##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2654147 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94674447195276 33.0 33.0 33.0 33.0 33.0 2 31.91274183381704 33.0 33.0 33.0 33.0 33.0 3 31.893303196846293 33.0 33.0 33.0 33.0 33.0 4 35.62131826157331 37.0 37.0 37.0 33.0 37.0 5 35.57868422510132 37.0 37.0 37.0 33.0 37.0 6 35.634586554550296 37.0 37.0 37.0 33.0 37.0 7 35.55368523295808 37.0 37.0 37.0 33.0 37.0 8 35.491680001145376 37.0 37.0 37.0 33.0 37.0 9 35.45468732515569 37.0 37.0 37.0 33.0 37.0 10-11 35.383193922567216 37.0 37.0 37.0 33.0 37.0 12-13 35.3852599347361 37.0 37.0 37.0 33.0 37.0 14-15 37.032092608284316 40.0 37.0 40.0 33.0 40.0 16-17 36.995347092681754 40.0 37.0 40.0 33.0 40.0 18-19 36.986308407183174 40.0 37.0 40.0 33.0 40.0 20-21 36.94399047980387 40.0 37.0 40.0 33.0 40.0 22-23 36.96928542390455 40.0 37.0 40.0 33.0 40.0 24-25 36.770843890711404 40.0 37.0 40.0 33.0 40.0 26-27 36.651981220331805 40.0 37.0 40.0 33.0 40.0 28-29 36.613692082616375 40.0 37.0 40.0 33.0 40.0 30-31 36.605364359999655 40.0 37.0 40.0 33.0 40.0 32-33 36.51750411714197 40.0 37.0 40.0 33.0 40.0 34-35 36.47643404830252 40.0 37.0 40.0 33.0 40.0 36-37 36.39267851403859 38.5 37.0 40.0 33.0 40.0 38-39 36.35219507434969 37.0 37.0 40.0 33.0 40.0 40-41 36.21181890076171 37.0 37.0 40.0 33.0 40.0 42-43 36.12218558354153 37.0 37.0 40.0 33.0 40.0 44-45 36.014272382049676 37.0 37.0 40.0 33.0 40.0 46-47 35.93285695931687 37.0 37.0 40.0 27.0 40.0 48-49 35.81861592443825 37.0 37.0 40.0 27.0 40.0 50-51 34.64824404978323 37.0 35.0 38.5 27.0 38.5 52-53 35.124554141123305 37.0 35.0 38.5 27.0 40.0 54-55 35.933398941354795 37.0 37.0 40.0 27.0 40.0 56-57 35.98203792028098 37.0 37.0 40.0 27.0 40.0 58-59 35.729058337763504 37.0 37.0 40.0 27.0 40.0 60-61 35.50702127651559 37.0 37.0 40.0 27.0 40.0 62-63 35.28662730436558 37.0 37.0 40.0 27.0 40.0 64-65 35.02860448196728 37.0 33.0 40.0 27.0 40.0 66-67 34.77728550830078 37.0 33.0 37.0 27.0 40.0 68-69 34.553238196678635 37.0 33.0 37.0 27.0 40.0 70-71 34.208140129389975 37.0 33.0 37.0 27.0 40.0 72-73 33.97314429080228 37.0 33.0 37.0 27.0 38.5 74-75 33.706532644951466 37.0 33.0 37.0 27.0 37.0 76-77 33.4435492834421 37.0 33.0 37.0 27.0 37.0 78-79 33.19526499474219 37.0 33.0 37.0 27.0 37.0 80-81 33.015060394168074 37.0 33.0 37.0 27.0 37.0 82-83 32.8513160725461 37.0 33.0 37.0 27.0 37.0 84-85 32.69660723388719 37.0 33.0 37.0 27.0 37.0 86-87 32.567533184861276 33.0 33.0 37.0 27.0 37.0 88-89 32.42411516769795 33.0 33.0 37.0 22.0 37.0 90-91 32.29966614509294 33.0 33.0 37.0 22.0 37.0 92-93 32.12935248123031 33.0 33.0 37.0 22.0 37.0 94-95 32.00059793221702 33.0 33.0 37.0 22.0 37.0 96-97 31.870370216871937 33.0 33.0 37.0 22.0 37.0 98-99 31.718540646015462 33.0 33.0 37.0 22.0 37.0 100-101 30.450854078541994 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.19391835413312108 2306 2 0.20974109838180155 2306 3 0.21221301523827307 2306 4 0.3530883539128524 2306 5 0.4157851702185127 2306 6 0.23203080929609854 2306 7 0.3839935777928929 2306 8 0.3251400217193563 2306 9 0.2629423718713255 2306 10-11 0.16410967861896353 2306 12-13 0.22528126558614048 2306 14-15 0.4400385868269936 2306 16-17 0.445683738916955 2306 18-19 0.4586724471838082 2306 20-21 0.35580118472940825 2306 22-23 0.4821571973705687 2306 24-25 0.4181701774658464 2306 26-27 0.4389670422983656 2306 28-29 0.4138110235648824 