##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3534150 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01117439837019 33.0 33.0 33.0 33.0 33.0 2 31.903126918778206 33.0 33.0 33.0 33.0 33.0 3 31.865428745242845 33.0 33.0 33.0 33.0 33.0 4 35.562244103957106 37.0 37.0 37.0 33.0 37.0 5 35.535591019056916 37.0 37.0 37.0 33.0 37.0 6 35.68592759220746 37.0 37.0 37.0 33.0 37.0 7 35.50813038495819 37.0 37.0 37.0 33.0 37.0 8 35.53536380742187 37.0 37.0 37.0 33.0 37.0 9 35.40318237765799 37.0 37.0 37.0 33.0 37.0 10-11 35.35927323401667 37.0 37.0 37.0 33.0 37.0 12-13 35.36097123777995 37.0 37.0 37.0 33.0 37.0 14-15 37.10862286546977 40.0 37.0 40.0 33.0 40.0 16-17 37.062438351513094 40.0 37.0 40.0 33.0 40.0 18-19 37.01360921296493 40.0 37.0 40.0 33.0 40.0 20-21 36.96044154888729 40.0 37.0 40.0 33.0 40.0 22-23 37.14552636418941 40.0 37.0 40.0 33.0 40.0 24-25 36.84461115119619 40.0 37.0 40.0 33.0 40.0 26-27 36.749349348499635 40.0 37.0 40.0 33.0 40.0 28-29 36.629735721460605 40.0 37.0 40.0 33.0 40.0 30-31 36.57936802342854 40.0 37.0 40.0 33.0 40.0 32-33 36.47301798169291 40.0 37.0 40.0 33.0 40.0 34-35 36.504975878216825 40.0 37.0 40.0 33.0 40.0 36-37 36.34304245716792 40.0 37.0 40.0 33.0 40.0 38-39 36.27924055289108 40.0 37.0 40.0 33.0 40.0 40-41 36.322419110677245 40.0 37.0 40.0 33.0 40.0 42-43 36.14682229107423 38.5 37.0 40.0 30.0 40.0 44-45 35.93552947667756 37.0 37.0 40.0 27.0 40.0 46-47 35.81340576942122 37.0 37.0 40.0 27.0 40.0 48-49 35.68947002815387 37.0 37.0 40.0 27.0 40.0 50-51 34.61171710312239 37.0 35.0 38.5 27.0 38.5 52-53 35.01705770835986 37.0 35.0 38.5 27.0 40.0 54-55 35.73303566628468 37.0 37.0 40.0 27.0 40.0 56-57 35.8162740687294 37.0 37.0 40.0 27.0 40.0 58-59 35.58390376752543 37.0 37.0 40.0 27.0 40.0 60-61 35.4217589802357 37.0 37.0 40.0 27.0 40.0 62-63 35.17186310711203 37.0 35.0 40.0 27.0 40.0 64-65 34.96874085706605 37.0 33.0 40.0 27.0 40.0 66-67 34.75765615494532 37.0 33.0 40.0 27.0 40.0 68-69 34.60564492169263 37.0 33.0 40.0 27.0 40.0 70-71 34.269429565807904 37.0 33.0 38.5 27.0 40.0 72-73 34.14882899707143 37.0 33.0 37.0 27.0 40.0 74-75 33.93910657442384 37.0 33.0 37.0 27.0 40.0 76-77 33.633134699998585 37.0 33.0 37.0 27.0 40.0 78-79 33.24226433513009 37.0 33.0 37.0 22.0 37.0 80-81 32.86355403703861 37.0 33.0 37.0 22.0 37.0 82-83 32.541770015420965 37.0 33.0 37.0 22.0 37.0 84-85 32.29805511933563 37.0 33.0 37.0 22.0 37.0 86-87 32.09130455696561 37.0 33.0 37.0 22.0 37.0 88-89 31.85073652787799 33.0 33.0 37.0 18.5 37.0 90-91 31.662946960372366 33.0 33.0 37.0 15.0 37.0 92-93 31.452886691283616 33.0 33.0 37.0 15.0 37.0 94-95 31.28009365759801 33.0 33.0 37.0 15.0 37.0 96-97 31.115834783469857 33.0 33.0 37.0 15.0 37.0 98-99 30.937094350834005 33.0 33.0 37.0 15.0 37.0 100-101 29.54566076708685 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.12592946562094198 2306 2 0.13461425832446494 2306 3 0.0927576347592165 2306 4 0.22238138673933605 2306 5 0.2821181270371298 2306 6 0.2794123960464745 2306 7 0.29914037690843287 2306 8 0.21834175520560706 2306 9 0.22334986573169857 2306 10-11 0.22312963349241954 2306 12-13 0.18156235440314106 2306 14-15 0.382350265326032 2306 16-17 0.3279814323566015 2306 18-19 0.3964118275165376 2306 20-21 0.32171561249058556 2306 22-23 0.22977562979777844 2306 24-25 0.2683709720926615 2306 26-27 0.26253851304337417 2306 28-29 0.1717493690125309 