FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534150
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT209140.5917688836070908Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA18650.078.947972
GAGCGGC13500.070.645319
GATCGGG21650.069.9936451
TCGGGAG20100.063.5518383
AGAGCGG18200.055.2687158
CGGGAGA23700.054.4979444
GAGAGCG23900.050.4291277
TCGGGAA6150.050.189183
AAGCGGC4550.045.8841139
GATCCGG5300.043.022061
AGCGGCG12800.042.0739310-11
AAAGCGG6800.039.0745548
TCGGAGA6400.038.5818374
ATCCGGA5900.037.8358882
GGCGCCG13400.037.7420956-57
TCCGGAG4850.037.2060623
TCGGAAA6200.035.996954
GCGTCGG4500.035.8503710-11
CGCCGTA35800.034.88398758-59
CGCCGGA7300.034.80040758-59
Produced by FastQC (version 0.11.2)