09920078236697 28.810291516466613 29.34395097387527 20.746556727291146 68-69 21.233683147654915 28.72898958785366 30.021064940343567 20.016262324147856 70-71 20.679005837580558 30.212363234033685 29.58155392350822 19.527077004877537 72-73 20.33263491715654 31.26602834115649 28.846207517466432 19.555129224220536 74-75 20.99145505506143 30.552896407038453 28.615426212642355 19.840222325257766 76-77 21.429634637211063 29.723967410292506 29.15408167273434 19.69231627976209 78-79 21.37710259169331 29.557645614133015 29.745234056584565 19.320017737589115 80-81 20.374410267648553 30.440567796995623 29.424905001922703 19.76011693343312 82-83 19.59460144114597 30.215386078848404 29.70197912003036 20.488033359975265 84-85 19.654350959400972 29.557862869455708 30.247299843046594 20.540486328096726 86-87 19.63156817208926 29.738958522467602 31.019101957759382 19.610371347683756 88-89 19.9590306752907 30.27111931462725 30.380977493756884 19.38887251632517 90-91 20.355075271689795 29.611448128639466 29.851408560497944 20.18206803917279 92-93 19.727834480363356 30.58505766849351 29.6707965260392 20.016311325103935 94-95 19.269339878331504 31.071609761099573 29.810311101040753 19.84873925952817 96-97 19.847745699756754 31.585468852892863 29.202391327045916 19.364394120304468 98-99 20.414620806309365 30.476604042402943 28.750068496903943 20.358706654383752 100-101 20.295601303048 30.26410049815611 28.36604878323178 21.07424941556411 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 183.0 1 189.5 2 193.5 3 220.5 4 206.0 5 183.5 6 216.5 7 301.0 8 418.5 9 502.5 10 597.5 11 682.5 12 742.0 13 735.0 14 680.0 15 626.5 16 606.5 17 613.0 18 622.0 19 721.5 20 868.5 21 1050.5 22 1357.5 23 1787.0 24 2378.0 25 3317.5 26 4675.5 27 6441.5 28 8332.5 29 10292.0 30 12558.5 31 15184.5 32 20879.5 33 31457.5 34 47582.5 35 70763.0 36 102606.5 37 142053.5 38 182639.0 39 202182.5 40 197966.5 41 176358.0 42 150209.5 43 141114.0 44 134455.5 45 125446.5 46 123600.5 47 110872.0 48 83026.0 49 62377.0 50 50117.5 51 41419.5 52 34901.0 53 28007.0 54 21328.0 55 15609.0 56 11355.5 57 8136.5 58 6119.5 59 4777.5 60 3746.5 61 2946.5 62 2276.5 63 1774.5 64 1409.5 65 1134.0 66 910.0 67 717.0 68 589.5 69 477.0 70 391.0 71 324.0 72 258.0 73 198.5 74 155.5 75 130.0 76 96.5 77 70.5 78 57.0 79 51.5 80 41.0 81 32.5 82 27.5 83 16.5 84 12.5 85 12.0 86 12.5 87 11.5 88 6.0 89 4.5 90 5.5 91 5.5 92 3.5 93 3.5 94 3.5 95 3.5 96 2.5 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21534048107979814 2 0.199283849463919 3 0.1731144292981931 4 0.1579246081294437 5 0.16774845986358056 6 0.13002156706945833 7 0.11578110972375577 8 0.11173599430381706 9 0.10071511861888202 10-11 0.08696998152868214 12-13 0.11280918819073958 14-15 0.10857832767498736 16-17 0.10085958702673699 18-19 0.09361552829001 20-21 0.09751617530209376 22-23 0.08616508611349026 24-25 0.40071408670168307 26-27 0.19049191492874612 28-29 0.0920470141475848 30-31 0.17567358395162372 32-33 0.20506258577811715 34-35 0.20427832870690454 36-37 0.20956174476560002 38-39 0.21053174693262613 40-41 0.20751854871165135 42-43 0.22262581650448368 44-45 0.20481492565036583 46-47 0.2232862435118206 48-49 0.2128226031143261 50-51 0.21641367496672068 52-53 0.20795195393521626 54-55 0.1918953223193371 56-57 0.1994695945597325 58-59 0.1944338386287884 60-61 0.17526081707203814 62-63 0.16419866469914454 64-65 0.1686978236866274 66-67 0.18048231809879575 68-69 0.1734240044578823 70-71 0.1665927126007409 72-73 0.16954399578977783 74-75 0.16000908087135088 76-77 0.1700599543892598 78-79 0.16851207859081388 80-81 0.17356847286573723 82-83 0.19843767736076856 84-85 0.19647703468273706 86-87 0.20060470347859288 88-89 0.206899398392273 90-91 0.18855191059469387 92-93 0.16964718750967422 94-95 0.17072038139659673 96-97 0.1796567843396246 98-99 0.19327809136594878 100-101 0.20469109558649015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422675.