##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2882654 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.707829312848506 33.0 33.0 33.0 22.0 33.0 2 30.070563446046595 33.0 33.0 33.0 15.0 33.0 3 29.883492781305005 33.0 33.0 33.0 15.0 33.0 4 33.12757410358649 37.0 37.0 37.0 15.0 37.0 5 33.208129383547245 37.0 37.0 37.0 15.0 37.0 6 33.99682167891117 37.0 37.0 37.0 27.0 37.0 7 33.19666945807579 37.0 37.0 37.0 15.0 37.0 8 33.6442313229406 37.0 37.0 37.0 22.0 37.0 9 32.885036150713894 37.0 37.0 37.0 15.0 37.0 10-11 32.98075367352447 37.0 37.0 37.0 15.0 37.0 12-13 32.95962713527187 37.0 37.0 37.0 15.0 37.0 14-15 34.49238774407196 40.0 37.0 40.0 15.0 40.0 16-17 34.40081327831922 40.0 37.0 40.0 15.0 40.0 18-19 34.46182302836206 40.0 37.0 40.0 15.0 40.0 20-21 34.40250876449272 40.0 37.0 40.0 15.0 40.0 22-23 35.43839132271858 40.0 37.0 40.0 24.5 40.0 24-25 34.616757682330245 40.0 37.0 40.0 15.0 40.0 26-27 34.47097050148925 37.0 37.0 40.0 18.5 40.0 28-29 34.07759099774027 37.0 37.0 40.0 15.0 40.0 30-31 34.04335709384477 37.0 37.0 40.0 10.5 40.0 32-33 33.955964364783284 37.0 37.0 40.0 10.5 40.0 34-35 34.049139265413054 37.0 37.0 40.0 15.0 40.0 36-37 33.89067661259381 37.0 33.0 40.0 10.5 40.0 38-39 33.714934050357755 37.0 33.0 40.0 15.0 40.0 40-41 33.75096438906647 37.0 33.0 40.0 15.0 40.0 42-43 33.617417837867464 37.0 33.0 40.0 15.0 40.0 44-45 33.405873545697816 37.0 33.0 40.0 6.0 40.0 46-47 33.31818039903506 37.0 33.0 40.0 6.0 40.0 48-49 33.21991990714113 37.0 33.0 40.0 10.5 40.0 50-51 32.36209427145956 37.0 33.0 38.5 15.0 38.5 52-53 32.75005619821179 37.0 33.0 38.5 10.5 40.0 54-55 33.33992390345841 37.0 33.0 40.0 10.5 40.0 56-57 33.72078664314205 37.0 33.0 40.0 18.5 40.0 58-59 33.06168985247622 37.0 33.0 40.0 6.0 40.0 60-61 32.927298593587714 37.0 33.0 40.0 10.5 40.0 62-63 32.52243470773808 37.0 33.0 40.0 6.0 40.0 64-65 32.35954332361775 37.0 33.0 40.0 6.0 40.0 66-67 32.279889643363376 37.0 33.0 37.0 10.5 40.0 68-69 32.4772806587263 37.0 33.0 37.0 15.0 40.0 70-71 32.00711896051347 37.0 33.0 37.0 15.0 40.0 72-73 32.46976761692524 37.0 33.0 37.0 18.5 40.0 74-75 32.63884219195228 37.0 33.0 37.0 22.0 37.0 76-77 32.327114527098985 37.0 33.0 37.0 22.0 37.0 78-79 31.77260208821454 37.0 33.0 37.0 18.5 37.0 80-81 31.131170268787027 37.0 33.0 37.0 15.0 37.0 82-83 30.761799369608703 35.0 30.0 37.0 15.0 37.0 84-85 30.478662371550662 33.0 27.0 37.0 15.0 37.0 86-87 30.28292434679986 33.0 27.0 37.0 6.0 37.0 88-89 30.096098768704117 33.0 27.0 37.0 6.0 37.0 90-91 29.94919438822696 33.0 27.0 37.0 6.0 37.0 92-93 29.75972211718784 33.0 27.0 37.0 6.0 37.0 94-95 29.6383351938873 33.0 27.0 37.0 6.0 37.0 96-97 29.50915961471616 33.0 27.0 37.0 6.0 37.0 98-99 29.36296291542447 33.0 27.0 37.0 6.0 37.0 100-101 28.19088641925115 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.2187859716728795 2306 2 -0.16631954687714057 2306 3 -0.16757383104701162 2306 4 0.08561349968962872 2306 5 -0.06088725674369755 2306 6 -0.36369881049782293 2306 7 0.24158977755620725 2306 8 -0.4394118796908515 2306 9 0.0919745086799324 2306 10-11 -0.11111817127212475 2306 12-13 -0.07039613604018058 2306 14-15 0.2984465819991158 2306 16-17 0.22448802708827742 2306 18-19 0.3308391270102504 2306 20-21 0.1565619995364287 2306 22-23 -0.05228734000918678 2306 24-25 0.30024216987821006 