##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347602 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.100745356325305 33.0 33.0 33.0 27.0 33.0 2 30.699812404317257 33.0 33.0 33.0 27.0 33.0 3 30.53540336053556 33.0 33.0 33.0 27.0 33.0 4 34.00643081748951 37.0 37.0 37.0 27.0 37.0 5 34.042272071671434 37.0 37.0 37.0 27.0 37.0 6 34.527909330457206 37.0 37.0 37.0 27.0 37.0 7 33.98915616872025 37.0 37.0 37.0 27.0 37.0 8 34.21800714090378 37.0 37.0 37.0 27.0 37.0 9 33.74695455192149 37.0 37.0 37.0 22.0 37.0 10-11 33.76074330316638 37.0 37.0 37.0 22.0 37.0 12-13 33.75585512365384 37.0 37.0 37.0 24.5 37.0 14-15 35.28953800516442 40.0 37.0 40.0 22.0 40.0 16-17 35.18943585837804 40.0 37.0 40.0 22.0 40.0 18-19 35.20753070580107 40.0 37.0 40.0 22.0 40.0 20-21 35.1961646394917 40.0 37.0 40.0 22.0 40.0 22-23 35.833760151848566 40.0 37.0 40.0 27.0 40.0 24-25 35.26481703457401 38.5 37.0 40.0 22.0 40.0 26-27 35.0764090335585 37.0 37.0 40.0 21.0 40.0 28-29 34.83943786042097 37.0 37.0 40.0 18.5 40.0 30-31 34.84199515079643 37.0 37.0 40.0 18.5 40.0 32-33 34.66079407838296 37.0 37.0 40.0 15.0 40.0 34-35 34.73132370819245 37.0 37.0 40.0 18.5 40.0 36-37 34.57919549395511 37.0 37.0 40.0 15.0 40.0 38-39 34.714595574547985 37.0 37.0 40.0 18.5 40.0 40-41 34.358657046637376 37.0 37.0 40.0 15.0 40.0 42-43 34.22577272467821 37.0 33.0 40.0 15.0 40.0 44-45 34.109746030204434 37.0 33.0 40.0 15.0 40.0 46-47 34.03875656946961 37.0 33.0 40.0 15.0 40.0 48-49 33.92850789869833 37.0 33.0 40.0 15.0 40.0 50-51 32.91381077371718 37.0 33.0 38.5 15.0 38.5 52-53 33.37206860447384 37.0 33.0 38.5 18.5 40.0 54-55 34.041346233305305 37.0 33.0 40.0 18.5 40.0 56-57 34.25571221186556 37.0 33.0 40.0 18.5 40.0 58-59 33.76331358552258 37.0 33.0 40.0 15.0 40.0 60-61 33.57775743077404 37.0 33.0 40.0 15.0 40.0 62-63 33.23037231183139 37.0 33.0 40.0 15.0 40.0 64-65 32.99278668189923 37.0 33.0 37.0 15.0 40.0 66-67 32.82997245700081 37.0 33.0 37.0 15.0 40.0 68-69 32.8614028272254 37.0 33.0 37.0 18.5 40.0 70-71 32.45064900268444 37.0 33.0 37.0 15.0 38.5 72-73 32.632061141539324 37.0 33.0 37.0 22.0 37.0 74-75 32.62106289737358 37.0 33.0 37.0 22.0 37.0 76-77 32.32093749281181 37.0 33.0 37.0 22.0 37.0 78-79 31.886710353799323 35.0 33.0 37.0 22.0 37.0 80-81 31.44133779916698 33.0 33.0 37.0 15.0 37.0 82-83 31.157082205586807 33.0 33.0 37.0 15.0 37.0 84-85 30.940329110300638 33.0 33.0 37.0 15.0 37.0 86-87 30.774868355027813 33.0 33.0 37.0 15.0 37.0 88-89 30.612048805547104 33.0 27.0 37.0 15.0 37.0 90-91 30.483682285157364 33.0 27.0 37.0 15.0 37.0 92-93 30.292500602742713 33.0 27.0 37.0 15.0 37.0 94-95 30.15735035155022 33.0 27.0 37.0 15.0 37.0 96-97 30.005405515926462 33.0 27.0 37.0 15.0 37.0 98-99 29.839660853926688 33.0 27.0 37.0 15.0 37.0 100-101 28.581189017559197 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.0657559937043466 2306 2 -0.053240471705283454 2306 3 -0.08095482101916218 2306 4 -0.03145710418571923 2306 5 0.03671076403499285 2306 6 -0.23475009611032505 2306 7 0.19393441005002643 2306 8 -0.19783707483774293 2306 9 0.0112196861608993 2306 10-11 0.06330933818022544 2306 12-13 0.05884611551798713 2306 14-15 0.395266759104544 2306 16-17 0.30532049614704704 2306 18-19 0.30413958897988635 2306 20-21 0.17852424026220604 2306 22-23 0.0513728868371075 2306 24-25 0.2975514035669491 2306 26-27 