FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8355919
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC171590.20535144009892867No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG164470.1968305341399312No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA131310.1571460900949375No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA130540.15622458762465266No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC116430.13933835404579675No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG109770.1313679560560604No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC101630.12162635851304925No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA95390.11415859823437732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC59550.085.5008549
CGGGAGA86300.083.193154
AGAGCGG63200.083.1156858
AAGCGGC23600.078.81829
CGGAAAG33900.077.459035
GAGAGCG75800.076.2511147
TCGGGAG91900.074.406213
TCGGGAA33200.071.657543
ATTCGGA10550.071.1334152
GAACCGG11750.069.542991
GATCCGG16650.067.052721
AACCGGA12000.066.100422
GATCGGG134950.065.760751
GTTCGGA8550.064.9963152
CGGAGAG32600.064.817025
ATCGGGA133850.064.0512852
ACCGGAA13200.064.033663
AAGCGTC11750.063.403659
TCGGAGA17950.062.9591874
ATCCGGA17950.060.3308452
Produced by FastQC (version 0.11.2)