2306 26-27 -0.1473775407302682 2306 28-29 0.1103860795906968 2306 30-31 0.17088355280078105 2306 32-33 0.2923579166742414 2306 34-35 0.26775656444767293 2306 36-37 0.11393742287118158 2306 38-39 0.13231712846787502 2306 40-41 0.24379449171183154 2306 42-43 0.1360482208430156 2306 44-45 0.2970324803361706 2306 46-47 0.18574084105552657 2306 48-49 0.1914410611960662 2306 50-51 0.35829383482308685 2306 52-53 0.21351980869497567 2306 54-55 0.24454949006423732 2306 56-57 -0.014702843374983843 2306 58-59 0.4932096548128371 2306 60-61 0.38021126106266934 2306 62-63 0.4431242918074716 2306 64-65 0.28286527756865354 2306 66-67 0.24929077421971613 2306 68-69 0.03666183073037388 2306 70-71 0.3292705112368921 2306 72-73 -0.22649975886232454 2306 74-75 -0.12815374089789344 2306 76-77 -0.03186359065541211 2306 78-79 -0.014672438320772585 2306 80-81 0.3395327776456689 2306 82-83 0.40856268249780214 2306 84-85 0.5502917971130152 2306 86-87 0.524484607358648 2306 88-89 0.42001679157022664 2306 90-91 0.3598927797885416 2306 92-93 0.39388922663038883 2306 94-95 0.39335446522603235 2306 96-97 0.27911727667070707 2306 98-99 0.21845236005866653 2306 100-101 0.20424212537713515 2307 1 -0.028801211018812722 2307 2 0.039354193916775415 2307 3 0.08699263161168957 2307 4 0.2441454263643621 2307 5 0.16465467105805942 2307 6 -0.14367891604621974 2307 7 0.5354582683709452 2307 8 -0.1437074151995077 2307 9 0.40797131380740836 2307 10-11 0.222788932419121 2307 12-13 0.2495735812723936 2307 14-15 0.5954450678647447 2307 16-17 0.5131784723613819 2307 18-19 0.5183492334896656 2307 20-21 0.4295759109522166 2307 22-23 0.20672803163975573 2307 24-25 0.346176302583018 2307 26-27 -0.03392242329910289 2307 28-29 0.35970883521725483 2307 30-31 0.47478698140860587 2307 32-33 0.3937085833343801 2307 34-35 0.2355864521861193 2307 36-37 0.21365733251312946 2307 38-39 0.22255022675920344 2307 40-41 0.35424946703977156 2307 42-43 0.3428897561458726 2307 44-45 0.37765136438484603 2307 46-47 0.28711375422064833 2307 48-49 0.3132668426413048 2307 50-51 0.23848209709664303 2307 52-53 0.20168378601725578 2307 54-55 0.3309542294518195 2307 56-57 0.2975685051846284 2307 58-59 0.3733029529307075 2307 60-61 0.25202925133104515 2307 62-63 0.3066181691184866 2307 64-65 0.4410351840255302 2307 66-67 0.27034057405016654 2307 68-69 0.14248247685904403 2307 70-71 0.2522391946260072 2307 72-73 -0.19080607461568633 2307 74-75 -0.2468393350608764 2307 76-77 0.13422087746200617 2307 78-79 0.29184314158289126 2307 80-81 0.33818181697886374 2307 82-83 0.39721569092109377 2307 84-85 0.4282415905733252 2307 86-87 0.4379140482069559 2307 88-89 0.36168792550496676 2307 90-91 0.3488873259647427 2307 92-93 0.31959844422963 2307 94-95 0.15172723628793605 2307 96-97 0.33737891320985725 2307 98-99 0.1727587248131499 2307 100-101 0.11004767916320546 2201 1 -0.11628078384760698 2201 2 -0.2533997584553269 2201 3 -0.08330067018425069 2201 4 0.14813721715049866 2201 5 0.06921668883967413 2201 6 -0.22298229974165196 2201 7 0.3450305229013466 2201 8 -0.16878125757724405 2201 9 0.1484734474801428 2201 10-11 0.09621587740598159 2201 12-13 0.05152574641952867 2201 14-15 0.40009883935306334 2201 16-17 0.24187903548806133 2201 18-19 0.19184795222189877 2201 20-21 0.19800806742484411 2201 22-23 -0.0018147870319182857 