FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882654
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT123390.4280430464426185Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC34380.11926509390304907No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG34260.11884881085277663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC33300.087.0699849
CGGGAGA44700.086.872684
GAGAGCG38000.084.667667
ATTCGGA8650.082.3299562
AAGCGGC12750.081.881169
ATCGGGA57350.080.797722
CGGAAAG20050.080.501135
GATCGGG58950.079.900281
TCGGGAG48000.078.527953
AAGCGTC4800.078.1011359
GTTCGGA6500.076.693522
TTCGGAG8750.075.4139563
ATCCGGA8550.074.963592
AGAGCGG40600.074.102818
TCGGAGA12150.073.845324
AAAGCGG15750.072.310638
TCGGGAA16700.071.919893
GATCCGG9450.071.347211
CGTCGCC200.002077677871.2215961
GGACCGG5100.070.7560961
Produced by FastQC (version 0.11.2)