2306 30-31 0.3132348717235729 2306 32-33 0.2674705941976043 2306 34-35 0.2711928902966605 2306 36-37 0.37582484937838956 2306 38-39 0.22759162766729446 2306 40-41 0.21691900739526915 2306 42-43 0.2350042958871228 2306 44-45 0.4586460435863131 2306 46-47 0.3504666100624476 2306 48-49 0.30820592697764226 2306 50-51 0.3069836514927431 2306 52-53 0.22196772611031435 2306 54-55 0.22019825012073824 2306 56-57 0.22592326089143455 2306 58-59 0.5097985248869321 2306 60-61 0.46789203319153216 2306 62-63 0.434365744090222 2306 64-65 0.3466535536999231 2306 66-67 0.33616418752598776 2306 68-69 0.1846158081859599 2306 70-71 0.21707591481624178 2306 72-73 0.0786976854039736 2306 74-75 0.07370330050524387 2306 76-77 0.20190957825800382 2306 78-79 0.14555320146750717 2306 80-81 0.3484624602463242 2306 82-83 0.43829543316146413 2306 84-85 0.3759582558604748 2306 86-87 0.45673085981297845 2306 88-89 0.3061871539295673 2306 90-91 0.32387497267030696 2306 92-93 0.20773005753000362 2306 94-95 0.11508101992886921 2306 96-97 0.05420873912049373 2306 98-99 0.12503188997415648 2306 100-101 -0.024711149340227223 2307 1 0.20760179234081022 2307 2 0.18910729479530985 2307 3 0.20083120662288323 2307 4 0.2216162439749354 2307 5 0.2896697313073986 2307 6 0.1734804429919805 2307 7 0.2969260975483081 2307 8 0.305760546853854 2307 9 0.25964449000534984 2307 10-11 0.3094166691524478 2307 12-13 0.25018413314700894 2307 14-15 0.47841447416467275 2307 16-17 0.4605099482275037 2307 18-19 0.5235508149272476 2307 20-21 0.4291002672612976 2307 22-23 0.3233781229374699 2307 24-25 0.4003727838529372 2307 26-27 0.29555670670207945 2307 28-29 0.4486970113134632 2307 30-31 0.32841235360766774 2307 32-33 0.41700066038661987 2307 34-35 0.32450222188070654 2307 36-37 0.3199538938378197 2307 38-39 0.1901206833992859 2307 40-41 0.16443568027543165 2307 42-43 0.328121423337123 2307 44-45 0.3753476823873143 2307 46-47 0.2649449718182879 2307 48-49 0.1547888608477166 2307 50-51 0.01811178463946561 2307 52-53 0.157149930930224 2307 54-55 0.304680061555068 2307 56-57 0.1811222591265249 2307 58-59 0.24729377988132484 2307 60-61 0.26700649121512043 2307 62-63 0.410951985033968 2307 64-65 0.3092941467729986 2307 66-67 0.32305710692507006 2307 68-69 0.20079704125588904 2307 70-71 0.182307331880331 2307 72-73 0.3308312402501272 2307 74-75 0.19010915314285626 2307 76-77 0.2214110419946138 2307 78-79 0.35086933937587617 2307 80-81 0.262683369372418 2307 82-83 0.31999488875059257 2307 84-85 0.4512547041176802 2307 86-87 0.26916791845954435 2307 88-89 0.3047543150275125 2307 90-91 0.3448548579209678 2307 92-93 0.4776745299295193 2307 94-95 0.39732057429560186 2307 96-97 0.44002132515024783 2307 98-99 0.22215383610891948 2307 100-101 0.023423834270403177 2201 1 0.22582374551979 2201 2 0.15434948993828712 2201 3 0.16483553213855018 2201 4 0.2979302974029281 2201 5 0.23590751972196244 2201 6 0.25607692685500894 2201 7 0.3852627606041281 2201 8 0.27228777748679533 2201 9 0.24534042699425385 2201 10-11 0.22931825804854356 2201 12-13 0.2194960238171646 2201 14-15 0.44932063346409734 2201 16-17 0.3005522350784062 2201 18-19 0.36124745909174294 2201 20-21 0.351102923542733 2201 22-23 0.3334973869750968 2201 24-25 0.29219334866883173 2201 26-27 0.3410256915552736 2201 28-29 0.34518933920804074 2201 30-31 0.30934375757609445 2201 32-33 0.34262524885665613 2201 