##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2643498 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09707024556099 33.0 33.0 33.0 33.0 33.0 2 32.05047213956659 33.0 33.0 33.0 33.0 33.0 3 32.03492796287343 33.0 33.0 33.0 33.0 33.0 4 35.79509914514783 37.0 37.0 37.0 33.0 37.0 5 35.74306014228117 37.0 37.0 37.0 33.0 37.0 6 35.81819278849464 37.0 37.0 37.0 33.0 37.0 7 35.74424985379221 37.0 37.0 37.0 33.0 37.0 8 35.69839356791645 37.0 37.0 37.0 33.0 37.0 9 35.64413515728024 37.0 37.0 37.0 33.0 37.0 10-11 35.58629191321499 37.0 37.0 37.0 33.0 37.0 12-13 35.588405022436184 37.0 37.0 37.0 33.0 37.0 14-15 37.355481638344344 40.0 37.0 40.0 33.0 40.0 16-17 37.32762063750379 40.0 37.0 40.0 33.0 40.0 18-19 37.30891436271183 40.0 37.0 40.0 33.0 40.0 20-21 37.26579063044496 40.0 37.0 40.0 33.0 40.0 22-23 37.309953516136574 40.0 37.0 40.0 33.0 40.0 24-25 37.117686300500324 40.0 37.0 40.0 33.0 40.0 26-27 37.019115202659506 40.0 37.0 40.0 33.0 40.0 28-29 36.974463003187445 40.0 37.0 40.0 33.0 40.0 30-31 36.957472636635245 40.0 37.0 40.0 33.0 40.0 32-33 36.87251002270476 40.0 37.0 40.0 33.0 40.0 34-35 36.848431699210664 40.0 37.0 40.0 33.0 40.0 36-37 36.76157708460532 40.0 37.0 40.0 33.0 40.0 38-39 36.70153183395637 40.0 37.0 40.0 33.0 40.0 40-41 36.57768116336763 40.0 37.0 40.0 33.0 40.0 42-43 36.48997010778899 40.0 37.0 40.0 33.0 40.0 44-45 36.38762257433143 38.5 37.0 40.0 33.0 40.0 46-47 36.28866619153864 37.0 37.0 40.0 33.0 40.0 48-49 36.161862804511294 37.0 37.0 40.0 33.0 40.0 50-51 34.982166243363906 37.0 35.0 38.5 27.0 38.5 52-53 35.40407255840557 37.0 37.0 38.5 30.0 40.0 54-55 36.19812195810248 37.0 37.0 40.0 33.0 40.0 56-57 36.2487333071559 37.0 37.0 40.0 33.0 40.0 58-59 36.03138511926243 37.0 37.0 40.0 33.0 40.0 60-61 35.7982733484194 37.0 37.0 40.0 27.0 40.0 62-63 35.55775794042591 37.0 37.0 40.0 27.0 40.0 64-65 35.296651822698564 37.0 37.0 40.0 27.0 40.0 66-67 35.02655307475172 37.0 35.0 37.0 27.0 40.0 68-69 34.800808814684174 37.0 33.0 37.0 27.0 40.0 70-71 34.48425173765972 37.0 33.0 37.0 27.0 40.0 72-73 34.27477437092821 37.0 33.0 37.0 27.0 38.5 74-75 34.022345581498456 37.0 33.0 37.0 27.0 37.0 76-77 33.77240535078899 37.0 33.0 37.0 27.0 37.0 78-79 33.53270836596056 37.0 33.0 37.0 27.0 37.0 80-81 33.32288732580845 37.0 33.0 37.0 27.0 37.0 82-83 33.12647408849941 37.0 33.0 37.0 27.0 37.0 84-85 32.96627933896678 37.0 33.0 37.0 27.0 37.0 86-87 32.80901820239697 37.0 33.0 37.0 27.0 37.0 88-89 32.64494771700224 37.0 33.0 37.0 27.0 37.0 90-91 32.52794176504011 33.0 33.0 37.0 24.5 37.0 92-93 32.362192443497214 33.0 33.0 37.0 22.0 37.0 94-95 32.232236226393965 33.0 33.0 37.0 22.0 37.0 96-97 32.08856163310885 33.0 33.0 37.0 22.0 37.0 98-99 31.939502318518873 33.0 33.0 37.0 22.0 37.0 100-101 30.62373340172756 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.273852276452466 2306 2 0.23117102576930648 2306 3 0.15516755104856372 2306 4 0.25251741376469994 2306 5 0.22451436425587445 2306 6 0.23537641618108296 2306 7 0.2535157508574528 2306 8 0.15540401980414487 2306 9 0.284503556280427 2306 10-11 0.15884709732147684 2306 12-13 0.18045376983793204 2306 14-15 0.3327214402107117 2306 16-17 0.350335168798253 2306 18-19 0.3363415972801249 2306 20-21 0.3582918153894923 2306 22-23 0.3689143299558353 2306 24-25 