2306 30-31 0.4080937420722037 2306 32-33 0.5419615314102373 2306 34-35 0.580896233581683 2306 36-37 0.4907927106675487 2306 38-39 0.472704801332533 2306 40-41 0.5598574641535308 2306 42-43 0.5381822326789845 2306 44-45 0.5823874517264755 2306 46-47 0.5857258595717454 2306 48-49 0.5761480174220921 2306 50-51 0.5493396178388466 2306 52-53 0.44994552702127777 2306 54-55 0.45621598314370715 2306 56-57 0.3166877117156801 2306 58-59 0.4833052968173561 2306 60-61 0.4941454243610721 2306 62-63 0.46908367301972476 2306 64-65 0.4986622707259514 2306 66-67 0.48148662515850305 2306 68-69 0.444559501599457 2306 70-71 0.4803386424096203 2306 72-73 0.41493012040230326 2306 74-75 0.47710406971980035 2306 76-77 0.45856227437011654 2306 78-79 0.37063287703821857 2306 80-81 0.5547463667147454 2306 82-83 0.5345936357886742 2306 84-85 0.5821632929502627 2306 86-87 0.4409109258615729 2306 88-89 0.6335903836824599 2306 90-91 0.3743637212884394 2306 92-93 0.3997233174335548 2306 94-95 0.5024260189095742 2306 96-97 0.38720822217915085 2306 98-99 0.48421318591728735 2306 100-101 0.42185289615001054 2307 1 0.3161868434055215 2307 2 0.3176923426861542 2307 3 0.3104437691192494 2307 4 0.39128898827490133 2307 5 0.48781569103702793 2307 6 0.3533776324696092 2307 7 0.44568236554840723 2307 8 0.43560214466035063 2307 9 0.397844632268459 2307 10-11 0.44638728532992644 2307 12-13 0.4215283426588172 2307 14-15 0.6383503172330478 2307 16-17 0.7367733996093264 2307 18-19 0.7275484114328705 2307 20-21 0.6990399480651845 2307 22-23 0.5587651794840767 2307 24-25 0.6170237725699863 2307 26-27 0.5612258849459266 2307 28-29 0.5909073453895175 2307 30-31 0.6636251167548224 2307 32-33 0.7270052940128551 2307 34-35 0.5758386459875737 2307 36-37 0.45139617044333136 2307 38-39 0.43291026892925544 2307 40-41 0.6045276763682494 2307 42-43 0.6799677733254512 2307 44-45 0.6114527323660823 2307 46-47 0.48853280690558165 2307 48-49 0.438624906865158 2307 50-51 0.3512175125153476 2307 52-53 0.4954067624727898 2307 54-55 0.5706624377610225 2307 56-57 0.4061083409402144 2307 58-59 0.41397059421453974 2307 60-61 0.45986159530146864 2307 62-63 0.38948882023824893 2307 64-65 0.5565690303089141 2307 66-67 0.6758699743122989 2307 68-69 0.5795968147443347 2307 70-71 0.4996536658977817 2307 72-73 0.43766583705833284 2307 74-75 0.4176541257804729 2307 76-77 0.4448811554057386 2307 78-79 0.3186604454383044 2307 80-81 0.45727549125322753 2307 82-83 0.4776920545139376 2307 84-85 0.5603486911574223 2307 86-87 0.6672086173966818 2307 88-89 0.4687055864324634 2307 90-91 0.472039380814401 2307 92-93 0.6248129759209817 2307 94-95 0.5091563312507432 2307 96-97 0.36336507299161624 2307 98-99 0.3372862698495389 2307 100-101 0.17539293184803384 2201 1 0.3240310315553536 2201 2 0.32117266109475295 2201 3 0.33952765746360214 2201 4 0.4095886956834818 2201 5 0.4636299297094908 2201 6 0.39803435578551216 2201 7 0.39209570542584515 2201 8 0.42562553935195524 2201 9 0.4330252675418649 2201 10-11 0.3746225274739672 2201 12-13 0.4675426765800452 2201 14-15 0.5721021723267441 2201 16-17 0.5653307010687953 2201 18-19 0.5069792878623858 2201 20-21 0.651864617983513 2201 22-23 0.4882657933580603 2201 24-25 0.46839429661788046 2201 26-27 0.5769285825446602 2201 28-29 0.6272081975310897 2201 30-31 0.526266867273911 2201 32-33 0.47138746103415485 2201 34-35 