##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4624694 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28514297378378 33.0 33.0 33.0 33.0 33.0 2 32.276970541186074 33.0 33.0 33.0 33.0 33.0 3 32.26427283621359 33.0 33.0 33.0 33.0 33.0 4 36.070198157975426 37.0 37.0 37.0 37.0 37.0 5 36.023153748118254 37.0 37.0 37.0 37.0 37.0 6 36.060742613457236 37.0 37.0 37.0 33.0 37.0 7 35.99673297303562 37.0 37.0 37.0 33.0 37.0 8 35.95393295210451 37.0 37.0 37.0 33.0 37.0 9 35.9364336321495 37.0 37.0 37.0 33.0 37.0 10-11 35.87684796010287 37.0 37.0 37.0 33.0 37.0 12-13 35.889817250611614 37.0 37.0 37.0 33.0 37.0 14-15 37.7588562616251 40.0 37.0 40.0 33.0 40.0 16-17 37.72365209460345 40.0 37.0 40.0 33.0 40.0 18-19 37.67162497670116 40.0 37.0 40.0 33.0 40.0 20-21 37.62442628636619 40.0 37.0 40.0 33.0 40.0 22-23 37.64514765733689 40.0 37.0 40.0 33.0 40.0 24-25 37.466536164338656 40.0 37.0 40.0 33.0 40.0 26-27 37.385182889938235 40.0 37.0 40.0 33.0 40.0 28-29 37.33749746469712 40.0 37.0 40.0 33.0 40.0 30-31 37.31812558841731 40.0 37.0 40.0 33.0 40.0 32-33 37.27060676879378 40.0 37.0 40.0 33.0 40.0 34-35 37.217865441475695 40.0 37.0 40.0 33.0 40.0 36-37 37.1378356059882 40.0 37.0 40.0 33.0 40.0 38-39 37.10326953523844 40.0 37.0 40.0 33.0 40.0 40-41 36.974765141217986 40.0 37.0 40.0 33.0 40.0 42-43 36.89515836074776 40.0 37.0 40.0 33.0 40.0 44-45 36.7813871577233 40.0 37.0 40.0 33.0 40.0 46-47 36.68286993690826 40.0 37.0 40.0 33.0 40.0 48-49 36.56302298054747 38.5 37.0 40.0 33.0 40.0 50-51 35.403299007458656 37.0 35.0 38.5 30.0 38.5 52-53 35.81264738812989 37.0 37.0 38.5 33.0 40.0 54-55 36.59071497487185 37.0 37.0 40.0 33.0 40.0 56-57 36.61946747179381 37.0 37.0 40.0 33.0 40.0 58-59 36.44307763929895 37.0 37.0 40.0 33.0 40.0 60-61 36.229148133908964 37.0 37.0 40.0 33.0 40.0 62-63 35.98951692803891 37.0 37.0 40.0 33.0 40.0 64-65 35.73761345074939 37.0 37.0 40.0 33.0 40.0 66-67 35.476224372899054 37.0 37.0 40.0 30.0 40.0 68-69 35.25147966546544 37.0 35.0 37.0 27.0 40.0 70-71 34.92831450037559 37.0 33.0 37.0 27.0 40.0 72-73 34.69141547527252 37.0 33.0 37.0 27.0 40.0 74-75 34.42418363247385 37.0 33.0 37.0 27.0 38.5 76-77 34.16743594278886 37.0 33.0 37.0 27.0 37.0 78-79 33.903584539863616 37.0 33.0 37.0 27.0 37.0 80-81 33.683392566081125 37.0 33.0 37.0 27.0 37.0 82-83 33.45737415275475 37.0 33.0 37.0 27.0 37.0 84-85 33.274877105382544 37.0 33.0 37.0 27.0 37.0 86-87 33.113420044655925 37.0 33.0 37.0 27.0 37.0 88-89 32.93157514853955 37.0 33.0 37.0 27.0 37.0 90-91 32.78768292561627 37.0 33.0 37.0 27.0 37.0 92-93 32.6154143387649 33.0 33.0 37.0 27.0 37.0 94-95 32.47881686874851 33.0 33.0 37.0 24.5 37.0 96-97 32.34537906724207 33.0 33.0 37.0 22.0 37.0 98-99 32.18814055589408 33.0 33.0 37.0 22.0 37.0 100-101 30.885252516166474 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.18878362693535422 2306 2 0.15090438936825024 2306 3 0.16757454447590447 2306 4 0.18578724489151455 2306 5 0.31253692703256064 2306 6 0.24875860578409004 2306 7 0.2981325603169793 2306 8 0.192658238965997 2306 9 0.24498971359918897 2306 10-11 0.16025509267711158 2306 12-13 0.1899104183175453 2306 14-15 0.385148922260548 2306 16-17 0.3797236103201058 2306 18-19 0.3000336195874169 2306 20-21 0.28720049380941504 2306 22-23 0.2990621134426803 2306 24-25 0.2872510791721581 2306 26-27 0.25791499855969136 