FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624694
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT94460.20425135154888086Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCG255.281903E-575.985821
ATCGGGA7900.055.897592
CGACGGG1250.049.3907741
CGAGCGC502.8314535E-547.4911351
GTCGACC405.3948426E-447.491131
CGAGTCG405.3948426E-447.491131
CGAGGCG1800.047.491131
CGGGAGA8000.044.507994
AGAGCGG6200.043.6191448
GAGCGGC6400.040.7733769
GAGAGCG7800.040.755697
CGACGCC853.6492202E-739.1103441
GTCGCAC500.001617803937.992911
CGCGGCG500.001617803937.992911
GCGCGCT500.001620302637.9809763
GATCCGG2400.037.5971451
CGGACGC905.711281E-736.9375461
CGCCGAG651.3302662E-436.531641
CGGACCC1107.032031E-834.5391
CGAGGGG4850.034.271951
Produced by FastQC (version 0.11.2)