FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5775383
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA280590.485837908931754No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC236450.4094100772191212No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT141830.2455767868555211No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC112980.19562338982540206No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT107370.1859097483231848No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC88010.15238816196259192No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75590.13088309467960826No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT73200.12674484099149788No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG72440.12542891094841677No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT69010.11948991088556378No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA65170.11284100119420652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC8750.060.736359
CGGGAGA12200.055.6521954
TTGTGTT722650.049.9078941
AGAGCGG12700.042.9671558
TCGGGAA4650.042.8858223
CGGAAAG5250.038.887975
GATCCGG5250.037.089521
GTACCCC2100.036.18491
AACGGTG958.718489E-734.9946061
ATTCGGA2350.034.352232
TCGGGAG21400.033.7246323
TCGGAAA4650.033.6951264
TGTGTTT1082500.033.3614732
CGTGGCA2050.032.4340251
GAACCGG2800.032.2271731
GGATCGG10950.029.9268931
GTACGGG4950.029.742891
GGACCCC8000.029.6829241
GTGACGC1951.6370905E-1129.2262611
CTACGGG3450.028.9085851
Produced by FastQC (version 0.11.2)