##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8355919 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07115830107975 33.0 33.0 33.0 27.0 33.0 2 30.72010343805391 33.0 33.0 33.0 27.0 33.0 3 30.55148428317699 33.0 33.0 33.0 27.0 33.0 4 34.019579414305 37.0 37.0 37.0 27.0 37.0 5 34.049721161729785 37.0 37.0 37.0 27.0 37.0 6 34.493686930186854 37.0 37.0 37.0 27.0 37.0 7 33.985306583273484 37.0 37.0 37.0 27.0 37.0 8 34.24075209441355 37.0 37.0 37.0 27.0 37.0 9 33.782998853866346 37.0 37.0 37.0 22.0 37.0 10-11 33.802063662895726 37.0 37.0 37.0 22.0 37.0 12-13 33.78291065291562 37.0 37.0 37.0 24.5 37.0 14-15 35.318972156144646 40.0 37.0 40.0 22.0 40.0 16-17 35.22830062139185 40.0 37.0 40.0 22.0 40.0 18-19 35.25341407689567 40.0 37.0 40.0 22.0 40.0 20-21 35.251419442912265 40.0 37.0 40.0 22.0 40.0 22-23 35.82820202062753 40.0 37.0 40.0 27.0 40.0 24-25 35.278326836342 40.0 37.0 40.0 22.0 40.0 26-27 35.114007747083235 37.0 37.0 40.0 21.0 40.0 28-29 34.8931629184055 37.0 37.0 40.0 18.5 40.0 30-31 34.893983115441884 37.0 37.0 40.0 18.5 40.0 32-33 34.71694370182382 37.0 37.0 40.0 15.0 40.0 34-35 34.782299828421024 37.0 37.0 40.0 18.5 40.0 36-37 34.63959499846755 37.0 37.0 40.0 15.0 40.0 38-39 34.7673020166902 37.0 37.0 40.0 18.5 40.0 40-41 34.4174773594622 37.0 37.0 40.0 15.0 40.0 42-43 34.29382261843371 37.0 37.0 40.0 15.0 40.0 44-45 34.1769805930383 37.0 33.0 40.0 15.0 40.0 46-47 34.10772728888348 37.0 33.0 40.0 15.0 40.0 48-49 34.00483854618504 37.0 33.0 40.0 15.0 40.0 50-51 32.99100649491696 37.0 33.0 38.5 15.0 38.5 52-53 33.439051108561486 37.0 33.0 38.5 18.5 40.0 54-55 34.12893153942732 37.0 33.0 40.0 18.5 40.0 56-57 34.33527407338438 37.0 33.0 40.0 18.5 40.0 58-59 33.88021209875299 37.0 33.0 40.0 15.0 40.0 60-61 33.71505533981361 37.0 33.0 40.0 15.0 40.0 62-63 33.400672744673564 37.0 33.0 40.0 15.0 40.0 64-65 33.18640600752592 37.0 33.0 38.5 15.0 40.0 66-67 33.01701566278946 37.0 33.0 37.0 15.0 40.0 68-69 33.03137751814013 37.0 33.0 37.0 18.5 40.0 70-71 32.639773973395386 37.0 33.0 37.0 15.0 40.0 72-73 32.79435732921777 37.0 33.0 37.0 22.0 37.0 74-75 32.74490519833904 37.0 33.0 37.0 22.0 37.0 76-77 32.430066100449274 37.0 33.0 37.0 22.0 37.0 78-79 32.00684508789518 37.0 33.0 37.0 22.0 37.0 80-81 31.571703962185367 35.0 33.0 37.0 15.0 37.0 82-83 31.285802973915857 33.0 33.0 37.0 15.0 37.0 84-85 31.082995239661848 33.0 33.0 37.0 15.0 37.0 86-87 30.927475003048738 33.0 33.0 37.0 15.0 37.0 88-89 30.75949904492851 33.0 33.0 37.0 15.0 37.0 90-91 30.622136655465425 33.0 30.0 37.0 15.0 37.0 92-93 30.416204010594164 33.0 27.0 37.0 15.0 37.0 94-95 30.276566347758994 33.0 27.0 37.0 15.0 37.0 96-97 30.145437802831744 33.0 27.0 37.0 15.0 37.0 98-99 29.994816907631584 33.0 27.0 37.0 15.0 37.0 100-101 28.756563939885005 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07141139443057298 2306 2 0.07566348523746314 2306 3 0.09095990884588545 2306 4 0.23060047124128147 2306 5 0.25893620458650446 2306 6 0.01977711021690709 2306 7 0.37013740153588515 2306 8 -0.020130334768325042 2306 9 0.23422311511474447 2306 10-11 0.1594502264080404 2306 12-13 0.13279118368367193 2306 14-15 0.47757533836873023 2306 16-17 0.4350582025966361 2306 18-19 0.48589549808765753 2306 20-21 0.4262345439492563 2306 22-23 0.24155965134707458 2306 24-25 0.41437715193981006 2306 26-27 0.17859179962427163 2306 28-29 0.3168739324212311 2306 30-31 0.35474337146527546 2306 32-33 0.4529822361050151 2306 34-35 0.24673393958411793 2306 