34-35 0.2360282780524301 2201 36-37 0.3075322485176102 2201 38-39 0.2710829169746347 2201 40-41 0.23616464961074968 2201 42-43 0.25299573099849226 2201 44-45 0.3230085679560659 2201 46-47 0.3276312145044571 2201 48-49 0.22271722995838417 2201 50-51 0.1587785037820595 2201 52-53 0.2940880480689856 2201 54-55 0.3980437934430654 2201 56-57 0.4537581249230982 2201 58-59 0.41923521424831023 2201 60-61 0.4312842537915458 2201 62-63 0.41323172045534307 2201 64-65 0.4114397874603384 2201 66-67 0.38837714135927115 2201 68-69 0.4430390377400002 2201 70-71 0.5183950266503601 2201 72-73 0.3969698054333506 2201 74-75 0.2494961281180892 2201 76-77 0.3524829440095658 2201 78-79 0.3086662000660354 2201 80-81 0.4459041605844547 2201 82-83 0.3560770860425819 2201 84-85 0.28724041442307424 2201 86-87 0.33457025888587566 2201 88-89 0.3086553379332564 2201 90-91 0.35614374164555684 2201 92-93 0.48133128989088547 2201 94-95 0.38589612776045 2201 96-97 0.3226139357901161 2201 98-99 0.3392886942427502 2201 100-101 0.29982469162708725 1101 1 0.2154136719384141 1101 2 0.279112356521086 1101 3 0.18232180908948337 1101 4 0.3163725082394535 1101 5 0.4022997979585057 1101 6 0.33759220104754917 1101 7 0.5448841306889705 1101 8 0.35880864537261203 1101 9 0.4079673008089486 1101 10-11 0.3387500519916884 1101 12-13 0.3343545708498965 1101 14-15 0.5635731607832284 1101 16-17 0.5628075019287806 1101 18-19 0.5384001603885196 1101 20-21 0.5363456579083206 1101 22-23 0.4198349906688179 1101 24-25 0.5537358005202648 1101 26-27 0.5524666443549506 1101 28-29 0.5336866453840301 1101 30-31 0.6040908370297515 1101 32-33 0.5938477356487226 1101 34-35 0.4239820310506133 1101 36-37 0.5005913530333359 1101 38-39 0.4949616027541808 1101 40-41 0.4291766684334206 1101 42-43 0.4599635429575244 1101 44-45 0.5463268686921623 1101 46-47 0.5095352902826775 1101 48-49 0.4737766217766506 1101 50-51 0.5841579099944667 1101 52-53 0.511678508464037 1101 54-55 0.6335949427257503 1101 56-57 0.5892558765631151 1101 58-59 0.5801508967586955 1101 60-61 0.6007568359883635 1101 62-63 0.6324639407090089 1101 64-65 0.5905190844107153 1101 66-67 0.4943019580588057 1101 68-69 0.4515449339715403 1101 70-71 0.5116257871749852 1101 72-73 0.4753954165679204 1101 74-75 0.36206590418172624 1101 76-77 0.32530637338610546 1101 78-79 0.5745826153429903 1101 80-81 0.5311155528554323 1101 82-83 0.4413229401074048 1101 84-85 0.5575973950765842 1101 86-87 0.5799564413752165 1101 88-89 0.5936239740732994 1101 90-91 0.6230127534554306 1101 92-93 0.5054376411632155 1101 94-95 0.5383107024148508 1101 96-97 0.4867198372453494 1101 98-99 0.46148397580649103 1101 100-101 0.32837530838437345 2304 1 0.3353490966951682 2304 2 0.31405478221790517 2304 3 0.35686073206856506 2304 4 0.3841892065723158 2304 5 0.43197056205922024 2304 6 0.467257147558783 2304 7 0.522971254380181 2304 8 0.5548574443709313 2304 9 0.5313323267831649 2304 10-11 0.4371228457700411 2304 12-13 0.42538715331753707 2304 14-15 0.5454313385291059 2304 16-17 0.5042073858414184 2304 18-19 0.500899251390166 2304 20-21 0.527130860605034 2304 22-23 0.4668753581289238 2304 24-25 0.5937124243879808 2304 26-27 0.5695652009316703 2304 28-29 0.5253327852150775 2304 30-31 0.4609938036037349 2304 32-33 0.5784036882988346 2304 34-35 0.4732071677970211 2304 36-37 0.5998139115298642 2304 38-39 0.4224684880035454 2304 40-41 