##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34310-1.34200000035810.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5775383 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.978683664788985 33.0 33.0 33.0 33.0 33.0 2 31.95189583097779 33.0 33.0 33.0 33.0 33.0 3 31.918409913247313 33.0 33.0 33.0 33.0 33.0 4 35.64950341821486 37.0 37.0 37.0 33.0 37.0 5 35.596867255383756 37.0 37.0 37.0 33.0 37.0 6 35.63911847924198 37.0 37.0 37.0 33.0 37.0 7 35.56823192505155 37.0 37.0 37.0 33.0 37.0 8 35.512175556149266 37.0 37.0 37.0 33.0 37.0 9 35.454217495186036 37.0 37.0 37.0 33.0 37.0 10-11 35.37673891064887 37.0 37.0 37.0 33.0 37.0 12-13 35.37902152982755 37.0 37.0 37.0 33.0 37.0 14-15 37.054358992295406 40.0 37.0 40.0 33.0 40.0 16-17 36.9684518758323 40.0 37.0 40.0 33.0 40.0 18-19 36.97777186378808 40.0 37.0 40.0 33.0 40.0 20-21 36.94240684990069 40.0 37.0 40.0 33.0 40.0 22-23 36.97858696124569 40.0 37.0 40.0 33.0 40.0 24-25 36.77545515163237 40.0 37.0 40.0 33.0 40.0 26-27 36.652887089912475 40.0 37.0 40.0 33.0 40.0 28-29 36.629486044475314 40.0 37.0 40.0 33.0 40.0 30-31 36.63645727045289 40.0 37.0 40.0 33.0 40.0 32-33 36.57807984336277 40.0 37.0 40.0 33.0 40.0 34-35 36.51621624055063 40.0 37.0 40.0 33.0 40.0 36-37 36.410011595767756 40.0 37.0 40.0 33.0 40.0 38-39 36.32942897466714 37.0 37.0 40.0 33.0 40.0 40-41 36.217054609192154 37.0 37.0 40.0 33.0 40.0 42-43 36.09984108413243 37.0 37.0 40.0 33.0 40.0 44-45 35.9665820431303 37.0 37.0 40.0 30.0 40.0 46-47 35.841918882262874 37.0 37.0 40.0 27.0 40.0 48-49 35.70219827845184 37.0 37.0 40.0 27.0 40.0 50-51 34.48268097544353 37.0 35.0 38.5 27.0 38.5 52-53 34.93625331861108 37.0 35.0 38.5 27.0 40.0 54-55 35.71854922868319 37.0 37.0 40.0 27.0 40.0 56-57 35.719115684622125 37.0 37.0 40.0 27.0 40.0 58-59 35.49502613073453 37.0 37.0 40.0 27.0 40.0 60-61 35.26942620082512 37.0 35.0 40.0 27.0 40.0 62-63 35.0442695315618 37.0 33.0 40.0 27.0 40.0 64-65 34.777081277553364 37.0 33.0 37.0 27.0 40.0 66-67 34.47398683342732 37.0 33.0 37.0 27.0 40.0 68-69 34.23512743657001 37.0 33.0 37.0 27.0 40.0 70-71 33.92525404808651 37.0 33.0 37.0 27.0 37.0 72-73 33.68218047184057 37.0 33.0 37.0 27.0 37.0 74-75 33.41447173287035 37.0 33.0 37.0 27.0 37.0 76-77 33.156644416482855 37.0 33.0 37.0 27.0 37.0 78-79 32.966009267264184 37.0 33.0 37.0 27.0 37.0 80-81 32.82675789640271 37.0 33.0 37.0 27.0 37.0 82-83 32.5946842832761 35.0 33.0 37.0 24.5 37.0 84-85 32.43486371380045 33.0 33.0 37.0 22.0 37.0 86-87 32.27499111314349 33.0 33.0 37.0 22.0 37.0 88-89 32.13731807916462 33.0 33.0 37.0 22.0 37.0 90-91 32.01825532609699 33.0 33.0 37.0 22.0 37.0 92-93 31.801392392504532 33.0 33.0 37.0 22.0 37.0 94-95 31.627853252329757 33.0 33.0 37.0 22.0 37.0 96-97 31.48675412522425 33.0 33.0 37.0 22.0 37.0 98-99 31.322809673401746 33.0 33.0 37.0 18.5 37.0 100-101 29.943194330142262 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.27214969993755744 2306 2 0.27158563633981103 2306 3 0.24892991574314394 2306 4 0.33649017070089116 2306 5 0.31496879643621156 2306 6 0.33566788567397765 2306 7 0.36139086206506477 2306 8 0.3207349812826621 2306 9 0.39752365567400005 2306 10-11 0.3634235117182456 2306 12-13 0.3978247598402618 2306 14-15 0.553918491550462 2306 16-17 0.5170392628429212 2306 18-19 0.5411979547566261 2306 20-21 0.5775528560573022 2306 22-23 0.532091159345434 2306 24-25 0.4566981881454808 2306 26-27 0.5261872478329934 2306 