36-37 0.3858523043935307 2306 38-39 0.1831394769388055 2306 40-41 0.30589798382820277 2306 42-43 0.24944590950859435 2306 44-45 0.41887941059746225 2306 46-47 0.33784083579919155 2306 48-49 0.38717604635752423 2306 50-51 0.36507220452528344 2306 52-53 0.35544019645066527 2306 54-55 0.3644697399019421 2306 56-57 0.2702730690181454 2306 58-59 0.5279117590262103 2306 60-61 0.47935233706186153 2306 62-63 0.5183482156479187 2306 64-65 0.4633917539401793 2306 66-67 0.4382166291557681 2306 68-69 0.36141071665840485 2306 70-71 0.4583372431937107 2306 72-73 0.15125909062282972 2306 74-75 0.1089141626351946 2306 76-77 0.2397950133495641 2306 78-79 0.16272764748930513 2306 80-81 0.42902439435559003 2306 82-83 0.5002851974216256 2306 84-85 0.49908587322527964 2306 86-87 0.3905091273621686 2306 88-89 0.396714272723667 2306 90-91 0.4443544410902298 2306 92-93 0.5036983433671338 2306 94-95 0.3374679007104575 2306 96-97 0.36646397916324247 2306 98-99 0.35960068234809484 2306 100-101 0.42321942237442656 2307 1 0.10569439293093552 2307 2 0.10441929931125671 2307 3 0.11870621822076544 2307 4 0.23541517437207204 2307 5 0.25495754048856156 2307 6 -0.010870831654571589 2307 7 0.3410278334636416 2307 8 0.0014466098377710068 2307 9 0.20201403223154557 2307 10-11 0.17613638140871757 2307 12-13 0.18417355834241533 2307 14-15 0.46359169647343634 2307 16-17 0.43898887061165226 2307 18-19 0.4237909534471527 2307 20-21 0.3712909998885081 2307 22-23 0.2254109999932652 2307 24-25 0.366067351307116 2307 26-27 0.13747311427059117 2307 28-29 0.2977881107265432 2307 30-31 0.42651635901488305 2307 32-33 0.42894077198781844 2307 34-35 0.26567448597023 2307 36-37 0.21821435745328444 2307 38-39 0.14353356164212983 2307 40-41 0.3783453657012359 2307 42-43 0.26898818757238985 2307 44-45 0.35609583571924475 2307 46-47 0.44559006767442355 2307 48-49 0.3872667778228305 2307 50-51 0.21347272162839914 2307 52-53 0.2684212237902628 2307 54-55 0.3487613879437319 2307 56-57 0.28206387347348283 2307 58-59 0.37011238430233817 2307 60-61 0.3724861281486156 2307 62-63 0.446375246502015 2307 64-65 0.4358168109494329 2307 66-67 0.3970718402308293 2307 68-69 0.27888298276977963 2307 70-71 0.3008669434097513 2307 72-73 0.10043587470383386 2307 74-75 0.07218902446870601 2307 76-77 0.2459518324096095 2307 78-79 0.2663683346922454 2307 80-81 0.42772021544998395 2307 82-83 0.3778437285260594 2307 84-85 0.5045907027983283 2307 86-87 0.3528118342212885 2307 88-89 0.408609828848693 2307 90-91 0.41804870146846795 2307 92-93 0.36630441396068747 2307 94-95 0.3429771787618989 2307 96-97 0.30505424686217353 2307 98-99 0.2883291732677762 2307 100-101 0.08024111310511017 2201 1 0.013547103690804363 2201 2 -0.06958371726134871 2201 3 -0.02427867255525129 2201 4 0.06459338097644007 2201 5 0.06369650816296968 2201 6 -0.05598169301561029 2201 7 0.28127810139247345 2201 8 -0.02327707897551079 2201 9 0.18162440151376558 2201 10-11 0.0763651597810977 2201 12-13 0.08423856406340491 2201 14-15 0.2760456379758338 2201 16-17 0.19599362508704843 2201 18-19 0.18487385881307716 2201 20-21 0.28002374722219514 2201 22-23 0.1401001625209588 2201 24-25 0.3525228813023844 2201 26-27 0.13139295947986795 2201 28-29 0.25514684402637755 2201 30-31 0.2759337914483311 2201 32-33 0.2765787234360104 2201 34-35 0.11793186564245417 2201 36-37 0.17962230547080793 2201 38-39 0.09011691835149094 2201 40-41 0.261373310037591 2201 42-43 0.22575988645957068 2201 44-45 0.3283483753717533 2201 46-47 