0.5264408808706307 2304 42-43 0.5850643260844066 2304 44-45 0.43866589665229583 2304 46-47 0.5683455667900503 2304 48-49 0.5586998097627927 2304 50-51 0.45402253204287035 2304 52-53 0.44821647418521593 2304 54-55 0.5587379033763682 2304 56-57 0.6141517879876659 2304 58-59 0.6256328113273923 2304 60-61 0.6010146609767659 2304 62-63 0.6868509320500564 2304 64-65 0.6600462862691785 2304 66-67 0.46649414477169415 2304 68-69 0.582613572272173 2304 70-71 0.6357843191327319 2304 72-73 0.38769723839894965 2304 74-75 0.4880047507388383 2304 76-77 0.5786908221761138 2304 78-79 0.5710797653852282 2304 80-81 0.6289523350938069 2304 82-83 0.59334263529216 2304 84-85 0.7335180616665653 2304 86-87 0.6544623759775874 2304 88-89 0.5562946839646621 2304 90-91 0.4956937499027312 2304 92-93 0.5951074532947338 2304 94-95 0.5235625180977515 2304 96-97 0.445067086517156 2304 98-99 0.5130266947627078 2304 100-101 0.6495808186993557 2202 1 0.1895261269152897 2202 2 0.2210470717341373 2202 3 0.21510027524313458 2202 4 0.2089389267563746 2202 5 0.32129040204595327 2202 6 0.275676498385792 2202 7 0.3272503656018344 2202 8 0.2687793239049867 2202 9 0.28252672631887776 2202 10-11 0.25191060019528067 2202 12-13 0.2564418003349047 2202 14-15 0.3956145143364225 2202 16-17 0.3655909720454602 2202 18-19 0.39815368699103715 2202 20-21 0.41928383701759486 2202 22-23 0.4399158692593801 2202 24-25 0.4489841049418075 2202 26-27 0.4569043067642795 2202 28-29 0.48795274334632666 2202 30-31 0.48022726337242005 2202 32-33 0.339023739702057 2202 34-35 0.3528824258495007 2202 36-37 0.33759139567362695 2202 38-39 0.34106277561288323 2202 40-41 0.45827201713063204 2202 42-43 0.5161959224861761 2202 44-45 0.4986125761697693 2202 46-47 0.4411148119088253 2202 48-49 0.4602123703895842 2202 50-51 0.44554644076069394 2202 52-53 0.4806518056116573 2202 54-55 0.49476368789736824 2202 56-57 0.5743337201685748 2202 58-59 0.57423994974301 2202 60-61 0.5489805545223376 2202 62-63 0.6326948880256822 2202 64-65 0.6040610750599598 2202 66-67 0.5896209324846637 2202 68-69 0.5422436452315864 2202 70-71 0.6433833947037542 2202 72-73 0.567941455536122 2202 74-75 0.5519180803412951 2202 76-77 0.6965088796316508 2202 78-79 0.5551691199431232 2202 80-81 0.6480122053421198 2202 82-83 0.7107344916118521 2202 84-85 0.6089973760138676 2202 86-87 0.6331212285249421 2202 88-89 0.6018114107399377 2202 90-91 0.5817034709158726 2202 92-93 0.5745707766790638 2202 94-95 0.5724253771948788 2202 96-97 0.5673803352838966 2202 98-99 0.5807006988252219 2202 100-101 0.5028406744386906 1102 1 0.16708414597125198 1102 2 -0.0831547756816704 1102 3 0.0832947980000327 1102 4 0.10634903403147433 1102 5 0.16490449002805718 1102 6 0.15871898823111508 1102 7 0.19212172167830488 1102 8 0.21401070878447825 1102 9 0.1177764452918808 1102 10-11 0.227172592775446 1102 12-13 0.1894037306042904 1102 14-15 0.11112462322764571 1102 16-17 0.03591252349405494 1102 18-19 0.25193266958352467 1102 20-21 0.23258348452174715 1102 22-23 0.27375832085629526 1102 24-25 0.2528103144353153 1102 26-27 0.2283412570557175 1102 28-29 0.1461914807180662 1102 30-31 0.12211368626687857 1102 32-33 0.23339642100486913 1102 34-35 0.1737042592229372 1102 36-37 0.24648960374493356 1102 38-39 0.17522632075497313 1102 40-41 0.24816954552962045 1102 42-43 0.24319233647395322 1102 44-45 0.1758477957658755 1102 46-47 