0.43598327168950135 2201 36-37 0.45892201310613956 2201 38-39 0.4303926330990109 2201 40-41 0.5172233380826654 2201 42-43 0.5364392285490567 2201 44-45 0.5856564317275499 2201 46-47 0.5831066608739661 2201 48-49 0.6218451747559826 2201 50-51 0.6034819769060462 2201 52-53 0.6130098721851098 2201 54-55 0.7163959771268722 2201 56-57 0.731070389497674 2201 58-59 0.601458609229077 2201 60-61 0.69995768879091 2201 62-63 0.7057714333558707 2201 64-65 0.631112727019719 2201 66-67 0.5838234655294627 2201 68-69 0.6598852942544369 2201 70-71 0.7665055870636337 2201 72-73 0.7656382712276937 2201 74-75 0.798464264677655 2201 76-77 0.8500205113550692 2201 78-79 0.7348085111648146 2201 80-81 0.6692860608574023 2201 82-83 0.7219294307742743 2201 84-85 0.7124923468947557 2201 86-87 0.6954178141951246 2201 88-89 0.646881116041385 2201 90-91 0.7689003705181179 2201 92-93 0.7473723474149949 2201 94-95 0.6939887162227976 2201 96-97 0.5596146888652491 2201 98-99 0.579898664132859 2201 100-101 0.5718853786978109 1101 1 0.1355280512843855 1101 2 0.16324094568362923 1101 3 0.16457461505092041 1101 4 0.22173628151651315 1101 5 0.2957406741303785 1101 6 0.26048805143530984 1101 7 0.30122246733699853 1101 8 0.3058354392446745 1101 9 0.287705847946242 1101 10-11 0.29054283928116575 1101 12-13 0.251001017239318 1101 14-15 0.41838485247328094 1101 16-17 0.4409254371737674 1101 18-19 0.5074476443596083 1101 20-21 0.39434797814698896 1101 22-23 0.3748193514854776 1101 24-25 0.4437694056939634 1101 26-27 0.3843555576027242 1101 28-29 0.402486964293729 1101 30-31 0.4252990543861088 1101 32-33 0.3470932184164326 1101 34-35 0.37862324617783116 1101 36-37 0.4018179404136788 1101 38-39 0.3349833164628464 1101 40-41 0.43117128025219387 1101 42-43 0.3974535960494521 1101 44-45 0.4042732134529885 1101 46-47 0.32026866437600887 1101 48-49 0.32560487082709955 1101 50-51 0.32753801152795603 1101 52-53 0.42850725795918976 1101 54-55 0.4531129664713873 1101 56-57 0.43906329435544933 1101 58-59 0.5301691296099662 1101 60-61 0.4900121565727815 1101 62-63 0.45986824623229694 1101 64-65 0.3885366942837791 1101 66-67 0.41994491165824144 1101 68-69 0.29947817597892623 1101 70-71 0.44371389433523234 1101 72-73 0.39483185029585854 1101 74-75 0.2404584344347569 1101 76-77 0.3984147558076643 1101 78-79 0.46218624201532776 1101 80-81 0.4430294590979287 1101 82-83 0.4170594290253362 1101 84-85 0.3123857153708869 1101 86-87 0.35969040069591074 1101 88-89 0.526977788166981 1101 90-91 0.4029152459756986 1101 92-93 0.27636666883924477 1101 94-95 0.36891388718511564 1101 96-97 0.30555979929541977 1101 98-99 0.3796232477508177 1101 100-101 0.3482604225082433 2304 1 0.22680580387005378 2304 2 0.21257991336301885 2304 3 0.14791285650253982 2304 4 0.18206913011887593 2304 5 0.32521805074549803 2304 6 0.24861518299199048 2304 7 0.2603074377232346 2304 8 0.2606653316830503 2304 9 0.24030059200307363 2304 10-11 0.22383619099606022 2304 12-13 0.297052631532452 2304 14-15 0.3507989777771101 2304 16-17 0.2849855064607638 2304 18-19 0.26801942710978466 2304 20-21 0.3254242068705153 2304 22-23 0.23993285932196784 2304 24-25 0.2535676603149781 2304 26-27 0.37816872247901756 2304 28-29 0.24987445545477271 2304 30-31 0.3099064787619952 2304 32-33 0.1900116072679765 2304 34-35 0.35938700584856065 2304 36-37 0.3424033225606422 2304 38-39 0.24093747203195193 2304 40-41 