28-29 0.48017183615458947 2306 30-31 0.43067158537645867 2306 32-33 0.46864703424583354 2306 34-35 0.3922250228187991 2306 36-37 0.3883374565134545 2306 38-39 0.4162542347725946 2306 40-41 0.33429380736225056 2306 42-43 0.35837166787244 2306 44-45 0.5536491890677127 2306 46-47 0.49115516898903167 2306 48-49 0.5341653359039427 2306 50-51 0.5072893029390002 2306 52-53 0.5402125197810435 2306 54-55 0.4730770913967248 2306 56-57 0.39655345945502773 2306 58-59 0.45223793257253675 2306 60-61 0.5412029457913192 2306 62-63 0.5829283696216621 2306 64-65 0.5074088895027202 2306 66-67 0.5323955670903686 2306 68-69 0.4462297470168224 2306 70-71 0.5013944149163407 2306 72-73 0.45590169803722347 2306 74-75 0.466140273829609 2306 76-77 0.3263582070570905 2306 78-79 0.18138595257007495 2306 80-81 0.2687618692940603 2306 82-83 0.41271356481896504 2306 84-85 0.3972414997393017 2306 86-87 0.2967318218177226 2306 88-89 0.23133762449656103 2306 90-91 0.17549339636103412 2306 92-93 0.21677480629709223 2306 94-95 0.21298940040725256 2306 96-97 0.17488707836418982 2306 98-99 0.22764039694045124 2306 100-101 0.24085725224980692 2307 1 0.11030598194339092 2307 2 0.082993158707108 2307 3 0.04361058850859578 2307 4 0.08762619180577502 2307 5 0.15730364078208936 2307 6 0.10916996865503137 2307 7 0.22470401564147124 2307 8 0.15564235713619468 2307 9 0.15602851674943707 2307 10-11 0.11899225757247223 2307 12-13 0.14365987652972478 2307 14-15 0.3287835294412318 2307 16-17 0.26979686025644867 2307 18-19 0.3206645836494175 2307 20-21 0.29624826040394936 2307 22-23 0.17770967845570596 2307 24-25 0.25202570208499964 2307 26-27 0.18096018773806577 2307 28-29 0.20988462716623246 2307 30-31 0.2671680300658039 2307 32-33 0.28234990063971566 2307 34-35 0.24903038170734249 2307 36-37 0.21662892875619377 2307 38-39 0.13462329861319233 2307 40-41 0.2685092113130594 2307 42-43 0.11169193224277052 2307 44-45 0.18600267429168582 2307 46-47 0.18179860924855262 2307 48-49 0.12647499415029273 2307 50-51 -0.0368154553552813 2307 52-53 0.10759933149355305 2307 54-55 0.15383403106613258 2307 56-57 0.08317382665828887 2307 58-59 0.06581150552757009 2307 60-61 0.09639919628159532 2307 62-63 0.07527559269812656 2307 64-65 0.05559408359736295 2307 66-67 0.17867562765668055 2307 68-69 0.11375183009107559 2307 70-71 0.12336371877139385 2307 72-73 0.17058746383187895 2307 74-75 0.07145818569453866 2307 76-77 -0.009978605803254936 2307 78-79 0.006663982081001052 2307 80-81 0.06364978439075486 2307 82-83 0.07541953633815979 2307 84-85 0.1564963053362689 2307 86-87 0.08666358431708687 2307 88-89 0.010815846762405101 2307 90-91 0.018232512844740256 2307 92-93 0.1265525880895595 2307 94-95 0.006951658023048424 2307 96-97 -0.006313717763955395 2307 98-99 -0.12872205482263155 2307 100-101 -0.1866829579118665 2201 1 0.30441191150303837 2201 2 0.21296045521180318 2201 3 0.2713011484854597 2201 4 0.32618749453529006 2201 5 0.33195347106354234 2201 6 0.3197941737784049 2201 7 0.37984050598782915 2201 8 0.44586102068557665 2201 9 0.38608078382925015 2201 10-11 0.4313236758878247 2201 12-13 0.39773938634617423 2201 14-15 0.4937286833518115 2201 16-17 0.506583252117089 2201 18-19 0.5426357949910425 2201 20-21 0.535211201742662 2201 22-23 0.47655260097709373 2201 24-25 0.5797107075981245 2201 26-27 0.5764401792664415 2201 28-29 0.5067209996604447 2201 30-31 0.5508144591922246 2201 32-33 0.49592857166855 2201 