0.11009135246435164 2306 28-29 0.3158506153755596 2306 30-31 0.20382554580954348 2306 32-33 0.27203790784363235 2306 34-35 0.14999202366539066 2306 36-37 0.2325897932808303 2306 38-39 0.03836632615681168 2306 40-41 0.21584067088121373 2306 42-43 0.26311597135376275 2306 44-45 0.3421841231127871 2306 46-47 0.32256164964295664 2306 48-49 0.34923007193521727 2306 50-51 0.420380674883603 2306 52-53 0.21594795983386206 2306 54-55 0.36540310891097505 2306 56-57 0.18704469764369946 2306 58-59 0.3302346768282547 2306 60-61 0.24890294436932692 2306 62-63 0.3632230806805836 2306 64-65 0.3046798829749875 2306 66-67 0.34787599962394467 2306 68-69 0.2544802261255086 2306 70-71 0.41766346393276876 2306 72-73 0.039719941235645706 2306 74-75 0.030155832082101597 2306 76-77 0.1738648695434435 2306 78-79 0.14675146753687685 2306 80-81 0.39374594877643077 2306 82-83 0.14569295739774546 2306 84-85 0.271921456155237 2306 86-87 0.15234051111893265 2306 88-89 0.46781366644930245 2306 90-91 0.39284283002186626 2306 92-93 0.2998263441070037 2306 94-95 0.18966270701818644 2306 96-97 -0.028805636298681492 2306 98-99 0.2664358603768413 2306 100-101 0.2407237463815335 2307 1 -0.09440226096968019 2307 2 -0.1771157199612965 2307 3 -0.16924839586074825 2307 4 -0.04295231470231187 2307 5 -0.053181557029276405 2307 6 -0.26928808096951684 2307 7 0.1252372981513048 2307 8 -0.1818681895916967 2307 9 -0.05753934900701552 2307 10-11 -0.09088692030326229 2307 12-13 -0.07293391951932193 2307 14-15 0.2609399888871664 2307 16-17 0.12646808068920024 2307 18-19 0.08393228113342133 2307 20-21 0.2622447816742337 2307 22-23 0.05929202855808313 2307 24-25 0.187377804496478 2307 26-27 -0.053161184137927364 2307 28-29 0.05556713064921581 2307 30-31 0.20695604853641925 2307 32-33 0.2740219856823103 2307 34-35 0.06247277115805616 2307 36-37 -0.07088742345962373 2307 38-39 -0.21199789525378065 2307 40-41 -0.04842673180917956 2307 42-43 0.07859279178529022 2307 44-45 -0.06617197171312483 2307 46-47 -0.0021434267140634233 2307 48-49 -0.06355438104588274 2307 50-51 -0.14711371824584774 2307 52-53 -0.09115373843993524 2307 54-55 -0.0321791814843877 2307 56-57 -0.013842831685416002 2307 58-59 0.06045919934354771 2307 60-61 0.0034855477586717143 2307 62-63 0.047302664466144506 2307 64-65 0.04094445746080311 2307 66-67 -0.008419232509496055 2307 68-69 0.05710543497580289 2307 70-71 -0.003667526073940053 2307 72-73 -0.16979800300188685 2307 74-75 -0.29415828327081783 2307 76-77 -0.08964903954869641 2307 78-79 -0.05069960182168742 2307 80-81 0.03270711275375149 2307 82-83 0.18941219279173183 2307 84-85 0.05825971933138163 2307 86-87 0.16773885788023435 2307 88-89 -0.025306939813280138 2307 90-91 -0.03380708733871529 2307 92-93 0.16212081514718335 2307 94-95 0.13629132277416645 2307 96-97 0.01951337211837867 2307 98-99 -0.008660796216975797 2307 100-101 -0.16518384608679426 2201 1 0.17187404214934432 2201 2 0.12699054615091399 2201 3 0.20981771116968062 2201 4 0.3184254440754657 2201 5 0.29990421499668685 2201 6 0.14872100016478385 2201 7 0.48615301157954605 2201 8 0.1575780684887036 2201 9 0.20671280684477011 2201 10-11 0.2467327472831755 2201 12-13 0.2849285701710116 2201 14-15 0.49809663953337946 2201 16-17 0.5015112624975657 2201 18-19 0.5144422259038919 2201 20-21 0.43485272539970055 2201 22-23 0.42680781150904323 