##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3105344 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838358648832465 33.0 33.0 33.0 27.0 33.0 2 31.69380268337421 33.0 33.0 33.0 27.0 33.0 3 31.644858025391066 33.0 33.0 33.0 27.0 33.0 4 35.30756560303786 37.0 37.0 37.0 33.0 37.0 5 35.27501172172874 37.0 37.0 37.0 33.0 37.0 6 35.44642139485996 37.0 37.0 37.0 33.0 37.0 7 35.24365963964057 37.0 37.0 37.0 33.0 37.0 8 35.265029574823274 37.0 37.0 37.0 33.0 37.0 9 35.0877603254261 37.0 37.0 37.0 33.0 37.0 10-11 35.04638036880938 37.0 37.0 37.0 33.0 37.0 12-13 35.0628542280662 37.0 37.0 37.0 33.0 37.0 14-15 36.784987750149426 40.0 37.0 40.0 33.0 40.0 16-17 36.72251013092269 40.0 37.0 40.0 33.0 40.0 18-19 36.68153399430143 40.0 37.0 40.0 33.0 40.0 20-21 36.62685535000309 40.0 37.0 40.0 33.0 40.0 22-23 36.82598143716123 40.0 37.0 40.0 33.0 40.0 24-25 36.53166203164609 40.0 37.0 40.0 33.0 40.0 26-27 36.41488012278189 40.0 37.0 40.0 33.0 40.0 28-29 36.292678846530364 40.0 37.0 40.0 33.0 40.0 30-31 36.27070044413759 40.0 37.0 40.0 33.0 40.0 32-33 36.18961731775932 40.0 37.0 40.0 33.0 40.0 34-35 36.14585340625708 40.0 37.0 40.0 33.0 40.0 36-37 36.041039414634895 37.0 37.0 40.0 33.0 40.0 38-39 35.943616874652214 37.0 37.0 40.0 27.0 40.0 40-41 35.853121103491276 37.0 37.0 40.0 27.0 40.0 42-43 35.75340751298407 37.0 37.0 40.0 27.0 40.0 44-45 35.59714559804003 37.0 37.0 40.0 27.0 40.0 46-47 35.458984737278705 37.0 37.0 40.0 27.0 40.0 48-49 35.32705684136765 37.0 37.0 40.0 27.0 40.0 50-51 34.1519823568661 37.0 35.0 38.5 24.5 38.5 52-53 34.58162074797511 37.0 33.0 38.5 27.0 40.0 54-55 35.280909619030936 37.0 37.0 40.0 27.0 40.0 56-57 35.33845348534655 37.0 37.0 40.0 27.0 40.0 58-59 35.01734204004452 37.0 35.0 40.0 27.0 40.0 60-61 34.783818636518205 37.0 33.0 40.0 27.0 40.0 62-63 34.50099264364914 37.0 33.0 38.5 27.0 40.0 64-65 34.25755504060098 37.0 33.0 37.0 27.0 40.0 66-67 34.02705481260691 37.0 33.0 37.0 27.0 40.0 68-69 33.886842971342304 37.0 33.0 37.0 27.0 40.0 70-71 33.550267538797634 37.0 33.0 37.0 27.0 38.5 72-73 33.4420180179716 37.0 33.0 37.0 27.0 37.0 74-75 33.28587654057006 37.0 33.0 37.0 27.0 37.0 76-77 33.037878734207865 37.0 33.0 37.0 27.0 37.0 78-79 32.79842410373859 37.0 33.0 37.0 22.0 37.0 80-81 32.5618342122483 37.0 33.0 37.0 22.0 37.0 82-83 32.33434959218689 37.0 33.0 37.0 22.0 37.0 84-85 32.17874863461182 33.0 33.0 37.0 22.0 37.0 86-87 32.03626554739185 33.0 33.0 37.0 22.0 37.0 88-89 31.876151563240658 33.0 33.0 37.0 22.0 37.0 90-91 31.748298900218465 33.0 33.0 37.0 22.0 37.0 92-93 31.57541579934461 33.0 33.0 37.0 18.5 37.0 94-95 31.442960908678717 33.0 33.0 37.0 15.0 37.0 96-97 31.289117083324747 33.0 33.0 37.0 15.0 37.0 98-99 31.126599983769914 33.0 33.0 37.0 15.0 37.0 100-101 29.71820529384184 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07024758132629927 2306 2 0.04710804889814568 2306 3 -0.006649669193365071 2306 4 0.07063286577393768 2306 5 