FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884870
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC90050.18434472155860854No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG75530.1546202867220622No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA70520.14436412842102248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC18250.074.358989
CGGGAGA24950.071.9292144
AGAGCGG20500.066.893398
AAGCGGC7050.063.938849
CGGAAAG10850.061.698335
GAGAGCG25150.061.5087057
TCGGGAG27150.061.3811043
GTTCGGA3350.058.119992
TCGGGAA9650.052.6446083
ATTCGGA4850.051.8944172
TTGTGTT669900.051.241721
GATCGGG42250.051.0393071
GATCCGG6100.049.8341
TCGGAGA6700.049.6029244
TCGGAAA10350.046.789014
ATCGGGA44350.046.2569122
TTCGGAG5200.043.826353
ATCCGGA6650.042.8465732
GGATCGG9400.042.445051
CCGGATC11650.042.38493760-61
Produced by FastQC (version 0.11.2)