0.2380395402764819 2304 42-43 0.2838157813000848 2304 44-45 0.2901828093888881 2304 46-47 0.3410973284817729 2304 48-49 0.3675299738107256 2304 50-51 0.25909723383878713 2304 52-53 0.2910954737843596 2304 54-55 0.30385795801900173 2304 56-57 0.30171970262054515 2304 58-59 0.38283328227763747 2304 60-61 0.3997515354916814 2304 62-63 0.4547586996381838 2304 64-65 0.4630549603020526 2304 66-67 0.42480709777756687 2304 68-69 0.5377299650531882 2304 70-71 0.5480877444022099 2304 72-73 0.6803108131453186 2304 74-75 0.5905523402023647 2304 76-77 0.5700602306963418 2304 78-79 0.5535970132935404 2304 80-81 0.44095512760101485 2304 82-83 0.5492384207154188 2304 84-85 0.6000632328880968 2304 86-87 0.4607967687404937 2304 88-89 0.43430610827010696 2304 90-91 0.43580897696217136 2304 92-93 0.4457050023723781 2304 94-95 0.42518568053335315 2304 96-97 0.4550972417227577 2304 98-99 0.4343525671989461 2304 100-101 0.46478881488667767 2202 1 0.3177831721090705 2202 2 0.31317287223467716 2202 3 0.2987447129864442 2202 4 0.44566150131158366 2202 5 0.5654777313787207 2202 6 0.39837766275226727 2202 7 0.48133976312596616 2202 8 0.41750176716539045 2202 9 0.5210102602399402 2202 10-11 0.40799644522841305 2202 12-13 0.4632548175756881 2202 14-15 0.6230255616789933 2202 16-17 0.6132376742920727 2202 18-19 0.6045119173687041 2202 20-21 0.5618683743100377 2202 22-23 0.5559910716760825 2202 24-25 0.6334294027218235 2202 26-27 0.668417843256222 2202 28-29 0.5308691632834552 2202 30-31 0.6904540379048356 2202 32-33 0.5627685943726988 2202 34-35 0.6020201671633671 2202 36-37 0.4444036883295652 2202 38-39 0.482991945584871 2202 40-41 0.48195212762485795 2202 42-43 0.5701617726869088 2202 44-45 0.5485329390149403 2202 46-47 0.4999489364876766 2202 48-49 0.4941881772884358 2202 50-51 0.44900151410043065 2202 52-53 0.4656975279059097 2202 54-55 0.47301384291963444 2202 56-57 0.6127902960232419 2202 58-59 0.7021453459183675 2202 60-61 0.6959813225498905 2202 62-63 0.5749993036619472 2202 64-65 0.6192329351971395 2202 66-67 0.7145303300729751 2202 68-69 0.5792485641735041 2202 70-71 0.5245455784379942 2202 72-73 0.4534456977061936 2202 74-75 0.5481767092892298 2202 76-77 0.6187719665224165 2202 78-79 0.47774606489603855 2202 80-81 0.5694102283175511 2202 82-83 0.5656783183001224 2202 84-85 0.5574004580640164 2202 86-87 0.6371065734902928 2202 88-89 0.4953625453440793 2202 90-91 0.41702811999159195 2202 92-93 0.6570222602379445 2202 94-95 0.650062405692438 2202 96-97 0.4150958566981622 2202 98-99 0.45165867905996393 2202 100-101 0.3959008672023465 1102 1 0.08963810621848367 1102 2 0.022202323791880474 1102 3 0.09639993805413738 1102 4 0.08490053295027877 1102 5 0.035179163932419044 1102 6 0.06614815375076688 1102 7 0.04666176440014169 1102 8 0.06460348642765013 1102 9 0.04016324267217186 1102 10-11 0.11331409055460284 1102 12-13 0.16089689053800527 1102 14-15 0.009271390281774927 1102 16-17 0.10587350275198304 1102 18-19 0.12392198865751425 1102 20-21 -0.013538892173237116 1102 22-23 0.20290475979386713 1102 24-25 0.2064087502528409 1102 26-27 0.1994416082680317 1102 28-29 0.1384761908307084 1102 30-31 0.07482202795173976 1102 32-33 0.12175642812569265 1102 34-35 0.25836691894137687 1102 36-37 0.30381317711565714 1102 38-39 0.2984460174437231 1102 40-41 0.3061943886732621 1102 42-43 0.2723443444254201 1102 44-45 