0.08332129481822648 2306 6 0.02966635016596797 2306 7 0.19876044344289312 2306 8 -0.0017939958083914576 2306 9 0.10242743677257948 2306 10-11 0.06652310742837386 2306 12-13 0.03451861977847415 2306 14-15 0.2638345657393941 2306 16-17 0.13116667557023476 2306 18-19 0.18939687732586208 2306 20-21 0.18620490425588798 2306 22-23 0.1527476137386543 2306 24-25 0.1321327927068765 2306 26-27 0.17887754073239392 2306 28-29 0.15729137789679015 2306 30-31 0.13177988618205205 2306 32-33 0.19361802230664438 2306 34-35 0.1989217468854534 2306 36-37 0.1558809626148019 2306 38-39 0.19884193863832422 2306 40-41 0.05147050621008731 2306 42-43 0.0656783495339468 2306 44-45 0.2801889118908534 2306 46-47 0.23610515934242926 2306 48-49 0.2532085858686557 2306 50-51 0.28824212762648216 2306 52-53 0.256977506984164 2306 54-55 0.19727045057631898 2306 56-57 0.14428077937758843 2306 58-59 0.18885163028173935 2306 60-61 0.17066367678650352 2306 62-63 0.20873986300771463 2306 64-65 0.12311245396040249 2306 66-67 0.23742099128173777 2306 68-69 0.0919516235838671 2306 70-71 0.2386368543331372 2306 72-73 0.18771844131732252 2306 74-75 0.19172835117907283 2306 76-77 0.12871306348981193 2306 78-79 0.10725293152776061 2306 80-81 0.19822112669397995 2306 82-83 0.01133076981451353 2306 84-85 0.17790281167901867 2306 86-87 0.19295977553901622 2306 88-89 0.11099683276566097 2306 90-91 0.16661978251073606 2306 92-93 0.17878043006268385 2306 94-95 0.06588033940525051 2306 96-97 -0.12719767248384883 2306 98-99 0.05047264698932352 2306 100-101 -0.0884819778363628 2307 1 0.058067256980528725 2307 2 0.07638347830086545 2307 3 0.018026728948505877 2307 4 0.09136652863287509 2307 5 0.0836982103924413 2307 6 0.05765067841314675 2307 7 0.23713640019678905 2307 8 0.08912200822526728 2307 9 0.11520950352630877 2307 10-11 0.1486419350225603 2307 12-13 0.07842928417483108 2307 14-15 0.32336160281928983 2307 16-17 0.30104401269476 2307 18-19 0.2587881917362651 2307 20-21 0.33414757341328283 2307 22-23 0.28740318233563045 2307 24-25 0.31389702209636283 2307 26-27 0.2194860610081335 2307 28-29 0.2715397784853124 2307 30-31 0.3294630125975573 2307 32-33 0.2501867680704706 2307 34-35 0.23563795260201204 2307 36-37 0.08534319666615886 2307 38-39 0.0563240434142358 2307 40-41 0.2253153756948052 2307 42-43 0.1759873211164944 2307 44-45 0.24884209996841378 2307 46-47 0.18185246059385207 2307 48-49 0.13592832930155652 2307 50-51 0.08449198297686422 2307 52-53 0.043939203765503976 2307 54-55 0.1879269118680824 2307 56-57 0.0561949484275317 2307 58-59 0.01625736005971845 2307 60-61 0.1032313314789377 2307 62-63 0.19050987838190991 2307 64-65 0.12571862677950207 2307 66-67 0.283317074996674 2307 68-69 0.10727125790963044 2307 70-71 0.08291203576508366 2307 72-73 0.20640303902607116 2307 74-75 0.05329866742447109 2307 76-77 0.01647570586636249 2307 78-79 0.2591350296911159 2307 80-81 0.22454735704229734 2307 82-83 0.17062306122975457 2307 