##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781806 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23537371046004 33.0 33.0 33.0 27.0 33.0 2 30.789223259997282 33.0 33.0 33.0 27.0 33.0 3 30.657461375811252 33.0 33.0 33.0 27.0 33.0 4 34.09093085571028 37.0 37.0 37.0 27.0 37.0 5 34.11410141469247 37.0 37.0 37.0 27.0 37.0 6 34.67180745170583 37.0 37.0 37.0 27.0 37.0 7 34.15543175908025 37.0 37.0 37.0 27.0 37.0 8 34.34857031726871 37.0 37.0 37.0 27.0 37.0 9 33.8751828847878 37.0 37.0 37.0 27.0 37.0 10-11 33.89871292246835 37.0 37.0 37.0 24.5 37.0 12-13 33.906231239705434 37.0 37.0 37.0 27.0 37.0 14-15 35.53053088533133 40.0 37.0 40.0 27.0 40.0 16-17 35.444726195859815 40.0 37.0 40.0 24.5 40.0 18-19 35.46489708484344 40.0 37.0 40.0 24.5 40.0 20-21 35.408002930470346 40.0 37.0 40.0 24.5 40.0 22-23 36.1650201344019 40.0 37.0 40.0 27.0 40.0 24-25 35.55376165699549 40.0 37.0 40.0 22.0 40.0 26-27 35.34960309956912 40.0 37.0 40.0 24.5 40.0 28-29 35.09854856880746 40.0 37.0 40.0 18.5 40.0 30-31 35.077579637113445 40.0 37.0 40.0 18.5 40.0 32-33 34.895614036349045 40.0 37.0 40.0 15.0 40.0 34-35 35.1291324772468 40.0 37.0 40.0 22.0 40.0 36-37 34.807269090655495 37.0 37.0 40.0 15.0 40.0 38-39 34.741171203168015 37.0 37.0 40.0 15.0 40.0 40-41 35.168431946728134 37.0 37.0 40.0 22.0 40.0 42-43 34.899342729147904 37.0 37.0 40.0 22.0 40.0 44-45 34.4632400677833 37.0 37.0 40.0 15.0 40.0 46-47 34.30418530264152 37.0 37.0 40.0 15.0 40.0 48-49 34.18740882721512 37.0 35.0 40.0 15.0 40.0 50-51 33.19895618170354 37.0 33.0 38.5 15.0 38.5 52-53 33.630562842987615 37.0 33.0 38.5 18.5 40.0 54-55 34.316790243460545 37.0 35.0 40.0 18.5 40.0 56-57 34.5929791653336 37.0 35.0 40.0 22.0 40.0 58-59 34.10337798538072 37.0 33.0 40.0 15.0 40.0 60-61 33.87086410770557 37.0 33.0 40.0 15.0 40.0 62-63 33.520481119100324 37.0 33.0 40.0 15.0 40.0 64-65 33.284863322604096 37.0 33.0 40.0 15.0 40.0 66-67 33.13060112746899 37.0 33.0 37.0 15.0 40.0 68-69 33.167159571875246 37.0 33.0 37.0 18.5 40.0 70-71 32.76792342816142 37.0 33.0 37.0 15.0 40.0 72-73 32.94761712355211 37.0 33.0 37.0 22.0 38.5 74-75 32.986163125681664 37.0 33.0 37.0 22.0 37.0 76-77 32.73020494599551 37.0 33.0 37.0 22.0 37.0 78-79 32.30869226682235 37.0 33.0 37.0 22.0 37.0 80-81 31.837144646319693 37.0 33.0 37.0 15.0 37.0 82-83 31.509900582571177 37.0 33.0 37.0 15.0 37.0 84-85 31.24120283729347 33.0 33.0 37.0 15.0 37.0 86-87 31.055240552360587 33.0 33.0 37.0 15.0 37.0 88-89 30.8773111784215 33.0 33.0 37.0 15.0 37.0 90-91 30.742012742800902 33.0 33.0 37.0 15.0 37.0 92-93 30.557145609722603 33.0 30.0 37.0 15.0 37.0 94-95 30.425645605768338 33.0 27.0 37.0 15.0 37.0 96-97 30.286962678202578 33.0 27.0 37.0 15.0 37.0 98-99 30.141392318515383 33.0 27.0 37.0 15.0 37.0 100-101 28.887219310045346 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.04864451314659135 2306 2 0.14297636957221727 2306 3 0.14606720301315335 2306 4 0.31810271429707626 2306 5 0.3909066109348629 2306 6 0.09782804441515225 2306 7 0.491251934325291 2306 8 -0.023830754313351576 2306 9 0.33894127461024226 2306 10-11 0.22042592837556896 2306 12-13 0.18940681016427163 2306 14-15 0.6025783597541547 2306 16-17 0.49098415965164577 2306 18-19 0.5068928784912927 2306 20-21 0.40863479874099795 2306 22-23 0.26596513438652636 