0.2925428869546991 1102 48-49 0.27554014842674235 1102 50-51 0.2971874184473222 1102 52-53 0.4086067640771205 1102 54-55 0.4814404942814363 1102 56-57 0.5801949960850123 1102 58-59 0.576372343087435 1102 60-61 0.5506495451236475 1102 62-63 0.43932822501027147 1102 64-65 0.5348683902016518 1102 66-67 0.44968766966247387 1102 68-69 0.4215882283240049 1102 70-71 0.5376514765014733 1102 72-73 0.5245006750173076 1102 74-75 0.42334228116516215 1102 76-77 0.5370098977933608 1102 78-79 0.5140693706227921 1102 80-81 0.5695094911420782 1102 82-83 0.49084444679119343 1102 84-85 0.445568281010587 1102 86-87 0.4075295005949897 1102 88-89 0.4155767056117803 1102 90-91 0.46085984223783427 1102 92-93 0.34861934766127334 1102 94-95 0.42726472043568364 1102 96-97 0.2730209421585492 1102 98-99 0.39446958509637753 1102 100-101 0.3208682869123365 2305 1 0.12372397985807027 2305 2 0.09957738171491926 2305 3 0.1316465782819911 2305 4 0.06148292330465921 2305 5 0.0951205959002337 2305 6 0.23347959017385023 2305 7 0.17803622447284084 2305 8 0.16779637878278209 2305 9 0.04395031058597709 2305 10-11 0.11861012125363146 2305 12-13 0.05513202886673696 2305 14-15 0.08937846876478517 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-0.2765769368487767 1213 76-77 -0.18937832425834245 1213 78-79 -0.1959889959637593 1213 80-81 -0.3165731010954005 1213 82-83 -0.28329169484937466 1213 84-85 -0.31079473580435035 1213 86-87 -0.1829284340261168 1213 88-89 -0.30963278602379063 1213 90-91 -0.4511484731211155 1213 92-93 -0.3423797041808676 1213 94-95 -0.3604586418162228 1213 96-97 -0.2947220601608329 1213 98-99 -0.36621894867249694 1213 100-101 2.2968010967971964E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 484.0 3 253.0 4 124.0 5 178.0 6 422.0 7 1544.0 8 6318.0 9 17616.0 10 23704.0 11 19426.0 12 12897.0 13 9808.0 14 8376.0 15 7738.0 16 8072.0 17 8564.0 18 9698.0 19 10751.0 20 12026.0 21 14072.0 22 16205.0 23 18414.0 24 20821.0 25 24749.0 26 30030.0 27 37338.0 28 46666.0 29 54945.0 30 62354.0 31 78521.0 32 103712.0 33 143719.0 34 212654.0 35 336515.0 36 583144.0 37 1017544.0 38 571372.0 39 3376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.59448574960236 29.02968214894074 16.118048027159222 18.257784074297675 2 31.95312542085704 26.718634862372838 27.061558042193585 14.266681674576533 3 35.206768243994205 23.49987400390092 23.513820028816117 17.779537723288758 4 30.30634101991619 23.685881139956344 27.03731855532373 18.970459284803738 5 33.393177534332 21.535607488556003 26.883717634387267 18.187497342724733 6 31.511588273943445 21.873463828735904 28.376106007894098 18.238841889426553 7 33.981866970054774 22.762465466561206 23.01751431763738 20.238153245746638 8 29.39732735345217 28.399666974404674 22.700838711194347 19.50216696094881 9 29.989355603292 24.197988044739322 25.079393943513885 20.733262408454788 10-11 26.02477778863405 29.770389848724932 27.794114106970152 16.41071825567087 12-13 26.48886705722865 28.14122723486395 27.325443624387987 18.044462083519413 14-15 24.966683696022738 28.171309184507244 28.177682799892413 18.68432431957761 16-17 25.591830029259395 27.993720418412178 28.220955164863803 18.19349438746463 18-19 25.02436465670404 28.032893490267874 28.58973316841933 18.35300868460876 20-21 24.47877182768438 29.043928907421574 27.988039923757558 18.489259341136492 22-23 25.509093823472607 