2201 24-25 0.5457476804351415 2201 26-27 0.3127938463268407 2201 28-29 0.38378827764441326 2201 30-31 0.4323839802573417 2201 32-33 0.48579124153020103 2201 34-35 0.3560480737545788 2201 36-37 0.4234676346415043 2201 38-39 0.3419801748648368 2201 40-41 0.46280944461441464 2201 42-43 0.4648956301255538 2201 44-45 0.49155535891701874 2201 46-47 0.3831751362817144 2201 48-49 0.2904732941751007 2201 50-51 0.1651074892038764 2201 52-53 0.3820406142431594 2201 54-55 0.4300666935733659 2201 56-57 0.5214885239211 2201 58-59 0.7403443573241972 2201 60-61 0.5829681433308096 2201 62-63 0.665820265371238 2201 64-65 0.4788252670333435 2201 66-67 0.46141341639216904 2201 68-69 0.3494891895476755 2201 70-71 0.5884508762855134 2201 72-73 0.34479420959079476 2201 74-75 0.3770667630416682 2201 76-77 0.41288357351207594 2201 78-79 0.636300028973853 2201 80-81 0.6901989945737732 2201 82-83 0.543661174780599 2201 84-85 0.5330528784693023 2201 86-87 0.4240304473594172 2201 88-89 0.2707111037053487 2201 90-91 0.23123961378703228 2201 92-93 0.39688519360982255 2201 94-95 0.265377131504664 2201 96-97 0.2831148320953183 2201 98-99 0.2517908207258408 2201 100-101 0.21843551274971418 1101 1 0.08443008220993775 1101 2 0.009768691907201088 1101 3 0.048491332630188566 1101 4 0.16542304608217506 1101 5 0.21960670437130148 1101 6 -0.06235377306846601 1101 7 0.41387896571716 1101 8 0.02968483745339512 1101 9 0.195389801441614 1101 10-11 0.16436305767305015 1101 12-13 0.18612902339787496 1101 14-15 0.2849481540715999 1101 16-17 0.2783882595476044 1101 18-19 0.3741186041367328 1101 20-21 0.3174560813637157 1101 22-23 0.28696639491443676 1101 24-25 0.32750384254193676 1101 26-27 0.2166517943560038 1101 28-29 0.3785744821300696 1101 30-31 0.3256295813809018 1101 32-33 0.29935991245315563 1101 34-35 0.16728506418100864 1101 36-37 0.289739644356132 1101 38-39 0.18094626257865087 1101 40-41 0.41061207627406304 1101 42-43 0.2663275265027991 1101 44-45 0.3930241298074648 1101 46-47 0.4311499512191901 1101 48-49 0.2571582064428952 1101 50-51 0.3243678217337873 1101 52-53 0.3676104902957391 1101 54-55 0.4676553150645404 1101 56-57 0.5855336503523816 1101 58-59 0.6726386113397638 1101 60-61 0.4291531261278081 1101 62-63 0.46182026230474804 1101 64-65 0.45416917361585973 1101 66-67 0.4770103388652345 1101 68-69 0.3622883254114768 1101 70-71 0.45069313800409105 1101 72-73 0.20299982371626157 1101 74-75 0.3476721796853397 1101 76-77 0.45243666361161416 1101 78-79 0.593781262069843 1101 80-81 0.7004859104845558 1101 82-83 0.6538423735319263 1101 84-85 0.4357831256895288 1101 86-87 0.48007970741596395 1101 88-89 0.6706642631075042 1101 90-91 0.6322625940449846 1101 92-93 0.7139140124601369 1101 94-95 0.49263797613800264 1101 96-97 0.6643118201912124 1101 98-99 0.5165505920885245 1101 100-101 0.567819992029456 2304 1 0.16659113989770447 2304 2 0.15641128501398427 2304 3 0.24118653246245714 2304 4 0.22544167027209028 2304 5 0.2591177060542833 2304 6 0.11287510991458305 2304 7 0.3855783663809049 2304 8 0.2664125049530526 2304 9 0.38770061763823804 2304 10-11 0.3749658599903043 2304 12-13 0.3587571780353258 2304 14-15 0.47782562919054783 2304 16-17 0.38169147968137196 2304 18-19 0.4113092555867226 2304 20-21 0.4161229690273629 2304 22-23 0.3089947883041759 2304 24-25 0.49359735731946586 2304 26-27 