84-85 0.2290302521247014 2307 86-87 0.0920158334649912 2307 88-89 0.10235918418984724 2307 90-91 0.17708662881845072 2307 92-93 0.18036578986827223 2307 94-95 0.20495474716838658 2307 96-97 0.06458374954587143 2307 98-99 0.08328413960806103 2307 100-101 -0.036675924043237274 2201 1 0.14178064150040015 2201 2 0.12473260333557334 2201 3 0.08117741516214849 2201 4 0.20863590063586201 2201 5 0.20221022342098394 2201 6 0.2212194261317464 2201 7 0.3061786337683756 2201 8 0.19004891921871803 2201 9 0.2572118933405889 2201 10-11 0.17487482647944574 2201 12-13 0.1929096555631986 2201 14-15 0.41865622698511373 2201 16-17 0.3031262085784192 2201 18-19 0.32036319165827365 2201 20-21 0.31507882101488605 2201 22-23 0.32984984049546995 2201 24-25 0.4747438462328688 2201 26-27 0.3442633808595019 2201 28-29 0.4938377440233168 2201 30-31 0.37695838740216914 2201 32-33 0.42286707269379775 2201 34-35 0.18619937176482182 2201 36-37 0.2628928258705514 2201 38-39 0.1881541232515076 2201 40-41 0.3092719534558057 2201 42-43 0.36596055270073435 2201 44-45 0.4516768065609966 2201 46-47 0.4025613244337194 2201 48-49 0.41141619771826043 2201 50-51 0.22300543114192095 2201 52-53 0.35801680097259947 2201 54-55 0.3558094927427007 2201 56-57 0.43656056505265184 2201 58-59 0.46689989563395073 2201 60-61 0.4601282543590628 2201 62-63 0.32933874459286727 2201 64-65 0.3864091458309389 2201 66-67 0.42202036676305 2201 68-69 0.2415566691230424 2201 70-71 0.35074657076525995 2201 72-73 0.2294308962736693 2201 74-75 0.29081204383268755 2201 76-77 0.5009798194043853 2201 78-79 0.444160784151741 2201 80-81 0.5812882868235576 2201 82-83 0.5793597667179071 2201 84-85 0.5696784658477299 2201 86-87 0.3544821779539191 2201 88-89 0.4536280320560131 2201 90-91 0.3915106866981297 2201 92-93 0.6225040496716581 2201 94-95 0.3899095247690276 2201 96-97 0.3191432718326084 2201 98-99 0.31075998407670014 2201 100-101 0.23231982358488779 1101 1 0.17061163778752686 1101 2 0.28223068210584756 1101 3 0.22345844969875017 1101 4 0.25733181396935834 1101 5 0.3914997288805466 1101 6 0.2505448490284863 1101 7 0.4346320679172635 1101 8 0.32623572258140854 1101 9 0.40456182216825454 1101 10-11 0.4101562950257076 1101 12-13 0.4802245629924755 1101 14-15 0.6129557421026348 1101 16-17 0.5246573491365467 1101 18-19 0.6117506245246744 1101 20-21 0.46206683189927844 1101 22-23 0.38210480818408143 1101 24-25 0.5581570301446845 1101 26-27 0.5307883731906387 1101 28-29 0.5487614917069621 1101 30-31 0.5596662399416275 1101 32-33 0.40897620963333026 1101 34-35 0.41498229717674917 1101 36-37 0.510735254970399 1101 38-39 0.4452040804780566 1101 40-41 0.5615235062734953 1101 42-43 0.4203597382686084 1101 44-45 0.5076254533235343 1101 46-47 0.4613135948121041 1101 48-49 0.6709053797282536 1101 50-51 0.672572311680689 1101 52-53 0.5726568341756533 1101 54-55 0.6285172898269948 1101 56-57 0.5914591098214643 1101 58-59 0.6120354849808294 1101 60-61 