2306 28-29 0.24315490898789704 2306 30-31 0.22579507191350245 2306 32-33 0.19460890312853252 2306 34-35 0.16017273426390943 2306 36-37 0.16828016171776738 2306 38-39 0.18202386915893953 2306 40-41 0.14291241853636194 2306 42-43 0.1223396696617911 2306 44-45 0.26023773072254386 2306 46-47 0.21765613430190456 2306 48-49 0.23101456509248663 2306 50-51 0.2645715158746853 2306 52-53 0.2584357272532358 2306 54-55 0.22240035219507348 2306 56-57 0.1923293714403158 2306 58-59 0.3376954374142045 2306 60-61 0.3047444353017852 2306 62-63 0.3573748279445681 2306 64-65 0.27953270229115645 2306 66-67 0.17039940699145717 2306 68-69 0.25014697215043213 2306 70-71 0.38112552508459174 2306 72-73 0.21397315677807427 2306 74-75 0.20551601106331674 2306 76-77 0.2351541786948772 2306 78-79 0.16846462656555872 2306 80-81 0.27823637567367854 2306 82-83 0.2527633337868451 2306 84-85 0.274534417765004 2306 86-87 0.25052862361421546 2306 88-89 0.1611296931727182 2306 90-91 0.14434937920815116 2306 92-93 0.18181102386098758 2306 94-95 0.16650461418664975 2306 96-97 0.04762738823517765 2306 98-99 0.05547017399344156 2306 100-101 0.22903293051941276 2307 1 0.24493738721405123 2307 2 0.24727409976783576 2307 3 0.22752914769565535 2307 4 0.29510998640743225 2307 5 0.33020435681168436 2307 6 0.25789970665410067 2307 7 0.3173306117039587 2307 8 0.2871775163489687 2307 9 0.287299635131653 2307 10-11 0.3282211724047812 2307 12-13 0.34406448751150265 2307 14-15 0.46085294702881896 2307 16-17 0.47538306412121045 2307 18-19 0.5097974064603505 2307 20-21 0.5527923991589958 2307 22-23 0.47126946975502193 2307 24-25 0.4359954036188185 2307 26-27 0.3867868576025799 2307 28-29 0.4014102297474622 2307 30-31 0.4764410986056191 2307 32-33 0.4739434277736976 2307 34-35 0.4393178189173952 2307 36-37 0.42806050308443133 2307 38-39 0.332623883534211 2307 40-41 0.424804860970319 2307 42-43 0.3556392249461524 2307 44-45 0.46944514514766666 2307 46-47 0.4316862397765888 2307 48-49 0.3687447987211243 2307 50-51 0.14978474004776388 2307 52-53 0.35951285895971097 2307 54-55 0.367004428347272 2307 56-57 0.29241801253429855 2307 58-59 0.3003899049253391 2307 60-61 0.42925468226577834 2307 62-63 0.3970525658590205 2307 64-65 0.3764118552516251 2307 66-67 0.38655817949677385 2307 68-69 0.35183094265973125 2307 70-71 0.2474600313783597 2307 72-73 0.27255895569870603 2307 74-75 0.24908711356965796 2307 76-77 0.25546449707641017 2307 78-79 0.265674295919176 2307 80-81 0.2502764857209172 2307 82-83 0.39526693907266264 2307 84-85 0.45902574366908055 2307 86-87 0.33577126737833396 2307 88-89 0.3330001292173179 2307 90-91 0.44530214193522966 2307 92-93 0.3706918712675531 2307 94-95 0.257678679526812 2307 96-97 0.2716074899631167 2307 98-99 0.3120102503964475 2307 100-101 0.11885245212895512 2201 1 0.19475517015870736 2201 2 0.15022525373859708 2201 3 0.13938389709161925 2201 4 0.2421066342912397 2201 5 0.24558777495376916 2201 6 0.231392736666443 2201 7 0.19653928570166102 2201 8 0.21773757174464237 2201 9 0.21171079134704485 2201 10-11 0.1769162981779573 2201 12-13 0.1997440268972781 2201 14-15 0.29130995608567645 2201 16-17 0.2598572657436975 2201 18-19 0.32085123725365605 2201 20-21 0.3762389220001907 2201 22-23 0.30864855640394495 2201 24-25 0.34895062862141657 2201 26-27 0.4108963392421643 2201 28-29 0.4065168865462425 