0.4084092917257607 2304 28-29 0.4834896107899809 2304 30-31 0.49871755612302593 2304 32-33 0.5116336502676333 2304 34-35 0.42576145528144593 2304 36-37 0.4491770417672569 2304 38-39 0.2682503402562517 2304 40-41 0.5591495532063178 2304 42-43 0.349988105363785 2304 44-45 0.44799830904924676 2304 46-47 0.559015002341269 2304 48-49 0.5190639465088864 2304 50-51 0.3446899814976092 2304 52-53 0.33294052661857165 2304 54-55 0.5306208017584382 2304 56-57 0.5767673187700169 2304 58-59 0.7341128406364064 2304 60-61 0.7083935528975402 2304 62-63 0.7186160374847148 2304 64-65 0.5739840083686758 2304 66-67 0.618147014164002 2304 68-69 0.5175944251170037 2304 70-71 0.684076101214707 2304 72-73 0.4903820767143614 2304 74-75 0.5036275760726951 2304 76-77 0.4701847752207513 2304 78-79 0.5172586967671187 2304 80-81 0.5888097145036788 2304 82-83 0.528986989611063 2304 84-85 0.6233560830257794 2304 86-87 0.6663593838159407 2304 88-89 0.583544585687541 2304 90-91 0.5782183257055422 2304 92-93 0.5819305315121497 2304 94-95 0.5982019292639791 2304 96-97 0.4562799966261366 2304 98-99 0.5939501801274893 2304 100-101 0.6523278440332554 2202 1 0.12766958918539117 2202 2 0.10497769842950078 2202 3 0.09891198104029186 2202 4 0.2031287446239105 2202 5 0.3422469273948465 2202 6 0.2446881163875929 2202 7 0.3330395880307151 2202 8 0.20834425365106313 2202 9 0.07032081822439551 2202 10-11 0.08163496311576779 2202 12-13 0.07907074325393637 2202 14-15 0.27252176295213815 2202 16-17 0.23946129406538574 2202 18-19 0.38112952575252024 2202 20-21 0.3190092941681044 2202 22-23 0.46480662106001347 2202 24-25 0.4130092863622892 2202 26-27 0.3305516945426845 2202 28-29 0.2544553094328208 2202 30-31 0.40308200936058824 2202 32-33 0.40420602363619906 2202 34-35 0.30261383750560356 2202 36-37 0.2815151461086032 2202 38-39 0.20240050225734052 2202 40-41 0.26177521825415084 2202 42-43 0.2212253578816501 2202 44-45 0.2900428611039487 2202 46-47 0.3779555117485742 2202 48-49 0.243944993436493 2202 50-51 0.172313889483803 2202 52-53 0.29811776142526014 2202 54-55 0.4431630590906579 2202 56-57 0.5005130403249538 2202 58-59 0.5777853102919508 2202 60-61 0.5663522514068475 2202 62-63 0.5335943729148056 2202 64-65 0.495366042764914 2202 66-67 0.5592612899383411 2202 68-69 0.49581125355047817 2202 70-71 0.6127490628475059 2202 72-73 0.5112963813045113 2202 74-75 0.5160526373941465 2202 76-77 0.6269361966836087 2202 78-79 0.6879062794017337 2202 80-81 0.6364181779181415 2202 82-83 0.6062120446070942 2202 84-85 0.497498197684596 2202 86-87 0.6234563314484767 2202 88-89 0.5479302642295991 2202 90-91 0.45154779936016 2202 92-93 0.590634575565204 2202 94-95 0.6029982682503636 2202 96-97 0.5145863297790747 2202 98-99 0.5717097332715717 2202 100-101 0.5612615360414388 1102 1 0.202426940270918 1102 2 0.1357828562962382 1102 3 0.27439335692613653 1102 4 0.3138622502953794 1102 5 0.40242232351110374 1102 6 0.3728964363635896 1102 7 0.3351139548926838 1102 8 0.3726992474782662 1102 9 0.2984645113988478 1102 10-11 0.2638561129612782 1102 12-13 0.2666027156161732 1102 14-15 0.19712325942921183 1102 16-17 0.27432438414729887 1102 18-19 0.37048997619621105 1102 20-21 0.14432211176023912 1102 22-23 0.3186758737081803 1102 24-25 0.2259995292499255 1102 26-27 0.3705636138393231 1102 28-29 0.26643599301045384 1102 30-31 0.299908049016544 