FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319197
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC145450.27344352916427045No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG134260.252406519254692No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA96330.18109876359157218No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA93560.1758912106470206No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74750.14052873018239406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74410.1398895359581531No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA69270.1302264232740393No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT61440.11550615628637181No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT60260.11328777633165307Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC59950.11270498159778627No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT58490.10996020639957498No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT58010.1090578145535877No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG57980.10900141506321348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA19600.078.008464
GAGCGGC14300.075.653669
CGGAAAG8750.072.174795
AGAGCGG15700.071.627518
GAGAGCG18250.068.641147
AAGCGGC5700.068.260619
TCGGGAA8400.061.051833
TCGGGAG24050.060.614633
GAACCGG3150.060.3225361
CGATCGC504.2424472E-757.004791
GGATCGG8050.053.7001651
ATTCGGA3000.052.2435652
ACCGGAA4100.050.9592483
AACCGGA3500.050.20812
GGAAAGC10450.049.0455866
AAGCGTC3700.046.167039
GATCCGG6200.045.2054141
AAAGCGG9400.044.420768
TCGGAAA8850.043.995934
TTGTGTT715000.042.607431
Produced by FastQC (version 0.11.2)