0.29535243754238394 1102 46-47 0.2487238490115189 1102 48-49 0.17588268135697405 1102 50-51 0.3300025285344006 1102 52-53 0.30719423383472844 1102 54-55 0.32845974874982176 1102 56-57 0.4063205592375354 1102 58-59 0.44900486861050837 1102 60-61 0.49558729060162676 1102 62-63 0.3272997314534152 1102 64-65 0.45908324665291644 1102 66-67 0.528551683376989 1102 68-69 0.4531580659651624 1102 70-71 0.45080238300393916 1102 72-73 0.56060412489024 1102 74-75 0.6141819606061745 1102 76-77 0.5564714691237072 1102 78-79 0.6118000896417755 1102 80-81 0.506574029913466 1102 82-83 0.5050522414485883 1102 84-85 0.42607155688028797 1102 86-87 0.3429185218299935 1102 88-89 0.4918328170064967 1102 90-91 0.45263167523648207 1102 92-93 0.447126931213667 1102 94-95 0.4410137205549347 1102 96-97 0.2671457533680375 1102 98-99 0.3004949192284023 1102 100-101 0.5702148053933094 2305 1 0.2025377261603616 2305 2 0.17644308366644523 2305 3 0.10746935757426357 2305 4 0.12757096998691253 2305 5 0.1773716582375897 2305 6 0.291089392776513 2305 7 0.24957896731734763 2305 8 0.2894843028577654 2305 9 0.1522350730538662 2305 10-11 0.1949571017750813 2305 12-13 0.2145249652928385 2305 14-15 0.2585025127523366 2305 16-17 0.22400269834596997 2305 18-19 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100-101 -0.038285404661301925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 358.0 3 172.0 4 118.0 5 132.0 6 381.0 7 1576.0 8 4841.0 9 10303.0 10 11464.0 11 10513.0 12 7639.0 13 5950.0 14 5645.0 15 5884.0 16 6324.0 17 6455.0 18 6905.0 19 7739.0 20 8618.0 21 9938.0 22 11232.0 23 12402.0 24 13973.0 25 15932.0 26 18944.0 27 22360.0 28 27525.0 29 34104.0 30 42729.0 31 55957.0 32 77179.0 33 112617.0 34 172485.0 35 294537.0 36 560621.0 37 870843.0 38 199639.0 39 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.6333032635132 23.16967119425771 28.178203143850673 13.018822398378418 2 32.688884349924926 20.356775152519887 35.48791072956966 11.46642976798553 3 40.317813203591065 16.93264964491357 27.779931341779413 14.969605809715949 4 31.614713708689823 15.968368945441563 37.35137091426866 15.065546431599957 5 30.98543387193749 15.244708158473493 38.72050720455874 15.049350765030272 6 23.10202883613084 16.088458870013316 45.88786904863857 14.921643245217275 7 33.86391499024893 16.020166051173334 33.56561133765122 16.55030762092652 8 30.546685043902432 27.02985093768412 26.588412534240426 15.835051484173022 9 30.582747083775384 27.577232006981816 24.249969545069337 17.590051364173462 10-11 25.19567545553276 27.456539610232895 29.66870173162785 17.679083202606492 12-13 26.01857536091253 24.533131152534285 29.375153828091626 20.073139658461557 14-15 24.083144801073633 25.67698105976881 30.845958519633882 19.393915619523668 16-17 22.459631864402816 27.606190821106182 31.965498767368615 17.968678547122387 18-19 21.509453387944067 28.144125722601938 31.77766535155644 18.56875553789756 20-21 21.643090217068274 28.278539522295926 31.385347099546074 18.69302316108973 22-23 21.711902231748585 27.345934229371355 32.111393600324604 18.830769938555452 24-25 22.081434885835904 27.368248626384016 31.713694697865012 18.836621789915064 26-27 21.733506505174677 27.881792664532913 31.832047222232184 18.55265360806023 28-29 21.0153771834373 28.405864270566628 31.169262150497772 19.409496395498298 30-31 20.785762218526823 28.76940618416315 