2306 24-25 0.4700507775730145 2306 26-27 0.3361836277803505 2306 28-29 0.3811307707081184 2306 30-31 0.4416035286733191 2306 32-33 0.493332450105882 2306 34-35 0.281111803765711 2306 36-37 0.5026916845278535 2306 38-39 0.3375383168482742 2306 40-41 0.12358889491331126 2306 42-43 0.43895253555643166 2306 44-45 0.6623917967558484 2306 46-47 0.6304884580922447 2306 48-49 0.48660908888117405 2306 50-51 0.44178449586544133 2306 52-53 0.421033614630268 2306 54-55 0.48106893955188923 2306 56-57 0.30703638632116537 2306 58-59 0.6659159384122049 2306 60-61 0.5639781638696988 2306 62-63 0.6807534122348997 2306 64-65 0.5519007142978012 2306 66-67 0.5128222983649593 2306 68-69 0.21265634495567554 2306 70-71 0.43849253915590225 2306 72-73 0.005102297908656794 2306 74-75 -0.05653868622569291 2306 76-77 0.005483626746354275 2306 78-79 0.059440503564523794 2306 80-81 0.20925920170225254 2306 82-83 0.3963926872163164 2306 84-85 0.42186497794141076 2306 86-87 0.31236466147295516 2306 88-89 0.2862886647670315 2306 90-91 0.357578962161206 2306 92-93 0.41515175911021984 2306 94-95 0.34132469891753203 2306 96-97 0.24092525671260745 2306 98-99 0.23119020564766046 2306 100-101 0.2930597880602335 2307 1 -0.024835319569920244 2307 2 -0.044071724430821746 2307 3 -0.043910658948306036 2307 4 0.14325299260855218 2307 5 0.2419817517383933 2307 6 -0.0683061119336088 2307 7 0.3154073603280523 2307 8 -0.13261692739046538 2307 9 0.07475917091161932 2307 10-11 0.06388221007490102 2307 12-13 0.07697541995806745 2307 14-15 0.3498864912608539 2307 16-17 0.3429607437531246 2307 18-19 0.29526637117675847 2307 20-21 0.18281856670601115 2307 22-23 0.056677765838855976 2307 24-25 0.29630054276315576 2307 26-27 0.01625148371091001 2307 28-29 0.26397414099585603 2307 30-31 0.36698950253280316 2307 32-33 0.27218450588681975 2307 34-35 0.1372418447626842 2307 36-37 0.32358315046641906 2307 38-39 0.11742652263728814 2307 40-41 -0.017955519578002566 2307 42-43 0.3005300623577156 2307 44-45 0.5299849874346378 2307 46-47 0.40234987413577983 2307 48-49 0.19268955204156413 2307 50-51 -0.021147986824544773 2307 52-53 0.13500945640303286 2307 54-55 0.23383784215769765 2307 56-57 0.02736342290535987 2307 58-59 0.39665565950751613 2307 60-61 0.35090675201154653 2307 62-63 0.4173670873964639 2307 64-65 0.3840099124180085 2307 66-67 0.2889327430371722 2307 68-69 0.04334906288394791 2307 70-71 0.1551965007690228 2307 72-73 -0.236995114404273 2307 74-75 -0.20718177688371497 2307 76-77 0.02419910557721039 2307 78-79 0.12950756031764854 2307 80-81 0.32576330716731405 2307 82-83 0.4949631894952127 2307 84-85 0.5091939580921512 2307 86-87 0.6514421511989958 2307 88-89 0.38007409221984645 2307 90-91 0.4741420790520259 2307 92-93 0.5044369731744212 2307 94-95 0.24885750226715686 2307 96-97 0.25871859190244706 2307 98-99 0.3587678618003416 2307 100-101 0.04430798785129397 2201 1 0.18636582260911894 2201 2 0.16128718431846778 2201 3 0.17356845054760228 2201 4 0.4625841256402765 2201 5 0.46158081475601875 2201 6 0.3092526657864809 2201 7 0.6139746920297 2201 8 0.24388187356876756 2201 9 0.47350203229261467 2201 10-11 0.4607639315018801 2201 12-13 0.37891968928487785 2201 14-15 0.6896878460533102 2201 16-17 0.6160198737686926 2201 18-19 0.6068392777999136 2201 