##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C68Y0ACXX_l07n02_34042-1.34200000035847.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5469189
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.985313727501463	33.0	33.0	33.0	27.0	33.0
2	30.445807413128346	33.0	33.0	33.0	22.0	33.0
3	30.288444593887686	33.0	33.0	33.0	22.0	33.0
4	33.601734370488934	37.0	37.0	37.0	15.0	37.0
5	33.65633295905481	37.0	37.0	37.0	22.0	37.0
6	34.36080998480762	37.0	37.0	37.0	27.0	37.0
7	33.64705187551573	37.0	37.0	37.0	22.0	37.0
8	34.02777889006944	37.0	37.0	37.0	27.0	37.0
9	33.358952122517614	37.0	37.0	37.0	15.0	37.0
10-11	33.43264622963295	37.0	37.0	37.0	15.0	37.0
12-13	33.42307058688226	37.0	37.0	37.0	15.0	37.0
14-15	34.98779078580023	40.0	37.0	40.0	15.0	40.0
16-17	34.89603184311238	40.0	37.0	40.0	15.0	40.0
18-19	34.9572366213711	40.0	37.0	40.0	15.0	40.0
20-21	34.907118678838856	40.0	37.0	40.0	15.0	40.0
22-23	35.83884804492952	40.0	37.0	40.0	24.5	40.0
24-25	35.10022116624604	40.0	37.0	40.0	22.0	40.0
26-27	34.937117642122075	40.0	37.0	40.0	18.5	40.0
28-29	34.61941743099388	40.0	37.0	40.0	15.0	40.0
30-31	34.563824453680425	40.0	37.0	40.0	15.0	40.0
32-33	34.36553573116599	38.5	37.0	40.0	15.0	40.0
34-35	34.6732465270445	37.0	37.0	40.0	18.5	40.0
36-37	34.38851765773683	37.0	37.0	40.0	15.0	40.0
38-39	34.31346009435768	37.0	37.0	40.0	15.0	40.0
40-41	34.10251977029867	37.0	37.0	40.0	15.0	40.0
42-43	34.018178563585934	37.0	37.0	40.0	15.0	40.0
44-45	33.86898057463364	37.0	33.0	40.0	15.0	40.0
46-47	33.79819466835028	37.0	33.0	40.0	15.0	40.0
48-49	33.68928647373495	37.0	33.0	40.0	15.0	40.0
50-51	32.75202959707555	37.0	33.0	38.5	15.0	38.5
52-53	33.17769682122889	37.0	33.0	38.5	15.0	40.0
54-55	33.79595841357832	37.0	33.0	40.0	15.0	40.0
56-57	34.13477884929557	37.0	33.0	40.0	18.5	40.0
58-59	33.49928773717639	37.0	33.0	40.0	15.0	40.0
60-61	33.314490960177096	37.0	33.0	40.0	15.0	40.0
62-63	32.897163180866485	37.0	33.0	40.0	6.0	40.0
64-65	32.67616304720865	37.0	33.0	37.0	10.5	40.0
66-67	32.52632236699079	37.0	33.0	37.0	15.0	40.0
68-69	32.66931870520474	37.0	33.0	37.0	15.0	40.0
70-71	32.219208186076585	37.0	33.0	37.0	15.0	38.5
72-73	32.586475892495216	37.0	33.0	37.0	18.5	37.0
74-75	32.70551685085302	37.0	33.0	37.0	22.0	37.0
76-77	32.43129100128008	37.0	33.0	37.0	22.0	37.0
78-79	31.96279146688842	37.0	33.0	37.0	22.0	37.0
80-81	31.414076108907555	35.0	33.0	37.0	15.0	37.0
82-83	31.078277419193228	33.0	33.0	37.0	15.0	37.0
84-85	30.825023966076138	33.0	33.0	37.0	15.0	37.0
86-87	30.65495542392117	33.0	33.0	37.0	15.0	37.0
88-89	30.49984668659284	33.0	30.0	37.0	15.0	37.0
90-91	30.369716332714045	33.0	27.0	37.0	15.0	37.0
92-93	30.182268065704072	33.0	27.0	37.0	15.0	37.0
94-95	30.06358968395497	33.0	27.0	37.0	10.5	37.0
96-97	29.930924859243298	33.0	27.0	37.0	6.0	37.0
98-99	29.787772556406445	33.0	27.0	37.0	6.0	37.0
100-101	28.58131443254201	33.0	24.5	35.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.07608343409505025
2306	2	0.13028849079351446
2306	3	0.1466455402547453
2306	4	0.39309188228256176
2306	5	0.3270874184936048
2306	6	-0.033895022906101246
2306	7	0.5571764044898941
2306	8	-0.06935006827969659
2306	9	0.33519967089137026