30.60489322305515 19.83993837425487 32-33 19.99441242179042 28.902276843735635 31.88215495524746 19.22115577922648 34-35 20.283534063464725 29.13201978142873 32.089059468495854 18.495386686610686 36-37 20.264563259377674 30.133185883893383 31.50884158617892 18.093409270550026 38-39 20.73354286774696 29.874001843636027 30.353071590882703 19.039383697734305 40-41 21.181049773359316 29.010158244503124 29.891028985693286 19.917762996444274 42-43 20.879574631661864 29.28821567180692 30.958981907269944 18.873227789261268 44-45 20.169716514938777 29.445888962593504 31.87131400896512 18.513080513502604 46-47 20.484127653942394 29.143478803484545 31.53891133489779 18.83348220767527 48-49 20.78213861958103 30.24758283674862 29.785209216889136 19.185069326781214 50-51 20.419583667338642 29.7829906309859 29.90572460241254 19.891701099262917 52-53 20.401752155742813 29.437975884072593 30.13796225434877 20.022309705835823 54-55 21.498333771218558 29.214645297867648 29.818492799052958 19.468528131860833 56-57 21.844029410121394 29.38596027610118 28.943717429679822 19.826292884097604 58-59 21.133763958479694 29.272443325203273 30.124683986550483 19.469108729766553 60-61 20.155361469042255 29.605070722983374 30.27659655573391 19.962971252240465 62-63 20.87624143952738 29.664977607624444 29.70035633453747 19.75842461831071 64-65 20.656154383938908 29.74113668892342 29.630409575215843 19.972299351921826 66-67 21.139732802703072 29.285264592961198 29.447129720075427 20.1278728842603 68-69 21.95671454774647 29.209981053886974 29.375401000898243 19.457903397468314 70-71 21.13080496480599 30.612403938921695 29.19190666958763 19.064884426684692 72-73 20.24684411122798 31.251403445408943 28.946526229663487 19.55522621369959 74-75 21.176819757210357 30.250289670959074 28.706283553198986 19.866607018631584 76-77 21.52730615810178 29.37470951949547 29.130644568101836 19.9673397543009 78-79 21.451897044027483 29.345799647816364 29.6997759037254 19.50252740443075 80-81 19.806590987832966 30.095319400758903 29.978227050087973 20.119862561320158 82-83 19.102355165815197 29.840525069810862 30.24117544243119 20.81594432194275 84-85 19.06482248025933 29.103562021960965 30.75990204505671 21.071713452723 86-87 19.16862945553703 29.168942797052477 31.882801212744923 19.779626534665574 88-89 19.5268116351036 29.80673664713312 31.223171894499608 19.443279823263676 90-91 19.85486545545688 29.62463720138593 30.281043652095335 20.239453691061858 92-93 19.100187095416775 30.60146977932553 30.40393928786748 19.89440383739021 94-95 18.790027004989817 30.87066042116915 30.758326758678496 19.580985815162535 96-97 19.45519454062234 31.689689915999846 29.73146032237896 19.12365522099885 98-99 20.12799566943015 30.569247971177855 28.992564949110523 20.31019141028148 100-101 19.59728069804528 30.54353948703699 28.637936580327157 21.221243234590574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 856.0 1 632.5 2 403.5 3 526.0 4 584.5 5 509.5 6 564.5 7 757.0 8 935.0 9 1008.5 10 1067.5 11 1091.5 12 1093.0 13 1080.5 14 1023.0 15 946.0 16 902.0 17 903.5 18 927.0 19 1015.0 20 1136.5 21 1328.5 22 1612.0 23 2007.0 24 2574.5 25 3415.5 26 4810.5 27 6976.5 28 9225.5 29 11138.5 30 13550.5 31 17172.5 32 24536.5 33 35936.5 34 52147.5 35 79057.5 36 125454.0 37 185339.5 38 234902.0 39 242605.0 40 218131.0 41 189263.0 42 163994.5 43 153245.5 44 137862.5 45 118453.0 46 113687.0 47 101854.0 48 77307.0 49 59567.5 50 48608.0 51 41028.0 52 35254.5 53 29048.5 54 23278.5 55 17287.0 56 12497.5 57 9573.5 58 7750.0 59 6073.5 60 4601.0 61 3586.5 62 2755.5 63 2041.0 64 1565.0 65 1144.5 66 803.5 67 614.5 68 451.0 69 329.5 70 243.0 71 204.5 72 156.0 73 104.5 74 78.0 75 57.5 76 44.5 77 38.5 78 32.0 79 31.5 80 30.0 81 26.5 82 19.5 83 16.0 84 14.0 85 8.0 86 7.5 87 10.0 88 8.5 89 6.0 90 6.0 91 5.5 92 3.0 93 1.5 94 3.5 95 4.5 96 2.5 97 1.