0.6076365952542488 1101 62-63 0.5863051542296844 1101 64-65 0.5449958971241387 1101 66-67 0.6532255885384828 1101 68-69 0.4381926710271955 1101 70-71 0.6202476727178308 1101 72-73 0.40404623441447285 1101 74-75 0.4237869713834357 1101 76-77 0.3740619096906528 1101 78-79 0.46114473221535235 1101 80-81 0.639535039558595 1101 82-83 0.48492136558965626 1101 84-85 0.5292131210960775 1101 86-87 0.5151781228092958 1101 88-89 0.5973599489281263 1101 90-91 0.7083741511109487 1101 92-93 0.5779766138106659 1101 94-95 0.4975443589040758 1101 96-97 0.5534876939620226 1101 98-99 0.5672986592410467 1101 100-101 0.4937396481072227 2304 1 0.1652789056516859 2304 2 0.05703421838495615 2304 3 0.1344249380059388 2304 4 0.186706944101374 2304 5 0.15997779707122106 2304 6 0.06773212973821785 2304 7 0.19650533378509039 2304 8 0.15137032014872887 2304 9 0.17893101950173929 2304 10-11 0.17997443459455553 2304 12-13 0.19999749059169858 2304 14-15 0.3448495766341537 2304 16-17 0.17544836179509105 2304 18-19 0.22804894914363416 2304 20-21 0.2448972912208589 2304 22-23 0.15617485456876068 2304 24-25 0.3280784609440346 2304 26-27 0.3472154928800606 2304 28-29 0.2219036543728734 2304 30-31 0.2070167452271221 2304 32-33 0.16315122488270362 2304 34-35 0.27170025836802125 2304 36-37 0.24949083045728315 2304 38-39 0.22438493894355105 2304 40-41 0.2862331031825178 2304 42-43 0.21607228352469576 2304 44-45 0.2932819661410164 2304 46-47 0.18007254456967559 2304 48-49 0.26127312636966593 2304 50-51 0.20493287055546716 2304 52-53 0.13296445744851582 2304 54-55 0.23099963191288708 2304 56-57 0.32234317416986613 2304 58-59 0.2821323887142242 2304 60-61 0.31821741772390055 2304 62-63 0.3752932503564992 2304 64-65 0.39450335675979886 2304 66-67 0.2895292256656674 2304 68-69 0.34190780277170063 2304 70-71 0.3273474042692257 2304 72-73 0.3403325794223875 2304 74-75 0.3554717667242784 2304 76-77 0.42142792902036064 2304 78-79 0.3280879144376385 2304 80-81 0.4492498580361328 2304 82-83 0.44938884296806947 2304 84-85 0.3379131473464412 2304 86-87 0.3249332533702258 2304 88-89 0.16538631818844962 2304 90-91 0.18957433277320845 2304 92-93 0.1608016574685216 2304 94-95 0.3187929123517925 2304 96-97 0.2554417305860248 2304 98-99 0.4090447533802113 2304 100-101 0.14583394906447822 2202 1 0.22811710306663358 2202 2 0.20552748290504397 2202 3 0.18987727708823243 2202 4 0.27055812182638306 2202 5 0.35916172882657804 2202 6 0.36984143967729466 2202 7 0.42364088941027234 2202 8 0.28682939483830694 2202 9 0.33102926052490034 2202 10-11 0.32480081820267515 2202 12-13 0.3196143863683716 2202 14-15 0.5103189152275789 2202 16-17 0.4639423319744793 2202 18-19 0.4669198483905177 2202 20-21 0.5215910837371425 2202 22-23 0.3857660791220425 2202 24-25 0.35694030890018524 2202 26-27 0.3929555269707947 2202 28-29 0.474856278139022 2202 30-31 0.5235336694594395 2202 32-33 0.47793565360563406 2202 34-35 0.44012075591133026 2202 36-37 0.4514410995631408 