20-21 0.5194415363715095 2201 22-23 0.4483105935972702 2201 24-25 0.639583133185667 2201 26-27 0.4711082168810705 2201 28-29 0.5829352092546429 2201 30-31 0.603272383444569 2201 32-33 0.6730712707017688 2201 34-35 0.451527272090928 2201 36-37 0.620830146683879 2201 38-39 0.5384628894201526 2201 40-41 0.2882309745256819 2201 42-43 0.5841155650895473 2201 44-45 0.9084415005746465 2201 46-47 0.7325898059016964 2201 48-49 0.5099393604422673 2201 50-51 0.45725923585092687 2201 52-53 0.4276804742279481 2201 54-55 0.600934371120097 2201 56-57 0.6297886991791941 2201 58-59 0.8280036223743394 2201 60-61 0.7397621625284927 2201 62-63 0.7170106199930686 2201 64-65 0.6972214565847352 2201 66-67 0.6240723632997245 2201 68-69 0.5504034709039232 2201 70-71 0.6574462473635023 2201 72-73 0.3503942524527517 2201 74-75 0.24618233594972594 2201 76-77 0.5352523763626493 2201 78-79 0.6487061189964791 2201 80-81 0.8110449272320892 2201 82-83 0.7824826584561215 2201 84-85 0.731765127960383 2201 86-87 0.6875763881393411 2201 88-89 0.7104507702588556 2201 90-91 0.6977199178119484 2201 92-93 0.836634617121117 2201 94-95 0.7992303483202079 2201 96-97 0.5815085558076092 2201 98-99 0.41983472995530136 2201 100-101 0.4666623723849064 1101 1 -0.07781707647697544 1101 2 -0.12511076867192017 1101 3 -0.17419721750324157 1101 4 -0.01405407152412863 1101 5 0.007282662217129143 1101 6 -0.12001950614835266 1101 7 0.2015860920567718 1101 8 -0.20264502181454702 1101 9 -0.17923826646249807 1101 10-11 -0.12817351637315966 1101 12-13 -0.10033130518277744 1101 14-15 0.058811917628780463 1101 16-17 0.07130021321948021 1101 18-19 0.14227878665733584 1101 20-21 0.06031377511362024 1101 22-23 0.15670997006277076 1101 24-25 0.35307519927682307 1101 26-27 -0.025255590172548636 1101 28-29 0.027363461305810688 1101 30-31 0.19674961439249472 1101 32-33 0.16935047704890138 1101 34-35 -0.001959532382102225 1101 36-37 0.26236303507207737 1101 38-39 0.08129513549794609 1101 40-41 0.08241710481295428 1101 42-43 0.16816936895563828 1101 44-45 0.19072158240262382 1101 46-47 0.18478358409284112 1101 48-49 0.12376660907446535 1101 50-51 0.13125559856635505 1101 52-53 0.15968863301092284 1101 54-55 0.1866621773528223 1101 56-57 0.22608995629111206 1101 58-59 0.2417766719732839 1101 60-61 0.22417789367509044 1101 62-63 0.26259893876416385 1101 64-65 0.2787189545870703 1101 66-67 0.33192947652712235 1101 68-69 0.06096873461796548 1101 70-71 0.3298679453160531 1101 72-73 0.06228572775808772 1101 74-75 0.20170883616586366 1101 76-77 0.3659027756779807 1101 78-79 0.3884514217469288 1101 80-81 0.519051592676135 1101 82-83 0.41854433728345697 1101 84-85 0.38369489736614426 1101 86-87 0.2244759414476114 1101 88-89 0.4026668502878472 1101 90-91 0.3903221591302781 1101 92-93 0.42127789687764405 1101 94-95 0.32350283682543335 1101 96-97 0.1901497811314954 1101 98-99 0.26882847277788713 1101 100-101 0.21179128202916786 2304 1 0.06536295799542557 2304 2 0.023620567615992627 2304 3 0.009826077026403368 2304 4 0.1248359007569988 2304 5 0.16012324823424962 2304 6 0.16453346454736817 2304 7 0.3423596272705538 2304 8 -0.0018101027371031364 2304 9 0.04161363564355014 2304 10-11 0.07749381675079547 2304 12-13 0.178734682983837 2304 14-15 0.29424659284033083 2304 16-17 0.21604922773168767 