1102 32-33 0.325100004988208 1102 34-35 0.4334686164785353 1102 36-37 0.22449606159243984 1102 38-39 0.31851218555531347 1102 40-41 0.2748386991662173 1102 42-43 0.3828976573680478 1102 44-45 0.49307673987157585 1102 46-47 0.476511810389205 1102 48-49 0.38836841664484467 1102 50-51 0.3228926318701184 1102 52-53 0.1401913438986142 1102 54-55 0.43642840070895517 1102 56-57 0.5569263551512549 1102 58-59 0.5304478833594644 1102 60-61 0.394892998690473 1102 62-63 0.355910314419873 1102 64-65 0.3260114240801002 1102 66-67 0.4754237879773697 1102 68-69 0.38531519595589003 1102 70-71 0.41468411546976114 1102 72-73 0.2575449574686388 1102 74-75 0.3653958824439769 1102 76-77 0.4288367191546385 1102 78-79 0.3855398130383598 1102 80-81 0.2860697217995707 1102 82-83 0.5061961010557887 1102 84-85 0.44167701100692014 1102 86-87 0.4081190613937089 1102 88-89 0.22623799693724322 1102 90-91 0.4933062622312967 1102 92-93 0.4671202246638373 1102 94-95 0.4520324471318915 1102 96-97 0.2526606386644943 1102 98-99 0.4008519872634295 1102 100-101 0.2684693001246252 2305 1 0.13812389895356603 2305 2 -0.05965457505880423 2305 3 0.06238730431743278 2305 4 0.0023804673833325296 2305 5 0.12433517700794283 2305 6 0.1418416547236916 2305 7 0.2335776906007041 2305 8 0.16348324204198406 2305 9 0.04390273291168967 2305 10-11 0.10315342563462337 2305 12-13 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-0.29430659120116687 1213 82-83 -0.23584844345419853 1213 84-85 -0.25438884815494234 1213 86-87 -0.23145504540910267 1213 88-89 -0.15980090320854146 1213 90-91 -0.10631480998504017 1213 92-93 -0.036712992456919835 1213 94-95 -0.02294013935730277 1213 96-97 -0.0022642287553686913 1213 98-99 0.009647380642995529 1213 100-101 -0.09065855039148474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 324.0 3 180.0 4 160.0 5 161.0 6 415.0 7 2922.0 8 17267.0 9 46366.0 10 49294.0 11 32548.0 12 19202.0 13 12401.0 14 9268.0 15 7494.0 16 6862.0 17 6618.0 18 6871.0 19 7251.0 20 8027.0 21 8857.0 22 10030.0 23 11113.0 24 12443.0 25 14561.0 26 17266.0 27 21178.0 28 26190.0 29 32052.0 30 39236.0 31 51680.0 32 70884.0 33 101699.0 34 154337.0 35 260608.0 36 483955.0 37 667645.0 38 130148.0 39 89.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.31212276948452 23.451466066114264 22.20996682505724 14.02644433934398 2 32.37965658418502 25.173754335589454 29.89210479931134 12.554484280914188 3 36.5064654786195 20.04106366147568 27.821057329547834 15.631413530356985 4 30.070160379990597 17.591629558654265 32.46545094020711 19.872759121148025 5 34.29218000945478 16.02158557002596 31.93279426296235 17.753440157556913 6 31.3749131030012 16.412635996468676 36.05933305753754 16.153117842992586 7 36.2900037909494 18.296085514266377 27.843777064884083 17.57013362990014 8 29.651694070019374 29.894706140457476 23.14371228121345 17.3098875083097 9 31.590829438505203 26.81952641277589 23.45880350538795 18.130840643330952 10-11 26.712868514015835 29.1083361771634 27.582728810730515 16.59606649809025 12-13 28.507841113042765 24.470233960545624 27.390060887648875 19.631864038762732 14-15 26.010981970678497 24.599315838790385 29.030501565426515 20.359200625104602 16-17 27.077977172629325 25.421329990662695 29.823038845755363 17.677653990952617 18-19 26.629704444334546 25.57743435649153 29.81149820852099 