2306	10-11	0.27621860939321863
2306	12-13	0.27086785535304614
2306	14-15	0.6362580194371787
2306	16-17	0.5407679656183007
2306	18-19	0.6009498857167301
2306	20-21	0.47935319735907456
2306	22-23	0.3585925467624804
2306	24-25	0.5896104615227529
2306	26-27	0.19374922672976425
2306	28-29	0.44946160513320876
2306	30-31	0.45899445276831585
2306	32-33	0.5706777295876364
2306	34-35	0.3275989480585153
2306	36-37	0.7085073452945565
2306	38-39	0.46274602037174617
2306	40-41	0.4899714546667795
2306	42-43	0.5530270093030154
2306	44-45	0.6578350516578837
2306	46-47	0.6539014388192967
2306	48-49	0.6019740596966372
2306	50-51	0.5570457963690458
2306	52-53	0.49952926813934795
2306	54-55	0.48791797446027374
2306	56-57	0.36540357907595933
2306	58-59	0.7701795771502731
2306	60-61	0.7006021343377569
2306	62-63	0.7070618985435786
2306	64-65	0.6582442029668627
2306	66-67	0.5581152995413206
2306	68-69	0.3678184722393638
2306	70-71	0.5055315322867457
2306	72-73	0.02690651717397685
2306	74-75	0.063579470223452
2306	76-77	0.40406615630622866
2306	78-79	0.32438193593596765
2306	80-81	0.6167628232792559
2306	82-83	0.8001765773118272
2306	84-85	0.7743220246708944
2306	86-87	0.675195357287425
2306	88-89	0.6363569528881001
2306	90-91	0.6357536106413981
2306	92-93	0.6652618713431693
2306	94-95	0.5472572296562355
2306	96-97	0.5734664776265355
2306	98-99	0.6056077325195552
2306	100-101	0.5328721835827146
2307	1	0.06708447141906504
2307	2	0.08200301000208654
2307	3	0.13008490736919143
2307	4	0.26950123722109254
2307	5	0.24191857101848768
2307	6	-0.04909264505238298
2307	7	0.5281661803372515
2307	8	1.1068306359618418E-4
2307	9	0.3106803470306332
2307	10-11	0.20495007400616316
2307	12-13	0.15939407313449294
2307	14-15	0.5242546360158613
2307	16-17	0.42358012376842424
2307	18-19	0.45348175939010815
2307	20-21	0.4012148140386387
2307	22-23	0.13574074941980285
2307	24-25	0.3961653523128916
2307	26-27	0.1309280761673861
2307	28-29	0.3795127167258556
2307	30-31	0.4112644145741555
2307	32-33	0.39856591973627786
2307	34-35	0.1447069534277574
2307	36-37	0.3258980535544467
2307	38-39	0.26434437448780557
2307	40-41	0.323708348265221
2307	42-43	0.2918239296114251
2307	44-45	0.37319422018465076
2307	46-47	0.30978445990299264
2307	48-49	0.31501952273381306
2307	50-51	0.07459131718090362
2307	52-53	0.15847919439475078
2307	54-55	0.31186173284317675
2307	56-57	0.18723721892513367
2307	58-59	0.4640186939339799
2307	60-61	0.2517247644296887
2307	62-63	0.33335462330999377
2307	64-65	0.43032998146163237
2307	66-67	0.3713161777688043
2307	68-69	0.1896337381692561
2307	70-71	0.21351512794681327
2307	72-73	-0.09199147475643343
2307	74-75	-0.13069413571029287
2307	76-77	0.11306549235597885
2307	78-79	0.2330616247440105
2307	80-81	0.461939757899021
2307	82-83	0.5364415977451955
2307	84-85	0.5017535132059621
2307	86-87	0.4952159400072702
2307	88-89	0.4096036290100713
2307	90-91	0.4176685188923308
2307	92-93	0.6348742910383329
2307	94-95	0.5056119391832397
2307	96-97	0.4251787958950466
2307	98-99	0.40709538459075745
2307	100-101	0.3445833332568604
2201	1	-0.1137764870300586
2201	2	-0.15099905498000155
2201	3	-0.1397756921352027
2201	4	-0.040775229270686
2201	5	-0.08952062688209139
