##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34298-1.342000000357ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5319197 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.704552021667933 33.0 33.0 33.0 27.0 33.0 2 31.5785017926578 33.0 33.0 33.0 27.0 33.0 3 31.52645258297446 33.0 33.0 33.0 27.0 33.0 4 35.1889253208708 37.0 37.0 37.0 33.0 37.0 5 35.16558627176245 37.0 37.0 37.0 33.0 37.0 6 35.33390265485561 37.0 37.0 37.0 33.0 37.0 7 35.134028688916764 37.0 37.0 37.0 33.0 37.0 8 35.14995139303921 37.0 37.0 37.0 33.0 37.0 9 34.99260734280005 37.0 37.0 37.0 33.0 37.0 10-11 34.939067024590365 37.0 37.0 37.0 33.0 37.0 12-13 34.924259338392616 37.0 37.0 37.0 33.0 37.0 14-15 36.516806860133215 40.0 37.0 40.0 33.0 40.0 16-17 36.4398177958064 40.0 37.0 40.0 33.0 40.0 18-19 36.42298047994838 40.0 37.0 40.0 33.0 40.0 20-21 36.38734728192996 40.0 37.0 40.0 33.0 40.0 22-23 36.55876206502598 40.0 37.0 40.0 33.0 40.0 24-25 36.23405694506144 40.0 37.0 40.0 33.0 40.0 26-27 36.09154859276691 38.5 37.0 40.0 27.0 40.0 28-29 35.99725738678225 37.0 37.0 40.0 27.0 40.0 30-31 35.97551726322601 37.0 37.0 40.0 27.0 40.0 32-33 35.853701507953176 37.0 37.0 40.0 27.0 40.0 34-35 35.82809971129101 37.0 37.0 40.0 27.0 40.0 36-37 35.72314486566299 37.0 37.0 40.0 27.0 40.0 38-39 35.71560754377023 37.0 37.0 40.0 27.0 40.0 40-41 35.495696060890396 37.0 37.0 40.0 27.0 40.0 42-43 35.37916061390469 37.0 37.0 40.0 27.0 40.0 44-45 35.264294309836615 37.0 37.0 40.0 27.0 40.0 46-47 35.15960190983714 37.0 37.0 40.0 27.0 40.0 48-49 35.02542254028192 37.0 37.0 40.0 27.0 40.0 50-51 33.89018276630853 37.0 33.0 38.5 24.5 38.5 52-53 34.37578266042788 37.0 33.0 38.5 27.0 40.0 54-55 35.135605618667626 37.0 37.0 40.0 27.0 40.0 56-57 35.21863676039823 37.0 37.0 40.0 27.0 40.0 58-59 34.881424489448314 37.0 33.0 40.0 27.0 40.0 60-61 34.6677412022905 37.0 33.0 40.0 27.0 40.0 62-63 34.4021616420674 37.0 33.0 40.0 27.0 40.0 64-65 34.145236207645624 37.0 33.0 37.0 27.0 40.0 66-67 33.90086849575228 37.0 33.0 37.0 27.0 40.0 68-69 33.73543403261808 37.0 33.0 37.0 27.0 40.0 70-71 33.371524874901226 37.0 33.0 37.0 22.0 40.0 72-73 33.24528166563487 37.0 33.0 37.0 22.0 37.0 74-75 33.04148821711247 37.0 33.0 37.0 24.5 37.0 76-77 32.75699884775841 37.0 33.0 37.0 22.0 37.0 78-79 32.44990437466407 37.0 33.0 37.0 22.0 37.0 80-81 32.180393769961896 35.0 33.0 37.0 22.0 37.0 82-83 31.968929614752 33.0 33.0 37.0 22.0 37.0 84-85 31.795908386171824 33.0 33.0 37.0 22.0 37.0 86-87 31.645261211419694 33.0 33.0 37.0 18.5 37.0 88-89 31.480834607178487 33.0 33.0 37.0 15.0 37.0 90-91 31.329187187464576 33.0 33.0 37.0 15.0 37.0 92-93 31.10574829245843 33.0 33.0 37.0 15.0 37.0 94-95 30.96179479722221 33.0 33.0 37.0 15.0 37.0 96-97 30.81968735130509 33.0 27.0 37.0 15.0 37.0 98-99 30.660277857729277 33.0 27.0 37.0 15.0 37.0 100-101 29.359490821640936 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21428008809821364 2306 2 0.2165482180321483 2306 3 0.24899941724340025 2306 4 0.31230901900705277 2306 5 0.3346365220014249 2306 6 0.17045837198354974 2306 7 0.42468697199564787 2306 8 0.24930257953796087 2306 9 0.3347190303626064 2306 10-11 0.3138667397101855 2306 12-13 0.26827726400136953 2306 14-15 0.5137394046083941 2306 16-17 0.4661160457525071 2306 18-19 0.4816402639556543 2306 20-21 0.5126786302165911 2306 22-23 0.5233963392607208 2306 24-25 0.4982381383447603 2306 26-27 0.4326265773191267 2306 28-29 0.5018188534169141 2306 30-31 0.5283178288483157 2306 32-33 0.5440196553212147 