2202 38-39 0.40981870415566846 2202 40-41 0.48213482751327064 2202 42-43 0.4480816649722712 2202 44-45 0.506266059255438 2202 46-47 0.3726730188644467 2202 48-49 0.4714398683151444 2202 50-51 0.3948707547035255 2202 52-53 0.5049976631772424 2202 54-55 0.5087357367295269 2202 56-57 0.47537259252062114 2202 58-59 0.5672321248689727 2202 60-61 0.6100093261807871 2202 62-63 0.637937098497595 2202 64-65 0.5872909454098476 2202 66-67 0.5557750569896527 2202 68-69 0.4607941373607005 2202 70-71 0.6230901132418225 2202 72-73 0.469267426178412 2202 74-75 0.5190058282878312 2202 76-77 0.5971325120100985 2202 78-79 0.5864225915806713 2202 80-81 0.7668269271145789 2202 82-83 0.68336056870249 2202 84-85 0.595750360150106 2202 86-87 0.5714494108030692 2202 88-89 0.5074482368633788 2202 90-91 0.30125612780745215 2202 92-93 0.43646033439042853 2202 94-95 0.5720467150784891 2202 96-97 0.608588423400299 2202 98-99 0.6170386633110816 2202 100-101 0.42560653060824905 1102 1 0.060435269295410876 1102 2 -0.08750883292677258 1102 3 -0.10515254838275112 1102 4 -0.08433788096984784 1102 5 0.04078743514735095 1102 6 -0.017767177612370233 1102 7 -0.07960137420941038 1102 8 0.011719595637948999 1102 9 0.05127677024816535 1102 10-11 0.03555488087639702 1102 12-13 -0.017479367528295597 1102 14-15 -0.04802359994158678 1102 16-17 -0.07262932730174043 1102 18-19 0.056348194947879904 1102 20-21 -0.01511790809363589 1102 22-23 -0.10683819821582574 1102 24-25 -0.11249262432143325 1102 26-27 -0.04518077546806154 1102 28-29 -0.06169642195268921 1102 30-31 -0.06119990903073358 1102 32-33 -0.004651003843953561 1102 34-35 -0.09107202472288378 1102 36-37 -0.1411562260737398 1102 38-39 -0.10469844065919887 1102 40-41 -0.0665347360195554 1102 42-43 -0.07430864581493779 1102 44-45 0.03277239684899769 1102 46-47 0.016176180308470123 1102 48-49 -0.040762129156483695 1102 50-51 0.047841780451314264 1102 52-53 0.04009135679341824 1102 54-55 0.10404714255063396 1102 56-57 0.1396572077986562 1102 58-59 0.19743037474178493 1102 60-61 0.2121250131802057 1102 62-63 0.0984744066482719 1102 64-65 0.0513592193778436 1102 66-67 0.22365869669611982 1102 68-69 0.2784688857443669 1102 70-71 0.2724074246058379 1102 72-73 0.1345510446408653 1102 74-75 0.22584725002249684 1102 76-77 0.278035581720836 1102 78-79 0.11025106589377032 1102 80-81 0.148064407672301 1102 82-83 0.2829342058105695 1102 84-85 0.1859583424043052 1102 86-87 0.07954480029399491 1102 88-89 0.03564425101081525 1102 90-91 -0.08118009580494245 1102 92-93 0.09104690141928984 1102 94-95 0.048152491285733845 1102 96-97 -0.009415181761557534 1102 98-99 0.04331143833531925 1102 100-101 -0.014883870575385316 2305 1 0.1637830774866309 2305 2 0.08357461743613825 2305 3 0.11865087761867343 2305 4 0.1654298260051874 2305 5 0.12337577621263307 2305 6 0.24549192978007284 2305 7 0.22023130788545586 2305 8 0.29515757702550616 2305 9 0.12990257713876474 