FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643498
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG62370.23593738296756797No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC58270.2204276303594707No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29240.1106110161611622No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA28390.10739557964484936No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27180.10281831119221578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5550.068.446792
GAGCGGC5100.060.4810839
GTCGCGC250.0050233956.9960021
AGAGCGG5700.054.1146558
CGAGCGG451.5171407E-552.774071
CGGGAGA5200.052.0449074
CGGAAAG2550.050.2724765
GCCGAAC502.8291726E-547.496671
TCGGGAA2400.047.4804653
TTCGGAG1700.047.480463
ATTCGGA1650.046.0460242
AAGCGGC2000.045.081679
CTCGACC459.630674E-442.2192571
TCGGAGA3050.040.474384
GAGAGCG7600.039.961597
GATCGGG10750.039.764651
TTGTGTT341500.039.610691
CGAGGCG608.2971084E-539.5805551
TCTCGGG3600.035.62418748-49
GACGGCG958.771276E-734.9663937
Produced by FastQC (version 0.11.2)