2306 34-35 0.3945421318251192 2306 36-37 0.4654786700447886 2306 38-39 0.41818951097511814 2306 40-41 0.44710053698727137 2306 42-43 0.4790126385356501 2306 44-45 0.5953388474630756 2306 46-47 0.5843575286038103 2306 48-49 0.4870972944638936 2306 50-51 0.5277598783817794 2306 52-53 0.5545772561302371 2306 54-55 0.48963046780378505 2306 56-57 0.391195720704566 2306 58-59 0.5597121067424524 2306 60-61 0.5775440113880066 2306 62-63 0.6018152638080991 2306 64-65 0.5201676885224202 2306 66-67 0.5002358277929417 2306 68-69 0.4510961857344782 2306 70-71 0.5492739687890307 2306 72-73 0.38777386889099574 2306 74-75 0.3626794613625819 2306 76-77 0.3566554923509173 2306 78-79 0.43043507554867944 2306 80-81 0.499035207696636 2306 82-83 0.49931358219240707 2306 84-85 0.5239134845682649 2306 86-87 0.5956225488029929 2306 88-89 0.6487051736468779 2306 90-91 0.4860775269208766 2306 92-93 0.5556618395416457 2306 94-95 0.46447119979955076 2306 96-97 0.2767474200915352 2306 98-99 0.29530404137298305 2306 100-101 0.31025897900320487 2307 1 0.24274261891483206 2307 2 0.25031995365698023 2307 3 0.32264498921626483 2307 4 0.38596989679557936 2307 5 0.45763979635270147 2307 6 0.2913961811746901 2307 7 0.45328698183510596 2307 8 0.3589140851382524 2307 9 0.40014319277917565 2307 10-11 0.4058617981182664 2307 12-13 0.4136672069872134 2307 14-15 0.5830218625037347 2307 16-17 0.56860337745686 2307 18-19 0.6118259289660131 2307 20-21 0.5574358417156233 2307 22-23 0.49940611311729555 2307 24-25 0.5597686105838307 2307 26-27 0.4690628914600552 2307 28-29 0.5041852418457111 2307 30-31 0.5096444604209367 2307 32-33 0.5027497099301712 2307 34-35 0.4648444763480981 2307 36-37 0.42031517113532857 2307 38-39 0.4068623874298538 2307 40-41 0.4588042929251017 2307 42-43 0.436801248822249 2307 44-45 0.4982528287790444 2307 46-47 0.4870635148176419 2307 48-49 0.4186051359612435 2307 50-51 0.2748732288794926 2307 52-53 0.38537542708527184 2307 54-55 0.36363217330895736 2307 56-57 0.34807623148149247 2307 58-59 0.34153645064226623 2307 60-61 0.3261312299386532 2307 62-63 0.4600148079134456 2307 64-65 0.44249463714407966 2307 66-67 0.43365511968031 2307 68-69 0.38870899779546875 2307 70-71 0.2876826832645065 2307 72-73 0.24158884344507925 2307 74-75 0.2007541810748279 2307 76-77 0.31500016838693057 2307 78-79 0.31544671890131326 2307 80-81 0.37783952284338085 2307 82-83 0.43621863693802965 2307 84-85 0.4890201879688938 2307 86-87 0.4844666590704563 2307 88-89 0.40744547768597883 2307 90-91 0.5404406409984013 2307 92-93 0.5441940938149763 2307 94-95 0.3540165077470938 2307 96-97 0.24821833630859302 2307 98-99 0.24891840041462032 2307 100-101 0.23518723299228128 2201 1 0.20867322106139596 2201 2 0.13158640725233894 2201 3 0.14759634380390096 2201 4 0.2859426025370624 2201 5 0.23818251186728645 2201 6 0.25699925221900344 2201 7 0.32109384413997333 2201 8 0.2564047742742588 2201 9 0.2026630691625968 2201 10-11 0.25054382000650577 2201 12-13 0.3189402816414031 2201 14-15 0.4966408170455878 2201 16-17 0.43170462154677836 2201 18-19 0.4032603743202685 2201 20-21 0.43898184134583573 2201 22-23 0.47467619420765317 2201 24-25 0.5001121660572068 2201 26-27 0.4320967803636222 2201 28-29 0.4157954270064437 2201 30-31 0.438704041145904 2201 32-33 0.40098394749588095 2201 34-35 0.3023670381343706 2201 36-37 0.2671217428884134 2201 38-39 0.32515614408833216 2201 40-41 0.38737829998639484 2201 42-43 0.37279973443112624 2201 44-45 0.4027370113159989 2201 46-47 0.3885382852377077 