0.19257573580710385 2306 26-27 0.2450698063858212 2306 28-29 0.1896563427304585 2306 30-31 0.25069745381176745 2306 32-33 0.28573296967031325 2306 34-35 0.21477690200465105 2306 36-37 0.3029105780723853 2306 38-39 0.10451871073075125 2306 40-41 0.043564445003845265 2306 42-43 0.10566059326091448 2306 44-45 0.2182936510922886 2306 46-47 0.32447412720588176 2306 48-49 0.339425899178309 2306 50-51 0.3635444913801251 2306 52-53 0.20838432241645677 2306 54-55 0.20874524837383746 2306 56-57 0.22162130182784523 2306 58-59 0.2616178762521386 2306 60-61 0.2704169939507537 2306 62-63 0.2483346427192501 2306 64-65 0.2470201125286806 2306 66-67 0.21029020887665695 2306 68-69 0.25324408397862896 2306 70-71 0.4306257392804369 2306 72-73 0.29094278182448363 2306 74-75 0.40555761598351836 2306 76-77 0.39175946749535484 2306 78-79 0.2315461692169194 2306 80-81 0.2704979190213237 2306 82-83 0.360495505528462 2306 84-85 0.31286213994086154 2306 86-87 0.2928134965212976 2306 88-89 0.2641774253243838 2306 90-91 0.1800860887980349 2306 92-93 0.24828445133940846 2306 94-95 0.1616633382731294 2306 96-97 -0.0049208340774455905 2306 98-99 0.06527599701449205 2306 100-101 0.15930541770663353 2307 1 0.06258575240003239 2307 2 0.0378693235404981 2307 3 0.0600703722505429 2307 4 0.05605717205186522 2307 5 0.14591564047037764 2307 6 0.08712892564298613 2307 7 0.15476819632579009 2307 8 0.1118774259073021 2307 9 0.1978809537535966 2307 10-11 0.08485225337435054 2307 12-13 0.0834518683148815 2307 14-15 0.16140975498591814 2307 16-17 0.18749668855397772 2307 18-19 0.30617548827165564 2307 20-21 0.2946881549576261 2307 22-23 0.15675052471568307 2307 24-25 0.25290109737074573 2307 26-27 0.22664605928839165 2307 28-29 0.15818567920862137 2307 30-31 0.20780119652107487 2307 32-33 0.27067071307440216 2307 34-35 0.21459452034703475 2307 36-37 0.2993315094529976 2307 38-39 0.11407432113794158 2307 40-41 0.14498418793255752 2307 42-43 0.1515878600043692 2307 44-45 0.1407723046677063 2307 46-47 0.12382413543362958 2307 48-49 0.12018166317004386 2307 50-51 -0.0445538941536725 2307 52-53 0.17623304163097941 2307 54-55 0.15439825698554444 2307 56-57 -0.013429801604040392 2307 58-59 0.07012902027422996 2307 60-61 0.12838922074394787 2307 62-63 0.05215368720391922 2307 64-65 0.1702817730702506 2307 66-67 0.16924199429710285 2307 68-69 0.13021280026772786 2307 70-71 0.12925993378430434 2307 72-73 0.18907142717215208 2307 74-75 0.09383536633548317 2307 76-77 0.14364636344181747 2307 78-79 0.23166272892292028 2307 80-81 0.1271655913011429 2307 82-83 0.09533451600511711 2307 84-85 0.240527928981507 2307 86-87 0.19999677408880956 2307 88-89 0.2510409179102666 2307 90-91 0.10603319354635943 2307 92-93 0.05692952474916524 2307 94-95 0.0322765794557327 2307 96-97 -0.029047321927812675 2307 98-99 -0.004414365554762156 2307 100-101 -0.1281856689040879 2201 1 0.22926269128036125 2201 2 0.18534578305085603 2201 3 0.18680038423479317 2201 4 0.2790840510109476 2201 5 0.2410192186248068 2201 6 0.2809810057662574 2201 7 0.31769492120167087 2201 8 0.28109245757995893 2201 9 0.2529545712848389 2201 10-11 0.15314321382633267 2201 12-13 0.21429975395091105 2201 14-15 0.373621704994477 2201 16-17 0.31695696932781914 2201 18-19 0.34619817274349174 2201 20-21 0.41753056207969763 2201 22-23 