34-35 0.504561260179706 2201 36-37 0.45377100769869116 2201 38-39 0.4903987059917583 2201 40-41 0.5082386474480671 2201 42-43 0.5145019608906551 2201 44-45 0.4927938330270223 2201 46-47 0.44910974741117826 2201 48-49 0.4632351878102767 2201 50-51 0.40691568040323745 2201 52-53 0.5719345862049394 2201 54-55 0.555916758867518 2201 56-57 0.5651876197485777 2201 58-59 0.5829508422185938 2201 60-61 0.6516870987655636 2201 62-63 0.6504093344329434 2201 64-65 0.6211011923569458 2201 66-67 0.5350639870694991 2201 68-69 0.504810192876846 2201 70-71 0.5551323787485387 2201 72-73 0.5037035741723628 2201 74-75 0.4332273523574983 2201 76-77 0.4742038661747898 2201 78-79 0.41046498960135835 2201 80-81 0.4626442992517994 2201 82-83 0.48300645680566134 2201 84-85 0.5419077713182503 2201 86-87 0.5096846529068699 2201 88-89 0.48659573481240415 2201 90-91 0.46621896143722097 2201 92-93 0.5567486140191527 2201 94-95 0.4360852337599681 2201 96-97 0.4041738464913287 2201 98-99 0.40613032364326074 2201 100-101 0.3363648070842835 1101 1 0.16605746787276843 1101 2 0.18850391450905946 1101 3 0.09332700036057773 1101 4 0.13076236145705877 1101 5 0.25644839412436227 1101 6 0.18005602675459187 1101 7 0.26220730175879225 1101 8 0.22638740177198713 1101 9 0.20842745793977002 1101 10-11 0.22310665273047192 1101 12-13 0.1669753448001785 1101 14-15 0.34288427147421885 1101 16-17 0.32012204737628025 1101 18-19 0.4091164623012915 1101 20-21 0.3520326578685271 1101 22-23 0.38828034987117377 1101 24-25 0.3886428444874497 1101 26-27 0.4157131825114959 1101 28-29 0.35704056186894206 1101 30-31 0.3557940855263979 1101 32-33 0.29702353505022927 1101 34-35 0.18446547874978592 1101 36-37 0.30728386578864075 1101 38-39 0.22327435878262492 1101 40-41 0.3396455934917739 1101 42-43 0.1784525910979653 1101 44-45 0.20199047864154807 1101 46-47 0.1822044510334635 1101 48-49 0.21078724630654477 1101 50-51 0.2827744372025336 1101 52-53 0.3717470360461732 1101 54-55 0.3366338886425382 1101 56-57 0.3904641471919561 1101 58-59 0.3687596437973113 1101 60-61 0.3783511096832086 1101 62-63 0.4363164181103585 1101 64-65 0.3517784858966877 1101 66-67 0.42772277953724114 1101 68-69 0.32515730121202324 1101 70-71 0.4313798053858946 1101 72-73 0.33996336845842734 1101 74-75 0.3192976579818705 1101 76-77 0.38593326279637097 1101 78-79 0.529861553023359 1101 80-81 0.49975761637925586 1101 82-83 0.42243279563270875 1101 84-85 0.33902783443590323 1101 86-87 0.35313357917009824 1101 88-89 0.4346440500595037 1101 90-91 0.39910798989832585 1101 92-93 0.4167478967730638 1101 94-95 0.28302550073058796 1101 96-97 0.19382883173932086 1101 98-99 0.38389482267594843 1101 100-101 0.2749720314970112 2304 1 0.26596914242855263 2304 2 0.2131604704826806 2304 3 0.204137053338318 2304 4 0.20095681763344686 2304 5 0.31003810491110784 2304 6 0.32137954147637515 2304 7 0.3142487761628132 2304 8 0.2797859160118392 2304 9 0.21417360640712957 2304 10-11 0.19068013021186658 2304 12-13 0.20948573267856574 2304 14-15 0.4188939241834788 2304 16-17 0.3608021357718272 2304 18-19 0.3291997668645976 2304 20-21 0.34105327064381896 2304 22-23 0.23414552738955052 2304 24-25 0.41540733800937346 2304 26-27 0.46625334342503777 2304 28-29 0.3869456032539702 2304 30-31 0.342145876657753 2304 32-33 0.25327251641601833 2304 34-35 0.32006584642197566 2304 36-37 0.2955087171337638 2304 38-39 0.22308714914814232 2304 40-41 0.28419443273070755 2304 