2201 48-49 0.4628420853435955 2201 50-51 0.3465008471472757 2201 52-53 0.4982631596367497 2201 54-55 0.5244103238147844 2201 56-57 0.5200833279887078 2201 58-59 0.5180510618751697 2201 60-61 0.5438945094758836 2201 62-63 0.5335415782982977 2201 64-65 0.5189850357282992 2201 66-67 0.5379433998778822 2201 68-69 0.512666753326954 2201 70-71 0.6533658761644787 2201 72-73 0.569499864162303 2201 74-75 0.4985878093622418 2201 76-77 0.6137692853736425 2201 78-79 0.5252554829996754 2201 80-81 0.6026046601511084 2201 82-83 0.6265916878198112 2201 84-85 0.6075512285845157 2201 86-87 0.6304570444477804 2201 88-89 0.5834947658341072 2201 90-91 0.5162544061092937 2201 92-93 0.6557364835593766 2201 94-95 0.5655007871346456 2201 96-97 0.5328296572626563 2201 98-99 0.4800444206475909 2201 100-101 0.3893592924761542 1101 1 0.11784165188480955 1101 2 0.11183160088068433 1101 3 0.0018994593048837771 1101 4 0.17707009238336013 1101 5 0.2292429810487846 1101 6 0.1625796727988842 1101 7 0.2560206280391455 1101 8 0.13495818093019096 1101 9 0.13209822128796844 1101 10-11 0.1682454834124698 1101 12-13 0.19535861664137144 1101 14-15 0.33375107641420954 1101 16-17 0.32027120350353755 1101 18-19 0.38439081377394757 1101 20-21 0.3424181969730782 1101 22-23 0.32369578427428536 1101 24-25 0.3492824605050231 1101 26-27 0.2734864429884567 1101 28-29 0.3091074637607605 1101 30-31 0.24374963449356102 1101 32-33 0.2681762509404706 1101 34-35 0.12476028867249767 1101 36-37 0.2627285295839954 1101 38-39 0.19571644925731135 1101 40-41 0.19642673453706294 1101 42-43 0.03140924762276853 1101 44-45 0.12476773745949998 1101 46-47 0.16466080622699764 1101 48-49 0.1512876723111063 1101 50-51 0.20525961997485354 1101 52-53 0.20953700605373626 1101 54-55 0.2955221968377728 1101 56-57 0.34671693002849935 1101 58-59 0.29411768382907155 1101 60-61 0.34126603308190084 1101 62-63 0.4273168546736983 1101 64-65 0.35971983606305 1101 66-67 0.38416765032418 1101 68-69 0.3366147291176773 1101 70-71 0.4516741085846192 1101 72-73 0.3354822855882773 1101 74-75 0.3601795918452808 1101 76-77 0.43619007333769133 1101 78-79 0.5209516693667737 1101 80-81 0.5281696161392304 1101 82-83 0.34735782555134165 1101 84-85 0.4018544623800686 1101 86-87 0.3980076447103329 1101 88-89 0.3549123382219044 1101 90-91 0.4334012243197165 1101 92-93 0.5250995409583332 1101 94-95 0.5791652408847163 1101 96-97 0.38972453905389415 1101 98-99 0.4257743112726189 1101 100-101 0.376171677375293 2304 1 0.1797507071802258 2304 2 0.15308860133464464 2304 3 0.16845939013320788 2304 4 0.15577338493528003 2304 5 0.24583728583603204 2304 6 0.2560375307760552 2304 7 0.3697522244469482 2304 8 0.3039899713198153 2304 9 0.22946321502852385 2304 10-11 0.24297210337262953 2304 12-13 0.27149734137425696 2304 14-15 0.48109165900884676 2304 16-17 0.3481003831853471 2304 18-19 0.34793284625091303 2304 20-21 0.4639000729933045 2304 22-23 0.26601474846631135 2304 24-25 0.28677171441654536 2304 26-27 0.37784271678819437 2304 28-29 0.3545323461792904 2304 30-31 0.3351146706098902 2304 32-33 0.3058541446304943 2304 34-35 0.3269304012252334 2304 36-37 0.31886571256029583 2304 38-39 0.29341188380951877 2304 40-41 0.40005396484348665 2304 42-43 0.23487224929660755 2304 44-45 0.2872337410506205 2304 46-47 0.2879226672714523 2304 48-49 0.2765940738918147 2304 50-51 0.31929066428052266 2304 52-53 0.2775113407654928 2304 54-55 0.3723744347966331 2304 56-57 0.4850622871096917 2304 58-59 0.5383158329097171 2304 60-61 