0.4253132708208014 2201 24-25 0.3630611727003128 2201 26-27 0.34618851777152315 2201 28-29 0.424115742553937 2201 30-31 0.39403814224954914 2201 32-33 0.4058543288936107 2201 34-35 0.25233206528796615 2201 36-37 0.3902097837211045 2201 38-39 0.33093530384638115 2201 40-41 0.38289807254135155 2201 42-43 0.37293994012763676 2201 44-45 0.44404828480809755 2201 46-47 0.4071638889004987 2201 48-49 0.29749077119949163 2201 50-51 0.26969409420448187 2201 52-53 0.36732960043940466 2201 54-55 0.43192265349299674 2201 56-57 0.4179650794094911 2201 58-59 0.5885132866669665 2201 60-61 0.5633759524679718 2201 62-63 0.6404484997360171 2201 64-65 0.4803232899337999 2201 66-67 0.5264684966083379 2201 68-69 0.48349562855055694 2201 70-71 0.5363207966502515 2201 72-73 0.4842349265729382 2201 74-75 0.43734931942570654 2201 76-77 0.4523353721732022 2201 78-79 0.38976108536873255 2201 80-81 0.46101424735318375 2201 82-83 0.5416583475408174 2201 84-85 0.5224693773636275 2201 86-87 0.2999869298840494 2201 88-89 0.4489766309730996 2201 90-91 0.27969994581480506 2201 92-93 0.5192553251258119 2201 94-95 0.2034925527479743 2201 96-97 0.21750635038857524 2201 98-99 0.27898958924749806 2201 100-101 0.05881998081342843 1101 1 0.12001291193349317 1101 2 0.14379238499260794 1101 3 0.0925903224519189 1101 4 0.142190847127452 1101 5 0.24146052489135883 1101 6 0.20770651326846234 1101 7 0.16322448872152506 1101 8 0.16965821221367605 1101 9 0.13212050244106166 1101 10-11 0.09298213703903713 1101 12-13 0.12809278131189217 1101 14-15 0.33102241108450414 1101 16-17 0.2582124856561592 1101 18-19 0.3481465399103101 1101 20-21 0.23071017373018776 1101 22-23 0.2652073573168252 1101 24-25 0.368215629893605 1101 26-27 0.35416071547673056 1101 28-29 0.30265281228032137 1101 30-31 0.22013699485325589 1101 32-33 0.2329527401910525 1101 34-35 0.21082721091904233 1101 36-37 0.2802936319117535 1101 38-39 0.1622195050820352 1101 40-41 0.31415138233835194 1101 42-43 0.11991478567044567 1101 44-45 0.15000150634383402 1101 46-47 0.15476980240446636 1101 48-49 0.15694575796030819 1101 50-51 0.3024235734630878 1101 52-53 0.2958070938198105 1101 54-55 0.2659826711452453 1101 56-57 0.30997081639095114 1101 58-59 0.43297709955310637 1101 60-61 0.3417541519384031 1101 62-63 0.4011590028251675 1101 64-65 0.3402019307104993 1101 66-67 0.34881624594638794 1101 68-69 0.17804549615867415 1101 70-71 0.29450482136339673 1101 72-73 0.11208135199217395 1101 74-75 0.1277332558776081 1101 76-77 0.21673740218202653 1101 78-79 0.4362019503290142 1101 80-81 0.3854361361440013 1101 82-83 0.383377235449025 1101 84-85 0.27861677365665116 1101 86-87 0.35830334647716455 1101 88-89 0.47878466274715237 1101 90-91 0.3810569625844451 1101 92-93 0.39499671965361927 1101 94-95 0.29842415027665936 1101 96-97 0.14838896292167192 1101 98-99 0.2771471355846593 1101 100-101 0.3418958854912759 2304 1 0.18181494349741456 2304 2 0.21756752778657074 2304 3 0.22427899500949167 2304 4 0.23273486946623478 2304 5 0.26250611162007687 2304 6 0.2365156387310563 2304 7 0.32345204117666526 2304 8 0.3743535819304711 2304 9 0.1949342954712634 2304 10-11 0.25742447725563267 2304 12-13 0.3146121929085979 2304 14-15 0.33958900585005125 2304 16-17 0.2693530508998805 2304 18-19 0.33213861498281716 2304 20-21 0.36379350304933666 