FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220003
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC48250.077.785389
ATCGGGA86250.075.823182
CGGGAGA76350.073.3844764
TCGGGAG79350.072.707183
AGAGCGG53950.071.765798
GATCGGG94950.070.2430651
ATTCGGA11150.068.576942
ATCCGGA12600.068.223692
AAGCGGC14250.064.263039
TCGGAGA15800.064.010884
CGGAAAG27900.063.1388665
TCGGGAA20550.061.694633
GAGAGCG72250.060.682197
GATCCGG15200.059.6926881
GTTCGGA10800.058.4864162
GAGCGTC126800.058.2623679
AGAGCGT127850.058.192118
CGGAGAG31300.058.1007235
ATCGGAG13850.057.5980033
AATCGGA10800.057.167172