42-43 0.2843188726843806 2304 44-45 0.24302483268287034 2304 46-47 0.20328106634635645 2304 48-49 0.3003947238264928 2304 50-51 0.21509112247478868 2304 52-53 0.20810929829914215 2304 54-55 0.33377001497596126 2304 56-57 0.3963701664658359 2304 58-59 0.3877660000748122 2304 60-61 0.433987859026459 2304 62-63 0.5086657785395161 2304 64-65 0.44272473355492536 2304 66-67 0.3669907123187741 2304 68-69 0.39200397770431294 2304 70-71 0.43304006962618047 2304 72-73 0.4331867564752585 2304 74-75 0.3625609151228275 2304 76-77 0.4118849081206406 2304 78-79 0.3646839470284462 2304 80-81 0.40546630539483175 2304 82-83 0.33902137431157797 2304 84-85 0.44431811670930443 2304 86-87 0.36779073975241516 2304 88-89 0.2991418655819942 2304 90-91 0.2918370445882488 2304 92-93 0.4641276190736683 2304 94-95 0.3928749268780791 2304 96-97 0.377125815530551 2304 98-99 0.3652329181059848 2304 100-101 0.4603101391944726 2202 1 0.2543820507734793 2202 2 0.23950982893277128 2202 3 0.23014187091161986 2202 4 0.2713417395808406 2202 5 0.3404315293994671 2202 6 0.3458599806330156 2202 7 0.272544932542381 2202 8 0.26536627042302285 2202 9 0.230567033295074 2202 10-11 0.2861214324843999 2202 12-13 0.23618461115101752 2202 14-15 0.397023317179638 2202 16-17 0.4466343869575198 2202 18-19 0.35243268009668327 2202 20-21 0.39583648984339703 2202 22-23 0.40041353579929506 2202 24-25 0.42355108054314883 2202 26-27 0.4616471738437369 2202 28-29 0.3998790438755577 2202 30-31 0.40238477966199326 2202 32-33 0.3060428210317383 2202 34-35 0.3894230647233101 2202 36-37 0.3585186646267715 2202 38-39 0.34819428076435344 2202 40-41 0.333914760722422 2202 42-43 0.37788279711494965 2202 44-45 0.4141114742151899 2202 46-47 0.40318100351714037 2202 48-49 0.4896453884124483 2202 50-51 0.4142835610523292 2202 52-53 0.4657108138790633 2202 54-55 0.4592096895278104 2202 56-57 0.4802758482508409 2202 58-59 0.47325305049695743 2202 60-61 0.4806053794599592 2202 62-63 0.4781191403426277 2202 64-65 0.48331524822260974 2202 66-67 0.45285613781176437 2202 68-69 0.4340381846343959 2202 70-71 0.5472702477276172 2202 72-73 0.47033080255695126 2202 74-75 0.483187095670246 2202 76-77 0.5539926424334283 2202 78-79 0.4986368007187494 2202 80-81 0.49115196173620035 2202 82-83 0.47720290236136265 2202 84-85 0.5712365375082911 2202 86-87 0.47130277708680524 2202 88-89 0.5142653503920016 2202 90-91 0.44021670263892076 2202 92-93 0.5043949691963867 2202 94-95 0.5481736976090161 2202 96-97 0.43110851209982215 2202 98-99 0.4593434487389274 2202 100-101 0.4828336541593039 1102 1 0.05951838284639166 1102 2 0.004782864534970344 1102 3 0.014673782805200375 1102 4 -0.005308739673701268 1102 5 0.09590895698062951 1102 6 0.062126775768000186 1102 7 0.12732974558610977 1102 8 0.09314935050901596 1102 9 -0.03328017835622177 1102 10-11 0.0758290015525418 1102 12-13 0.04864589250875895 1102 14-15 0.0764060097380792 1102 16-17 0.05992642293269057 1102 18-19 0.09636325338275498 1102 20-21 -0.022597203676276933 1102 22-23 0.06926335522602045 1102 24-25 0.051653082465627165 1102 26-27 0.09316363694220087 1102 28-29 0.04602452059813089 1102 30-31 0.10554428151624506 1102 32-33 0.08279425682437136 1102 34-35 0.05175128509852556 1102 36-37 0.07527781003450684 1102 38-39 0.09653627762289574 1102 40-41 0.10227365126559818 1102 42-43 0.15469438609922292 1102 44-45 0.19393023003340915 1102 46-47 0.1341741490097803 1102 48-49 