2304 22-23 0.27825111509942957 2304 24-25 0.43803323973941843 2304 26-27 0.28289174471218104 2304 28-29 0.4201384209928207 2304 30-31 0.3398122564035546 2304 32-33 0.4030384715665818 2304 34-35 0.44981712213057534 2304 36-37 0.3793856637070405 2304 38-39 0.36903957387801967 2304 40-41 0.4172278768152893 2304 42-43 0.3350312984899446 2304 44-45 0.3744934541638756 2304 46-47 0.4373661586977349 2304 48-49 0.37527771888867534 2304 50-51 0.30178740592070596 2304 52-53 0.3475102856759591 2304 54-55 0.43910747983982645 2304 56-57 0.47643999834530604 2304 58-59 0.4818795991254561 2304 60-61 0.4890556219604889 2304 62-63 0.5416757919968305 2304 64-65 0.5157544928091369 2304 66-67 0.4146186704568393 2304 68-69 0.565656623979713 2304 70-71 0.46275563276385867 2304 72-73 0.4951014343668163 2304 74-75 0.5227438969152587 2304 76-77 0.44159612538646087 2304 78-79 0.4487155526891229 2304 80-81 0.5315139152830213 2304 82-83 0.4591880385202245 2304 84-85 0.5308076591935276 2304 86-87 0.43575098426541814 2304 88-89 0.42777111860836925 2304 90-91 0.45009247228275484 2304 92-93 0.4276502542190741 2304 94-95 0.3427087694921269 2304 96-97 0.38053170492231203 2304 98-99 0.31480286814737113 2304 100-101 0.404017660228984 2202 1 0.2681426614128952 2202 2 0.2682615941391546 2202 3 0.2348011309213689 2202 4 0.35274468196110575 2202 5 0.40857568679729184 2202 6 0.36942976439981834 2202 7 0.33269678791811685 2202 8 0.27295450723461556 2202 9 0.36074904580416245 2202 10-11 0.37606415838485674 2202 12-13 0.3611165357317532 2202 14-15 0.46861237141224166 2202 16-17 0.3814674229399202 2202 18-19 0.34956152909966676 2202 20-21 0.47078658224023684 2202 22-23 0.38808161130987884 2202 24-25 0.4506587086345988 2202 26-27 0.4543260014377566 2202 28-29 0.4320852217400457 2202 30-31 0.3585107948536148 2202 32-33 0.36406274778478576 2202 34-35 0.3065648448287561 2202 36-37 0.3001659158846337 2202 38-39 0.20421419128337703 2202 40-41 0.38744166037036365 2202 42-43 0.3415889041000142 2202 44-45 0.41735517299177616 2202 46-47 0.2861609954900075 2202 48-49 0.3013385404733384 2202 50-51 0.3522388953991822 2202 52-53 0.45789988231359047 2202 54-55 0.3762030343031526 2202 56-57 0.4017213795774879 2202 58-59 0.3526158627356608 2202 60-61 0.4504438076107746 2202 62-63 0.48010035711888577 2202 64-65 0.3384402397191266 2202 66-67 0.44012912755849953 2202 68-69 0.44511127163437436 2202 70-71 0.4465788701988771 2202 72-73 0.3568595298956865 2202 74-75 0.3907458099987906 2202 76-77 0.48528431747840983 2202 78-79 0.4017934728990653 2202 80-81 0.455914378023337 2202 82-83 0.4355742519649297 2202 84-85 0.584469564035274 2202 86-87 0.5559422220251733 2202 88-89 0.45203851262328243 2202 90-91 0.4714000578177959 2202 92-93 0.6447892060293086 2202 94-95 0.5124747256059194 2202 96-97 0.42153099104565683 2202 98-99 0.5225984188162833 2202 100-101 0.5213969828668148 1102 1 0.1554772963474207 1102 2 0.17329660272299208 1102 3 0.19200902804999487 1102 4 0.19048494519872605 1102 5 0.27954380878874474 1102 6 0.24522839771856297 1102 7 0.26936407172328103 1102 8 0.2618897750166198 1102 9 0.19880404967530296 1102 10-11 0.22117600071157284 1102 12-13 0.24744804009099397 1102 14-15 0.2721611982880674 1102 16-17 0.22643535978981788 1102 18-19 0.23010651849610753 1102 20-21 