0.29807780128896155 2201 48-49 0.3044136538562867 2201 50-51 0.1991361824199558 2201 52-53 0.22741159670682265 2201 54-55 0.2860885288424342 2201 56-57 0.34907074384799586 2201 58-59 0.4767913212173198 2201 60-61 0.37148125165433754 2201 62-63 0.41082891400038335 2201 64-65 0.4112921870857278 2201 66-67 0.34250959606487186 2201 68-69 0.27607687633831546 2201 70-71 0.41743944109450837 2201 72-73 0.16284044210547677 2201 74-75 0.3230690961592444 2201 76-77 0.44015742466681473 2201 78-79 0.42448699603746576 2201 80-81 0.4580241960325928 2201 82-83 0.4823636078875033 2201 84-85 0.38549572468048865 2201 86-87 0.3874092779773015 2201 88-89 0.29630541665825305 2201 90-91 0.37350522086546434 2201 92-93 0.34845978519563303 2201 94-95 0.21544800859468083 2201 96-97 0.20371978127231927 2201 98-99 0.12887467209860048 2201 100-101 0.06710747354465951 1101 1 0.016547523524696572 1101 2 0.012139086988451453 1101 3 -0.011561602025640383 1101 4 0.08159423463432347 1101 5 0.13503767412444034 1101 6 0.028580840987210365 1101 7 0.2969723838720384 1101 8 0.06954746170777781 1101 9 0.1653227262183421 1101 10-11 0.12976081314709376 1101 12-13 0.18682535403126366 1101 14-15 0.36875113202068377 1101 16-17 0.30083314878021383 1101 18-19 0.3656761860866027 1101 20-21 0.28794054480093934 1101 22-23 0.24608200441107186 1101 24-25 0.3341479901669757 1101 26-27 0.2390075250354684 1101 28-29 0.33757052637098894 1101 30-31 0.47441255061234244 1101 32-33 0.3023299626389502 1101 34-35 0.15387237919435393 1101 36-37 0.22854295581977624 1101 38-39 0.15188264215047553 1101 40-41 0.4093419076132747 1101 42-43 0.22292525590358991 1101 44-45 0.26490236808879075 1101 46-47 0.22711642744499017 1101 48-49 0.25408971102116595 1101 50-51 0.3102822034184527 1101 52-53 0.29918096447045883 1101 54-55 0.3986893572919783 1101 56-57 0.44339578863267803 1101 58-59 0.5720259516163964 1101 60-61 0.5073206572814755 1101 62-63 0.5313919751817267 1101 64-65 0.49330989489730115 1101 66-67 0.5193048370235047 1101 68-69 0.4273859382782703 1101 70-71 0.5329673393898489 1101 72-73 0.3608138577686191 1101 74-75 0.4270840535423801 1101 76-77 0.5420569017114687 1101 78-79 0.6119839806469116 1101 80-81 0.5881746955643159 1101 82-83 0.5063564086057823 1101 84-85 0.42174300723339897 1101 86-87 0.4228448729614165 1101 88-89 0.5034609825690985 1101 90-91 0.5747496406768668 1101 92-93 0.5755242833699192 1101 94-95 0.5460070191830972 1101 96-97 0.4144987842956205 1101 98-99 0.5587349227202125 1101 100-101 0.4706449499625087 2304 1 0.13787130779143197 2304 2 0.056562569454044365 2304 3 0.06255340157357381 2304 4 0.08449941968329 2304 5 0.10519208726017126 2304 6 0.16291971948336226 2304 7 0.3453709266641596 2304 8 0.20140538249556528 2304 9 0.20963143663461636 2304 10-11 0.19502893164608537 2304 12-13 0.22921560411158026 2304 14-15 0.3729847102705932 2304 16-17 0.3077967854166346 2304 18-19 0.3693148130711137 2304 20-21 0.3593305346082545 2304 22-23 0.27843387224694993 2304 24-25 0.37394218956112724 2304 26-27 0.32268768177051754 2304 28-29 0.33375131693978943 2304 30-31 0.3645956471325462 2304 32-33 0.26485067054942135 2304 34-35 0.2714025480765514 2304 36-37 0.34256952837737487 2304 38-39 0.33718020022168105 2304 40-41 0.4657647857705314 2304 42-43 0.2691733572104482 2304 44-45 0.37182514852332105 2304 46-47 0.3950861640493528 2304 48-49 0.3812482258636294 2304 50-51 0.2904558834957598 2304 52-53 0.23597196324483605 2304 54-55 0.36434654942768674 2304 56-57 