2201	6	-0.26390426577057724
2201	7	0.18406294062022965
2201	8	-0.2632488913608668
2201	9	-0.007201955660313786
2201	10-11	-0.014646529433036903
2201	12-13	-0.024346317435899323
2201	14-15	0.2600966072029536
2201	16-17	0.11361989548648666
2201	18-19	0.2439364420624628
2201	20-21	0.18712302819103854
2201	22-23	0.038190602766299264
2201	24-25	0.23912292717065498
2201	26-27	-0.12225619958319811
2201	28-29	0.045442760300701934
2201	30-31	0.16298394633037816
2201	32-33	0.14881190680587508
2201	34-35	-0.04661765760634751
2201	36-37	0.31514036520788835
2201	38-39	0.0076323180704847005
2201	40-41	0.024753184982422738
2201	42-43	0.13696907865748642
2201	44-45	0.21915635139240663
2201	46-47	0.23445646273304277
2201	48-49	0.22472710629963188
2201	50-51	0.11906634137199745
2201	52-53	0.08125185342913
2201	54-55	0.1958201582492478
2201	56-57	0.32517822007212516
2201	58-59	0.4117782476567413
2201	60-61	0.30343655157670213
2201	62-63	0.3329297505896207
2201	64-65	0.2943853478829297
2201	66-67	0.25371907309158104
2201	68-69	0.17111396273574542
2201	70-71	0.33701335928557796
2201	72-73	0.05552251803552366
2201	74-75	0.16100289591810935
2201	76-77	0.32282239685419967
2201	78-79	0.45500455450867605
2201	80-81	0.6031770660655447
2201	82-83	0.5233448089047457
2201	84-85	0.5140756867262191
2201	86-87	0.37881640883404444
2201	88-89	0.41755880637397524
2201	90-91	0.4161374145901071
2201	92-93	0.4797920734269212
2201	94-95	0.38331322676466684
2201	96-97	0.32348096016129446
2201	98-99	0.2729901265816359
2201	100-101	0.23734816368944323
1101	1	-0.019941136162429984
1101	2	-0.04950770744370203
1101	3	-0.008307211052013486
1101	4	0.09074648697590959
1101	5	0.049743421077280914
1101	6	-0.003334432680603072
1101	7	0.31856519499745417
1101	8	0.038653342146801606
1101	9	0.26768859531043177
1101	10-11	0.13702076662853813
1101	12-13	0.1265122688022231
1101	14-15	0.3570659775370899
1101	16-17	0.24450864105092052
1101	18-19	0.22917667749727144
1101	20-21	0.26142130354957516
1101	22-23	0.1583067139353247
1101	24-25	0.24537995193195172
1101	26-27	0.10272019022027479
1101	28-29	0.2874199831082791
1101	30-31	0.28270673110079514
1101	32-33	0.1493389808160046
1101	34-35	0.08358132545288299
1101	36-37	0.301260730486014
1101	38-39	-0.0055013646606738575
1101	40-41	0.18575760172265632
1101	42-43	0.11891960471899665
1101	44-45	0.1935625426878076
1101	46-47	0.1728383960035771
1101	48-49	0.1982701766158783
1101	50-51	0.27430320429036215
1101	52-53	0.2156098623124052
1101	54-55	0.2837635295736334
1101	56-57	0.3494605105402684
1101	58-59	0.4293912850400474
1101	60-61	0.25558926857495834
1101	62-63	0.4272992314211521
1101	64-65	0.40556288503038473
1101	66-67	0.32052642094384964
1101	68-69	0.19356643787541827
1101	70-71	0.29724064016135543
1101	72-73	0.10261960400118397
1101	74-75	0.18942321644541238
1101	76-77	0.32098690234341376
1101	78-79	0.48760025002120955
1101	80-81	0.5923523724990183
1101	82-83	0.4560787380091611
1101	84-85	0.40586417424351495
1101	86-87	0.2862968212482322
1101	88-89	0.2836485350450175
1101	90-91	0.3325137784758532
1101	92-93	0.3783368948243826
1101	94-95	0.4025630159608795
1101	96-97	0.25111713476276165
1101	98-99	0.22799757921768204
1101	100-101	0.16814236520989212