0.23128242497089957 1102 22-23 0.1638192320726617 1102 24-25 0.20166773007736083 1102 26-27 0.2618591586061001 1102 28-29 0.2575956233288963 1102 30-31 0.31721623464894577 1102 32-33 0.3131084272542708 1102 34-35 0.19512267631628788 1102 36-37 0.14094376285672183 1102 38-39 0.2258719655453305 1102 40-41 0.3362251962373364 1102 42-43 0.25476929929306635 1102 44-45 0.24475990924922542 1102 46-47 0.14147834493626732 1102 48-49 0.11261744780157557 1102 50-51 0.2447537067231167 1102 52-53 0.3331157405288181 1102 54-55 0.3307192257731728 1102 56-57 0.4595410853707378 1102 58-59 0.4993068783212138 1102 60-61 0.4527462952399546 1102 62-63 0.4143978290951864 1102 64-65 0.3649693406044392 1102 66-67 0.49364807932071386 1102 68-69 0.5505224541839411 1102 70-71 0.5169579054430997 1102 72-73 0.39282285503791314 1102 74-75 0.41506541480573844 1102 76-77 0.39597918737438675 1102 78-79 0.42893396167275455 1102 80-81 0.4614278092011759 1102 82-83 0.5600041001488592 1102 84-85 0.5789969481534953 1102 86-87 0.4070025781701929 1102 88-89 0.3895056920759359 1102 90-91 0.5828296030428248 1102 92-93 0.6189274562733544 1102 94-95 0.44207675543053426 1102 96-97 0.2054981536672642 1102 98-99 0.3263256548212716 1102 100-101 0.49795932507572616 2305 1 0.13601919589319067 2305 2 0.09296265876351129 2305 3 0.11759130369161852 2305 4 0.13654578350118385 2305 5 0.17830542158945661 2305 6 0.19603093493248736 2305 7 0.17882083253852699 2305 8 0.20685055163338006 2305 9 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52-53 -0.46234970492955796 1213 54-55 -0.4682534922003967 1213 56-57 -0.5380093197545719 1213 58-59 -0.4375106223741838 1213 60-61 -0.4352744679927909 1213 62-63 -0.41894021499751943 1213 64-65 -0.3899343355824456 1213 66-67 -0.4026388328438131 1213 68-69 -0.4295846819435525 1213 70-71 -0.48108466187589727 1213 72-73 -0.5909305210063067 1213 74-75 -0.4490593485346821 1213 76-77 -0.3195655334346057 1213 78-79 -0.24822928632853802 1213 80-81 -0.29346005229353267 1213 82-83 -0.37500794593582043 1213 84-85 -0.3653404947565946 1213 86-87 -0.3226357252386052 1213 88-89 -0.36143275848801437 1213 90-91 -0.3579057913598902 1213 92-93 -0.3007583585085598 1213 94-95 -0.27676482397491853 1213 96-97 -0.1324608088889292 1213 98-99 -0.13954489351111832 1213 100-101 0.10823158453537474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 371.0 3 159.0 4 89.0 5 137.0 6 365.0 7 1457.0 8 4298.0 9 10351.0 10 11636.0 11 9966.0 12 7157.0 13 5617.0 14 4988.0 15 4850.0 16 5072.0 17 5314.0 18 5721.0 19 6462.0 20 7345.0 21 8591.0 22 9698.0 23 10732.0 24 12043.0 25 14192.0 26 16954.0 27 20292.0 28 24914.0 29 30959.0 30 38812.0 31 50425.0 32 69943.0 33 101120.0 34 155522.0 35 262518.0 36 518461.0 37 928964.0 38 277155.0 39 848.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.56463391007868 25.100005042430347 23.98502132796384 14.350339719527133 2 32.82222774814334 22.16687035077132 32.51696693645966 12.493934964625682 3 39.03834289101214 18.49634443418693 26.08272356706823 16.382589107732702 4 31.66618356115074 17.742248998266504 33.856640806312576 16.734926634270177 5 30.932177376997743 16.656321266731695 35.48669232932175 16.92480902694882 6 25.227298382300688 17.164825324384584 40.653966291428304 16.95391000188642 7 33.818002498968866 17.190921145374617 