0.04187439202997467 1102 50-51 0.06262967046367862 1102 52-53 0.11526093471609755 1102 54-55 0.14682178975735383 1102 56-57 0.3233342192099258 1102 58-59 0.41423609844060394 1102 60-61 0.3128197446637415 1102 62-63 0.34257497190459674 1102 64-65 0.33642625227678735 1102 66-67 0.3046361719554298 1102 68-69 0.20020106674172666 1102 70-71 0.38300685331371653 1102 72-73 0.3324843626252374 1102 74-75 0.29224441518034894 1102 76-77 0.3381224527935771 1102 78-79 0.2608415687025456 1102 80-81 0.2865636794414783 1102 82-83 0.3404179369447107 1102 84-85 0.31911667763073837 1102 86-87 0.19770449427659287 1102 88-89 0.15990847026173327 1102 90-91 0.2886261870574671 1102 92-93 0.35058562714418784 1102 94-95 0.3464227356127978 1102 96-97 0.20643031461844785 1102 98-99 0.28607545887892627 1102 100-101 0.23797180561041031 2305 1 0.052610921442351355 2305 2 0.0780897752583094 2305 3 0.07361137769484927 2305 4 0.07232586919123918 2305 5 0.08663389478542882 2305 6 0.09705138677844616 2305 7 0.12612132711027613 2305 8 0.10801420117229554 2305 9 0.11095174572878364 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74-75 -0.24242791724810786 1213 76-77 -0.09318984624323434 1213 78-79 -0.06767275530928885 1213 80-81 -0.06310358236520841 1213 82-83 -0.1514520027762245 1213 84-85 -0.1890497170078902 1213 86-87 -0.09084414771772487 1213 88-89 -0.08248712601388064 1213 90-91 -0.18661833239004721 1213 92-93 -0.15543402740599177 1213 94-95 -0.05163955479011406 1213 96-97 0.013447019070451205 1213 98-99 -0.11805520919122614 1213 100-101 0.17104940141260272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 853.0 3 365.0 4 233.0 5 294.0 6 792.0 7 4482.0 8 15120.0 9 25090.0 10 25215.0 11 24655.0 12 18688.0 13 14151.0 14 12011.0 15 11115.0 16 11336.0 17 11904.0 18 13352.0 19 15435.0 20 17572.0 21 20284.0 22 23411.0 23 26027.0 24 30001.0 25 35180.0 26 42175.0 27 50912.0 28 62141.0 29 77808.0 30 99400.0 31 131986.0 32 183811.0 33 266359.0 34 406642.0 35 690066.0 36 1294450.0 37 1797513.0 38 314461.0 39 93.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.08830370510736 23.834497014271662 24.875719014609 15.20148026601198 2 32.07661221761945 21.611086396945282 33.45937557905753 12.852925806377735 3 40.59096588965602 18.80250927465824 24.698673344004305 15.907851491681427 4 31.453059075388794 17.533839421608118 34.29968422089553 16.713417282107564 5 33.934886744548834 16.374143130246978 32.84926689309547 16.841703232108717 6 26.53899641640447 17.079614978038794 39.365490854107314 17.015897751449415 7 34.43209352405615 17.1710346001632 29.75117540423834 18.645696471542312 8 29.948982952713454 25.789680792419727 26.018330080122592 18.243006174744224 9 31.981297016802767 24.8269864856456 24.25307404806818 18.93864244948345 10-11 26.824280512311578 27.703322909372545 28.01836400977124 17.45403256854464 12-13 27.351818691711756 25.08514438596581 27.763150173695156 19.799886748627284 14-15 26.401451936456976 25.69315621534457 27.796141256984175 20.109250591214277 16-17 25.057402311206754 27.01829334136657 28.936172720508157 18.988131626918516 18-19 24.092297098531116 27.2440902848676 29.068270350591806 19.595342266009478 20-21 23.780715082948717 28.059769839614308 28.674987956058917 19.484527121378054 22-23 24.338209012258012 27.474055758726895 28.472633390854817 19.71510183816027 24-25 24.32688066684411 27.092932549071286 28.954923244811493 19.62526353927311 26-27 23.17254236032605 