2201 30-31 0.39400230701915717 2201 32-33 0.3650120921576274 2201 34-35 0.3024914449838576 2201 36-37 0.25310592500002116 2201 38-39 0.31378543520614244 2201 40-41 0.2809762064119212 2201 42-43 0.22858345975098615 2201 44-45 0.3214315069263378 2201 46-47 0.24821240555282031 2201 48-49 0.24476348311610252 2201 50-51 0.22024389844001746 2201 52-53 0.39060979822139785 2201 54-55 0.39360589497751874 2201 56-57 0.34535479348631526 2201 58-59 0.45956910232326464 2201 60-61 0.43057101785009166 2201 62-63 0.47150334713715125 2201 64-65 0.5051917209552172 2201 66-67 0.32047921841985527 2201 68-69 0.35366952439481736 2201 70-71 0.4104320646072992 2201 72-73 0.3620222624141576 2201 74-75 0.38252629902448376 2201 76-77 0.290840519598909 2201 78-79 0.3524836233995359 2201 80-81 0.43958528910226136 2201 82-83 0.43955754192418084 2201 84-85 0.40182443776253507 2201 86-87 0.43908230268037585 2201 88-89 0.4851143129251483 2201 90-91 0.428678276689034 2201 92-93 0.4960989744504758 2201 94-95 0.27027624664854955 2201 96-97 0.31543024179004675 2201 98-99 0.17898610901887935 2201 100-101 0.04019233511193221 1101 1 0.27100514358506445 1101 2 0.2883171557668689 1101 3 0.16210239767074341 1101 4 0.3089056881969654 1101 5 0.3655995797116063 1101 6 0.3599283946091205 1101 7 0.4253008458405745 1101 8 0.37108132760246804 1101 9 0.3749118918311254 1101 10-11 0.33344254529396267 1101 12-13 0.32410437012118365 1101 14-15 0.481399092353918 1101 16-17 0.4183348060279215 1101 18-19 0.4456627058782132 1101 20-21 0.4687111821529939 1101 22-23 0.4756174148481307 1101 24-25 0.4996296649024572 1101 26-27 0.5875316566946864 1101 28-29 0.6046665044961372 1101 30-31 0.5296126151820246 1101 32-33 0.4018522889262286 1101 34-35 0.3278297223476514 1101 36-37 0.4406012720873136 1101 38-39 0.35519674234380716 1101 40-41 0.4081949031307701 1101 42-43 0.363293942083331 1101 44-45 0.4264011369962404 1101 46-47 0.3911934932863943 1101 48-49 0.3891268850365037 1101 50-51 0.35624050445088784 1101 52-53 0.46280372507603573 1101 54-55 0.4438198056931597 1101 56-57 0.43910731697155114 1101 58-59 0.47593923143598715 1101 60-61 0.4903616636545749 1101 62-63 0.565021578027185 1101 64-65 0.5109374467623056 1101 66-67 0.5113945018225579 1101 68-69 0.45027397520239276 1101 70-71 0.5309680672191561 1101 72-73 0.4677200707983076 1101 74-75 0.4218861212838192 1101 76-77 0.3826478123429169 1101 78-79 0.48672228724709754 1101 80-81 0.5566095702540181 1101 82-83 0.4519637329392907 1101 84-85 0.3770245847481064 1101 86-87 0.4931319917869956 1101 88-89 0.5333749904959362 1101 90-91 0.6279005400549238 1101 92-93 0.6282218916935776 1101 94-95 0.5274978298909687 1101 96-97 0.4492470011350562 1101 98-99 0.42903571987977074 1101 100-101 0.4434202379899155 2304 1 0.319942066233331 2304 2 0.26159253971871266 2304 3 0.24316123833274617 2304 4 0.317311612398683 2304 5 0.34452087387792574 2304 6 0.3677775009259534 2304 7 0.32405269590579877 2304 8 0.3899377531458015 2304 9 0.3418409758693741 2304 10-11 0.37457278724330223 2304 12-13 0.3660122927309786 2304 14-15 0.47903302004716153 2304 16-17 0.4768003903373099 2304 18-19 0.394295815593793 2304 20-21 0.5383696427166953 2304 22-23 0.42186944180910046 2304 24-25 0.4539976098084608 2304 26-27 0.5434803228714387 2304 28-29 0.4636275657061759 2304 30-31 0.37614007101606717 2304 32-33 