FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347602
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT60070.25587812584927083Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36210.1542424993674396No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33300.14184687182921127No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32240.1373316260592724No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27430.11684263346171965No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT26780.11407385067826659No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC26690.11369048075440386No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26580.11322191751412718No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT25070.10678982212487466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA24350.085.593184
GAGCGGC17850.082.9159249
AGAGCGG18850.082.795398
ATTCGGA2650.082.430422
AAGCGGC7150.080.278819
CGGAAAG11050.078.195675
TCGGGAG24350.077.602593
GAGAGCG22800.076.3560647
ACCGGAA4150.074.363083
TCGGGAA10450.071.3305363
TCGGAGA5450.070.560584
AACCGGA3600.069.9115452
ATCGGGA35400.069.486752
GATCCGG6600.069.076551
GTTCGGA2900.068.774412
GGATCGG7400.068.02641
AATCGGA4600.066.0689852
GAACCGG4050.065.665361
GATCGGG39250.065.457731
ATCCGGA6800.064.247242