0.5539606178647674 2304 62-63 0.5306673351652975 2304 64-65 0.4860318506295016 2304 66-67 0.37301407829507127 2304 68-69 0.45168959221916083 2304 70-71 0.4853609489708859 2304 72-73 0.4287498358046875 2304 74-75 0.4401292728774777 2304 76-77 0.4291920762688406 2304 78-79 0.5049190914190334 2304 80-81 0.46558058758562737 2304 82-83 0.5116318993816549 2304 84-85 0.4663985690155279 2304 86-87 0.4128674508006327 2304 88-89 0.3160544597781403 2304 90-91 0.378686289251565 2304 92-93 0.44240282837106193 2304 94-95 0.4371715332351229 2304 96-97 0.42504289150215513 2304 98-99 0.4013781095838098 2304 100-101 0.37076813611369275 2202 1 0.11778745841004934 2202 2 0.0694930038425916 2202 3 0.06006325650275812 2202 4 0.08352933282719732 2202 5 0.22905350231725663 2202 6 0.15684698209110337 2202 7 0.19690983404389328 2202 8 0.04458859951150629 2202 9 0.11545169674990063 2202 10-11 0.07902666449245288 2202 12-13 0.03434731172379202 2202 14-15 0.17468055050775888 2202 16-17 0.1398254306452813 2202 18-19 0.1477672427520389 2202 20-21 0.2464859133292734 2202 22-23 0.2984939664955064 2202 24-25 0.2659642923785128 2202 26-27 0.24272701602470192 2202 28-29 0.19879369147910353 2202 30-31 0.3123879191541121 2202 32-33 0.22981161422438134 2202 34-35 0.213316659609319 2202 36-37 0.18587250339086125 2202 38-39 0.16365428435896234 2202 40-41 0.22519559857347815 2202 42-43 0.23524711132921539 2202 44-45 0.19142507203039116 2202 46-47 0.16993790134386444 2202 48-49 0.18633916515172189 2202 50-51 0.22691646133662147 2202 52-53 0.2883021065319227 2202 54-55 0.24223647380223667 2202 56-57 0.4425281933374805 2202 58-59 0.4963408774280538 2202 60-61 0.2983758002673511 2202 62-63 0.4165709368335584 2202 64-65 0.4079481702372192 2202 66-67 0.4016186988223964 2202 68-69 0.40599295975319905 2202 70-71 0.4276923495173435 2202 72-73 0.39326688279737 2202 74-75 0.38138123447640027 2202 76-77 0.49361718763393725 2202 78-79 0.4218064490658904 2202 80-81 0.3268357423116015 2202 82-83 0.40999939718504663 2202 84-85 0.4383807761782812 2202 86-87 0.38079581476425517 2202 88-89 0.3646932241701286 2202 90-91 0.36673932585571123 2202 92-93 0.4985781186834366 2202 94-95 0.4985037876979348 2202 96-97 0.3869754879578231 2202 98-99 0.5248361468210412 2202 100-101 0.4611744732558982 1102 1 0.11105904861456395 1102 2 0.07121808309045363 1102 3 0.07223114243636886 1102 4 0.1038493558885989 1102 5 0.16180252673980533 1102 6 0.1319861896828627 1102 7 0.07258912565787057 1102 8 0.1200438851043657 1102 9 0.11813020671691987 1102 10-11 0.1330729414323102 1102 12-13 0.15352057636117422 1102 14-15 0.09939011560435773 1102 16-17 0.0642219584161765 1102 18-19 0.03515568565919125 1102 20-21 0.03804849995406556 1102 22-23 0.06607872923085978 1102 24-25 0.13183423763113922 1102 26-27 0.17164120976987363 1102 28-29 0.11392130253565114 1102 30-31 0.13452437339167744 1102 32-33 0.1299773536633424 1102 34-35 0.16532535898572576 1102 36-37 0.14151816324231703 1102 38-39 0.1439758273466012 1102 40-41 0.14380692253416072 1102 42-43 0.2446560060906222 1102 44-45 0.2215574862431211 1102 46-47 0.223442802283067 1102 48-49 0.12950986788636953 1102 50-51 0.26361761902747816 1102 52-53 0.2115667747767489 1102 54-55 0.3062139915668354 1102 56-57 0.3985829454090819 1102 58-59 0.38805740698796853 1102 60-61 0.34428452626921313 1102 62-63 0.30902286546319147 1102 64-65 0.33823439160389057 1102 66-67 0.429601594179843 1102 68-69 0.3943384295233514 1102 70-71 0.4083560816373861 1102 72-73 0.4097108470793529 