0.49238395728315965 2304 58-59 0.49875212163340166 2304 60-61 0.43086489157659713 2304 62-63 0.5043992720524528 2304 64-65 0.4067711980627067 2304 66-67 0.3837579536242046 2304 68-69 0.3108452922042986 2304 70-71 0.4075719655258112 2304 72-73 0.3975084295681768 2304 74-75 0.5508585078917463 2304 76-77 0.5300819066136526 2304 78-79 0.5871733104006154 2304 80-81 0.5529632546403427 2304 82-83 0.4316275891636785 2304 84-85 0.5422350351291492 2304 86-87 0.4406577176586701 2304 88-89 0.4676145445222595 2304 90-91 0.46232276623788593 2304 92-93 0.5490710810813155 2304 94-95 0.5539618735313105 2304 96-97 0.4394673312983244 2304 98-99 0.5159697078216858 2304 100-101 0.4915329614864099 2202 1 0.13518539366334892 2202 2 0.13441286557672072 2202 3 0.16213728527588245 2202 4 0.2068721310198498 2202 5 0.3165639846511823 2202 6 0.24927874563712749 2202 7 0.364820263277295 2202 8 0.18569379458842405 2202 9 0.3080921538382668 2202 10-11 0.256863920773462 2202 12-13 0.21435325238163472 2202 14-15 0.3530877090967621 2202 16-17 0.342508470800972 2202 18-19 0.37793804767930794 2202 20-21 0.37657208785423535 2202 22-23 0.31152325077747633 2202 24-25 0.3068743742161715 2202 26-27 0.3350560253085675 2202 28-29 0.3358148161176473 2202 30-31 0.3885197709876067 2202 32-33 0.40394972861653144 2202 34-35 0.27843088442573816 2202 36-37 0.2887357159337469 2202 38-39 0.2610024051162583 2202 40-41 0.3620253599588068 2202 42-43 0.3174740206705451 2202 44-45 0.3980298890054925 2202 46-47 0.349955450135468 2202 48-49 0.33476790871340967 2202 50-51 0.3560707045991123 2202 52-53 0.3812807341562916 2202 54-55 0.4276555790089063 2202 56-57 0.5272394508224281 2202 58-59 0.523760111687352 2202 60-61 0.4131962988748299 2202 62-63 0.5023291175478022 2202 64-65 0.480565800286918 2202 66-67 0.4815818924556865 2202 68-69 0.4008686296066273 2202 70-71 0.6152604095935246 2202 72-73 0.4274015118805323 2202 74-75 0.4489522538546069 2202 76-77 0.5230305832966664 2202 78-79 0.5193806411689224 2202 80-81 0.5229747091260428 2202 82-83 0.5276663349932491 2202 84-85 0.5439935518968362 2202 86-87 0.48129055666325726 2202 88-89 0.435510099430406 2202 90-91 0.43972875567314773 2202 92-93 0.49974761198590656 2202 94-95 0.5082785386307265 2202 96-97 0.4948861276695631 2202 98-99 0.5767727093429471 2202 100-101 0.521494144178444 1102 1 0.16927465195332658 1102 2 0.039293859823906985 1102 3 0.10579338943889027 1102 4 0.13046584229374503 1102 5 0.1551145812118122 1102 6 0.15628568205038107 1102 7 0.15718006586671152 1102 8 0.16733228417486146 1102 9 0.13796578800432968 1102 10-11 0.19654793851170638 1102 12-13 0.16373808495332298 1102 14-15 0.04677072744367905 1102 16-17 0.11305417075469393 1102 18-19 0.16016552991081312 1102 20-21 0.08464931454714275 1102 22-23 0.1967453039292124 1102 24-25 0.16824452782326915 1102 26-27 0.19287289772172755 1102 28-29 0.14660727686147368 1102 30-31 0.18469940435831234 1102 32-33 0.18793584486430603 1102 34-35 0.23830039221420662 1102 36-37 0.1709352383818299 1102 38-39 0.2217933627020443 1102 40-41 0.21516046049798376 1102 42-43 0.30991640648437624 1102 44-45 0.27951017468763695 1102 46-47 0.22360417803752597 1102 48-49 0.1611934044589347 1102 50-51 0.18699560122133363 1102 52-53 0.3309126570298062 1102 54-55 0.40639940201232605 1102 56-57 0.49999244093258 1102 58-59 0.44356836996108484 1102 60-61 0.40593026951237476 1102 62-63 0.393995508461515 1102 64-65 0.377308698639375 1102 66-67 0.45039185893630673 1102 68-69 