28.578977864550147 27.44371548858451 18.468212823392744 24-25 23.42440852686652 30.086149062067697 27.79078202621105 18.698660384854733 26-27 23.25626391869716 30.10922053311436 27.766421458043727 18.86809409014475 28-29 23.506907990600727 29.846518363107993 27.673396469069882 18.973177177221405 30-31 24.07416619507799 28.542516748661445 28.34036074868582 19.04295630757474 32-33 22.887290647908547 29.46738653197097 28.50647607995036 19.138846740170127 34-35 24.332284477045075 28.559149327694698 27.96835426438409 19.14021193087614 36-37 22.947345229477282 29.02651750693072 28.658027401905866 19.36810986168613 38-39 22.772481752507833 29.966478092943188 27.938419729323787 19.322620425225193 40-41 24.194504666304105 28.392675572633802 27.935742409352006 19.47707735171009 42-43 23.905784490927733 28.353005284563736 28.188012438838435 19.553197785670097 44-45 22.717685259797815 29.08757596705056 28.551092785703307 19.64364598744832 46-47 23.211616918145925 29.17697284500434 27.781165117596778 19.830245119252954 48-49 21.8679512447493 28.990216652037184 28.71321816045042 20.428613942763096 50-51 21.45166735361979 28.381259124814633 29.344468070716612 20.822605450848965 52-53 23.543986186623773 28.376966572259004 27.960234512089325 20.118812729027898 54-55 23.366727075438202 28.317827155313857 28.517452512255986 19.797993256991955 56-57 23.582630529318735 28.2015740012509 28.305881779225885 19.90991369020448 58-59 23.073813221215694 28.47672299718123 27.912132618989737 20.53733116261334 60-61 21.870339482329022 28.315751715325604 27.89009671129993 21.92381209104544 62-63 22.79817996490471 28.42218420366096 28.21153592987857 20.568099901555765 64-65 21.809887446763494 29.04466715217765 28.888597903516526 20.25684749754233 66-67 21.466248710066633 28.922598612137286 28.18664388002886 21.42450879776722 68-69 21.24071107117727 28.429241870349337 30.031015650963738 20.299031407509656 70-71 21.150797997732333 29.80794289146939 28.7688162527019 20.27244285809637 72-73 21.154438634416334 30.81285848753653 27.825080898398074 20.20762197964906 74-75 21.00285827688032 30.963891564306838 27.946015395386937 20.0872347634259 76-77 21.129591515557085 30.88254571834571 27.777222718468863 20.21064004762834 78-79 21.042247664941392 31.01439942770179 27.78715846371148 20.156194443645333 80-81 21.116041610066553 30.78635293444052 27.800828817016132 20.2967766384768 82-83 21.238157004086176 30.76986649939221 27.64899298652236 20.342983509999254 84-85 21.177398338394962 30.55980508007503 27.8390997711494 20.42369681038061 86-87 21.43926631120476 30.1722343916481 27.777360369305377 20.61113892784176 88-89 21.475071328572188 30.25328111432065 27.714827482322917 20.556820074784245 90-91 21.5627144773125 29.97118899063947 27.749682421202042 20.716414110845985 92-93 21.653108673402723 29.90867521669123 27.68174612554869 20.756469984357363 94-95 21.572623331877423 29.868857356405336 27.822313324601637 20.736205987115603 96-97 21.683486326951293 29.693853080772918 27.738932529360945 20.883728062914848 98-99 21.698715917858546 29.782676890334564 27.721996294338553 20.796610897468337 100-101 21.929992693277274 29.657497017910117 27.584933917682125 20.827576371130476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 108.0 1 159.0 2 228.0 3 218.0 4 181.0 5 176.5 6 209.5 7 285.0 8 374.5 9 475.5 10 610.0 11 732.0 12 794.0 13 815.5 