1102 74-75 0.40989766755761536 1102 76-77 0.4310567727261656 1102 78-79 0.33268201092735694 1102 80-81 0.2571070483220055 1102 82-83 0.3945324195986899 1102 84-85 0.29455187214343326 1102 86-87 0.2227475152609415 1102 88-89 0.21806266075766345 1102 90-91 0.4104508038621866 1102 92-93 0.3841053308560447 1102 94-95 0.4094175945901952 1102 96-97 0.28561395663255595 1102 98-99 0.48580813183195204 1102 100-101 0.35148060672607784 2305 1 0.18774338059575513 2305 2 0.11454249505977998 2305 3 0.14688102325251862 2305 4 0.14372903152605687 2305 5 0.16632926992237174 2305 6 0.17857426217401695 2305 7 0.10057626824889354 2305 8 0.1871108599636102 2305 9 0.13938325284315312 2305 10-11 0.1644122105140795 2305 12-13 0.129129003248444 2305 14-15 0.16265919357378777 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80-81 -0.32349645560955764 1213 82-83 -0.2902071459839881 1213 84-85 -0.3460978257292062 1213 86-87 -0.3410094695795749 1213 88-89 -0.42410464690333427 1213 90-91 -0.3784149824046743 1213 92-93 -0.38680800237426993 1213 94-95 -0.3354082198804349 1213 96-97 -0.31683518084124884 1213 98-99 -0.41661552970048277 1213 100-101 -0.16688960364974648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 770.0 3 385.0 4 255.0 5 320.0 6 797.0 7 3969.0 8 16628.0 9 41772.0 10 46282.0 11 34682.0 12 23225.0 13 17457.0 14 15670.0 15 16203.0 16 16470.0 17 16807.0 18 17608.0 19 19153.0 20 21149.0 21 23766.0 22 26936.0 23 29311.0 24 33174.0 25 38240.0 26 44329.0 27 52981.0 28 64469.0 29 78070.0 30 96622.0 31 126147.0 32 172587.0 33 244500.0 34 366435.0 35 608817.0 36 1117746.0 37 1565858.0 38 319547.0 39 60.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.85478816727159 24.053938665878864 26.389370380195587 13.701902786653966 2 31.563177990902382 22.21094664083853 34.341159323588194 11.884716044670894 3 38.55252393306877 18.236196111045825 27.672507179167305 15.538772776718105 4 29.747962738502746 16.967836340448113 36.652893799159166 16.63130712188997 5 30.909732569403747 16.130542469352672 37.121927169568565 15.837797791675015 6 24.89196485045631 16.817904581187314 43.101902051143036 15.188228517213338 7 34.24065594048923 17.339915274478955 31.87101784498584 16.548410940045972 8 29.609097003764635 28.23762773534415 25.99783856383069 16.15543669706053 9 30.83838026088328 26.84245945552274 24.434481276466492 17.884679007127488 10-11 25.56185018432299 27.270544599539683 28.898658860223115 18.268946355914217 12-13 26.807879442519333 24.110263335063014 28.490771048331894 20.591086174085756 14-15 24.905852134753854 25.026981730340793 29.849147919355467 20.21801821554989 16-17 23.792437970298007 26.66432684047247 31.147618881832816 18.395616307396708 18-19 22.8689460922379 27.175515304816255 31.091740642760037 18.86379796018581 20-21 22.8188041340634 28.06208980007992 30.0869776965283 19.032128369328383 22-23 23.669056511149435 27.375534172907052 30.147028731853315 18.808380584090198 24-25 23.018216992478067 27.998488421598246 30.231888924658634 18.751405661265057 26-27 22.13417900529509 28.60534760123803 30.184088669356907 19.076384724109978 28-29 21.98280412253963 28.355679029606364 29.76198921513043 19.899527632723576 30-31 22.350564006506055 27.99137297124848 29.50949311215335 20.148569910092114 32-33 21.222692105681578 27.977738852471244 30.736692405596504 20.06287663625067 34-35 20.634507228253355 28.139747236256067 32.11398670509276 19.11175883039781 36-37 20.682540558738747 29.061276179986923 30.839571967846535 19.416611293427803 