2304 18-19 0.21602144724514716 2304 20-21 0.21369919338398802 2304 22-23 0.2246431108487883 2304 24-25 0.35964243790616734 2304 26-27 0.2546494567702666 2304 28-29 0.2977694391676522 2304 30-31 0.29683435847357487 2304 32-33 0.26132885276796003 2304 34-35 0.23231388890442872 2304 36-37 0.37202408269640586 2304 38-39 0.23867322479090802 2304 40-41 0.14669490870618773 2304 42-43 0.40047078829069704 2304 44-45 0.3885727537320989 2304 46-47 0.21103337967383595 2304 48-49 0.21641152761134919 2304 50-51 0.17342168054334195 2304 52-53 0.23251166465258422 2304 54-55 0.3140982703256654 2304 56-57 0.4011067545728295 2304 58-59 0.48183053253316643 2304 60-61 0.47350818621152513 2304 62-63 0.4234938903160028 2304 64-65 0.29138001794909485 2304 66-67 0.36196333310468987 2304 68-69 0.2550137779616435 2304 70-71 0.4440457121914818 2304 72-73 0.2627077888627767 2304 74-75 0.28237480079436494 2304 76-77 0.40505714128111947 2304 78-79 0.40149925613707893 2304 80-81 0.5457263916270421 2304 82-83 0.45625707845512053 2304 84-85 0.483558287307293 2304 86-87 0.3716187966354738 2304 88-89 0.4125996468972666 2304 90-91 0.40997717511088183 2304 92-93 0.43548946559083035 2304 94-95 0.3653742646246343 2304 96-97 0.39823746953055306 2304 98-99 0.4387947530498586 2304 100-101 0.5843179830861267 2202 1 -0.049020226963154556 2202 2 -0.1418860261809094 2202 3 -0.08777663000125102 2202 4 -0.18466835825401517 2202 5 0.08004002852122483 2202 6 0.011566160461242703 2202 7 0.1791489911368771 2202 8 -0.004148071269689524 2202 9 -0.062008719740397567 2202 10-11 -0.01990058638296688 2202 12-13 0.018979440854828056 2202 14-15 0.1319342573033424 2202 16-17 0.2111263176769569 2202 18-19 0.1498433478932597 2202 20-21 0.20279832757815797 2202 22-23 0.16196002938971787 2202 24-25 0.10311324862683335 2202 26-27 0.1324719824891929 2202 28-29 0.24382270895000602 2202 30-31 0.20573251521485503 2202 32-33 0.16555154984030906 2202 34-35 0.03377656733923118 2202 36-37 0.08544582344860174 2202 38-39 0.1193026812848359 2202 40-41 0.021884594146619918 2202 42-43 0.19968874959231897 2202 44-45 0.23566512417630747 2202 46-47 0.08374553774673643 2202 48-49 0.18671525346623952 2202 50-51 0.12100230003816392 2202 52-53 0.14601589947552185 2202 54-55 0.2561472995563179 2202 56-57 0.38907334574722796 2202 58-59 0.28945400948002487 2202 60-61 0.14374482198110172 2202 62-63 0.20015344696128778 2202 64-65 0.24217781072347577 2202 66-67 0.22302447674240256 2202 68-69 0.22019074556616403 2202 70-71 0.26249080716787887 2202 72-73 0.21076405414741117 2202 74-75 0.33703285141364603 2202 76-77 0.43698572025709836 2202 78-79 0.40576988379220325 2202 80-81 0.48760705911610813 2202 82-83 0.4435047108880248 2202 84-85 0.35053914435448874 2202 86-87 0.4096513639706245 2202 88-89 0.37543578159175084 2202 90-91 0.3450754741798967 2202 92-93 0.441530915042204 2202 94-95 0.207623291054567 2202 96-97 0.126591846490264 2202 98-99 0.27557997948207813 2202 100-101 0.45596873017941775 1102 1 -0.04526188542955367 1102 2 -0.17082677200566465 1102 3 -0.14784991329687003 1102 4 -0.14844629467751247 1102 5 -0.09499734357066814 1102 6 0.029831856510135424 1102 7 -0.11313460982687928 1102 8 0.07679416790740845 1102 9 -0.11218808434286132 1102 10-11 -0.06631119641249938 1102 12-13 -0.0653540802194783 1102 14-15 -0.20327434190704707 1102 16-17 -0.15171810508385875 1102 18-19 -0.05616692058858774 1102 20-21 -0.2730499293249906 1102 22-23 0.007981268314765089 1102 24-25 -0.08369215953827336 1102 26-27 -0.0036431330480724 1102 28-29 -0.14822638424271872 1102 30-31 -0.02350526255679597 1102 32-33 -0.08619776420071901 1102 34-35 -0.11459262819528249 1102 36-37 0.005942344727799309 1102 38-39 -0.14878936201993298 1102 40-41 0.06929654041410771 1102 42-43 0.15901342723984868 1102 44-45 -0.06257450558501176 1102 46-47 -0.1328634437447107 1102 48-49 -0.11775849449593778 1102 50-51 0.043155030536155436 1102 52-53 0.04747883962168231 1102 54-55 0.0744745244170204 1102 56-57 0.23216277813552466 1102 58-59 0.19687443318659348 1102 60-61 0.1830939365818054 1102 62-63 0.11081263216546944 1102 64-65 0.21216475154709258 1102 66-67 0.2174771597385572 1102 68-69 0.22895255249800783 1102 70-71 0.2296525985414064 1102 72-73 0.2916321004192852 1102 74-75 0.5038938437701717 1102 76-77 0.33964378636581216 1102 78-79 0.27385152937093693 1102 80-81 0.36285233978886566 1102 82-83 0.23223118698994583 1102 84-85 0.2772787421110259 1102 86-87 0.21386761890877892 1102 88-89 0.19947938221249117 1102 90-91 0.2656314876029171 1102 92-93 0.25411665034213016 1102 94-95 0.21887725987731343 1102 96-97 -0.04185912509224465 1102 98-99 0.20534257443598491 1102 100-101 0.2955920752335466 2305 1 0.13198512690103925 2305 2 0.023014019591798274 2305 3 0.03586283190368178 2305 4 0.04851159935622462 2305 5 0.07893514044150152 2305 6 0.18938113928064837 2305 7 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-0.3727690918432103 1213 98-99 -0.23889628150721265 1213 100-101 -0.0301949944423896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 379.0 3 214.0 4 226.0 5 166.0 6 465.0 7 3076.0 8 16577.0 9 47869.0 10 62290.0 11 43827.0 12 24538.0 13 15333.0 14 11231.0 15 9164.0 16 8096.0 17 7651.0 18 7573.0 19 7970.0 20 8598.0 21 9492.0 22 10836.0 23 11498.0 24 13028.0 25 15578.0 26 18728.0 27 22774.0 28 28291.0 29 34228.0 30 40960.0 31 52930.0 32 72545.0 33 103459.0 34 157329.0 35 266329.0 36 512006.0 37 864379.0 38 271339.0 39 834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.365576717327635 23.83703714377317 19.92867358076636 14.868712558132833 2 32.744491811733994 26.066520386743754 27.778622192959833 13.410365608562419 3 36.96514980090642 20.489138615080698 26.11654806513014 16.429163518882742 4 30.588941810047643 18.74339110576499 30.067935022305974 20.599732061881394 5 35.434295962975916 16.8912858510618 28.627702748684925 19.046715437277363 6 33.523625242633884 17.052127457787194 31.79645712241345 17.627790177165476 7 36.79208751724728 18.75353133014095 25.01110232202276 19.443278830589 8 29.82423057919376 30.117809379623157 21.52737267845852 18.53058736272456 9 32.61948248962273 25.941366287270363 22.27456432753588 19.164586895571023 10-11 27.40828831966314 29.97980590935289 26.357569599996545 16.254336170987425 12-13 29.166156853543768 25.326742229368403 26.24609723059915 19.26100368648868 14-15 26.638005502526084 25.40656744175 27.80351832762935 20.15190872809457 16-17 27.975637782846093 25.87111945981841 28.347893295748563 17.805349461586943 18-19 27.457780979204955 26.017138749246215 28.69770835702022 17.827371914528612 