FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469189
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT286310.5234962624257454Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC94590.1729506879356336No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG91350.16702659205962714No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG82950.15166782497368442No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82770.1513387085361285No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA54760.10012453400312185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC53750.090.642899
CGGGAGA70650.090.511424
ATTCGGA12000.089.824762
AGAGCGG58150.087.0491268
GAGAGCG62800.086.7272957
CGGAAAG33500.086.585355
CGGAGAG25450.085.8060765
AAGCGGC19450.085.610959
GTTCGGA12900.083.926012
TCGGGAG76850.082.533543
TTCGGAG11150.082.177013
CCGGAGA11850.080.123454
TCGGAGA16500.079.9850544
TCGGGAA28200.079.6305853
GGATCGG22750.078.913881
AAGGGGC51550.077.486359
GAACCGG6700.077.267111
AAAGCGG23100.077.2192468
GGAAAGC26250.076.643226
GATCCGG15250.075.991141
Produced by FastQC (version 0.11.2)