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.36597206728871 #Duplication Level Percentage of deduplicated Percentage of total 1 88.82843491889037 38.52131427473573 2 4.665768929408301 4.046712101302878 3 1.950006851036575 2.5369182789922116 4 0.9429433068186662 1.635666124181405 5 0.5681142526448901 1.2318413405613453 6 0.3899611404518447 1.014662635449765 7 0.3038519665309934 0.9223785125221661 8 0.24218843228829065 0.8402189431707565 9 0.18874884750581608 0.736674952380314 >10 1.5056793162631428 12.700944106288215 >50 0.1989173100401957 6.036623887118433 >100 0.1827516627030588 17.136594358677527 >500 0.025632258924317933 7.528571825192783 >1k 0.006904175328595397 4.875623742411186 >5k 9.663116494855415E-5 0.23525491701535817 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5614 0.23172732619934577 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4683 0.19329872970992806 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4330 0.17872805886055704 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3818 0.15759439462577524 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3688 0.15222842519116267 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3511 0.14492245142249785 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3070 0.1267194320327737 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2987 0.12329346693221335 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2868 0.11838154106514494 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2795 0.11536834284417018 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2613 0.10785598563571258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.842570712126059E-4 0.0 0.0 2 8.668104471297223E-4 0.0 0.0 3 9.90640511005397E-4 0.0 4.1276687958558206E-5 4 0.0010731938869225134 0.0 4.1276687958558206E-5 5 0.0012383006387567462 0.0 4.1276687958558206E-5 6 0.0014446840785495372 0.0 4.1276687958558206E-5 7 0.0016923442063008865 0.0 1.2383006387567462E-4 8 0.0018574509581351193 0.0 1.2383006387567462E-4 9 0.0020638343979279103 0.0 1.2383006387567462E-4 10-11 0.0032195816607675396 0.0 1.2383006387567462E-4 12-13 0.004519797331462124 0.0 1.2383006387567462E-4 14-15 0.006047034785928777 0.0 1.2383006387567462E-4 16-17 0.007388527144581919 0.0 1.2383006387567462E-4 18-19 0.00899831797496569 0.0 1.2383006387567462E-4 20-21 0.010546193773411622 0.0 1.6510675183423282E-4 22-23 0.012589389827360253 0.0 1.6510675183423282E-4 24-25 0.015004076072935909 0.0 1.6510675183423282E-4 26-27 0.01746003900647012 0.0 1.6510675183423282E-4 28-29 0.020535152259382707 0.0 1.6510675183423282E-4 30-31 0.03366113903020422 0.0 1.6510675183423282E-4 32-33 0.037747531138101476 0.0 2.0638343979279103E-4 34-35 0.0413798796784546 0.0 2.0638343979279103E-4 36-37 0.045590101850227535 0.0 2.0638343979279103E-4 38-39 0.05019245255760678 0.0 2.4766012775134924E-4 40-41 0.05448522810529683 0.0 2.4766012775134924E-4 42-43 0.059686090788075165 0.0 2.4766012775134924E-4 44-45 0.06490759181483277 0.0 2.4766012775134924E-4 46-47 0.0713054784484093 0.0 2.4766012775134924E-4 48-49 0.0783225154013642 0.0 2.4766012775134924E-4 50-51 0.08519508394646413 0.0 2.4766012775134924E-4 52-53 0.09264552612298389 0.0 2.4766012775134924E-4 54-55 0.10026107505133788 0.0 2.4766012775134924E-4 56-57 0.10954832984201347 0.0 2.4766012775134924E-4 58-59 0.11893877635258547 0.0 2.4766012775134924E-4 60-61 0.12865943636682592 0.0 2.4766012775134924E-4 62-63 0.1396596737077817 0.0 2.4766012775134924E-4 64-65 0.15167118990372214 0.0 2.4766012775134924E-4 66-67 0.16529249693004633 0.0 2.4766012775134924E-4 68-69 0.18021401962706513 0.0 2.8893681570990744E-4 70-71 0.19695171659426047 0.0 3.0957515968918655E-4 72-73 0.2151134592960261 0.0 3.3021350366846565E-4 74-75 0.23344030874962593 0.0 3.3021350366846565E-4 76-77 0.254739079736242 0.0 3.3021350366846565E-4 78-79 0.2775238114893661 0.0 3.3021350366846565E-4 80-81 0.30323918808754785 0.0 3.3021350366846565E-4 82-83 0.33215350800251786 0.0 3.3021350366846565E-4 84-85 0.3640191111065248 0.0 3.3021350366846565E-4 86-87 0.3998059995665948 0.0 3.3021350366846565E-4 88-89 0.4386267245916188 0.0 3.3021350366846565E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 500 0.0 80.68282 9 CCGAGCG 25 5.280436E-5 75.988594 2 AGAGCGG 595 0.0 68.59834 8 CGGGAGA 915 0.0 68.50156 4 CGGAAAG 240 0.0 63.310734 5 GAGAGCG 800 0.0 61.699898 7 ATTCGGA 115 0.0 57.817413 2 TCGGGAG 1085 0.0 54.70624 3 GATCCGG 245 0.0 54.28992 1 TTGTGTT 30550 0.0 52.68166 1 GAACCGG 150 0.0 47.50368 1 TCGGGAA 285 0.0 46.651947 3 ATCGGGA 1525 0.0 46.091446 2 ACCGGAA 115 1.8189894E-12 45.420452 3 CCGGAAG 120 1.8189894E-12 43.527035 4 GGTACGG 55 4.962673E-5 43.185165 1 CGGAGAG 450 0.0 42.207157 5 CCGGAGA 340 0.0 41.89768 4 GGACCGG 200 0.0 40.378128 1 GATCGGG 1915 0.0 39.441696 1 >>END_MODULE