38-39 20.781479008907265 28.809387062597846 29.33560681770247 21.073527110792423 40-41 21.45933472267175 27.947207275300386 29.927607957887297 20.665850044140573 42-43 21.757033115318038 28.210402610869977 31.10124149350792 18.931322780304065 44-45 20.916799104172366 29.25653766537733 31.238861497120286 18.587801733330018 46-47 21.812940712503625 28.98766443076191 30.54399206507872 18.655402791655746 48-49 21.40753672970904 29.45765008803675 29.940333210462143 19.194479971792067 50-51 20.453537668994205 29.511262417825655 29.95217277151766 20.083027141662484 52-53 21.721203765577734 28.977998585901936 29.413823759974843 19.886973888545487 54-55 22.718093047169102 28.802063775997688 29.059357670346426 19.420485506486784 56-57 22.992594406598865 28.546285547437005 28.348865849264975 20.112254196699155 58-59 22.187912968794194 28.26754825698266 29.14188952010171 20.402649254121435 60-61 20.663245920722325 28.345281712468445 29.409623293891897 21.581849072917333 62-63 21.776105319313004 28.316871227623725 29.07916592834658 20.8278575247167 64-65 21.0378879582753 28.66188056425364 29.6015280676791 20.698703409791957 66-67 21.181039580087038 28.98642669523643 28.730634166397262 21.10189955827927 68-69 21.549091640478444 28.523922133456068 30.009000072881946 19.917986153183538 70-71 20.911111843437077 30.543968589493108 29.229077316360108 19.315842250709704 72-73 20.401839229469328 31.763863545692654 28.3184767460331 19.51582047880492 74-75 21.541748086525438 31.203915496896123 27.646507494079163 19.60782892249928 76-77 22.034140247721076 30.016202065280755 28.24289181112574 19.706765875872424 78-79 21.891812213824267 29.904266687230784 28.97994537138335 19.223975727561594 80-81 20.64506427167789 30.76699547573264 28.805807921229704 19.782132331359765 82-83 19.76600068285906 30.375228698918022 29.0600704051238 20.79870021309912 84-85 19.555913880257805 29.475912448633412 29.857412094023815 21.11076157708497 86-87 19.817013124542765 29.48248097100951 30.535601644446125 20.1649042600016 88-89 20.04656875466213 30.066349030546064 29.967009638445496 19.920072576346318 90-91 20.377624394231105 29.710955341705127 29.398432885981574 20.512987378082194 92-93 19.75855870039125 30.564247501766427 29.227317786760153 20.449876011082168 94-95 19.547614965102827 31.04070252531002 29.35612988869043 20.055552620896723 96-97 20.372984255427543 31.35946489453316 28.508547948224045 19.759002901815247 98-99 20.743531564904647 30.464373348437817 28.17059676109448 20.62149832556306 100-101 20.53251118225067 30.364009269451408 28.154639934636844 20.94883961366108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2939.0 1 1998.0 2 1003.5 3 1175.5 4 1277.0 5 1137.0 6 1304.5 7 1701.5 8 2049.5 9 2198.5 10 2188.5 11 2183.5 12 2161.0 13 2052.5 14 1891.0 15 1815.5 16 1773.0 17 1703.5 18 1774.0 19 1910.0 20 2172.0 21 2591.5 22 3077.0 23 3853.5 24 4937.0 25 6323.0 26 8023.5 27 10110.5 28 12816.0 29 15592.5 30 19041.5 31 24595.5 32 35933.0 33 56540.0 34 85945.5 35 126869.5 36 178515.5 37 264076.0 38 372745.0 39 434295.5 40 424721.0 41 364766.5 42 317869.5 43 312610.5 44 308712.0 45 300820.0 46 299633.0 47 270698.0 48 214176.5 49 170909.5 50 141860.5 51 119385.0 52 98523.5 53 72970.5 54 50470.5 55 37280.0 56 27876.5 57 20624.5 58 15477.5 59 11533.5 60 8602.5 61 6682.5 62 5078.0 63 3696.5 64 2846.0 65 2140.5 66 1581.5 67 1197.5 68 903.5 69 679.5 70 489.5 71 343.0 72 261.5 73 199.5 74 151.0 75 123.0 76 88.0 77 68.0 78 60.5 79 56.0 80 48.0 81 32.5 82 22.0 83 18.5 84 16.5 85 14.5 86 10.0 87 10.0 88 10.5 89 6.5 90 4.5 91 4.5 92 3.5 93 2.0 94 2.0 95 2.