0.34064265528684956 2304 34-35 0.39095694987609164 2304 36-37 0.3715225671344271 2304 38-39 0.3206109822602343 2304 40-41 0.41629225069287656 2304 42-43 0.3803436455297273 2304 44-45 0.46957830490860886 2304 46-47 0.4255358932727802 2304 48-49 0.4204125486128092 2304 50-51 0.2996724756331801 2304 52-53 0.29328310373011135 2304 54-55 0.3721516700333609 2304 56-57 0.4455506159406255 2304 58-59 0.44900773544998174 2304 60-61 0.5319405675233995 2304 62-63 0.48150421684777456 2304 64-65 0.48540416114923346 2304 66-67 0.4377320685181232 2304 68-69 0.45212187510494317 2304 70-71 0.5264468762836145 2304 72-73 0.47996786224395294 2304 74-75 0.5143831801168446 2304 76-77 0.5584391675580846 2304 78-79 0.4478724222603816 2304 80-81 0.529371332646484 2304 82-83 0.6020581189290297 2304 84-85 0.575802367288766 2304 86-87 0.6157407063318772 2304 88-89 0.4406233412550904 2304 90-91 0.5090706048325302 2304 92-93 0.590403233464663 2304 94-95 0.5231141552100169 2304 96-97 0.4732198607773128 2304 98-99 0.4657091433276648 2304 100-101 0.48367814904644035 2202 1 0.21013346296922464 2202 2 0.22608192205217392 2202 3 0.1984410287089986 2202 4 0.27984857936439766 2202 5 0.3316142569585452 2202 6 0.2512244049745931 2202 7 0.26686742379188644 2202 8 0.2943434851173379 2202 9 0.2607467431757726 2202 10-11 0.2173066390139411 2202 12-13 0.24134741566346207 2202 14-15 0.3379153638861183 2202 16-17 0.3349809627830993 2202 18-19 0.31538541534831666 2202 20-21 0.39801877935069996 2202 22-23 0.30418845440853914 2202 24-25 0.35472858172209953 2202 26-27 0.35760012749722847 2202 28-29 0.3442877772582307 2202 30-31 0.3461196240839044 2202 32-33 0.2832804789850556 2202 34-35 0.2268414537391621 2202 36-37 0.19139768532232182 2202 38-39 0.24021241288870243 2202 40-41 0.31180266081635466 2202 42-43 0.2328629227334389 2202 44-45 0.3345523002885429 2202 46-47 0.3529538487424446 2202 48-49 0.36878654068255656 2202 50-51 0.3141244345375469 2202 52-53 0.30657620392165086 2202 54-55 0.2804290980966968 2202 56-57 0.34847382710990615 2202 58-59 0.4825961825523777 2202 60-61 0.4091896512470612 2202 62-63 0.4205505604250632 2202 64-65 0.41362043494213907 2202 66-67 0.41838068130293493 2202 68-69 0.48430559721371935 2202 70-71 0.5409490133203576 2202 72-73 0.46880270236617605 2202 74-75 0.3007423927562769 2202 76-77 0.4059060519610611 2202 78-79 0.39608619240092224 2202 80-81 0.4772863327292356 2202 82-83 0.4593684882794946 2202 84-85 0.507641252661692 2202 86-87 0.46846657426250715 2202 88-89 0.4282022831480319 2202 90-91 0.34855690029135644 2202 92-93 0.40732493508657797 2202 94-95 0.4941375644909769 2202 96-97 0.4723584585586025 2202 98-99 0.5084382531334413 2202 100-101 0.5587861108448564 1102 1 0.07312195720849246 1102 2 0.026019765227538016 1102 3 0.0844965021413131 1102 4 0.1219355223577736 1102 5 0.09983132292084917 1102 6 0.047705035504414184 1102 7 0.1000905164877608 1102 8 0.1234528291064052 1102 9 0.1345711497780897 1102 10-11 0.2064460344658201 1102 12-13 0.12433292966334619 1102 14-15 0.1342270753312178 1102 16-17 0.16412355686983204 1102 18-19 0.19925303848901166 1102 20-21 0.10668258920823348 1102 22-23 0.13657949715123152 1102 24-25 0.16168216483115572 1102 26-27 0.22228566500529467 1102 28-29 0.15360414175390247 1102 30-31 0.15841904992466027 1102 32-33 0.24397840344280297 1102 34-35 0.24268325419145498 