0.5182399590173432 1102 70-71 0.41554015008861 1102 72-73 0.4503131112605132 1102 74-75 0.5394772067478257 1102 76-77 0.46273900125657264 1102 78-79 0.406241597623513 1102 80-81 0.3446482856223305 1102 82-83 0.43149207402072776 1102 84-85 0.4306167084013701 1102 86-87 0.37780725060772014 1102 88-89 0.3344206595436283 1102 90-91 0.2815992350862686 1102 92-93 0.3616684016115279 1102 94-95 0.4587360066869657 1102 96-97 0.2700510114063768 1102 98-99 0.3066542698840422 1102 100-101 0.35227321359348096 2305 1 0.18996197145452243 2305 2 0.1235252909657163 2305 3 0.13832573852090135 2305 4 0.1945495179214376 2305 5 0.18562053238446907 2305 6 0.29222926642441394 2305 7 0.23415456039523974 2305 8 0.31166651045554516 2305 9 0.14105738297935488 2305 10-11 0.24975823756388849 2305 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-0.29305669799759215 1213 70-71 -0.37695627779408625 1213 72-73 -0.28944342732783923 1213 74-75 -0.3056825958689373 1213 76-77 -0.2805541181710325 1213 78-79 -0.20967038492824486 1213 80-81 -0.4498759550707554 1213 82-83 -0.34950283735178544 1213 84-85 -0.41697913952351584 1213 86-87 -0.4179941904142872 1213 88-89 -0.43370944228422204 1213 90-91 -0.4440622995378867 1213 92-93 -0.3552645559281764 1213 94-95 -0.3399109730052885 1213 96-97 -0.33855440577139717 1213 98-99 -0.4015722752400137 1213 100-101 -0.11828065891702622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1183.0 3 682.0 4 667.0 5 570.0 6 1660.0 7 10960.0 8 56634.0 9 149259.0 10 171467.0 11 123022.0 12 76363.0 13 50367.0 14 35912.0 15 28828.0 16 25177.0 17 24876.0 18 25129.0 19 27169.0 20 29453.0 21 32554.0 22 35874.0 23 38786.0 24 43889.0 25 50114.0 26 59253.0 27 71690.0 28 87630.0 29 108021.0 30 132159.0 31 173404.0 32 237298.0 33 339465.0 34 515113.0 35 872744.0 36 1669017.0 37 2475899.0 38 573425.0 39 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.92170284991667 23.646882055110094 25.58121154320354 13.850203551769697 2 29.467767034741794 25.18220983939728 33.45895366541598 11.891069460444944 3 36.640187964061155 20.163780400564956 28.251279886548275 14.944751748825613 4 26.656794357008895 18.214399331809094 36.81200394221127 18.31680236897074 5 33.89573049052617 17.13717513181502 32.97411028609092 15.992984091567891 6 27.514584609308145 17.543820335396582 40.12828392627041 14.813311129024855 7 33.931913072380496 19.15124866133124 31.038411555841627 15.878426710446636 8 26.665496493073608 30.396113745665765 27.390365118123633 15.548024643136992 9 33.01572222315398 26.414652671124735 23.860653950561968 16.70897115515932 10-11 26.61620222064005 29.288823110601754 27.30097103892109 16.794003629837107 12-13 28.485583957795956 24.11300467589897 27.095226938325258 20.306184427979815 14-15 25.578372128896216 24.735577650281325 28.608670257933213 21.077379962889246 16-17 26.01204989231648 26.201061351304645 29.748229309145298 18.038659447233577 18-19 25.49952949416625 26.60126363054891 29.900536426363395 17.99867044892144 20-21 24.6292383223803 28.491498108501194 28.80897808750837 18.07028548161013 22-23 27.177896044269147 26.623495664184173 28.150968427925466 18.04763986362122 24-25 24.13493899178747 29.285323785092487 28.381026475942157 18.19871074717788 26-27 22.465881490769068 30.792056123651985 28.346919027019453 18.395143358559494 28-29 23.136416317010926 29.67985036050022 28.034000330964375 19.149732991524473 30-31 25.08277690175878 27.567402678472202 28.25156865210947 19.09825176765954 32-33 22.821406386035434 