2201 24-25 0.30353165289192674 2201 26-27 -0.09803709176475905 2201 28-29 0.21767626219269687 2201 30-31 0.3082618929803118 2201 32-33 0.3993536104247468 2201 34-35 0.3658879074208272 2201 36-37 0.24672948916190052 2201 38-39 0.33100729121668593 2201 40-41 0.32967594931346866 2201 42-43 0.33459137551347595 2201 44-45 0.36531295851422385 2201 46-47 0.3783347536920729 2201 48-49 0.23097848207878258 2201 50-51 0.14803928868098382 2201 52-53 0.23688539556918187 2201 54-55 0.32460073017900726 2201 56-57 0.5320305993084062 2201 58-59 0.5412694790649226 2201 60-61 0.37380151976852716 2201 62-63 0.45605892284554983 2201 64-65 0.4940429020882817 2201 66-67 0.5114020182105747 2201 68-69 0.279716006279763 2201 70-71 0.47690973166865547 2201 72-73 0.015924787232506787 2201 74-75 0.20122707620231495 2201 76-77 0.4696361753606695 2201 78-79 0.4347677492445179 2201 80-81 0.5672118935173422 2201 82-83 0.6311440687225307 2201 84-85 0.6075755037964505 2201 86-87 0.5643831670576347 2201 88-89 0.4850164843186171 2201 90-91 0.480139526466683 2201 92-93 0.4853601109950638 2201 94-95 0.3310800469951367 2201 96-97 0.43384937690091263 2201 98-99 0.3786402089665053 2201 100-101 0.35683093291000034 1101 1 -0.1168373451331064 1101 2 -0.07343007393482992 1101 3 -0.029184151210344567 1101 4 0.06116740817411426 1101 5 0.07106241563568005 1101 6 0.008420015069773967 1101 7 0.24160614721958495 1101 8 -0.0635436736695496 1101 9 0.12803305750927763 1101 10-11 0.09741642555670182 1101 12-13 0.043982885326776966 1101 14-15 0.24476690902861264 1101 16-17 0.2307960768702415 1101 18-19 0.25737116835274065 1101 20-21 0.20150772955519614 1101 22-23 0.14441126265815996 1101 24-25 0.16455903199562272 1101 26-27 0.04516498629104149 1101 28-29 0.33691666585200153 1101 30-31 0.46252683964730323 1101 32-33 0.34240125081090156 1101 34-35 0.107381790098934 1101 36-37 0.257894425222041 1101 38-39 0.1830676320623823 1101 40-41 0.44948176748154367 1101 42-43 0.22637102151053767 1101 44-45 0.34571685958489695 1101 46-47 0.28439243550620574 1101 48-49 0.26271039217701997 1101 50-51 0.24287161118620304 1101 52-53 0.2092381413538149 1101 54-55 0.37676562418634774 1101 56-57 0.42161370953768085 1101 58-59 0.4453561269230235 1101 60-61 0.3774102297738793 1101 62-63 0.42687640053841136 1101 64-65 0.32371659100678585 1101 66-67 0.34548146260952706 1101 68-69 0.26004780465714106 1101 70-71 0.31425116320421154 1101 72-73 0.04929369236290171 1101 74-75 0.10691462518234829 1101 76-77 0.3538554240727336 1101 78-79 0.42189584363758925 1101 80-81 0.5490035373344675 1101 82-83 0.4887898570484346 1101 84-85 0.4368473925822549 1101 86-87 0.40981593389251003 1101 88-89 0.491296399062076 1101 90-91 0.5868553432778292 1101 92-93 0.5566133205013166 1101 94-95 0.4543049970127342 1101 96-97 0.3518141332406266 1101 98-99 0.32951852532860926 1101 100-101 0.42829398083300774 2304 1 0.12808419162060858 2304 2 -0.009716697087423398 2304 3 0.05015404900715126 2304 4 0.14073621640546463 2304 5 0.16397370615882068 2304 6 0.0783055475821115 2304 7 0.5675965747261458 2304 8 0.05524884468300684 2304 9 0.31400146421137975 2304 10-11 0.22397387747380293 2304 12-13 0.22224005395775492 2304 14-15 0.4461035311638213 2304 16-17 0.35294315052237835 2304 18-19 0.27990518868694636 2304 20-21 0.3980728005802092 2304 22-23 