2304	1	0.0833859679117559
2304	2	-0.0727831761288904
2304	3	0.013576940461369702
2304	4	0.14012794110453086
2304	5	0.14078934136182397
2304	6	0.09302108622395622
2304	7	0.44590797849085106
2304	8	0.05645720916837149
2304	9	0.14989738330162794
2304	10-11	0.14456775367348484
2304	12-13	0.13294298722252051
2304	14-15	0.3727502217000662
2304	16-17	0.27591766947162455
2304	18-19	0.34657355448346294
2304	20-21	0.24888119809393316
2304	22-23	0.20381438631870452
2304	24-25	0.3756010559305025
2304	26-27	0.26646417653251575
2304	28-29	0.33753420076075713
2304	30-31	0.35792051192195373
2304	32-33	0.2868494350994908
2304	34-35	0.29595079539455327
2304	36-37	0.46263380304182533
2304	38-39	0.27086856407110105
2304	40-41	0.29552890261863496
2304	42-43	0.3091864891308944
2304	44-45	0.3889552054970622
2304	46-47	0.2900234208387076
2304	48-49	0.35203623866173217
2304	50-51	0.17407448053712216
2304	52-53	0.1272992708533991
2304	54-55	0.33823953101754967
2304	56-57	0.3963957921337098
2304	58-59	0.523313978698063
2304	60-61	0.38769703411140455
2304	62-63	0.5170436468395323
2304	64-65	0.3512892693893903
2304	66-67	0.28911616006230645
2304	68-69	0.26205001229294567
2304	70-71	0.4363934140662096
2304	72-73	0.2812171820950198
2304	74-75	0.39687941570506524
2304	76-77	0.4616243719953772
2304	78-79	0.5239618567578503
2304	80-81	0.48966609561479046
2304	82-83	0.5584913823650695
2304	84-85	0.4544426932482999
2304	86-87	0.4032576431013659
2304	88-89	0.4795915833968678
2304	90-91	0.5247356080005083
2304	92-93	0.4449668481434834
2304	94-95	0.5087793753523329
2304	96-97	0.4705178200004987
2304	98-99	0.48547464377707783
2304	100-101	0.40903284071583457
2202	1	0.08774981200234677
2202	2	0.04439969249906639
2202	3	0.0653404987935069
2202	4	0.08858133884852037
2202	5	0.13571246397805936
2202	6	0.184036861948087
2202	7	0.3114736349130496
2202	8	0.20078751377192816
2202	9	0.15823644418929916
2202	10-11	0.19791042419954863
2202	12-13	0.13578538917160188
2202	14-15	0.23989852894295893
2202	16-17	0.23817216588271606
2202	18-19	0.2516433880871958
2202	20-21	0.2635420156974746
2202	22-23	0.3215244146960927
2202	24-25	0.22991065143821743
2202	26-27	0.21044883859389785
2202	28-29	0.338985348559774
2202	30-31	0.3539531135772549
2202	32-33	0.32716500983440966
2202	34-35	0.1523195130349464
2202	36-37	0.19695081245079393
2202	38-39	0.21873206316949023
2202	40-41	0.2380996920102305
2202	42-43	0.28224713875486884
2202	44-45	0.32624581383338835
2202	46-47	0.3034977942710455
2202	48-49	0.3098833037079558
2202	50-51	0.24881577703354196
2202	52-53	0.18902576111378266
2202	54-55	0.3377215764796304
2202	56-57	0.37495183348534056
2202	58-59	0.44764431868788535
2202	60-61	0.38174238601276755
2202	62-63	0.38229553705201624
2202	64-65	0.38614089357364634
2202	66-67	0.4793818808062227
2202	68-69	0.46537295497479647
2202	70-71	0.43499735407844753
2202	72-73	0.38910474768095327
2202	74-75	0.4584822931250798
2202	76-77	0.48301945933896917
2202	78-79	0.4412718959806625
2202	80-81	0.5839064449786271
2202	82-83	0.6238340861733533
2202	84-85	0.5159915109715776
2202	86-87	0.579934750151569
2202	88-89	0.6191420400781737
2202	90-91	0.513934547541016
2202	92-93	0.5906124698794919