17.98136299065293 20-21 25.271102206954975 27.695412201768388 28.890374493998326 18.143111097278307 22-23 27.844930414104923 25.494941029119246 28.344923166906394 18.31520538986944 24-25 24.7151312382592 28.231609303781475 28.53639439768436 18.516865060274966 26-27 22.719260305137787 29.887294773702955 28.891812670210626 18.501632250948635 28-29 23.535403059397332 29.11174730128413 28.275934105288293 19.076915534030253 30-31 25.686851488266228 27.252421242032042 28.068828406224643 18.991898863477086 32-33 23.135866284165356 26.861626245172587 29.61960923150399 20.38289823915807 34-35 20.66150693081646 26.888571728551348 33.61285933417385 18.83706200645835 36-37 21.536145510389925 27.604321280241486 30.35995800877599 20.499575200592606 38-39 20.34946491852399 27.208602279044804 28.15705540326117 24.284877399170036 40-41 22.069746433835682 26.487814444856788 29.321454050509217 22.12098507079831 42-43 24.16181556079097 26.5581985432382 30.401439772081623 18.878546123889212 44-45 22.07371363075408 28.430138376043185 30.865776966661752 18.630371026540978 46-47 23.52931634364734 29.013768492867946 28.989777629886298 18.467137533598414 48-49 20.89591514427181 28.843413863752776 30.4729597063343 19.787711285641112 50-51 19.82242149518608 27.597467664444835 31.022732584110436 21.557378256258648 52-53 24.34100129773431 26.89293467857227 28.269501920565688 20.49656210312773 54-55 26.160513923819973 26.81130429271996 28.166036466878293 18.862145316581778 56-57 26.662043241120887 26.76165316626407 27.234053449644495 19.342250142970542 58-59 24.92667808237447 26.701873989789878 27.66162561812857 20.709822309707086 60-61 21.312708232752616 27.0486625422439 27.45443440160278 24.184194823400706 62-63 23.573599517394204 27.109757835304798 27.671802459275867 21.64484018802513 64-65 21.629418958796386 28.34006482110199 29.645702524771895 20.38481369532973 66-67 20.816017480877093 28.199029736223004 28.58427211762128 22.400680665278625 68-69 20.830422189306887 26.898437464999603 32.74995962621017 19.521180719483336 70-71 20.294305431800062 30.97547390738863 29.944709471406846 18.78551118940446 72-73 20.082885916348665 34.17750223566274 26.958764821932025 18.780847026056563 74-75 20.772070037785046 33.64221481718172 26.523570966306593 19.06214417872664 76-77 20.789139486904457 33.03484287331627 26.708368292684487 19.467649347094785 78-79 20.692257002982924 32.75466056670665 27.24797186497972 19.305110565330704 80-81 19.75996767540233 32.805242592350815 27.465817249968637 19.968972482278218 82-83 19.154452433628318 32.4246916994428 27.853615276412103 20.567240590516768 84-85 19.37356060261739 31.701491734740973 28.141911218854975 20.783036443786656 86-87 19.363699915156005 31.673419759261566 28.886136627852387 20.07674369773004 88-89 19.75559985846969 31.84666752882357 28.706794521974544 19.690938090732196 90-91 20.488985785922136 31.12564708854429 27.912277175585192 20.47308994994839 92-93 19.991492464495224 31.744968490332703 27.597139607846987 20.666399437325083 94-95 19.72420228323185 31.890686979127338 27.793720664413048 20.59139007322777 96-97 20.254895920095034 32.369716639130495 27.226504362548987 20.148883078225488 98-99 20.70506840464276 31.454357216563917 27.145150648967125 20.6954237298262 100-101 20.743518270736477 31.486669492093288 26.74870220302184 