FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422675
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56140.23172732619934577No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46830.19329872970992806No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC43300.17872805886055704No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA38180.15759439462577524No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG36880.15222842519116267No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35110.14492245142249785No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30700.1267194320327737No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC29870.12329346693221335No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT28680.11838154106514494No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27950.11536834284417018No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG26130.10785598563571258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC5000.080.682829
CCGAGCG255.280436E-575.9885942
AGAGCGG5950.068.598348
CGGGAGA9150.068.501564
CGGAAAG2400.063.3107345
GAGAGCG8000.061.6998987
ATTCGGA1150.057.8174132
TCGGGAG10850.054.706243
GATCCGG2450.054.289921
TTGTGTT305500.052.681661
GAACCGG1500.047.503681
TCGGGAA2850.046.6519473
ATCGGGA15250.046.0914462
ACCGGAA1151.8189894E-1245.4204523
CCGGAAG1201.8189894E-1243.5270354
GGTACGG554.962673E-543.1851651
CGGAGAG4500.042.2071575
CCGGAGA3400.041.897684
GGACCGG2000.040.3781281
GATCGGG19150.039.4416961
Produced by FastQC (version 0.11.2)