2202	94-95	0.5731054651644953
2202	96-97	0.4580408133038425
2202	98-99	0.5010336509283064
2202	100-101	0.5189201129783036
1102	1	0.013858132083282015
1102	2	-0.14403413881946037
1102	3	-0.0901496226866989
1102	4	-0.03758319225404705
1102	5	0.011204086501066968
1102	6	0.10765312026267537
1102	7	-0.02596487595694441
1102	8	0.06369283724229291
1102	9	-0.06395973484836759
1102	10-11	0.019381247725917206
1102	12-13	0.034757055426950956
1102	14-15	-0.12474406325691945
1102	16-17	-0.03025075761311058
1102	18-19	-0.022948118591877176
1102	20-21	-0.06928705819723291
1102	22-23	0.051154269390281115
1102	24-25	0.06672784633369133
1102	26-27	0.036990205850059965
1102	28-29	0.041291424324498394
1102	30-31	0.07961441410774484
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1213	78-79	-0.2333644225400917
1213	80-81	-0.5294051146902667
1213	82-83	-0.387752478618026
1213	84-85	-0.42888599127971716
1213	86-87	-0.3768753845343902
1213	88-89	-0.35441746818522546
1213	90-91	-0.39259312719386585
1213	92-93	-0.3580281236152878
1213	94-95	-0.33634717652220303
1213	96-97	-0.3210309258043189
1213	98-99	-0.4341867190205555
1213	100-101	-0.203636187817807
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	760.0
3	497.0
4	454.0
5	453.0
6	1027.0
7	6745.0
8	35487.0
9	118312.0
10	151015.0
11	101852.0
12	59564.0
13	37466.0
14	25996.0
15	19940.0
16	17185.0
17	15940.0
18	15759.0
19	16414.0
20	17379.0
21	19005.0
22	20939.0
23	22100.0
24	24091.0
25	27686.0
26	33011.0
27	40536.0
28	50658.0
29	64200.0
30	77077.0
31	98917.0
32	137801.0
33	200262.0
34	308501.0
35	531116.0
36	1069314.0
37	1735902.0
38	365768.0
39	60.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.55470120084075	23.865395152290354	22.45152565232609	14.128377994542799
2	30.803603001450742	26.856549430970993	29.61209946547148	12.727748102106784
3	34.55686116479068	20.28202991198586	29.13022612582084	16.030882797402622
4	27.425653178809643	17.778294647468417	33.59696129459985	21.19909087912209
5	34.07900899268947	16.686835821371197	30.961139133832035	18.2730160521073
6	31.28955442714808	16.928809629391747	35.57365385411124	16.207982089348928
7	35.49134643160897	19.496661114977805	27.662037248915443	17.349955204497782
8	27.240261469781768	31.719292503438247	23.870750791122006	17.16969523565798
9	32.2282492510345	26.904773758240086	23.09994845828782	17.7670285324376
10-11	26.85524592648009	29.833587589004733	26.518021651749272	16.793144832765893
12-13	29.146122766676058	23.926055414688474	26.218298385249234	20.709523433386234
14-15	25.791047180340936	24.20137002788289	28.283255026631295	21.724327765144878
16-17	27.553856765534672	25.23870931371513	29.33655215117279	17.870881769577412
18-19	27.116113437236482	25.44380138554815	29.606448870564584	17.83363630665079
20-21	25.516665287867617	28.283887525138677	28.31579818188873	17.883649005104974
22-23	29.032180731163066	25.686701210845797	27.32559921682211	17.95551884116903
24-25	24.847917632962897	29.627874713117304	27.262014376193118	18.262193277726684
26-27	22.809202888096564	31.755120921296392	26.871224143498008	18.56445204710904
28-29	24.190943005137726	29.984359059552425	26.786242890180063	19.038455045129783
