FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781806
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC28280.10166057589925395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC25750.084.96219
CGGGAGA35900.082.538684
AGAGCGG27150.082.330448
ATCGGGA42550.080.478762
GAGAGCG32050.079.0733957
AAGCGGC6850.078.979769
TCGGGAG38050.077.880483
CGGAAAG15300.076.660725
GTTCGGA4550.075.156532
TCGGAGA8350.072.793214
ATTCGGA5450.072.331452
GATCGGG49600.071.926671
GATCCGG7200.069.27691
ATCCGGA7200.065.964932
GAACCGG3400.065.667521
GGATCGG14850.065.578131
TCGGAAA11900.065.443164
TTCGGAG5450.065.3526843
AAAGCGG9000.064.859258
CCGGAAA7300.064.3990864
Produced by FastQC (version 0.11.2)