27.22852357513059 29.31137748703166 20.2875565775117 28-29 22.53247162326444 27.825453017803554 28.400045788565492 21.242029570366512 30-31 21.720799630325004 28.709707298999632 28.502580370313325 21.066912700362042 32-33 21.559719744473615 27.55330095976387 29.514168287788788 21.372811007973727 34-35 21.79887925203885 27.779677732907604 29.303268235144692 21.118174779908852 36-37 21.268130131777106 29.137232432683803 29.51236762728508 20.082269808254015 38-39 21.859816023442804 28.486997789765244 28.039615041742856 21.613571145049097 40-41 22.243264923395177 27.77509309580578 27.639420740293197 22.34222124050584 42-43 23.233584228218174 28.066469978127433 27.969467779069618 20.730478014584776 44-45 21.725100170171086 28.888101340937748 28.031190369088637 21.355608119802525 46-47 22.065589474862488 28.640346881098466 28.65447341772811 20.639590226310936 48-49 22.3893457337072 28.124021206915966 28.6847519719888 20.801881087388036 50-51 21.308519227273536 28.538107697373533 27.690805294327674 22.46256778102526 52-53 21.768213663185275 28.62749987181312 27.92204259864598 21.682243866355616 54-55 22.312512279718653 28.569341755033655 27.960979011819326 21.157166953428366 56-57 22.762597002956305 28.334839622299413 27.093014399127767 21.80954897561651 58-59 21.96107017083187 27.697489028013837 27.872891601643236 22.46854919951106 60-61 20.949603150339737 27.671958429114536 28.66157828343604 22.71686013710968 62-63 21.40452496253103 27.773429800331144 28.569168930818712 22.25287630631911 64-65 21.77167894210892 28.47094059439857 28.22498657317043 21.532393890322076 66-67 22.089850914014555 28.020921250761415 28.009142790927744 21.88008504429629 68-69 21.576235132886552 26.941582854157954 29.493576067922717 21.988605945032777 70-71 21.277902463221647 28.761486188822712 28.926360044539877 21.034251303415765 72-73 22.35493042467434 29.40641994683462 27.87665777570565 20.361991852785387 74-75 23.3491501907735 28.561775927852935 27.55070239334027 20.5383714880333 76-77 23.38593941824335 27.24584180778809 27.966672020475368 21.401546753493186 78-79 23.24728786628136 27.341414303997986 28.69118940507805 20.720108424642603 80-81 22.257195726465778 28.70197139436595 28.144241666923957 20.896591212244314 82-83 21.433413305055222 28.680523843694957 28.556532396528343 21.329530454721475 84-85 21.622660954034945 27.92195647515933 28.95087211899716 21.50451045180856 86-87 20.798100626276984 28.49605384427467 29.45324471717598 21.252600812272362 88-89 20.7370884247224 29.13850070499493 29.286631193084627 20.837779677198036 90-91 21.523107117076375 28.106602700119687 28.618706328979094 21.75158385382485 92-93 21.42571695534055 28.562436485183042 27.756644957224903 22.255201602251507 94-95 21.14512909706422 29.71722793091292 27.230198778648067 21.907444193374797 96-97 20.99872155592022 30.580668607172466 26.519761241461136 21.90084859544617 98-99 21.347542277681637 29.11233133384513 27.37848446601521 22.161641922458024 100-101 22.040751384006978 29.07675204889944 27.105168914264617 21.77732765282897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 335.0 1 359.0 2 414.5 3 481.0 4 460.0 5 398.5 6 445.0 7 546.0 8 713.0 9 933.5 10 1138.0 11 1359.0 12 1567.5 13 1622.5 14 1474.5 15 1285.5 16 1140.0 17 1044.0 18 1024.5 19 1040.5 20 1091.5 21 1231.5 22 1465.5 23 1800.0 24 2198.5 25 2771.5 26 3599.5 27 4639.0 28 5844.0 29 7234.5 30 9094.5 31 12220.5 32 18757.5 