30-31	26.978463155937778	26.756633616305965	27.383337324775315	18.88156590298094
32-33	23.084084906102873	29.069953432536543	29.29183413071137	18.554127530649218
34-35	27.847587205699178	26.633918504952764	27.780519463982078	17.737974825365992
36-37	24.211676706256096	27.05386491440857	29.62885904878177	19.105599330553563
38-39	20.590756064090083	29.73570156622889	31.076739874371505	18.59680249530952
40-41	21.93543374995431	30.83336318380522	28.20344652479585	19.027756541444624
42-43	25.001928756057712	27.150141518467034	29.679038499070444	18.168891226404806
44-45	22.363568222004005	29.285253157199122	30.08436328844322	18.26681533235365
46-47	24.21385307469413	30.176210969369244	27.287438508683003	18.322497447253628
48-49	21.180360070564706	29.3620270815649	29.538374712145565	19.919238135724825
50-51	19.859410592059465	27.01451228359249	31.47841793258459	21.647659191763456
52-53	25.74118980093151	26.511350168097536	27.577945229437084	20.16951480153387
54-55	27.59366454205418	26.5289217969486	27.166268902936945	18.711144758060268
56-57	28.192849599832325	26.087645551327064	26.726087224167916	18.993417624672695
58-59	26.224875171636135	25.931992243283176	27.262734508321433	20.580398076759256
60-61	21.836169021431637	26.50427986172712	26.463709413754277	25.195841703086963
62-63	24.73336661014005	26.638257760459922	26.8557016383562	21.77267399104383
64-65	22.136391175410473	28.157509062244507	29.738548385206197	19.967551377138822
66-67	20.658353884491788	28.077377801928016	28.26107205014669	23.003196263433505
68-69	20.028913546828235	26.416909864224834	33.91086857954097	19.64330800940597
70-71	19.571246472153593	31.062319029034107	30.622979672645158	18.74345482616714
72-73	19.43553331474336	35.674351285478345	26.426169187989046	18.46394621178925
74-75	20.347491652056373	35.405078659344106	25.658978662393288	18.58845102620623
76-77	20.801640402503388	34.39091467128533	26.1074259972221	18.700018928989184
78-79	20.41776782009579	34.11594095928119	26.88021938483638	18.586071835786647
80-81	19.68137833113297	34.46894740081147	26.600599592944967	19.24907467511059
82-83	18.945024234669575	34.12522882599258	27.035113156193802	19.894633783144045
84-85	19.221016077535648	33.04024032716592	27.453137530875622	20.28560606442281
86-87	19.17123421252694	32.810498840773306	28.049227904362358	19.9690390423374
88-89	19.627285116506265	33.118647415505606	27.594315164045412	19.659752303942714
90-91	20.233381277014118	32.01396158576312	27.383807407275786	20.368849729946984
92-93	19.95291316050515	32.50514843462678	26.827795026733032	20.714143378135034
94-95	19.584170542148666	32.975744701076046	26.71870525582302	20.72137950095227
96-97	20.32433132445762	33.09208385495321	26.501873710184288	20.081711110404882
98-99	20.802070348277127	31.900184635305195	26.524793864212583	20.772951152205092
100-101	20.76170819339933	31.873622417290072	26.088709455782688	21.275959933527915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184.0
1	251.0
2	327.0
3	325.0
4	291.0
5	282.5
6	321.0
7	428.0
8	594.0
9	767.0
10	997.0
11	1188.0
12	1297.0
13	1302.5
14	1198.0
15	1137.5
16	1050.5
17	987.0
18	960.0
19	956.5
20	1081.5
21	1256.0
22	1443.0
23	1688.5
24	2063.0
25	2563.5