30.222865754468547 18.768210601187977 8 30.730189629640854 26.463693138565603 24.919387519362523 17.886729712431023 9 29.71949720496918 26.781363532743253 24.81155032002175 18.68758894226582 10-11 24.51380364497708 28.12205380002529 30.216226940601583 17.147915614396048 12-13 25.18201732459529 25.84602455378108 29.80192833834771 19.17002978327592 14-15 23.449596403113645 26.78087078764449 30.860657010436636 18.90887579880523 16-17 22.58583918520179 27.971293782983032 31.59047119911298 17.8523958327022 18-19 22.150896014189005 28.015618054025154 31.51097459292564 18.3225113388602 20-21 22.121910138313414 28.350217016419936 31.059059820191802 18.468813025074844 22-23 22.2952059195359 27.741690844869648 31.113589405839043 18.849513829755413 24-25 22.191382578473178 27.84062713591965 31.0204803299308 18.947509955676374 26-27 21.745976638140572 28.379061121443538 30.99143686540684 18.88352537500905 28-29 21.359186173198502 28.58934857696738 30.432612895561604 19.618852354272505 30-31 21.273189687202944 28.90770848858177 30.293448198701324 19.525653625513968 32-33 20.7226689693497 29.138063183782766 31.36909694239982 18.770170904467715 34-35 21.46402436597482 28.789339317426716 31.195877343775173 18.550758972823296 36-37 20.978227823532936 29.10562504122513 31.516451765269597 18.399695369972335 38-39 21.256579989855652 29.00950065998919 30.831787293180014 18.902132056975148 40-41 21.272847402964164 28.996916657253134 29.99530713262771 19.73492880715499 42-43 21.335406676161377 28.763101119821233 30.75632718047428 19.145165023543107 44-45 20.802110607908524 28.808888206580296 31.31714935261234 19.071851832898837 46-47 21.111686976342302 28.74134101108707 30.840692044251224 19.30627996831941 48-49 20.899988039635627 29.68856234714285 29.91785636910253 19.493593244118998 50-51 20.964914542032943 29.186993041139015 29.732336336354553 20.11575608047349 52-53 21.285584642339707 28.847091325796832 29.785318911329238 20.082005120534223 54-55 21.976719814953153 28.925584439752328 29.449183939007007 19.648511806287512 56-57 22.012652993307356 29.171778920193503 29.12885304720633 19.68671503929281 58-59 21.620154715565175 29.23263808610554 29.721004097195635 19.426203101133645 60-61 20.822978009545874 29.507618682573554 29.630527857676213 20.03887545020435 62-63 21.161103146881963 29.214261803104424 29.38972954816582 20.23490550184779 64-65 20.761686867875458 29.21449833959553 29.64612014022877 20.377694652300242 66-67 21.157486752861885 28.384488650520932 29.767534398030904 20.69049019858628 68-69 21.22075750823807 28.709177223823858 30.176758490440204 19.89330677749787 70-71 20.500882388881994 30.265834451910045 29.46527139023376 19.768011768974205 72-73 20.321053282726997 30.54644194806226 28.99206479874395 20.140439970466797 74-75 20.946124253567557 29.753027738936527 29.045852799478045 20.25499520801787 76-77 20.799013732640002 29.455548435965945 29.298957462199336 20.44648036919472 78-79 20.707977813598152 29.58020820781677 29.498665072180074 20.213148906405003 80-81 19.989897499024707 30.034932447577173 29.47190559277258 20.50326446062554 82-83 19.42553590684858 29.72643213299549 29.643973262934065 21.20405869722187 84-85 19.667430334208937 29.323184874569076 30.045185189536927 20.96419960168506 86-87 19.92305839378569 