1102 36-37 0.21018549350532822 1102 38-39 0.10334047165230231 1102 40-41 0.1361702770861868 1102 42-43 0.1385437141888204 1102 44-45 0.17110454367631434 1102 46-47 0.19060018598039363 1102 48-49 0.30551798491956816 1102 50-51 0.2137096303671271 1102 52-53 0.29448697145659963 1102 54-55 0.3419826359070939 1102 56-57 0.41811459231858095 1102 58-59 0.4384869219952847 1102 60-61 0.4817559036003374 1102 62-63 0.3257257295465976 1102 64-65 0.35792719617159463 1102 66-67 0.3486764525775996 1102 68-69 0.366012792191313 1102 70-71 0.45704972283572687 1102 72-73 0.4133300368911108 1102 74-75 0.37679650060339753 1102 76-77 0.4035291607544025 1102 78-79 0.3989156100751572 1102 80-81 0.42585530251115244 1102 82-83 0.3497045743773697 1102 84-85 0.4400229154414106 1102 86-87 0.26985392538550457 1102 88-89 0.37822692625546495 1102 90-91 0.3812078213804071 1102 92-93 0.5566403572916556 1102 94-95 0.54280282459262 1102 96-97 0.22056528447237866 1102 98-99 0.18443347395335508 1102 100-101 0.44587675429191265 2305 1 0.1704263903340575 2305 2 0.14715718823843105 2305 3 0.15063964585489487 2305 4 0.19895318375922244 2305 5 0.2441675295436312 2305 6 0.22133745896520907 2305 7 0.24891617355258688 2305 8 0.186078755136883 2305 9 0.09808231815993906 2305 10-11 0.16843034972814053 2305 12-13 0.12877322815435122 2305 14-15 0.16858506098225945 2305 16-17 0.16871555653109027 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-0.3994500287250702 1213 60-61 -0.4407614568238287 1213 62-63 -0.339837225759247 1213 64-65 -0.4031667963192902 1213 66-67 -0.33023263793181457 1213 68-69 -0.3805398529240307 1213 70-71 -0.3703094730782297 1213 72-73 -0.45015054680163047 1213 74-75 -0.39134614558907543 1213 76-77 -0.27404767244923534 1213 78-79 -0.19330449911031877 1213 80-81 -0.2214959001337391 1213 82-83 -0.2796557277896383 1213 84-85 -0.3599232694418859 1213 86-87 -0.3205562950077976 1213 88-89 -0.35365598183265234 1213 90-91 -0.40600044765505316 1213 92-93 -0.3704244972742643 1213 94-95 -0.2814884150702568 1213 96-97 -0.23899932554181902 1213 98-99 -0.324241517363685 1213 100-101 -0.11072762587664542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 654.0 3 313.0 4 127.0 5 199.0 6 404.0 7 1488.0 8 5169.0 9 9538.0 10 9153.0 11 9870.0 12 7670.0 13 6723.0 14 6682.0 15 7047.0 16 7551.0 17 8145.0 18 9170.0 19 10282.0 20 11960.0 21 14002.0 22 16302.0 23 17999.0 24 20492.0 25 23772.0 26 29430.0 27 35764.0 28 43941.0 29 52934.0 30 64852.0 31 84429.0 32 116475.0 33 167911.0 34 256185.0 35 434156.0 36 871470.0 37 1656944.0 38 604894.0 39 597.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.09634855183632 32.78618642971367 16.09557639725197 18.021888621198038 2 30.682880406873263 25.596119519022132 29.965864213500993 13.755135860603609 3 31.89617692900073 23.79170398900422 25.29413612372639 19.01798295826866 4 28.03102515969692 23.32574619988144 29.570630624425633 19.072598015996007 5 29.218270034525833 21.779543697524318 30.59698566654746 18.40520060140239 6 27.390657789779915 21.999503728101892 32.04041368116825 18.569424800949932 7 31.525205620439962 22.72453604828159 25.34859297403871 20.401665357239736 8 27.521216019244605 27.13176045820237 25.13551386593853 20.2115096566145 9 25.98731033909517 24.762263670650654 28.52112980927412 20.729296180980057 10-11 22.782667507121776 