14 822.5 15 828.0 16 811.0 17 860.0 18 1003.0 19 1311.5 20 1922.5 21 2901.5 22 4191.5 23 6063.0 24 8623.0 25 11817.0 26 15937.5 27 21163.0 28 27240.5 29 34079.5 30 41969.0 31 50843.0 32 61124.5 33 72292.0 34 84193.0 35 97066.0 36 110655.0 37 124361.5 38 136853.0 39 147231.5 40 156377.5 41 163959.5 42 167719.5 43 168427.5 44 167481.0 45 165573.5 46 162432.5 47 157810.0 48 150862.5 49 141999.0 50 132544.0 51 121033.0 52 107075.5 53 92191.0 54 77993.0 55 64771.5 56 53098.5 57 43650.0 58 35748.5 59 28980.0 60 23178.5 61 18273.0 62 14088.0 63 10806.0 64 8482.0 65 6512.0 66 4900.0 67 3728.5 68 2880.0 69 2235.0 70 1700.5 71 1337.5 72 1053.0 73 800.5 74 637.5 75 483.5 76 377.5 77 314.5 78 236.0 79 173.5 80 137.0 81 119.5 82 99.0 83 77.5 84 60.0 85 53.0 86 49.0 87 33.5 88 22.5 89 19.0 90 18.5 91 15.5 92 10.5 93 7.5 94 9.5 95 7.0 96 4.5 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21750633108385325 2 0.20149116477795226 3 0.17721375719762883 4 0.16026484444633082 5 0.17260161566430401 6 0.1323373371249098 7 0.11991567986644597 8 0.11575626388240454 9 0.10339119731760112 10-11 0.08955477271762659 12-13 0.11625143245193327 14-15 0.11237496993619399 16-17 0.10356096939858239 18-19 0.09679838150616131 20-21 0.10102853585727826 22-23 0.08692330546241671 24-25 0.4002801239336191 26-27 0.19086626204320697 28-29 0.0916486283830624 30-31 0.17490768643096644 32-33 0.20617404467835262 34-35 0.20634381675933394 36-37 0.21111158270022495 38-39 0.2122433965734335 40-41 0.2087064782196568 42-43 0.2245660201179916 44-45 0.2063296690859188 46-47 0.22489141660653905 48-49 0.21494560219571893 50-51 0.21811468104070286 52-53 0.21005050719409193 54-55 0.19219614334422705 56-57 0.20055741833255522 58-59 0.19530863149555056 60-61 0.17473791434998515 62-63 0.16288216402812555 64-65 0.16718305674631806 66-67 0.17971789539210276 68-69 0.1723894005630774 70-71 0.16554192663016568 72-73 0.16861197176124387 74-75 0.15917547359336756 76-77 0.16972963796103732 78-79 0.16749430556145042 80-81 0.17287042145919104 82-83 0.19842111964687406 84-85 0.19687902324462742 86-87 0.20078378110719694 88-89 0.20761710736669353 90-91 0.18898462147899778 92-93 0.16900810661686685 94-95 0.16997014840909414 96-97 0.179222726822574 98-99 0.19366750137939814 100-101 0.2054525133341822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3534150.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.25093790586354 #Duplication Level Percentage of deduplicated Percentage of total 1 97.8605002875749 93.21304436328536 2 1.5599762886200048 2.9717840920392704 3 0.2875185983870399 0.8215924848523456 4 0.08831612746682603 0.336487758937159 5 0.07335286276112402 0.3493464488038576 6 0.02841278152063104 0.16238064530126964 7 0.01875495524253339 0.12504989540636585 8 0.014786276725274968 0.1126725380974465 9 0.010234199678154546 0.08773354062540965 >10 0.0551683836859837 0.9940995604848379 >50 0.0021671948339660953 0.13375716955898637 >100 7.824312873257034E-4 0.10215302337641498 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.961221624202018E-5 0.5898984792313198 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 20914 0.5917688836070908 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003791576475248645 0.0 0.0 2 0.005206343816759334 0.0 0.0 3 0.007894401765629642 0.0 0.0 4 0.010412687633518668 0.0 2.8295346830213772E-5 5 0.013440289744351542 