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21832242723854745 2 0.20254936976389482 3 0.17651160504113683 4 0.16006175368199374 5 0.17124765260621105 6 0.13229440458776012 7 0.11798773386283681 8 0.11398337004626827 9 0.10157548216394316 10-11 0.08672361636540252 12-13 0.11430296715838875 14-15 0.11053360121837938 16-17 0.10174468063506578 18-19 0.09468534442322779 20-21 0.0986709084096716 22-23 0.08564262613323026 24-25 0.40275816067726017 26-27 0.1903482800129418 28-29 0.09211916761120147 30-31 0.17622020767420346 32-33 0.2072681271252033 34-35 0.2067793315419602 36-37 0.21202448414676126 38-39 0.21316187386930774 40-41 0.20971210504141885 42-43 0.2256073614118823 44-45 0.20741852576620115 46-47 0.22604915741981355 48-49 0.21564345144577274 50-51 0.2191214200188487 52-53 0.2109340939995266 54-55 0.19340325240821124 56-57 0.2013367807208494 58-59 0.1965992235294162 60-61 0.17561861311021193 62-63 0.16389691902743966 64-65 0.16846527774775027 66-67 0.180863765715013 68-69 0.17371043035255132 70-71 0.16679209286664887 72-73 0.16992226458241724 74-75 0.15988315529580877 76-77 0.17054265897653348 78-79 0.16875667511468367 80-81 0.17439662415210416 82-83 0.19992679346149425 84-85 0.19818780917495632 86-87 0.2027279681500798 88-89 0.2092703090334876 90-91 0.19060207771962573 92-93 0.17004446347822802 94-95 0.17101265472965185 96-97 0.1802809709811462 98-99 0.1954712337219321 100-101 0.2069485300130828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5319197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.991220353802234 #Duplication Level Percentage of deduplicated Percentage of total 1 87.59100551463615 33.276891915176485 2 5.184028143865758 3.9389511106783277 3 2.0309627074468732 2.3147625524690687 4 1.1904349395603715 1.8090430442281327 5 0.6466181203439644 1.2282905747374482 6 0.4928386094027672 1.12341241252092 7 0.2871935424286984 0.763758320821841 8 0.25501344342249027 0.7750617537796567 9 0.22066624902778997 0.7545042082315591 >10 1.6492987567355868 12.504636350119283 >50 0.20213867531440402 5.388295848169211 >100 0.20053552635830244 16.48301288479018 >500 0.03479406011817991 9.104315980887531 >1k 0.01377241149658813 8.457267789110896 >5k 5.99401675592181E-4 1.5470101098952542 >10k+ 9.989816653853951E-5 0.5307851443842273 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 14545 0.27344352916427045 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13426 0.252406519254692 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9633 0.18109876359157218 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 9356 0.1758912106470206 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7475 0.14052873018239406 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7441 0.1398895359581531 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6927 0.1302264232740393 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 6144 0.11550615628637181 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 6026 0.11328777633165307 Illumina Single End PCR Primer 1 (96% over 32bp) TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 5995 0.11270498159778627 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 5849 0.10996020639957498 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5801 0.1090578145535877 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG 5798 