0.11415526515415308 2304 24-25 0.3288463288853549 2304 26-27 0.29287981913414995 2304 28-29 0.33001707755513365 2304 30-31 0.5404231745624841 2304 32-33 0.5396824501534425 2304 34-35 0.2903918577213389 2304 36-37 0.4019481297589209 2304 38-39 0.4374768798959039 2304 40-41 0.5653685118163878 2304 42-43 0.45689481822279276 2304 44-45 0.48427666732111874 2304 46-47 0.5177259195637376 2304 48-49 0.5257702666086956 2304 50-51 0.35567566898290437 2304 52-53 0.2602556426933731 2304 54-55 0.5499748512199361 2304 56-57 0.7741670150322548 2304 58-59 0.7654219632501196 2304 60-61 0.5966042773740838 2304 62-63 0.698444467206734 2304 64-65 0.6861391281923233 2304 66-67 0.625192026943509 2304 68-69 0.6002194945843158 2304 70-71 0.6641502156765782 2304 72-73 0.4289044396006929 2304 74-75 0.44537540712438073 2304 76-77 0.6630521620997669 2304 78-79 0.6340213034405799 2304 80-81 0.6724398680444459 2304 82-83 0.6670379822278889 2304 84-85 0.6646762407404445 2304 86-87 0.711754171640326 2304 88-89 0.7492048974195562 2304 90-91 0.6413573995326196 2304 92-93 0.6922554846896993 2304 94-95 0.6962252382920191 2304 96-97 0.5083048444854796 2304 98-99 0.7109385280868601 2304 100-101 0.7158989131314684 2202 1 -0.056678480179122914 2202 2 -0.21916915991804942 2202 3 -0.18970446295163867 2202 4 -0.09309526538378066 2202 5 -0.017624630221362736 2202 6 -0.01793821947458696 2202 7 0.1246587102690313 2202 8 0.05953360321523604 2202 9 0.01012481850432323 2202 10-11 -0.024163620753185455 2202 12-13 -0.12900848273496024 2202 14-15 -0.014550015503431268 2202 16-17 0.07024998939512272 2202 18-19 0.02836396009931974 2202 20-21 0.08568994886748982 2202 22-23 0.12197418091574264 2202 24-25 0.054439154768374465 2202 26-27 0.014853109172456413 2202 28-29 0.05317638461559682 2202 30-31 0.2400896652974609 2202 32-33 0.037131440616001044 2202 34-35 0.055448779842045326 2202 36-37 -0.0037565923329268003 2202 38-39 -0.10468995935426761 2202 40-41 0.015121294306979394 2202 42-43 -0.09624979336690842 2202 44-45 0.02142093069998907 2202 46-47 -0.025700292310382622 2202 48-49 -0.008582679151381 2202 50-51 -0.029862045840957308 2202 52-53 -0.0908509006871796 2202 54-55 0.03006093599709203 2202 56-57 0.2698025089163494 2202 58-59 0.11701171002415123 2202 60-61 0.09709904721098894 2202 62-63 0.17447894860089264 2202 64-65 0.16428935559468982 2202 66-67 0.26713511638925524 2202 68-69 0.3515974232534589 2202 70-71 0.3196167603477065 2202 72-73 0.28854382224481157 2202 74-75 0.37615937457275805 2202 76-77 0.41111706567519946 2202 78-79 0.39718239416836454 2202 80-81 0.3041909423539728 2202 82-83 0.40801356911835995 2202 84-85 0.30999551414864257 2202 86-87 0.1260656973577028 2202 88-89 0.21242665109936 2202 90-91 0.22793527243932132 2202 92-93 0.3178894323229322 2202 94-95 0.3873176462611774 2202 96-97 0.17738920221000853 2202 98-99 0.31660164142035185 2202 100-101 0.39408271365780223 1102 1 0.08324615646698419 1102 2 -0.11207999614593689 1102 3 -0.010988917635440743 1102 4 -0.08671157643788518 1102 5 0.05121530258070095 1102 6 0.1561366947813383 1102 7 -0.009337516963036308 1102 8 0.14475224233339645 1102 9 -0.016711947610801303 1102 10-11 0.09372078568564746 1102 12-13 -0.009878850118376192 1102 14-15 -0.025396004515194193 1102 16-17 -0.02954205122035347 1102 18-19 0.1058616793889442 