26.96604555560619 29.77974701456561 20.432801043792768 34-35 20.393003006703246 27.18078923786713 33.34829339835415 19.077914357075464 36-37 21.363416923446575 27.85799232979265 29.98901523457862 20.78957551218215 38-39 20.525867824505568 27.47333297114889 27.775229294533066 24.225569909812474 40-41 22.067185058421007 26.959559207110058 28.749833605361086 22.22342212910785 42-43 23.814185307453716 27.125954693581388 30.264388493846717 18.79547150511818 44-45 21.788199232981324 29.053530612391743 30.601245026250844 18.557025128376093 46-47 23.280916957037476 29.51982770682357 28.74258690088962 18.456668435249334 48-49 21.10211707296948 28.873015199116924 30.147847547335633 19.87702018057796 50-51 20.173608845665246 27.915862699908274 30.556119918098535 21.35440853632794 52-53 24.114134766104115 27.509152244307856 28.10147497846892 20.27523801111911 54-55 25.406643173433064 27.40268273702966 27.905543052209897 19.285131037327382 56-57 25.741740918552352 27.058648032601578 27.292934863452494 19.906676185393575 58-59 24.237580517256543 26.743567057979377 27.913650164192582 21.105202260571495 60-61 21.169900622614694 27.01998517041732 27.852539760844508 23.957574446123477 62-63 23.28596168893303 27.214037262204045 28.190270927287724 21.309730121575203 64-65 21.28537431919787 28.469348709985304 29.93680890357442 20.308468067242405 66-67 20.518038958275262 28.456978950302574 28.57292462811602 22.45205746330615 68-69 20.205339289053846 27.177722152690865 32.46449704851371 20.152441509741585 70-71 19.788922950115957 31.188347104535307 29.85229737069517 19.170432574653564 72-73 19.734155129020444 34.21220238327143 27.108264968571287 18.945377519136837 74-75 20.54449596183205 33.687832599648566 26.70503271330473 19.062638725214654 76-77 20.73397088732154 32.75514082372458 27.24009163697244 19.27079665198144 78-79 20.66661280254961 32.51621707510892 27.734959534182472 19.082210588158997 80-81 19.904761921887946 32.97266749996838 27.5081155806621 19.614454997481573 82-83 19.21707502279082 32.467149293432136 28.019628433949194 20.29614724982785 84-85 19.389194815553772 31.60849410196426 28.330482576589265 20.6718285058927 86-87 19.330928081927723 31.47289091720805 28.97682202470653 20.219358976157697 88-89 19.600333459193013 31.690369839775652 28.578691650562444 20.13060505046889 90-91 20.153978985469102 30.867353882009713 28.197918786162823 20.780748346358354 92-93 19.7881239788323 31.591691276986865 27.861054240963902 20.759130503216937 94-95 19.486357244668113 32.262188331281415 27.701021003885256 20.550433420165216 96-97 20.01519594678841 32.45645626924221 27.214999568399143 20.31334821557024 98-99 20.43067508393749 31.329497207199612 27.090673354886913 21.149154353975984 100-101 20.485000849035696 31.413481343759685 26.71361906022781 21.38789874697681 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 340.0 1 463.5 2 608.5 3 733.5 4 774.5 5 737.0 6 834.5 7 1039.0 8 1333.0 9 1688.5 10 2022.0 11 2283.5 12 2444.5 13 2444.0 14 2318.0 15 2237.0 16 2202.5 17 2214.5 18 2411.0 19 2753.0 20 3311.0 21 4066.0 22 5213.5 23 6966.5 24 9170.5 25 12123.5 26 16104.5 27 21193.0 28 27027.0 29 33290.5 30 40438.0 31 48787.0 32 60629.0 33 84795.0 34 125534.5 35 201894.0 36 303986.0 37 408437.0 38 527791.5 39 591280.5 40 586408.5 41 534874.0 42 479353.5 43 483436.5 44 474365.5 45 448172.0 46 451013.0 47 411155.5 48 326661.0 49 276143.5 50 237398.0 51 202196.0 52 180814.5 53 157565.0 54 125619.0 55 92872.5 56 71279.0 57 56583.0 58 45446.5 59 35894.0 60 27692.5 61 21421.0 62 16287.5 63 12264.5 64 9395.5 65 7048.0 66 5199.0 67 3908.0 68 2946.5 69 2137.5 70 1551.5 71 1163.0 72 865.5 73 636.5 74 492.5 75 370.5 76 286.0 77 252.0 78 196.5 79 141.0 80 117.5 81 102.0 82 88.0 83 70.5 84 54.5 85 46.5 86 44.0 87 29.0 88 18.5 89 18.5 90 17.5 91 13.0 92 10.0 93 9.0 94 8.0 95 6.0 96 2.0 97 0.5 98 2.