0.10900141506321348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016355852208519443 0.0 0.0 2 0.0018235835220992943 0.0 0.0 3 0.002011581823346644 0.0 0.0 4 0.002161980464344524 0.0 0.0 5 0.0023311789354671392 0.0 0.0 6 0.002669575877712369 0.0 0.0 7 0.0030831721404565387 0.0 0.0 8 0.0037035665345727937 0.0 0.0 9 0.004098362967192229 0.0 0.0 10-11 0.006880737825653007 0.0 0.0 12-13 0.010339906568604246 0.0 0.0 14-15 0.014739066817792235 0.0 9.399915062367496E-6 16-17 0.0196646223104728 0.0 1.879983012473499E-5 18-19 0.0253327710930804 0.0 1.879983012473499E-5 20-21 0.030220726925511504 0.0 1.879983012473499E-5 22-23 0.035418879955000725 0.0 1.879983012473499E-5 24-25 0.04191422126309667 9.399915062367496E-6 1.879983012473499E-5 26-27 0.04925555492680568 1.879983012473499E-5 1.879983012473499E-5 28-29 0.056719087486325474 1.879983012473499E-5 1.879983012473499E-5 30-31 0.0841292398081891 1.879983012473499E-5 1.879983012473499E-5 32-33 0.09339755605968345 1.879983012473499E-5 1.879983012473499E-5 34-35 0.10201727817187445 1.879983012473499E-5 1.879983012473499E-5 36-37 0.11137959357399246 1.879983012473499E-5 1.879983012473499E-5 38-39 0.1221518962354656 1.879983012473499E-5 1.879983012473499E-5 40-41 0.13408038844960996 1.879983012473499E-5 1.879983012473499E-5 42-43 0.14637547735118667 1.879983012473499E-5 2.8199745187102487E-5 44-45 0.1597233567397485 1.879983012473499E-5 5.639949037420498E-5 46-47 0.17375742992786317 1.879983012473499E-5 5.639949037420498E-5 48-49 0.18854349632096723 1.879983012473499E-5 5.639949037420498E-5 50-51 0.20464555082280278 1.879983012473499E-5 7.519932049893996E-5 52-53 0.2218943949622471 1.879983012473499E-5 7.519932049893996E-5 54-55 0.23923723825231516 1.879983012473499E-5 7.519932049893996E-5 56-57 0.2585070641301685 1.879983012473499E-5 8.459923556130747E-5 58-59 0.27877328100463283 1.879983012473499E-5 9.399915062367497E-5 60-61 0.3002332870920178 1.879983012473499E-5 9.399915062367497E-5 62-63 0.32341347763581607 1.879983012473499E-5 9.399915062367497E-5 64-65 0.34705426401767037 1.879983012473499E-5 9.399915062367497E-5 66-67 0.3722084367245657 1.879983012473499E-5 9.399915062367497E-5 68-69 0.3996467887916165 1.879983012473499E-5 9.399915062367497E-5 70-71 0.4286831264192697 1.879983012473499E-5 9.399915062367497E-5 72-73 0.458565456402536 1.879983012473499E-5 9.399915062367497E-5 74-75 0.48967917525897237 1.879983012473499E-5 9.399915062367497E-5 76-77 0.522691676958007 1.879983012473499E-5 9.399915062367497E-5 78-79 0.5595299440874253 1.879983012473499E-5 9.399915062367497E-5 80-81 0.5986147909167492 1.879983012473499E-5 9.399915062367497E-5 82-83 0.640200015152663 1.879983012473499E-5 9.399915062367497E-5 84-85 0.6866355955607585 1.879983012473499E-5 9.399915062367497E-5 86-87 0.7360509490436244 1.879983012473499E-5 9.399915062367497E-5 88-89 0.7906832553861043 1.879983012473499E-5 9.399915062367497E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 1960 0.0 78.00846 4 GAGCGGC 1430 0.0 75.65366 9 CGGAAAG 875 0.0 72.17479 5 AGAGCGG 1570 0.0 71.62751 8 GAGAGCG 1825 0.0 68.64114 7 AAGCGGC 570 0.0 68.26061 9 TCGGGAA 840 0.0 61.05183 3 TCGGGAG 2405 0.0 60.61463 3 GAACCGG 315 0.0 60.322536 1 CGATCGC 50 4.2424472E-7 57.00479 1 GGATCGG 805 0.0 53.700165 1 ATTCGGA 300 0.0 52.243565 2 ACCGGAA 410 0.0 50.959248 3 AACCGGA 350 0.0 50.2081 2 GGAAAGC 1045 0.0 49.045586 6 AAGCGTC 370 0.0 46.16703 9 GATCCGG 620 0.0 45.205414 1 AAAGCGG 940 0.0 44.42076 8 TCGGAAA 885 0.0 43.99593 4 TTGTGTT 71500 0.0 42.60743 1 >>END_MODULE