1102 20-21 -0.11776149263059921 1102 22-23 0.1473242085027806 1102 24-25 -0.03605890120095978 1102 26-27 0.06375583806075014 1102 28-29 -0.05434225424379946 1102 30-31 0.02837030724878531 1102 32-33 0.13486667292644228 1102 34-35 -0.005795445784549713 1102 36-37 0.167314943337729 1102 38-39 -0.0041702909047245384 1102 40-41 0.015611938147287674 1102 42-43 0.07627742924393743 1102 44-45 0.1031991688846432 1102 46-47 0.11165036895906155 1102 48-49 -0.03280945867332008 1102 50-51 0.06687170088101624 1102 52-53 0.13777913775641082 1102 54-55 0.2175210253624158 1102 56-57 0.3363112725082473 1102 58-59 0.3935061180069326 1102 60-61 0.3416625754534195 1102 62-63 0.2435962612124669 1102 64-65 0.24572962024622313 1102 66-67 0.31199646673655934 1102 68-69 0.3678673771332228 1102 70-71 0.35356685584570613 1102 72-73 0.44453815878019753 1102 74-75 0.48239526658569076 1102 76-77 0.4576264502988536 1102 78-79 0.22278136636080248 1102 80-81 0.2964304173751273 1102 82-83 0.26854370885337886 1102 84-85 0.31859759466253834 1102 86-87 0.25795674193599893 1102 88-89 0.22128404466932494 1102 90-91 0.2939142183126684 1102 92-93 0.3168772368117807 1102 94-95 0.303800303518063 1102 96-97 0.04064253745243818 1102 98-99 0.3421307906672233 1102 100-101 0.35974418579633394 2305 1 0.19560379973355424 2305 2 0.09593539306861487 2305 3 0.13128320111106717 2305 4 0.24982117113480484 2305 5 0.126466482117209 2305 6 0.4014852266970621 2305 7 0.2094665694973159 2305 8 0.2976164039201521 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-0.24262590686976893 1213 66-67 -0.19116787924211565 1213 68-69 -0.01721368554621705 1213 70-71 -0.17650434775853086 1213 72-73 0.2137505520138987 1213 74-75 0.10610398659688514 1213 76-77 0.08142642355607421 1213 78-79 0.02661603261229928 1213 80-81 -0.23164871855719937 1213 82-83 -0.1511817883464488 1213 84-85 -0.20451024134681361 1213 86-87 -0.20799574082272443 1213 88-89 -0.1896389130085403 1213 90-91 -0.269238987320751 1213 92-93 -0.1293749737515455 1213 94-95 -0.20279833043946738 1213 96-97 -0.13814673496494478 1213 98-99 -0.22205292483718964 1213 100-101 -0.10829012803140259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 430.0 3 269.0 4 263.0 5 205.0 6 652.0 7 4378.0 8 23834.0 9 74629.0 10 91044.0 11 62795.0 12 35679.0 13 21747.0 14 15275.0 15 11721.0 16 10136.0 17 9520.0 18 9141.0 19 9417.0 20 9929.0 21 10781.0 22 11497.0 23 12084.0 24 13036.0 25 15048.0 26 17710.0 27 21331.0 28 26809.0 29 33401.0 30 39844.0 31 50801.0 32 69699.0 33 99500.0 34 152212.0 35 257267.0 36 507600.0 37 870804.0 38 281766.0 39 400.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.414948031590235 21.900711951743446 23.425781490367147 13.258558526299174 2 30.87887802154945 26.796905577426955 30.900324583460492 11.423891817563101 3 36.04870013657167 19.865687150100257 29.79611552642644 14.289497186901631 4 26.98417772317374 17.69493977611572 35.45624291631893 19.864639584391615 5 35.20111976837364 16.230212757380972 31.7729758581219 16.7956916161235 6 31.61375950836966 16.24874822458909 37.420712206803195 14.716780060238055 7 36.3103087674136 19.069651618612525 28.579436506329518 16.04060310764436 8 27.057915658642063 33.35399740362035 24.206933820102424 15.381153117635169 9 33.77088061004978 27.451858050917348 22.46714575668501 