29.035675155082906 30.884182220045936 17.297475117749382 12-13 22.82498029444995 28.489062201994823 29.982343480780564 18.70361402277466 14-15 22.20825101330351 28.500945086077355 30.723842958223873 18.566960942395262 16-17 22.344319450303807 28.37087522763131 30.980585779528862 18.30421954253602 18-19 21.87273748494362 28.36637890451253 31.081894793117513 18.67898881742633 20-21 21.99868851664176 29.015643968307515 30.444022290671597 18.54164522437913 22-23 22.16708681921436 28.972118867388772 30.255950381600087 18.604843931796776 24-25 21.559059450267622 29.404685292153342 30.30186081170159 18.73439444587744 26-27 21.410631462807864 29.371917039903327 30.463943686536453 18.753507810752357 28-29 21.180581519860056 29.63772017204166 30.353611733202317 18.828086574895963 30-31 21.162969833686923 29.582642510279488 30.523187177053305 18.731200478980277 32-33 20.632016033769812 29.627241287368967 31.028310992642595 18.71243168621863 34-35 20.602959489305974 30.26081173932347 30.646928648490064 18.489300122880493 36-37 20.712035717501323 29.904642020504387 30.85373621667046 18.529586045323835 38-39 20.850234473352405 29.206253446185652 31.00459216935606 18.93891991110588 40-41 20.989401077335753 29.65057987134291 30.106277423382465 19.253741627938876 42-43 21.37861959243277 29.234395693776484 30.356355296815735 19.03062941697501 44-45 20.763225525950077 29.489000608991688 30.56927017746783 19.178503687590403 46-47 21.099510197004832 29.678669729741447 30.156346849873216 19.065473223380508 48-49 20.80928876399564 29.835679415854976 30.159184719393796 19.19584710075559 50-51 20.891405528251187 29.335588282269388 30.081138720317924 19.6918674691615 52-53 21.329480251020975 29.257415245858816 29.768224574082687 19.644879929037526 54-55 21.42343133808634 29.391286544608025 29.739956918567213 19.44532519873843 56-57 21.44201042640895 29.186556582080968 29.88881869963152 19.482614291878566 58-59 21.384043236591207 29.441570695488174 29.873167465960066 19.301218601960553 60-61 20.79756290295888 29.639751186836023 29.602081761066234 19.960604149138867 62-63 20.97938539550853 29.692271071934307 29.476504098532864 19.8518394340243 64-65 20.706570833016105 29.676663772538646 29.818796251402073 19.79796914304317 66-67 20.923040329981735 29.207406305825145 29.79204682236501 20.07750654182811 68-69 20.743237857255494 29.258646653023103 30.52773435943113 19.470381130290278 70-71 20.688459043450102 30.262437715309282 29.7990320355303 19.25007120571032 72-73 20.637672859471024 30.47525145361808 29.501537443174385 19.385538243736512 74-75 20.79546194119336 30.102790285012627 29.449377130138902 19.65237064365511 76-77 20.926946974201353 29.86971255535592 29.506997941176788 19.69634252926594 78-79 20.6334243774381 29.751625039800206 29.927417415921383 19.687533166840307 80-81 20.628847428938926 30.008716452542444 29.67631505470419 19.68612106381444 82-83 20.30847524206303 30.07595151730243 29.586349201912693 20.029224038721853 84-85 20.380362748045293 30.061451046346928 29.735362382426462 19.822823823181317 86-87 20.459124630221346 29.91753879141396 30.04260214102734 19.580734437337348 88-89 20.649031247325272 30.27589651470058 29.648718134846387 19.426354103127768 90-91 20.93651402494091 29.946550247024884 29.325221105930993 19.791714622103214 92-93 20.573877053452026 30.234258474273396 