20-21 25.86640875225086 28.00647232772258 27.97363933324374 18.153479586782822 22-23 28.4892167084356 26.02055060156614 27.15165755377255 18.338575136225714 24-25 25.271169558564544 28.92522807979906 27.347399457227823 18.45620290440858 26-27 23.366190292085932 30.816836875905214 27.459783554281508 18.357189277727347 28-29 24.548016937693998 29.79736723846435 27.078596786385944 18.576019037455705 30-31 26.544597087931006 27.58540105403704 27.370539102930646 18.499462755101305 32-33 23.29091218525192 29.76705738065844 28.770597128760926 18.171433305328712 34-35 27.21933782977988 27.634344562563562 27.37196680306324 17.77435080459331 36-37 24.167108522094452 29.007334559962423 29.2587418935138 17.56681502442932 38-39 23.205257927252514 31.355805213765393 27.453495865318565 17.98544099366353 40-41 27.57875707175209 26.876322928442413 26.91291873336012 18.632001266445375 42-43 27.44333666567597 26.800050435456125 27.21070312429638 18.545909774571523 44-45 24.551248260039632 28.38950763151255 28.453460011032732 18.605784097415086 46-47 24.847489335916862 28.926022668505663 27.5744072091922 18.652080786385277 48-49 21.706326635223988 29.38556376904365 28.99906233913785 19.909047256594516 50-51 20.338898182850407 27.931920651182494 30.37329664740151 21.35588451856559 52-53 25.38814620945325 27.242095245710967 27.209136632004288 20.16062191283149 54-55 26.772325472097325 26.977064542326247 27.332557833835423 18.918052151741 56-57 27.287916592006585 26.907829989311978 26.669686085491573 19.134567333189867 58-59 25.850977259553126 27.28516883768428 26.765291497763556 20.098562404999036 60-61 22.30432939917447 27.33059933478063 26.73955729100089 23.62551397504401 62-63 24.612849074011567 27.396728099192153 26.710051981367716 21.280370845428564 64-65 22.490337828544433 28.51114705295669 28.81150790364545 20.187007214853423 66-67 21.152255475969188 28.5032356894876 27.869097445910096 22.47541138863312 68-69 20.842106362328153 27.325761117839477 32.19481246407147 19.637320055760906 70-71 20.200331605695265 30.876990849339037 29.848131072461786 19.07454647250391 72-73 19.93313345260886 34.300241635560575 26.59026335791696 19.17636155391361 74-75 20.23652676447753 34.2580560032388 26.24751514047433 19.25790209180934 76-77 20.484159889964477 33.68379550532264 26.200858032214914 19.63118657249796 78-79 20.209553402812407 33.572795503603274 26.645759334037844 19.57189175954647 80-81 19.61600657350599 33.51490651225745 26.845512887218785 20.02357402701777 82-83 19.567838739896125 33.04578047514652 26.815960768339508 20.570420016617845 84-85 19.66974378293518 32.44056564003484 27.369527491008245 20.520163086021736 86-87 19.98573000976785 32.04043211921961 27.888196319336593 20.085641551675952 88-89 20.450621786386435 32.239007302950895 27.52639778119963 19.78397312946304 90-91 20.883821373130633 31.643529184882514 26.89065340810362 20.581996033883236 92-93 20.746614558438395 31.835005733093315 26.71252421194496 20.705855496523327 94-95 20.305750441531504 31.952640457428444 27.04344021906444 20.69816888197561 96-97 20.62407601492744 32.280435219812794 26.700182046777616 20.39530671848215 98-99 20.970261503640984 31.663205365739728 26.60153670596637 20.764996424652914 100-101 21.134779233923613 31.65470554017768 