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22101262665556956 2 0.20631864022603116 3 0.18054764864191772 4 0.16374375646865078 5 0.17497146917634931 6 0.1323965854189689 7 0.11894593630269915 8 0.11457541726211848 9 0.1012754756989722 10-11 0.08692058126396164 12-13 0.11508405525391019 14-15 0.11171197375277254 16-17 0.1019348212438874 18-19 0.09537904268301642 20-21 0.09912789306696275 22-23 0.08759876525301727 24-25 0.4053656410138549 26-27 0.19367804420780008 28-29 0.09017963210025669 30-31 0.17350207053339547 32-33 0.20400151159675783 34-35 0.20345519671668527 36-37 0.20931395284436016 38-39 0.2101051674982584 40-41 0.2057534869018182 42-43 0.2223313177453999 44-45 0.2048115646947965 46-47 0.2238195548324942 48-49 0.21247881145995307 50-51 0.21611463117905677 52-53 0.20812713086351284 54-55 0.19030596270666245 56-57 0.19829346302220638 58-59 0.19328243688085098 60-61 0.17271085587949725 62-63 0.16010793674954704 64-65 0.1653827011088685 66-67 0.17779723579741438 68-69 0.1705632732474878 70-71 0.16334814914170165 72-73 0.16645649242487323 74-75 0.1566416630277072 76-77 0.16737957618775448 78-79 0.16551457021785154 80-81 0.17063862702404953 82-83 0.19607052661363517 84-85 0.19494021996520916 86-87 0.19893397012298114 88-89 0.2055462640162734 90-91 0.1865005969902948 92-93 0.16688977664010318 94-95 0.16771866818228232 96-97 0.17661041381656706 98-99 0.19113485424884152 100-101 0.20266398206278702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2654147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.133260190526386 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2066622713923 36.321677316374064 2 6.675736340636168 5.625410724067524 3 1.9889401648833442 2.5140160041125523 4 1.0336278180847314 1.7420043919812025 5 0.7834830902171923 1.650534844749931 6 0.45410746402813784 1.147981676181458 7 0.3505858372054447 1.033992700866337 8 0.2916364203014253 0.9830074542074931 9 0.24015228894358476 0.9106558987869476 >10 1.5866960777188404 13.232669656438409 >50 0.17704804244402195 5.171911191083925 >100 0.18004628035718984 17.048816180357758 >500 0.0246009037398542 6.935081338918662 >1k 0.006229420214577657 4.619441594673185 >5k 4.475798333483727E-4 1.0627990272004926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5983 0.22542082258443108 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5844 0.22018373511339048 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5741 0.21630301562046111 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5358 0.20187276740888882 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5253 0.19791669413939772 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4806 0.18107512507784987 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 4294 0.16178455827804564 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 4078 0.1536463504093782 