33 30309.0 34 49408.0 35 82382.0 36 126644.0 37 189917.0 38 273986.5 39 329646.5 40 347638.0 41 337582.0 42 348212.0 43 392210.0 44 389120.0 45 371608.0 46 388688.0 47 374664.5 48 310041.5 49 251863.0 50 203568.5 51 168038.0 52 150530.0 53 138596.5 54 113240.5 55 72555.0 56 47548.0 57 38610.5 58 33740.5 59 28876.0 60 22901.5 61 17820.5 62 13122.0 63 9373.5 64 7072.5 65 5419.0 66 4501.5 67 3826.5 68 3004.0 69 2277.0 70 1716.0 71 1321.5 72 1003.5 73 767.5 74 592.0 75 418.0 76 309.5 77 233.5 78 164.0 79 132.5 80 117.0 81 91.5 82 74.0 83 61.5 84 54.5 85 44.5 86 34.0 87 30.0 88 24.0 89 20.0 90 15.5 91 9.0 92 9.5 93 10.5 94 8.0 95 4.0 96 4.0 97 4.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21877336966223712 2 0.20270517124145707 3 0.1768021272355444 4 0.16149578305023232 5 0.17178081522905062 6 0.13353226963475842 7 0.12019982051406806 8 0.11640786420571587 9 0.10451254921102203 10-11 0.08975162339882913 12-13 0.11773245168329095 14-15 0.11316132627048284 16-17 0.10416625183126384 18-19 0.09813201998897735 20-21 0.10188934655935374 22-23 0.0878816175481349 24-25 0.40506404510315597 26-27 0.19144184896482191 28-29 0.09321459719641104 30-31 0.1786721330862386 32-33 0.21024579668569168 34-35 0.20931945119483852 36-37 0.2146004862361509 38-39 0.2150593302643305 40-41 0.2118301071980854 42-43 0.22756066567360123 44-45 0.2089125517736226 46-47 0.22817534352267202 48-49 0.21723234632231317 50-51 0.22163898047973615 52-53 0.21318932441363628 54-55 0.1957532513428114 56-57 0.20379600798769537 58-59 0.19911233592646585 60-61 0.17817865932008317 62-63 0.1664218632772926 64-65 0.1709756738211128 66-67 0.18392719582406916 68-69 0.17632596833837688 70-71 0.1690450659289609 72-73 0.17275044789237354 74-75 0.16246541571355527 76-77 0.17344304265188992 78-79 0.17148646245625615 80-81 0.1770445354013751 82-83 0.20222035490979562 84-85 0.19995210707237943 86-87 0.20429813918834472 88-89 0.21160501390124256 90-91 0.19254134314555416 92-93 0.17307943040314383 94-95 0.17402309076298492 96-97 0.18289696111928852 98-99 0.19752802541407208 100-101 0.20855759695937048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5775383.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.03069640941936 #Duplication Level Percentage of deduplicated Percentage of total 1 84.70567471219933 27.97887425573687 2 6.454532945916787 4.263954364023452 3 2.3797076322014323 2.358102010272711 4 1.280431204339189 1.6917413753469988 5 0.759411505757358 1.254194544824566 6 0.6439306747354333 1.2761687175539203 7 0.4573180759918781 1.057387417143925 8 0.40048920895100776 1.0582749980887403 9 0.2680987631195252 0.7969939967107686 >10 2.0672132265015257 13.807366551116584 >50 0.27219903432154435 6.22103989591864 >100 0.2597706985854248 17.723095034512117 >500 0.032114236755299216 7.311624494780281 >1k 0.018419150418638897 10.700695900351858 >5k 4.2396353222957147E-4 0.9614938232695411 >10k+ 2.649666734553702E-4 1.5389926203490152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28059 0.485837908931754 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23645 0.4094100772191212 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 14183 0.2455767868555211 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11298 0.19562338982540206 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10737 0.1859097483231848 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8801 0.15238816196259192 