16.310115582347866 10-11 27.123892422643188 30.820857174601414 26.52477640131104 15.530474001444365 12-13 29.78514176802215 23.698950505784737 26.548185429745985 19.96772229644713 14-15 25.806053355165997 24.097774683080527 28.961730036091616 21.13444192566186 16-17 27.819216632028184 25.05428432031308 30.09738709503793 17.029111952620806 18-19 27.696084516456665 25.18290052215128 30.554747757093313 16.566267204298743 20-21 25.731582541366134 28.703506127071606 28.98798754011344 16.57692379144882 22-23 29.81258849254875 25.761443878971708 27.82339404469017 16.60257358378938 24-25 24.604490859363654 30.800101278201957 27.835937671416318 16.75947019101807 26-27 22.54199472306152 33.15167175169647 27.64451236455536 16.661821160686653 28-29 24.59016706506894 30.934841133586254 27.483038028392258 16.991953772952545 30-31 28.033532707734178 26.997711341266612 27.870362390657995 17.098393560341222 32-33 27.69969200008412 26.570793487803147 28.878872311170817 16.850642200941916 34-35 23.12887725248458 26.595581785358046 28.123636177823553 22.151904784333816 36-37 19.34344833627715 26.810447287488127 29.25909329981287 24.587011076421852 38-39 19.202538133975107 29.01136984041759 31.334232426702137 20.451859598905166 40-41 21.429266661429246 26.673495012051863 34.015658034423765 17.881580292095133 42-43 23.180297655294844 26.210618596146052 33.163534488438316 17.445549260120785 44-45 21.044086760832066 28.90985483084919 32.69569956986661 17.35035883845213 46-47 24.09785270325194 30.416751703015866 28.169149728063314 17.316245865668876 48-49 20.200676163078715 29.32826935277809 31.084051068563635 19.387003415579564 50-51 19.11310229767316 27.018601204573617 32.90228748700439 20.966009010748827 52-53 25.95522807803053 26.415013336605636 28.077926240384276 19.55183234497956 54-55 27.93449560524694 26.568384123209487 27.720119102691143 17.77700116885243 56-57 28.80866505047991 26.348183215027515 27.170950766553243 17.672200967939332 58-59 26.38706243156035 26.32682771776995 27.644640214022665 19.641469636647034 60-61 21.29850696736401 26.50904821667139 27.276927329834784 24.915517486129822 62-63 24.601837712189045 26.62319950938748 27.528782659001717 21.24618011942176 64-65 21.080009916955056 28.612691304890948 30.706423209010598 19.600875569143398 66-67 19.23268192953051 28.699797638876966 28.986293448302348 23.081226983290176 68-69 18.72150537783869 27.10007102805817 35.37291120519742 18.805512388905722 70-71 18.217495401675603 32.292963343184674 31.46178156990539 18.027759685234336 72-73 18.08015311406466 36.599418073429355 27.05260116520832 18.267827647297665 74-75 18.529511961951904 36.90814557215903 26.410556238190097 18.15178622769897 76-77 18.74802476387947 36.51650615457079 26.578290758579282 18.15717832297046 78-79 18.995338781411625 36.056903386519764 26.80446527679688 18.143292555271724 80-81 18.52968025739813 36.09055568625312 26.717965362173747 18.66179869417501 82-83 18.353928031492586 35.450710802540634 26.884114655067986 19.311246510898787 84-85 18.598834041673147 34.17364941132921 27.546856005276272 19.680660541721373 86-87 18.7765138289458 33.80559789928008 27.82379827582881 19.594089995945314 88-89 19.230043246974954 34.003416375450904 27.308078295761202 19.458462081812947 90-91 19.76532702543203 33.33392997033961 26.945911321899686 