26.266417659125 20.9440975667737 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 141.0 1 160.5 2 173.5 3 148.0 4 112.0 5 122.5 6 166.0 7 200.5 8 259.5 9 359.0 10 450.5 11 528.0 12 603.0 13 620.5 14 584.0 15 561.0 16 537.5 17 491.0 18 453.5 19 471.5 20 566.5 21 679.5 22 904.5 23 1279.0 24 1778.0 25 2467.5 26 3445.5 27 4662.0 28 6249.0 29 8245.0 30 10608.0 31 13989.0 32 19756.0 33 28981.0 34 42157.0 35 60930.0 36 87478.5 37 119840.5 38 151022.5 39 170881.0 40 177753.0 41 170757.5 42 159630.0 43 156432.0 44 149605.0 45 142422.0 46 144224.5 47 138249.0 48 118895.5 49 99647.0 50 84823.0 51 75316.0 52 68275.0 53 59747.5 54 51111.5 55 43414.5 56 36413.5 57 29876.0 58 24367.5 59 20074.5 60 16539.0 61 13593.0 62 11282.0 63 9225.5 64 7435.5 65 6098.5 66 4948.5 67 3953.0 68 3226.0 69 2616.5 70 2007.0 71 1596.5 72 1257.0 73 945.0 74 733.5 75 572.0 76 432.5 77 304.5 78 231.0 79 171.5 80 127.5 81 108.5 82 76.0 83 58.0 84 50.5 85 45.5 86 31.5 87 21.5 88 20.0 89 17.0 90 15.0 91 10.0 92 7.5 93 9.0 94 5.0 95 1.5 96 2.0 97 2.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20979176836918176 2 0.19390280990119368 3 0.16967394563100374 4 0.15453989242959432 5 0.1648928789426725 6 0.12376851584905635 7 0.11172597945363551 8 0.10766387016204582 9 0.09540564654760253 10-11 0.0819611432285357 12-13 0.10820308820960196 14-15 0.10426679646244202 16-17 0.09585499492056598 18-19 0.0895101958943219 20-21 0.09308700894311106 22-23 0.0800918539970077 24-25 0.38989059625293787 26-27 0.18601225247195527 28-29 0.08782064601197927 30-31 0.16802034361849819 32-33 0.1969404049024267 34-35 0.1964730825945447 36-37 0.20253029866209218 38-39 0.2032852039286708 40-41 0.19884564200379176 42-43 0.2150042094955579 44-45 0.19848616330542102 46-47 0.21628035887477415 48-49 0.20666430369335603 50-51 0.20885712375341772 52-53 0.2012002274781203 54-55 0.1833700840389301 56-57 0.19172796377605053 58-59 0.18655147051951143 60-61 0.16812818722800943 62-63 0.15703826938327115 64-65 0.16104645687010524 66-67 0.1728733060465036 68-69 0.16505464435693934 70-71 0.15856605385134692 72-73 0.16115430047961649 74-75 0.15222125482510285 76-77 0.1625203195334254 78-79 0.16072292604157157 80-81 0.16525235764104326 82-83 0.18955311765090738 84-85 0.1881691246621799 86-87 0.1923211036283623 88-89 0.19882766806887325 90-91 0.18097955069476448 92-93 0.16210691903029903 94-95 0.16323927693016696 96-97 0.1715612087974503 98-99 0.18522139933553958 100-101 0.19595183848190706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2781806.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.74249847473641 #Duplication Level Percentage of deduplicated Percentage of total 1 91.39682267537155 54.6027453927914 2 4.392365956374142 5.248218328983327 3 1.187406560664117 2.1281590391810403 4 0.6433728431433415 1.5374680440071165 5 0.4334914948684278 1.294893248549413 6 0.267281361263027 0.9580833790549116 7 0.24064929542618962 1.0063893115461866 8 0.1618511864682469 0.7735515408570814 9 0.11571048113374412 0.6221549918379352 >10 0.9409948409921216 11.025917792952109 >50 0.11531481191667972 4.754331676069154 >100 0.09601531980639613 11.607427816149526 >500 0.006749961477759136 2.6554952167644865 >1k 0.001973211094125681 1.7851642212562784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2828 