26	3186.0
27	4026.0
28	4923.0
29	6031.0
30	7980.0
31	11662.5
32	21144.0
33	39972.5
34	71134.5
35	114283.0
36	172113.0
37	251938.0
38	328198.5
39	355837.0
40	358987.5
41	342071.0
42	322950.5
43	348381.0
44	357809.0
45	344991.0
46	356235.5
47	333748.5
48	265863.0
49	214819.0
50	179559.0
51	150130.0
52	120011.5
53	89127.5
54	67484.5
55	51432.0
56	39658.5
57	29391.5
58	20460.5
59	14804.5
60	11080.5
61	8427.5
62	6287.0
63	4491.5
64	3329.0
65	2508.5
66	1885.0
67	1429.5
68	1113.0
69	821.0
70	608.0
71	425.5
72	306.0
73	250.5
74	190.0
75	151.5
76	126.5
77	105.0
78	84.5
79	71.0
80	68.0
81	50.0
82	35.0
83	28.5
84	27.0
85	21.0
86	12.0
87	10.5
88	10.5
89	9.5
90	8.0
91	6.5
92	4.5
93	3.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2226838384996386
2	0.20652056456633697
3	0.1798987016173696
4	0.16399140713550034
5	0.17428543793238813
6	0.13550455104038278
7	0.1204383318989342
8	0.11661692437397939
9	0.10336084563908834
10-11	0.08909913334499868
12-13	0.11693689868826987
14-15	0.11265838500004297
16-17	0.10408307337705829
18-19	0.09665966928551929
20-21	0.10050850318027041
22-23	0.08749926177354632
24-25	0.403323052101509
26-27	0.1933833334338967
28-29	0.09148522751727907
30-31	0.17537335060097575
32-33	0.20663027004552229
34-35	0.20614573751245388
36-37	0.21110076832232347
38-39	0.21190527516968238
40-41	0.2089980799712718
42-43	0.22496936931599912
44-45	0.20671254915491125
46-47	0.22528020150702419
48-49	0.21550727173626658
50-51	0.21849674604406613
52-53	0.2101682717492484
54-55	0.19283480603797015
56-57	0.2007061741695158
58-59	0.19553173240127558
60-61	0.17461455436994405
62-63	0.16326917939753044
64-65	0.16772139342780074
66-67	0.18001754921982033
68-69	0.17305125129155347
70-71	0.16563698932327992
72-73	0.16924812801312955
74-75	0.15930149790032855
76-77	0.17021719307926642
78-79	0.16811450472821474
80-81	0.17342607834543658
82-83	0.19917029746092155
84-85	0.1974058676706912
86-87	0.2015746758797328
88-89	0.2079101673026842
90-91	0.1893059464575095
92-93	0.16962295506701267
94-95	0.17060116225641497
96-97	0.17979813826144972
98-99	0.19363931288532907
100-101	0.20586233169122514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5469189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.99257829792445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.26649578294796	27.598876208235318
2	5.639513975591667	3.6084518485271118
3	2.015538508115792	1.9344682060012892
4	1.166950269412022	1.4933499145579259
5	0.7668510386388888	1.2266770948249672
6	0.596616664834747	1.1452383218143316
7	0.391953023579771	0.8777711457188839
8	0.274613209470386	0.7028467684500521
9	0.2515053783737873	0.7241674958975256
>10	2.0145827998714076	13.325722299824802
>50	0.2831477366241527	6.396762234638931
>100	0.26541301973775394	18.291084266952833
>500	0.04426595948498952	9.664791085682216
>1k	0.021976099581932033	11.347391269470284
>5k	5.188826779744832E-4	1.1343299756451037