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2153084538038246 2 0.20069605748930788 3 0.17652157710001737 4 0.16028159200681574 5 0.17119601087564396 6 0.1315474695242977 7 0.11896955918313712 8 0.11463730081634348 9 0.10171233110325748 10-11 0.08778208596804253 12-13 0.11500231153509267 14-15 0.11107695036297026 16-17 0.10222095259659651 18-19 0.09560288940091449 20-21 0.0994025911452708 22-23 0.08687853484458143 24-25 0.3981489049857951 26-27 0.18950040085357459 28-29 0.090815863581253 30-31 0.17379297238281033 32-33 0.20463338622597943 34-35 0.20406492690989464 36-37 0.21006067674902065 38-39 0.2109402927433835 40-41 0.20714657478130175 42-43 0.2228779383811643 44-45 0.2051120888079456 46-47 0.22420633804612036 48-49 0.21305855166858367 50-51 0.21655308051693659 52-53 0.20812193129205775 54-55 0.1906313357034696 56-57 0.1982845932326534 58-59 0.19350953497754106 60-61 0.17333820492994248 62-63 0.16217845098785663 64-65 0.16660046608876894 66-67 0.1788432846225532 68-69 0.17184225936129827 70-71 0.16457794767996195 72-73 0.16783312523733177 74-75 0.15812144660569352 76-77 0.16861700071530134 78-79 0.16682784981520285 80-81 0.17236284841918645 82-83 0.19730325293962278 84-85 0.19531663722446327 86-87 0.1994514307761959 88-89 0.20664992085251185 90-91 0.18777108777622187 92-93 0.1682579737788267 94-95 0.1690897195149929 96-97 0.1780653929268582 98-99 0.19246237307949013 100-101 0.20401107286942347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8355919.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.89495344194492 #Duplication Level Percentage of deduplicated Percentage of total 1 91.50271719865299 40.16507511246326 2 3.7643733903632097 3.3047398941617887 3 1.3645125632522686 1.7968564630472177 4 0.6664913570746754 1.1702242835300627 5 0.43649977550007485 0.9580068661497597 6 0.3720469427091139 0.9798589937060703 7 0.2670566789396098 0.8205708341890223 8 0.1852252350109448 0.6504362453662983 9 0.14043347433459608 0.5547888735846896 >10 0.9921022316435607 8.885517124649308 >50 0.13373295898622034 4.047063054506003 >100 0.13167941830780464 12.591348712778712 >500 0.023924946522294967 7.168212809053917 >1k 0.018590955876911192 14.651910283325147 >5k 4.180540147340934E-4 1.124458934646993 >10k+ 1.9481881091745827E-4 1.1309315148415484 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 17159 0.20535144009892867 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 16447 0.1968305341399312 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13131 0.1571460900949375 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13054 0.15622458762465266 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11643 0.13933835404579675 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10977 0.1313679560560604 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10163 0.12162635851304925 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9539 0.11415859823437732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0022379345706917456 0.0 0.0 2 0.0023456426516341292 0.0 0.0 3 0.002525156119871435 0.0 0.0 4 0.0026687668944612793 