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7559 0.13088309467960826 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7320 0.12674484099149788 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7244 0.12542891094841677 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6901 0.11948991088556378 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6517 0.11284100119420652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.233352835647437E-4 0.0 0.0 2 6.406501525526532E-4 0.0 0.0 3 6.925947595163819E-4 0.0 0.0 4 7.099096285042914E-4 0.0 0.0 5 8.137988424317487E-4 0.0 0.0 6 9.350029253471155E-4 0.0 0.0 7 0.0011427813532020302 0.0 0.0 8 0.001333244912069035 0.0 0.0 9 0.0015063936019481306 0.0 0.0 10-11 0.0025452857412227034 0.0 0.0 12-13 0.003480288666569819 0.0 0.0 14-15 0.00458844028179603 0.0 0.0 16-17 0.005670619593540377 0.0 0.0 18-19 0.007306874712897829 0.0 0.0 20-21 0.009202852867073924 0.0 0.0 22-23 0.011246007407647252 0.0 0.0 24-25 0.013877867493809502 0.0 0.0 26-27 0.016059540986286105 0.0 0.0 28-29 0.018726030810424177 0.0 0.0 30-31 0.02998069565256538 0.0 0.0 32-33 0.033867883740351074 0.0 0.0 34-35 0.03727891293096925 0.0 0.0 36-37 0.04126999023268241 0.0 0.0 38-39 0.04593634742492403 1.7314868987909548E-5 0.0 40-41 0.05175414340486163 1.7314868987909548E-5 0.0 42-43 0.057892264461075565 1.7314868987909548E-5 0.0 44-45 0.06429010855210815 1.7314868987909548E-5 0.0 46-47 0.07140651970613897 1.7314868987909548E-5 0.0 48-49 0.08046219618681566 1.7314868987909548E-5 0.0 50-51 0.09066065402069438 1.7314868987909548E-5 0.0 52-53 0.10153439174510158 1.7314868987909548E-5 0.0 54-55 0.11445994144457605 1.7314868987909548E-5 0.0 56-57 0.13029438913401933 1.7314868987909548E-5 0.0 58-59 0.14686471875544876 1.7314868987909548E-5 0.0 60-61 0.1652704244895966 1.7314868987909548E-5 0.0 62-63 0.18746808653209668 1.7314868987909548E-5 0.0 64-65 0.2121331174053738 1.7314868987909548E-5 0.0 66-67 0.23886727512270617 1.7314868987909548E-5 8.657434493954774E-6 68-69 0.26894320255470505 1.7314868987909548E-5 1.7314868987909548E-5 70-71 0.30197997258363646 1.7314868987909548E-5 1.7314868987909548E-5 72-73 0.3403064350883742 1.7314868987909548E-5 1.7314868987909548E-5 74-75 0.38287504049514987 1.7314868987909548E-5 4.328717246977386E-5 76-77 0.4292356022102777 1.7314868987909548E-5 6.925947595163819E-5 78-79 0.4826173433000028 1.7314868987909548E-5 6.925947595163819E-5 80-81 0.5418601675421353 1.7314868987909548E-5 6.925947595163819E-5 82-83 0.6099682047060775 1.7314868987909548E-5 6.925947595163819E-5 84-85 0.6848896428167621 1.7314868987909548E-5 6.925947595163819E-5 86-87 0.7700095387613255 1.7314868987909548E-5 6.925947595163819E-5 88-89 0.8611463516791873 1.7314868987909548E-5 6.925947595163819E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 875 0.0 60.73635 9 CGGGAGA 1220 0.0 55.652195 4 TTGTGTT 72265 0.0 49.907894 1 AGAGCGG 1270 0.0 42.967155 8 TCGGGAA 465 0.0 42.885822 3 CGGAAAG 525 0.0 38.88797 5 GATCCGG 525 0.0 37.08952 1 GTACCCC 210 0.0 36.1849 1 AACGGTG 95 8.718489E-7 34.994606 1 ATTCGGA 235 0.0 34.35223 2 TCGGGAG 2140 0.0 33.724632 3 TCGGAAA 465 0.0 33.695126 4 TGTGTTT 108250 0.0 33.361473 2 CGTGGCA 205 0.0 32.434025 1 GAACCGG 280 0.0 32.227173 1 GGATCGG 1095 0.0 29.926893 1 GTACGGG 495 0.0 29.74289 1 GGACCCC 800 0.0 29.682924 1 GTGACGC 195 1.6370905E-11 29.226261 1 CTACGGG 345 0.0 28.908585 1 >>END_MODULE