19.954831682328678 92-93 19.60785608266459 33.18736770706846 27.210728577775082 19.994047632491867 94-95 19.106367526639225 33.33997612763895 27.29910469958875 20.254551646133077 96-97 19.474017921263417 33.90110586104792 26.820592580828396 19.804283636860273 98-99 19.966549732074615 32.989614220117645 26.78409249291041 20.259743554897334 100-101 20.158258984320618 32.87831442084939 26.284016270141496 20.679410324688497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 200.0 1 239.0 2 269.0 3 296.0 4 307.0 5 290.0 6 329.5 7 424.5 8 509.0 9 641.5 10 774.5 11 843.5 12 900.5 13 914.0 14 892.0 15 860.5 16 868.5 17 849.5 18 886.0 19 1007.5 20 1218.5 21 1478.5 22 1931.0 23 2743.0 24 3822.5 25 5465.5 26 8255.0 27 12343.0 28 16766.0 29 21166.0 30 25820.0 31 30602.0 32 37372.0 33 48391.5 34 64589.0 35 87859.5 36 113589.5 37 141456.5 38 172846.0 39 188416.5 40 183917.5 41 169121.0 42 155020.5 43 152259.5 44 146884.0 45 139781.0 46 142260.5 47 134692.0 48 112166.5 49 93404.0 50 81136.0 51 70643.5 52 61291.5 53 52142.0 54 43611.0 55 35542.0 56 27423.0 57 20754.5 58 15683.5 59 12000.0 60 9064.5 61 6780.5 62 4957.0 63 3592.5 64 2614.0 65 1904.0 66 1425.5 67 1013.5 68 724.5 69 522.5 70 400.5 71 316.5 72 237.0 73 193.0 74 153.5 75 120.0 76 90.0 77 69.5 78 69.5 79 55.0 80 36.0 81 35.0 82 34.0 83 28.0 84 24.0 85 22.0 86 18.5 87 14.5 88 11.0 89 9.0 90 9.5 91 10.0 92 9.0 93 7.0 94 5.0 95 3.0 96 2.5 97 2.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21490612470313813 2 0.19898329803021797 3 0.17497764213117495 4 0.1581875591035206 5 0.17053728959493578 6 0.12984562143080647 7 0.11680208585560391 8 0.11309022865734146 9 0.10022014435308574 10-11 0.08651749394828516 12-13 0.11333306043666705 14-15 0.1101935924325292 16-17 0.10091394943687311 18-19 0.09510333186015386 20-21 0.09895395007517378 22-23 0.08561554733936157 24-25 0.39390783632027987 26-27 0.1879344520709041 28-29 0.09048952805296785 30-31 0.17296560738819158 32-33 0.20354506645611994 34-35 0.20323285416841563 36-37 0.20824559589877936 38-39 0.20996276348115311 40-41 0.2053663048010618 42-43 0.222277803718379 44-45 0.20370117259997209 46-47 0.22316240520020786 48-49 0.2126165679266398 50-51 0.2150969211011797 52-53 0.20762117132337074 54-55 0.18985976117841405 56-57 0.19834152832771465 58-59 0.19341551223282433 60-61 0.17301764276947562 62-63 0.16023428410069332 64-65 0.16493481354335277 66-67 0.1777875527205138 68-69 0.1706066701033145 70-71 0.16316561057969495 72-73 0.16649587498187435 74-75 0.15636632075857873 76-77 0.16767534362431286 78-79 0.16507357456011024 80-81 0.17112702391615503 82-83 0.19632949358473128 84-85 0.19501126392553528 86-87 0.19896595290312327 88-89 0.20540099505525117 90-91 0.18788241668962005 92-93 0.1667733970153893 94-95 0.1682130425642481 96-97 0.17770082708504037 98-99 0.19242683998842733 100-101 0.2029553321349007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2882654.