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3903 0.14705289496022642 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3893 0.14667612607741773 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3321 0.12512494598076143 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3057 0.11517824747461237 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 3051 0.11495218614492717 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2873 0.10824570003093271 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2765 0.10417659609659902 No Hit TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA 2664 0.1003712303802314 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2663 0.10033355349195051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.767688828086764E-4 0.0 0.0 2 4.1444577108954404E-4 0.0 0.0 3 4.521226593704117E-4 0.0 0.0 4 4.897995476512793E-4 0.0 0.0 5 4.897995476512793E-4 0.0 0.0 6 5.27476435932147E-4 0.0 0.0 7 5.651533242130146E-4 0.0 0.0 8 7.535377656173528E-4 0.0 0.0 9 9.042453187408234E-4 0.0 0.0 10-11 0.0014317217546729703 0.0 0.0 12-13 0.0022417748527116246 0.0 0.0 14-15 0.003164858615592882 0.0 0.0 16-17 0.004728449479248889 0.0 0.0 18-19 0.006386232563607065 0.0 0.0 20-21 0.007252800994067021 0.0 0.0 22-23 0.008157046312807843 0.0 0.0 24-25 0.00956992962334038 0.0 0.0 26-27 0.01109584359871552 0.0 0.0 28-29 0.012358019356124586 0.0 0.0 30-31 0.018857282584574253 0.0 0.0 32-33 0.021230926546268916 0.0 0.0 34-35 0.023265478513435765 0.0 0.0 36-37 0.025224676704040883 0.0 0.0 38-39 0.026957813564960798 0.0 0.0 40-41 0.02908655775282982 0.0 0.0 42-43 0.03149787860280535 0.0 0.0 44-45 0.034172937670746945 0.0 0.0 46-47 0.03675380451798638 0.0 0.0 48-49 0.0401070475749836 0.0 0.0 50-51 0.044119636176896 0.0 0.0 52-53 0.04871621654716186 0.0 0.0 54-55 0.05306789714360207 0.0 0.0 56-57 0.05854988438846831 3.767688828086764E-5 0.0 58-59 0.06491727850793494 3.767688828086764E-5 0.0 60-61 0.07141654173638462 3.767688828086764E-5 0.0 62-63 0.0791026269456816 3.767688828086764E-5 0.0 64-65 0.08797553413582593 3.767688828086764E-5 0.0 66-67 0.0981482939716602 3.767688828086764E-5 0.0 68-69 0.10935716823521832 3.767688828086764E-5 0.0 70-71 0.12160215692650031 3.767688828086764E-5 0.0 72-73 0.13644685090916214 3.767688828086764E-5 0.0 74-75 0.1537970579625017 3.767688828086764E-5 0.0 76-77 0.171787772116616 3.767688828086764E-5 0.0 78-79 0.19388526709334486 3.767688828086764E-5 0.0 80-81 0.2200895428926883 3.767688828086764E-5 0.0 82-83 0.25220909015212795 3.767688828086764E-5 0.0 84-85 0.2871920809209136 3.767688828086764E-5 0.0 86-87 0.3273556438283185 3.767688828086764E-5 0.0 88-89 0.3755632223836886 3.767688828086764E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 605 0.0 62.786045 4 GAGCGGC 450 0.0 60.11204 9 AGAGCGG 490 0.0 56.17344 8 CGGAAAG 250 0.0 49.38123 5 TTGTGTT 36505 0.0 48.04259 1 TCGGGAA 135 0.0 45.725086 3 GAACCGG 85 7.225026E-9 44.7083 1 CGCAGCC 75 1.3631325E-7 44.335728 1 ATCGGGA 860 0.0 43.62537 2 GGAGAGC 670 0.0 43.213524 6 CGACCGC 45 9.636425E-4 42.214146 2 CGACTGG 60 8.291047E-5 39.585472 1 AAGCGGC 230 0.0 39.203503 9 ACGGCGA 50 0.0016157676 38.002056 1 ACCGGGT 75 6.9231337E-6 38.002052 1 TGTGTTT 62435 0.0 37.889446 1 CCGTGTA 265 0.0 37.640697 38-39 GGCGCCG 430 0.0 36.99629 56-57 TGTACCG 65 1.3301494E-4 36.531467 2 GATCGGG 1315 0.0 35.401154 1 >>END_MODULE