0.0 0.0 5 0.003075664089132506 0.0 0.0 6 0.003542399106549501 0.0 1.196756454915372E-5 7 0.004188647592203802 0.0 3.590269364746116E-5 8 0.004679317738719105 0.0 3.590269364746116E-5 9 0.005265728401627636 0.0 3.590269364746116E-5 10-11 0.008161879022522836 0.0 3.590269364746116E-5 12-13 0.0109383539979265 0.0 5.3854040471191736E-5 14-15 0.014014018087059005 0.0 5.98378227457686E-5 16-17 0.01633572560959483 0.0 7.778916956949919E-5 18-19 0.019560984255591753 0.0 8.377295184407603E-5 20-21 0.02317518874943618 0.0 1.0172429866780661E-4 22-23 0.02732194986571794 0.0 1.0770808094238347E-4 24-25 0.032270537806793 0.0 1.0770808094238347E-4 26-27 0.03750634729704776 1.196756454915372E-5 1.0770808094238347E-4 28-29 0.0436756268221365 1.196756454915372E-5 1.0770808094238347E-4 30-31 0.06852029082617962 1.196756454915372E-5 1.0770808094238347E-4 32-33 0.07585042411253627 1.196756454915372E-5 1.0770808094238347E-4 34-35 0.08225905492860809 1.196756454915372E-5 1.0770808094238347E-4 36-37 0.08855997766373752 1.196756454915372E-5 1.0770808094238347E-4 38-39 0.09545329484405007 1.196756454915372E-5 1.0770808094238347E-4 40-41 0.10324417936554914 1.196756454915372E-5 1.0770808094238347E-4 42-43 0.11158557185630928 2.393512909830744E-5 1.0770808094238347E-4 44-45 0.12036976423538812 2.393512909830744E-5 1.1369186321696033E-4 46-47 0.12978823753557212 2.393512909830744E-5 1.196756454915372E-4 48-49 0.1397931214986646 2.393512909830744E-5 1.3164321004069092E-4 50-51 0.1507793457547877 2.393512909830744E-5 1.495945568644215E-4 52-53 0.1618792618741278 2.393512909830744E-5 1.5557833913899834E-4 54-55 0.1741101128433629 2.393512909830744E-5 1.5557833913899834E-4 56-57 0.1870889365969201 2.393512909830744E-5 1.6754590368815207E-4 58-59 0.20213216523520633 2.393512909830744E-5 1.6754590368815207E-4 60-61 0.21774983697185193 2.393512909830744E-5 1.6754590368815207E-4 62-63 0.23523444877816552 2.393512909830744E-5 1.6754590368815207E-4 64-65 0.2539876224266894 2.393512909830744E-5 1.6754590368815207E-4 66-67 0.27355459046455577 2.393512909830744E-5 1.795134682373058E-4 68-69 0.2965263306166563 2.393512909830744E-5 1.795134682373058E-4 70-71 0.3207247461350451 2.393512909830744E-5 1.795134682373058E-4 72-73 0.3482321932512749 2.393512909830744E-5 1.9148103278645952E-4 74-75 0.3774569858803083 2.393512909830744E-5 1.9148103278645952E-4 76-77 0.4103199181322844 2.393512909830744E-5 1.9148103278645952E-4 78-79 0.4476527357433695 2.393512909830744E-5 1.9148103278645952E-4 80-81 0.48877328753426164 2.393512909830744E-5 1.9148103278645952E-4 82-83 0.5357100756960425 2.393512909830744E-5 1.9148103278645952E-4 84-85 0.5883254732363968 2.393512909830744E-5 1.9746481506103638E-4 86-87 0.6454287074826839 2.393512909830744E-5 2.0344859733561324E-4 88-89 0.7101193776531343 2.393512909830744E-5 2.0344859733561324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5955 0.0 85.500854 9 CGGGAGA 8630 0.0 83.19315 4 AGAGCGG 6320 0.0 83.115685 8 AAGCGGC 2360 0.0 78.8182 9 CGGAAAG 3390 0.0 77.45903 5 GAGAGCG 7580 0.0 76.251114 7 TCGGGAG 9190 0.0 74.40621 3 TCGGGAA 3320 0.0 71.65754 3 ATTCGGA 1055 0.0 71.133415 2 GAACCGG 1175 0.0 69.54299 1 GATCCGG 1665 0.0 67.05272 1 AACCGGA 1200 0.0 66.10042 2 GATCGGG 13495 0.0 65.76075 1 GTTCGGA 855 0.0 64.996315 2 CGGAGAG 3260 0.0 64.81702 5 ATCGGGA 13385 0.0 64.051285 2 ACCGGAA 1320 0.0 64.03366 3 AAGCGTC 1175 0.0 63.40365 9 TCGGAGA 1795 0.0 62.959187 4 ATCCGGA 1795 0.0 60.330845 2 >>END_MODULE