29.3129586300977 19.878905842176877 94-95 20.146656655189858 30.55447704383747 29.33556566306052 19.963300637912155 96-97 20.382541662591418 30.69103346492193 29.142958461263678 19.78346641122297 98-99 20.606157181985164 30.320511792787013 29.005324964067654 20.068006061160172 100-101 20.615954723754708 30.170407798663806 28.955999798051895 20.25763767952959 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 229.0 1 269.0 2 309.0 3 290.0 4 258.0 5 275.5 6 337.5 7 443.0 8 597.0 9 768.5 10 946.5 11 1106.5 12 1180.0 13 1091.5 14 969.0 15 878.0 16 783.0 17 758.5 18 862.5 19 1068.0 20 1438.0 21 1985.5 22 2919.5 23 4521.5 24 6727.5 25 9627.5 26 14075.0 27 20085.5 28 26827.0 29 34060.5 30 41806.0 31 50893.5 32 65309.5 33 88024.5 34 116881.5 35 154102.5 36 200769.5 37 256944.0 38 310423.0 39 327030.0 40 331173.0 41 317523.5 42 283084.0 43 268772.0 44 252114.5 45 237108.5 46 239236.5 47 221667.0 48 175274.5 49 130969.5 50 99462.5 51 80027.5 52 63904.0 53 48096.0 54 37410.5 55 28696.0 56 19769.0 57 12945.0 58 8566.0 59 5964.0 60 4343.5 61 3239.0 62 2244.5 63 1487.0 64 1006.0 65 705.0 66 500.0 67 362.5 68 264.5 69 202.5 70 172.0 71 128.5 72 102.0 73 71.0 74 56.0 75 54.0 76 46.0 77 34.0 78 21.5 79 23.5 80 23.5 81 15.5 82 10.5 83 8.0 84 9.0 85 7.0 86 6.5 87 6.0 88 5.5 89 5.5 90 3.5 91 2.5 92 2.0 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22343099889419712 2 0.2074948093863075 3 0.18117955479865264 4 0.1657839415970008 5 0.17624949888576413 6 0.13531706097743978 7 0.12162966890349936 8 0.11730505845359714 9 0.10530426445511853 10-11 0.09041679298133022 12-13 0.11756453508059128 14-15 0.11402916603779623 16-17 0.10450421152188663 18-19 0.09857949520552063 20-21 0.10237434087530982 22-23 0.08973566683547063 24-25 0.41001631675522743 26-27 0.19680221004892431 28-29 0.09301155925127154 30-31 0.17774148949098037 32-33 0.20940844951038923 34-35 0.2084678467375355 36-37 0.2146196051025214 38-39 0.2160251034987396 40-41 0.21171130457496215 42-43 0.22779885544859835 44-45 0.2094949417193873 46-47 0.22907461553131947 48-49 0.21810091651469263 50-51 0.22165790860973722 52-53 0.21321410670630314 54-55 0.19496425060771588 56-57 0.20313776435803105 58-59 0.1980455355532712 60-61 0.17769824338648135 62-63 0.1659785490672464 64-65 0.17027072493877432 66-67 0.1824661264074985 68-69 0.17536295374353417 70-71 0.16842195397144114 72-73 0.1716437887566183 74-75 0.16168637319571846 76-77 0.17253033389884823 78-79 0.17047614393514468 80-81 0.17594677615427096 82-83 0.2014295432303197 84-85 0.19961320684136075 86-87 0.20397025186963724 88-89 0.21058690585798756 90-91 0.1916559236135407 92-93 0.17203300369710947 94-95 0.17282224510421662 96-97 0.18190392704901123 98-99 0.1962508222165618 100-101 0.20859758505103257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4624694.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.51283765464774 #Duplication Level Percentage of deduplicated Percentage of total 1 91.09243485960053 46.92429808489177 2 4.248538883854335 4.377085875868933 3 1.1921902966240894 1.8423931561032916 4 0.736740654704028 1.5180640695738994 5 0.45011741257353105 1.1593412599715198 6 0.2478756444155876 0.7661266697592811 7 0.23456158285446327 0.8458052916319418 8 0.19996033950926342 0.8240419605207143 9 0.14379298268461424 0.6666466115619096 >10 1.1748703765764514 