29.393387888756667 30.61554990657578 20.06800381088186 88-89 20.12309571333289 30.035592867931488 29.895166030238112 19.94614538849751 90-91 20.414787953851977 29.663853634927932 29.424953753060862 20.496404658159225 92-93 19.835952735380996 30.212350512389563 29.681346344801636 20.270350407427806 94-95 19.4725418467515 30.628252610614336 29.77837723955053 20.12082830308363 96-97 19.829423175816686 31.196512368638757 29.275325136965098 19.698739318579467 98-99 20.480475586310405 30.318380929549892 28.70042856966887 20.500714914470837 100-101 20.436340782636435 30.106460221790392 28.141160291518037 21.31603870405514 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 239.0 1 237.0 2 231.5 3 250.0 4 256.5 5 247.5 6 285.5 7 368.0 8 482.0 9 598.0 10 701.0 11 764.0 12 793.5 13 781.5 14 716.0 15 656.0 16 598.5 17 575.0 18 616.5 19 654.0 20 762.0 21 952.5 22 1182.5 23 1602.5 24 2177.0 25 3255.5 26 5290.0 27 8090.5 28 10966.0 29 14019.0 30 17534.0 31 20658.0 32 26379.0 33 37375.0 34 54205.5 35 78796.0 36 113209.0 37 151257.5 38 185172.5 39 204611.0 40 203134.0 41 185889.5 42 166936.5 43 158660.0 44 146808.5 45 131978.5 46 123946.5 47 110461.5 48 91964.5 49 77069.0 50 63783.5 51 52403.5 52 42860.0 53 34215.0 54 28004.0 55 22091.0 56 15843.5 57 10797.5 58 7477.5 59 5509.5 60 4105.5 61 2956.0 62 2149.5 63 1634.0 64 1251.5 65 937.0 66 716.5 67 556.5 68 438.5 69 324.0 70 239.5 71 184.5 72 128.0 73 95.0 74 80.5 75 65.5 76 60.5 77 53.0 78 38.5 79 29.5 80 24.5 81 19.5 82 14.0 83 15.0 84 18.0 85 16.5 86 17.0 87 14.5 88 9.5 89 7.5 90 6.0 91 6.0 92 7.0 93 5.0 94 5.0 95 4.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22247037826395177 2 0.20525833573545355 3 0.18089667554127145 4 0.16353331835318202 5 0.1756763197853753 6 0.1352753056745267 7 0.12139218565703475 8 0.11681491720440115 9 0.10365054181996733 10-11 0.09018353711635115 12-13 0.11689057453419674 14-15 0.11318336537421249 16-17 0.10387751380935412 18-19 0.0975979554363196 20-21 0.10153213658569062 22-23 0.08870821918533701 24-25 0.4048991147335841 26-27 0.19273704765428232 28-29 0.09061856676267581 30-31 0.1733498568941607 32-33 0.20488004908647559 34-35 0.2040099897938262 36-37 0.20972211819339373 38-39 0.2109326354701233 40-41 0.2062418810227963 42-43 0.22279192191558306 44-45 0.2041045614560707 46-47 0.22398352485986373 48-49 0.21254035372827973 50-51 0.21600167656642827 52-53 0.20775502761870823 54-55 0.19031601310082324 56-57 0.19780608875058728 58-59 0.19322882029795369 60-61 0.17240414027171574 62-63 0.16139599878645644 64-65 0.16576520958215213 66-67 0.17857021265005685 68-69 0.17136385198702628 70-71 0.16476274996236048 72-73 0.16775121448928654 74-75 0.15863450624891715 76-77 0.168602359449487 78-79 0.1665974402099037 80-81 0.17202585362273776 82-83 0.1972008301122225 84-85 0.19419345125284757 86-87 0.19911117768956133 88-89 0.2069038826585078 90-91 0.18706274791961258 92-93 0.16801601514357115 94-95 0.1688671601037716 96-97 0.17730295237598062 98-99 0.19181024536428626 100-101 0.20338581682301254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2643498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.586593250008335 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6959800166056 42.60848910727676 2 5.455649411235372 5.30142837716681 3 