>10k+	5.765105642943993E-5	0.5280718637585994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	28631	0.5234962624257454	Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC	9459	0.1729506879356336	No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG	9135	0.16702659205962714	No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG	8295	0.15166782497368442	No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC	8277	0.1513387085361285	No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA	5476	0.10012453400312185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.005814390396821174	0.0	0.0
2	0.006417770532340352	0.0	0.0
3	0.007130856147044836	0.0	0.0
4	0.007642815049909594	0.0	0.0
5	0.00850217463686115	0.0	0.0
6	0.009379818470343592	0.0	0.0
7	0.010933979425468749	0.0	0.0
8	0.012268729422223295	0.0	0.0
9	0.013402352707138115	0.0	0.0
10-11	0.01922588522722473	0.0	0.0
12-13	0.025268828705681957	0.0	0.0
14-15	0.031558609512306116	0.0	0.0
16-17	0.036230234500947034	0.0	0.0
18-19	0.041176123187551206	0.0	0.0
20-21	0.047127645433354015	0.0	1.8284246530884194E-5
22-23	0.054075659115090005	0.0	1.8284246530884194E-5
24-25	0.06213901183520994	0.0	1.8284246530884194E-5
26-27	0.07017493818553354	0.0	1.8284246530884194E-5
28-29	0.07907936624607415	0.0	1.8284246530884194E-5
30-31	0.12202906134712112	0.0	1.8284246530884194E-5
32-33	0.1339229637154613	0.0	1.8284246530884194E-5
34-35	0.14338506129519385	0.0	1.8284246530884194E-5
36-37	0.15425504585780453	0.0	1.8284246530884194E-5
38-39	0.16660605438941678	0.0	1.8284246530884194E-5
40-41	0.17953301668675192	0.0	1.8284246530884194E-5
42-43	0.19367588137839084	0.0	1.8284246530884194E-5
44-45	0.2087603847663703	0.0	1.8284246530884194E-5
46-47	0.2251339275347771	0.0	1.8284246530884194E-5
48-49	0.2431164839979017	0.0	1.8284246530884194E-5
50-51	0.26248864319737353	0.0	1.8284246530884194E-5
52-53	0.2820070763690924	0.0	1.8284246530884194E-5
54-55	0.3024031533742937	0.0	1.8284246530884194E-5
56-57	0.3256881413313747	0.0	1.8284246530884194E-5
58-59	0.34836060702967114	0.0	1.8284246530884194E-5
60-61	0.3752567336766018	0.0	1.8284246530884194E-5
62-63	0.40400871134641714	0.0	1.8284246530884194E-5
64-65	0.43503707770932765	0.0	1.8284246530884194E-5
66-67	0.4668973772893934	0.0	1.8284246530884194E-5
68-69	0.5005861015225475	0.0	1.8284246530884194E-5
70-71	0.5376665534871806	0.0	1.8284246530884194E-5
72-73	0.5784495653743178	0.0	1.8284246530884194E-5
74-75	0.6222769043088472	0.0	1.8284246530884194E-5
76-77	0.6687188904972932	0.0	1.8284246530884194E-5
78-79	0.7206918612613314	0.0	1.8284246530884194E-5
80-81	0.7759559964009289	0.0	3.656849306176839E-5
82-83	0.8350689654352775	0.0	3.656849306176839E-5
84-85	0.8996854926754223	0.0	3.656849306176839E-5
86-87	0.9710946175017905	0.0	3.656849306176839E-5
88-89	1.0484369803274305	0.0	3.656849306176839E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	5375	0.0	90.64289	9
CGGGAGA	7065	0.0	90.51142	4
ATTCGGA	1200	0.0	89.82476	2
AGAGCGG	5815	0.0	87.049126	8
GAGAGCG	6280	0.0	86.727295	7
CGGAAAG	3350	0.0	86.58535	5
CGGAGAG	2545	0.0	85.806076	5
AAGCGGC	1945	0.0	85.61095	9
GTTCGGA	1290	0.0	83.92601	2
TCGGGAG	7685	0.0	82.53354	3
TTCGGAG	1115	0.0	82.17701	3
CCGGAGA	1185	0.0	80.12345	4
TCGGAGA	1650	0.0	79.985054	4
TCGGGAA	2820	0.0	79.630585	3
GGATCGG	2275	0.0	78.91388	1
AAGGGGC	5155	0.0	77.48635	9
GAACCGG	670	0.0	77.26711	1
AAAGCGG	2310	0.0	77.219246	8
GGAAAGC	2625	0.0	76.64322	6
GATCCGG	1525	0.0	75.99114	1
>>END_MODULE