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.28729185101985 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2619878675064 56.54487377191118 2 3.7375551774151563 4.5812926993506595 3 1.2288185631410538 2.2593288573352988 4 0.5206654474145315 1.2764070092974489 5 0.43232688258233637 1.324807191393262 6 0.25320200146357924 0.9310838976576443 7 0.15214898557325368 0.6527359498565225 8 0.15713877825346223 0.7704488131146106 9 0.122931995214037 0.6780752161659775 >10 0.955062271322253 11.446811368260247 >50 0.09123785677087601 3.9438094286660195 >100 0.07668984110091966 9.931693545795879 >500 0.008154187031365197 3.297854653653899 >1k 0.0020235611012996767 1.932875042241475 >5k 0.0 0.0 >10k+ 5.658410936369736E-5 0.4279025553000965 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 12339 0.4280430464426185 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3438 0.11926509390304907 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3426 0.11884881085277663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005515750416109599 0.0 0.0 2 0.005654511432867073 0.0 0.0 3 0.00620955549989697 0.0 0.0 4 0.006729909312737498 0.0 0.0 5 0.008048138971933502 0.0 0.0 6 0.009019466089235822 0.0 0.0 7 0.010580527527757407 0.0 0.0 8 0.011794686424385307 0.0 0.0 9 0.013286367354528154 0.0 0.0 10-11 0.020276453573685915 0.0 0.0 12-13 0.025844239371079568 0.0 0.0 14-15 0.031065122626579535 0.0 0.0 16-17 0.03508919211254628 0.0 0.0 18-19 0.04032742049514094 0.0 0.0 20-21 0.045600339131924954 0.0 0.0 22-23 0.051688478742159136 0.0 0.0 24-25 0.05782865373367737 0.0 0.0 26-27 0.06520033274891818 0.0 0.0 28-29 0.07262404714544304 0.0 0.0 30-31 0.13867429112200078 0.0 0.0 32-33 0.15015676525868177 0.0 0.0 34-35 0.15959251439819 0.0 0.0 36-37 0.1688374671396567 0.0 0.0 38-39 0.17908843725261514 0.0 0.0 40-41 0.1902760442286865 0.0 0.0 42-43 0.20220949166982927 0.0 0.0 44-45 0.2161723189810501 0.0 1.7345127094684275E-5 46-47 0.23067284523220616 0.0 3.469025418936855E-5 48-49 0.24529478737302501 0.0 3.469025418936855E-5 50-51 0.26182469349425913 0.0 3.469025418936855E-5 52-53 0.27819849347164105 0.0 3.469025418936855E-5 54-55 0.2975209650551194 0.0 3.469025418936855E-5 56-57 0.3177974186288053 0.0 3.469025418936855E-5 58-59 0.34006856181837986 0.0 3.469025418936855E-5 60-61 0.36416094335289634 0.0 3.469025418936855E-5 62-63 0.39071633293485797 0.0 3.469025418936855E-5 64-65 0.41893685471790926 0.0 3.469025418936855E-5 66-67 0.44724410213643395 0.0 3.469025418936855E-5 68-69 0.48004373747248197 0.0 3.469025418936855E-5 70-71 0.5145778855179983 0.0 3.469025418936855E-5 72-73 0.5524596430927888 0.0 3.469025418936855E-5 74-75 0.5924401610460361 0.0 3.469025418936855E-5 76-77 0.6371732438232267 0.0 3.469025418936855E-5 78-79 0.6874220770165271 0.0 3.469025418936855E-5 80-81 0.7416429443145103 0.0 3.469025418936855E-5 82-83 0.7992634565230513 0.0 3.469025418936855E-5 84-85 0.8616538786826307 0.0 3.469025418936855E-5 86-87 0.9334973951088129 0.0 3.469025418936855E-5 88-89 1.0102669276298855 0.0 3.469025418936855E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3330 0.0 87.069984 9 CGGGAGA 4470 0.0 86.87268 4 GAGAGCG 3800 0.0 84.66766 7 ATTCGGA 865 0.0 82.329956 2 AAGCGGC 1275 0.0 81.88116 9 ATCGGGA 5735 0.0 80.79772 2 CGGAAAG 2005 0.0 80.50113 5 GATCGGG 5895 0.0 79.90028 1 TCGGGAG 4800 0.0 78.52795 3 AAGCGTC 480 0.0 78.101135 9 GTTCGGA 650 0.0 76.69352 2 TTCGGAG 875 0.0 75.413956 3 ATCCGGA 855 0.0 74.96359 2 AGAGCGG 4060 0.0 74.10281 8 TCGGAGA 1215 0.0 73.84532 4 AAAGCGG 1575 0.0 72.31063 8 TCGGGAA 1670 0.0 71.91989 3 GATCCGG 945 0.0 71.34721 1 CGTCGCC 20 0.0020776778 71.221596 1 GGACCGG 510 0.0 70.756096 1 >>END_MODULE