FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057334
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT317950.6286909268796563Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC176850.090.786919
ATCGGGA288700.088.482282
TCGGGAG257300.087.050583
GAGAGCG213350.086.866977
AGAGCGG195000.084.769748
GATCGGG307800.084.043711
TCCGGAG40800.082.98033
CGGGAGA268400.081.679744
GAGCGTC324900.081.188629
ATTGGGA115300.080.193322
GGAGAGC236550.079.314326
GGATCGG62500.078.812471
CGGAGAG102600.078.212535
AGAGCGT342100.078.0076148
CGGAAGA343500.076.967884
CCGGAGA51300.076.91674
GAGGGGC220900.076.829519
AAGAGCG329400.076.722427
TCGGAAG355250.076.027573
TCCGGAA27400.075.558193
Produced by FastQC (version 0.11.2)