21.021110034148396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 127.0 2 152.5 3 143.5 4 116.5 5 126.0 6 158.0 7 183.5 8 256.0 9 352.0 10 436.0 11 500.5 12 546.5 13 548.0 14 487.0 15 473.5 16 463.0 17 418.5 18 417.5 19 424.0 20 457.5 21 541.0 22 649.5 23 791.0 24 993.5 25 1236.5 26 1717.0 27 2477.0 28 3332.5 29 4266.0 30 5397.0 31 6913.0 32 10996.0 33 19451.0 34 33814.0 35 53897.5 36 81954.5 37 117126.0 38 146341.5 39 161815.5 40 165216.5 41 153418.0 42 143175.5 43 145609.0 44 141785.5 45 132528.0 46 127715.0 47 115173.5 48 93825.0 49 78879.0 50 68918.5 51 58920.0 52 49222.5 53 42219.5 54 35459.5 55 27494.0 56 21801.0 57 17790.0 58 14533.0 59 11725.0 60 9187.0 61 7286.0 62 5783.5 63 4473.5 64 3570.0 65 2745.5 66 2030.0 67 1602.0 68 1291.0 69 992.0 70 694.0 71 508.0 72 384.0 73 269.0 74 191.0 75 146.0 76 110.5 77 70.0 78 55.0 79 45.5 80 33.0 81 26.5 82 23.0 83 18.0 84 16.5 85 18.5 86 15.0 87 11.0 88 9.5 89 8.5 90 8.0 91 4.5 92 4.0 93 4.5 94 2.5 95 3.0 96 2.5 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2074457254679456 2 0.19147197864033172 3 0.16714928680415164 4 0.15096255668550293 5 0.1624636544013849 6 0.12233760237041884 7 0.10832330182032558 8 0.10440440926528431 9 0.09303110152402323 10-11 0.08010301575820773 12-13 0.10491556916376796 14-15 0.10199769807659051 16-17 0.0925199416255396 18-19 0.08749353595711709 20-21 0.09075218030995033 22-23 0.0795705575306206 24-25 0.3863090932790141 26-27 0.18595571140252903 28-29 0.08698237605863345 30-31 0.1669150051840133 32-33 0.196093716055788 34-35 0.19543346785357996 36-37 0.20154608830628018 38-39 0.20167387828090108 40-41 0.19779758238406678 42-43 0.21336666095871448 44-45 0.19605111939758102 46-47 0.21483624566685494 48-49 0.20454915270987162 50-51 0.20716884718960027 52-53 0.19903288547206896 54-55 0.18305913864445508 56-57 0.1900662889195017 58-59 0.1848481982891478 60-61 0.16700019850042724 62-63 0.15590376903751146 64-65 0.16044031313655382 66-67 0.17091909105546851 68-69 0.1652111388557345 70-71 0.15805490027696348 72-73 0.160930174705934 74-75 0.15134592660936563 76-77 0.16222937278124655 78-79 0.16022732984551896 80-81 0.16510464721021706 82-83 0.18887358248970654 84-85 0.18702062785770332 86-87 0.1907478354508132 88-89 0.19769109073854937 90-91 0.17980049429162184 92-93 0.1617608095409699 94-95 0.16267663769241975 96-97 0.17115337267560687 98-99 0.1848694966182513 100-101 0.19564645114461482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2347602.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.12622208433054 #Duplication Level Percentage of deduplicated Percentage of total 1 88.32044573663677 38.08927157425263 2 4.8510570414289385 4.184155266248397 3 1.8337771503338727 2.3725164191540817 4 0.9376001720831839 1.617406129902637 5 0.7061818589182789 1.522747783981754 6 0.45909262567882664 1.1879358319382105 7 0.30747649263959764 0.9282209655100422 8 0.2695785634301303 0.930072399653007 9 0.21185561194733216 0.8222878953587158 >10 1.614680249452655 14.450307036785237 >50 0.24622246810976595 7.486541115147577 >100 0.22386264324379804 19.283379979334256 >500 0.013740392481977427 3.82705167969773 >1k 0.004328971426332963 3.0389892254993067 >5k 1.000221885469745E-4 0.25911669753642097 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 6007 0.25587812584927083 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3621 