0.10166057589925395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004134005031263863 0.0 0.0 2 0.004673223078820018 0.0 0.0 3 0.005500024085072791 0.0 0.0 4 0.006147085742140178 0.0 0.0 5 0.007585000535623261 0.0 0.0 6 0.008915071719595113 0.0 0.0 7 0.011323578998679276 0.0 0.0 8 0.013552346928578053 0.0 0.0 9 0.016320332906032988 0.0 0.0 10-11 0.026008283827125257 0.0 0.0 12-13 0.03566028687838045 0.0 0.0 14-15 0.045222420255042944 0.0 0.0 16-17 0.05446102280317175 0.0 0.0 18-19 0.06357380780687079 0.0 0.0 20-21 0.07060161636001935 0.0 3.594786983707706E-5 22-23 0.07804282541629431 0.0 3.594786983707706E-5 24-25 0.08634678334865911 0.0 3.594786983707706E-5 26-27 0.09434518438740876 0.0 3.594786983707706E-5 28-29 0.10378150021964148 0.0 3.594786983707706E-5 30-31 0.2012361753479574 0.0 3.594786983707706E-5 32-33 0.21435714783849052 0.0 5.3921804755615595E-5 34-35 0.22393725515007157 0.0 7.189573967415412E-5 36-37 0.23517096447415814 0.0 1.0784360951123119E-4 38-39 0.24940632092964066 0.0 1.0784360951123119E-4 40-41 0.26427076510727204 0.0 1.0784360951123119E-4 42-43 0.28269404839877404 0.0 1.0784360951123119E-4 44-45 0.30332812568525624 0.0 1.0784360951123119E-4 46-47 0.32527430022079185 0.0 1.0784360951123119E-4 48-49 0.35158814094153223 0.0 1.0784360951123119E-4 50-51 0.3804902282905422 0.0 1.0784360951123119E-4 52-53 0.4134903728009789 3.594786983707706E-5 1.0784360951123119E-4 54-55 0.44877320704607004 3.594786983707706E-5 1.2581754442976973E-4 56-57 0.48651847037500096 3.594786983707706E-5 1.4379147934830825E-4 58-59 0.5282898951256845 3.594786983707706E-5 1.4379147934830825E-4 60-61 0.5737459765346684 3.594786983707706E-5 1.7973934918538533E-4 62-63 0.6210713471751805 3.594786983707706E-5 1.7973934918538533E-4 64-65 0.6752088391498186 3.594786983707706E-5 1.7973934918538533E-4 66-67 0.7296878358879089 3.594786983707706E-5 1.9771328410392384E-4 68-69 0.7880132546985663 3.594786983707706E-5 2.1568721902246238E-4 70-71 0.8504726785404877 3.594786983707706E-5 2.1568721902246238E-4 72-73 0.9157719840995382 3.594786983707706E-5 2.1568721902246238E-4 74-75 0.9879732806673075 3.594786983707706E-5 2.1568721902246238E-4 76-77 1.0684965091023602 3.594786983707706E-5 2.1568721902246238E-4 78-79 1.1551847972144715 3.594786983707706E-5 2.1568721902246238E-4 80-81 1.2478584056544562 3.594786983707706E-5 2.1568721902246238E-4 82-83 1.347649692322182 3.594786983707706E-5 2.1568721902246238E-4 84-85 1.4547204226319161 3.594786983707706E-5 2.1568721902246238E-4 86-87 1.570364719897793 3.594786983707706E-5 2.1568721902246238E-4 88-89 1.688381576572917 3.594786983707706E-5 2.3366115394100092E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2575 0.0 84.9621 9 CGGGAGA 3590 0.0 82.53868 4 AGAGCGG 2715 0.0 82.33044 8 ATCGGGA 4255 0.0 80.47876 2 GAGAGCG 3205 0.0 79.073395 7 AAGCGGC 685 0.0 78.97976 9 TCGGGAG 3805 0.0 77.88048 3 CGGAAAG 1530 0.0 76.66072 5 GTTCGGA 455 0.0 75.15653 2 TCGGAGA 835 0.0 72.79321 4 ATTCGGA 545 0.0 72.33145 2 GATCGGG 4960 0.0 71.92667 1 GATCCGG 720 0.0 69.2769 1 ATCCGGA 720 0.0 65.96493 2 GAACCGG 340 0.0 65.66752 1 GGATCGG 1485 0.0 65.57813 1 TCGGAAA 1190 0.0 65.44316 4 TTCGGAG 545 0.0 65.352684 3 AAAGCGG 900 0.0 64.85925 8 CCGGAAA 730 0.0 64.399086 4 >>END_MODULE