FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654147
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59830.22542082258443108No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG58440.22018373511339048No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC57410.21630301562046111No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT53580.20187276740888882No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52530.19791669413939772No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT48060.18107512507784987No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA42940.16178455827804564No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG40780.1536463504093782No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA39030.14705289496022642No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT38930.14667612607741773No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG33210.12512494598076143No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA30570.11517824747461237No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA30510.11495218614492717No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT28730.10824570003093271No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA27650.10417659609659902No Hit
TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA26640.1003712303802314No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.10033355349195051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA6050.062.7860454
GAGCGGC4500.060.112049
AGAGCGG4900.056.173448
CGGAAAG2500.049.381235
TTGTGTT365050.048.042591
TCGGGAA1350.045.7250863
GAACCGG857.225026E-944.70831
CGCAGCC751.3631325E-744.3357281
ATCGGGA8600.043.625372
GGAGAGC6700.043.2135246
CGACCGC459.636425E-442.2141462
CGACTGG608.291047E-539.5854721
AAGCGGC2300.039.2035039
ACGGCGA500.001615767638.0020561
ACCGGGT756.9231337E-638.0020521
TGTGTTT624350.037.8894461
CCGTGTA2650.037.64069738-39
GGCGCCG4300.036.9962956-57
TGTACCG651.3301494E-436.5314672
GATCGGG13150.035.4011541
Produced by FastQC (version 0.11.2)