0.0 2.8295346830213772E-5 6 0.023202184400775292 0.0 5.6590693660427545E-5 7 0.034095892930407594 0.0 8.488604049064132E-5 8 0.042527906285811296 0.0 8.488604049064132E-5 9 0.04824356634551448 0.0 1.1318138732085509E-4 10-11 0.057948870308277806 0.0 1.4147673415106885E-4 12-13 0.07020075548576037 0.0 1.4147673415106885E-4 14-15 0.08103787332173223 0.0 1.4147673415106885E-4 16-17 0.0945206060863291 0.0 1.4147673415106885E-4 18-19 0.11163929091860844 0.0 1.4147673415106885E-4 20-21 0.13297398242858963 0.0 1.697720809812826E-4 22-23 0.15524242038396785 0.0 1.980674278114964E-4 24-25 0.17676103164834545 0.0 1.980674278114964E-4 26-27 0.19854844870761004 0.0 2.2636277464171018E-4 28-29 0.22217506331083853 0.0 2.2636277464171018E-4 30-31 0.25413465755556497 0.0 2.2636277464171018E-4 32-33 0.28922088762503007 0.0 2.2636277464171018E-4 34-35 0.32863630575951785 0.0 2.829534683021377E-4 36-37 0.3763139651684281 0.0 2.829534683021377E-4 38-39 0.4339798820084037 1.4147673415106886E-5 2.829534683021377E-4 40-41 0.49690873335879915 2.8295346830213772E-5 2.9710114171724463E-4 42-43 0.5658927889308603 2.8295346830213772E-5 3.5369183537767215E-4 44-45 0.6386967163250004 2.8295346830213772E-5 3.819871822078859E-4 46-47 0.7173153374927493 2.8295346830213772E-5 4.385778758683134E-4 48-49 0.801196893170918 2.8295346830213772E-5 4.5272554928342036E-4 50-51 0.8905818938075634 2.8295346830213772E-5 4.6687322269852724E-4 52-53 0.9834613697777401 2.8295346830213772E-5 4.810208961136341E-4 54-55 1.0834429778023003 2.8295346830213772E-5 4.810208961136341E-4 56-57 1.1878811029526193 2.8295346830213772E-5 5.093162429438479E-4 58-59 1.2968464835957727 2.8295346830213772E-5 5.093162429438479E-4 60-61 1.4093629302661177 2.8295346830213772E-5 5.093162429438479E-4 62-63 1.5217096048554817 2.8295346830213772E-5 5.376115897740616E-4 64-65 1.6402671080740774 2.8295346830213772E-5 5.376115897740616E-4 66-67 1.767313215341737 2.8295346830213772E-5 5.659069366042754E-4 68-69 1.8926333064527538 2.8295346830213772E-5 6.22497630264703E-4 70-71 2.0248008714966823 2.8295346830213772E-5 6.507929770949168E-4 72-73 2.162330404764936 2.8295346830213772E-5 6.507929770949168E-4 74-75 2.3048965097689686 2.8295346830213772E-5 6.507929770949168E-4 76-77 2.4524850388353636 2.8295346830213772E-5 6.507929770949168E-4 78-79 2.604133950171894 2.8295346830213772E-5 6.932359973402375E-4 80-81 2.7620785761781477 2.8295346830213772E-5 7.215313441704511E-4 82-83 2.9255690901631226 2.8295346830213772E-5 8.064173846610925E-4 84-85 3.0952987281241597 2.8295346830213772E-5 8.347127314913062E-4 86-87 3.271592886549807 2.8295346830213772E-5 8.77155751736627E-4 88-89 3.4597569429707287 2.8295346830213772E-5 9.054510985668407E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1865 0.0 78.94797 2 GAGCGGC 1350 0.0 70.64531 9 GATCGGG 2165 0.0 69.993645 1 TCGGGAG 2010 0.0 63.551838 3 AGAGCGG 1820 0.0 55.268715 8 CGGGAGA 2370 0.0 54.497944 4 GAGAGCG 2390 0.0 50.429127 7 TCGGGAA 615 0.0 50.18918 3 AAGCGGC 455 0.0 45.884113 9 GATCCGG 530 0.0 43.02206 1 AGCGGCG 1280 0.0 42.07393 10-11 AAAGCGG 680 0.0 39.074554 8 TCGGAGA 640 0.0 38.581837 4 ATCCGGA 590 0.0 37.835888 2 GGCGCCG 1340 0.0 37.74209 56-57 TCCGGAG 485 0.0 37.206062 3 TCGGAAA 620 0.0 35.99695 4 GCGTCGG 450 0.0 35.85037 10-11 CGCCGTA 3580 0.0 34.883987 58-59 CGCCGGA 730 0.0 34.800407 58-59 >>END_MODULE