2.0377695401244194 2.9702483934984514 4 1.0612092248721223 2.0624216384807372 5 0.6180571854796898 1.5014646538073324 6 0.49652649602868837 1.4474718540238658 7 0.3463819683238086 1.1780663862860312 8 0.24229470874238854 0.9417819568236537 9 0.2313336185215882 1.0115741185344902 >10 1.4901871500371269 14.755429027530337 >50 0.17168207455228554 5.7626288181985466 >100 0.13629008123911246 13.666268148241878 >500 0.014104835544193938 4.491556319291966 >1k 0.0023768619409070173 1.8415522452568882 >5k 1.5682675273445858E-4 0.45961895558236915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6237 0.23593738296756797 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5827 0.2204276303594707 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2924 0.1106110161611622 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2839 0.10739557964484936 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2718 0.10281831119221578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.430873032625711E-4 0.0 0.0 2 6.809159681603693E-4 0.0 0.0 3 6.809159681603693E-4 0.0 0.0 4 6.809159681603693E-4 0.0 0.0 5 9.078879575471591E-4 0.0 0.0 6 0.0010592026171383523 0.0 0.0 7 0.0012105172767295455 0.0 0.0 8 0.0012861746065251421 0.0 0.0 9 0.0015509752608097302 0.0 0.0 10-11 0.002421034553459091 0.0 0.0 12-13 0.003310008178557351 0.0 0.0 14-15 0.004539439787735796 0.0 0.0 16-17 0.0062606440405856174 0.0 0.0 18-19 0.008606021264249112 0.0 0.0 20-21 0.010573111838934623 0.0 0.0 22-23 0.012767174403006926 0.0 0.0 24-25 0.015396266613403906 0.0 0.0 26-27 0.017817301166862996 0.0 0.0 28-29 0.020805765693789065 0.0 0.0 30-31 0.03164367818700828 0.0 0.0 32-33 0.03521848701985021 0.0 0.0 34-35 0.03900135350963004 0.0 0.0 36-37 0.042765305666960976 0.0 0.0 38-39 0.04770194643612365 0.0 0.0 40-41 0.0533384175058956 0.0 0.0 42-43 0.05891814557832085 0.0 0.0 44-45 0.06508421795666197 0.0 0.0 46-47 0.07079634635622951 0.0 0.0 48-49 0.07783247802721999 0.0 0.0 50-51 0.08492535269555718 0.0 0.0 52-53 0.09245325701021903 0.0 0.0 54-55 0.10098362094467256 0.0 0.0 56-57 0.11045970150157103 0.0 0.0 58-59 0.12143001432193254 0.0 0.0 60-61 0.13224901248270285 0.0 0.0 62-63 0.1445054999095895 0.0 0.0 64-65 0.15853993458667265 0.0 0.0 66-67 0.17253654059885803 0.0 0.0 68-69 0.18980532612470297 0.0 0.0 70-71 0.20945731753910918 0.0 0.0 72-73 0.2327786894486018 0.0 0.0 74-75 0.25513543040320064 0.0 0.0 76-77 0.28138852384227264 0.0 0.0 78-79 0.31061116747582185 0.0 0.0 80-81 0.34390039258588434 0.0 0.0 82-83 0.3805185402069531 0.0 0.0 84-85 0.4241349908341145 0.0 0.0 86-87 0.47310419754431443 0.0 0.0 88-89 0.5286745062791801 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 555 0.0 68.44679 2 GAGCGGC 510 0.0 60.481083 9 GTCGCGC 25 0.00502339 56.996002 1 AGAGCGG 570 0.0 54.114655 8 CGAGCGG 45 1.5171407E-5 52.77407 1 CGGGAGA 520 0.0 52.044907 4 CGGAAAG 255 0.0 50.272476 5 GCCGAAC 50 2.8291726E-5 47.49667 1 TCGGGAA 240 0.0 47.480465 3 TTCGGAG 170 0.0 47.48046 3 ATTCGGA 165 0.0 46.046024 2 AAGCGGC 200 0.0 45.08167 9 CTCGACC 45 9.630674E-4 42.219257 1 TCGGAGA 305 0.0 40.47438 4 GAGAGCG 760 0.0 39.96159 7 GATCGGG 1075 0.0 39.76465 1 TTGTGTT 34150 0.0 39.61069 1 CGAGGCG 60 8.2971084E-5 39.580555 1 TCTCGGG 360 0.0 35.624187 48-49 GACGGCG 95 8.771276E-7 34.966393 7 >>END_MODULE