0.1542424993674396 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3330 0.14184687182921127 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3224 0.1373316260592724 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2743 0.11684263346171965 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2678 0.11407385067826659 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2669 0.11369048075440386 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2658 0.11322191751412718 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2507 0.10678982212487466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004728229060973708 0.0 0.0 2 0.005324582275871293 0.0 0.0 3 0.005750548857940997 0.0 0.0 4 0.0061765154400107 0.0 0.0 5 0.007071045262357078 0.0 0.0 6 0.008434138324980129 0.0 0.0 7 0.010521374577121675 0.0 0.0 8 0.011628887690502904 0.0 0.0 9 0.013332754018781718 0.0 0.0 10-11 0.021426119078106085 0.0 0.0 12-13 0.028411971024049222 0.0 0.0 14-15 0.03535522631178539 0.0 0.0 16-17 0.04104188018241593 0.0 0.0 18-19 0.04664334073663253 0.0 0.0 20-21 0.052905049493057174 0.0 0.0 22-23 0.05984830478079334 0.0 0.0 24-25 0.06738791328342708 0.0 0.0 26-27 0.07467194183681902 0.0 0.0 28-29 0.08293569352897126 0.0 0.0 30-31 0.13846043750175713 0.0 0.0 32-33 0.14900311040798225 0.0 0.0 34-35 0.15854476184634364 2.1298329103485175E-5 0.0 36-37 0.16976898128388032 4.259665820697035E-5 0.0 38-39 0.18239889044224702 4.259665820697035E-5 0.0 40-41 0.19730772081468664 4.259665820697035E-5 0.0 42-43 0.2147297540213375 4.259665820697035E-5 0.0 44-45 0.23502706165695889 8.51933164139407E-5 0.0 46-47 0.25615500412761616 8.51933164139407E-5 0.0 48-49 0.2787951279646209 8.51933164139407E-5 0.0 50-51 0.30558842597680524 8.51933164139407E-5 0.0 52-53 0.3345754518866486 8.51933164139407E-5 0.0 54-55 0.3646273942516661 8.51933164139407E-5 0.0 56-57 0.3994927589940714 8.51933164139407E-5 0.0 58-59 0.4358703051028241 8.51933164139407E-5 0.0 60-61 0.4752296172860647 8.51933164139407E-5 0.0 62-63 0.5154834592916517 8.51933164139407E-5 0.0 64-65 0.5588894540045544 8.51933164139407E-5 0.0 66-67 0.6074496443605006 8.51933164139407E-5 0.0 68-69 0.6560950280328608 8.51933164139407E-5 0.0 70-71 0.7090639725132284 8.51933164139407E-5 0.0 72-73 0.7678473608388474 8.51933164139407E-5 0.0 74-75 0.8281216322017104 8.51933164139407E-5 0.0 76-77 0.8920805144994766 8.51933164139407E-5 0.0 78-79 0.960320360947043 8.51933164139407E-5 0.0 80-81 1.0361637108845536 8.51933164139407E-5 0.0 82-83 1.1186095428441447 8.51933164139407E-5 0.0 84-85 1.2088079665974045 8.51933164139407E-5 0.0 86-87 1.3052680991070889 8.51933164139407E-5 0.0 88-89 1.4082881169806467 8.51933164139407E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 2435 0.0 85.59318 4 GAGCGGC 1785 0.0 82.915924 9 AGAGCGG 1885 0.0 82.79539 8 ATTCGGA 265 0.0 82.43042 2 AAGCGGC 715 0.0 80.27881 9 CGGAAAG 1105 0.0 78.19567 5 TCGGGAG 2435 0.0 77.60259 3 GAGAGCG 2280 0.0 76.356064 7 ACCGGAA 415 0.0 74.36308 3 TCGGGAA 1045 0.0 71.330536 3 TCGGAGA 545 0.0 70.56058 4 AACCGGA 360 0.0 69.911545 2 ATCGGGA 3540 0.0 69.48675 2 GATCCGG 660 0.0 69.07655 1 GTTCGGA 290 0.0 68.77441 2 GGATCGG 740 0.0 68.0264 1 AATCGGA 460 0.0 66.068985 2 GAACCGG 405 0.0 65.66536 1 GATCGGG 3925 0.0 65.45773 1 ATCCGGA 680 0.0 64.24724 2 >>END_MODULE