12.383089499527214 >50 0.14586224089329378 5.1286967178298895 >100 0.10713436473714393 10.267118261340928 >500 0.016374243333689743 5.920550110603449 >1k 0.009504219817477467 7.172871710731121 >5k 4.1897821424711754E-5 0.20387072008429416 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 9446 0.20425135154888086 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.730373512279948E-4 0.0 0.0 2 0.0010595295602260388 0.0 0.0 3 0.0015784828142143025 0.0 0.0 4 0.0025298971131927864 0.0 0.0 5 0.002962358158183006 0.0 0.0 6 0.005600370532623348 0.0 0.0 7 0.007243722503586183 0.0 0.0 8 0.008757336161051952 0.0 0.0 9 0.009708750460030436 0.0 0.0 10-11 0.01361171139106717 0.0 0.0 12-13 0.019590485338056962 0.0 0.0 14-15 0.02804509876761576 0.0 0.0 16-17 0.03934314356798525 0.0 1.0811526124755498E-5 18-19 0.052273728813192824 0.0 3.243457837426649E-5 20-21 0.06759366133197137 0.0 4.324610449902199E-5 22-23 0.08570296759093682 0.0 4.324610449902199E-5 24-25 0.10552049497761365 0.0 4.324610449902199E-5 26-27 0.12517584947241914 0.0 4.324610449902199E-5 28-29 0.14680971324805492 0.0 4.324610449902199E-5 30-31 0.17031397104327334 0.0 5.405763062377748E-5 32-33 0.19306142200975893 0.0 6.486915674853298E-5 34-35 0.21764683241745292 0.0 6.486915674853298E-5 36-37 0.24584329255081527 0.0 6.486915674853298E-5 38-39 0.27740213730897656 0.0 6.486915674853298E-5 40-41 0.31236661279643585 0.0 6.486915674853298E-5 42-43 0.35023938881145433 0.0 6.486915674853298E-5 44-45 0.3932260166834822 0.0 6.486915674853298E-5 46-47 0.4370883781716153 0.0 6.486915674853298E-5 48-49 0.4848428890646603 0.0 6.486915674853298E-5 50-51 0.5379923514939583 0.0 6.486915674853298E-5 52-53 0.5936825225625739 0.0 6.486915674853298E-5 54-55 0.652572905364117 0.0 6.486915674853298E-5 56-57 0.7133661167636172 0.0 6.486915674853298E-5 58-59 0.7779109277284075 0.0 6.486915674853298E-5 60-61 0.8453964737991313 0.0 6.486915674853298E-5 62-63 0.9172606879503811 0.0 8.649220899804398E-5 64-65 0.9915466839535761 0.0 9.730373512279948E-5 66-67 1.0695842795220614 0.0 1.0811526124755498E-4 68-69 1.1524330042160627 0.0 1.0811526124755498E-4 70-71 1.2374656571872649 0.0 1.0811526124755498E-4 72-73 1.327049962656989 0.0 1.0811526124755498E-4 74-75 1.4175532478473172 0.0 1.0811526124755498E-4 76-77 1.5158624548997186 0.0 1.2973831349706596E-4 78-79 1.619361194492003 0.0 1.4054983962182146E-4 80-81 1.7295306457032615 0.0 1.5136136574657696E-4 82-83 1.8429111201735724 0.0 1.5136136574657696E-4 84-85 1.9627676987926121 0.0 1.5136136574657696E-4 86-87 2.0893598581873745 0.0 1.5136136574657696E-4 88-89 2.2216605033760075 0.0 1.7298441799608796E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGCG 25 5.281903E-5 75.98582 1 ATCGGGA 790 0.0 55.89759 2 CGACGGG 125 0.0 49.390774 1 CGAGCGC 50 2.8314535E-5 47.491135 1 GTCGACC 40 5.3948426E-4 47.49113 1 CGAGTCG 40 5.3948426E-4 47.49113 1 CGAGGCG 180 0.0 47.49113 1 CGGGAGA 800 0.0 44.50799 4 AGAGCGG 620 0.0 43.619144 8 GAGCGGC 640 0.0 40.773376 9 GAGAGCG 780 0.0 40.75569 7 CGACGCC 85 3.6492202E-7 39.110344 1 GTCGCAC 50 0.0016178039 37.99291 1 CGCGGCG 50 0.0016178039 37.99291 1 GCGCGCT 50 0.0016203026 37.980976 3 GATCCGG 240 0.0 37.597145 1 CGGACGC 90 5.711281E-7 36.937546 1 CGCCGAG 65 1.3302662E-4 36.53164 1 CGGACCC 110 7.032031E-8 34.539 1 CGAGGGG 485 0.0 34.27195 1 >>END_MODULE