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-0.26324795577853166 1213 64-65 -0.29740828079488324 1213 66-67 -0.27041186887694835 1213 68-69 -0.17925654899141819 1213 70-71 -0.1727169104384032 1213 72-73 -0.30572505677784534 1213 74-75 -0.30013741281597106 1213 76-77 -0.19052063625054672 1213 78-79 -0.09109119989346226 1213 80-81 -0.13730628103696318 1213 82-83 -0.03677774490243735 1213 84-85 -0.0023364448301634866 1213 86-87 -0.0831308313377832 1213 88-89 -0.12569118742311858 1213 90-91 -0.16905418803110805 1213 92-93 -0.07943873472342133 1213 94-95 0.05351931928926135 1213 96-97 0.07414759940957438 1213 98-99 -0.1737366660581614 1213 100-101 0.1445400308506386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 396.0 3 240.0 4 164.0 5 173.0 6 440.0 7 2422.0 8 9315.0 9 24753.0 10 28336.0 11 21412.0 12 13681.0 13 9733.0 14 7563.0 15 6679.0 16 6585.0 17 6974.0 18 7719.0 19 8457.0 20 9462.0 21 11010.0 22 12610.0 23 13578.0 24 16086.0 25 18609.0 26 22191.0 27 26573.0 28 32564.0 29 40799.0 30 51396.0 31 68567.0 32 93940.0 33 135677.0 34 209821.0 35 359887.0 36 681584.0 37 937198.0 38 208703.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.54997463609574 25.068863322761814 19.017506902467073 16.36365513867537 2 33.79207655650592 23.184154403580038 28.58527913377062 14.43848990614343 3 39.738207060280764 19.59531120974401 23.08000232246078 17.586479407514453 4 33.41053254880612 18.50913812521044 29.072067795123324 19.00826153086012 5 35.91334165292343 16.945127798386807 27.970853265460615 19.170677283229146 6 32.04543446676602 17.37415659981802 31.53728243128824 19.043126502127723 7 36.39076393962039 17.951172238947052 24.605637553427933 21.052426268004623 8 31.633182676870902 25.671796723383128 22.46017060071309 20.234849999032875 9 31.36332193761213 23.608096355840473 24.095020071984514 20.93356163456288 10-11 25.35565151573994 28.497225804569855 28.491231202612227 17.65589147707798 12-13 26.113387699587893 25.951120661266792 28.072052561174125 19.86343907797119 14-15 25.526336997517813 25.99987105509171 28.200799458431387 20.272992488959094 16-17 25.677587031072065 26.075960016361687 28.728126796006535 19.518326156559713 18-19 25.191540054397198 26.08649757239973 28.623604554899124 20.098357818303946 20-21 24.35717035857796 27.121675857564163 28.13301033977983 20.388143444078047 22-23 25.21754446125356 26.520651898566918 27.675426242114415 20.58637739806511 24-25 24.048627877474328 26.95930445330412 28.234600557875368 20.75746711134618 26-27 23.155524003509438 27.48973630974302 28.71200708598304 20.642732600764507 28-29 23.372906655286638 27.984848401826778 27.75485727528444 20.887387667602145 30-31 23.696192927542814 27.897127719527564 27.85398173707177 20.552697615857852 32-33 23.546537002058265 27.548076868778477 28.254166425322996 20.65121970384026 34-35 22.809543547108586 27.431510446372204 28.11056941211093 21.648376594408283 36-37 21.36868655413074 27.919716096828356 28.830228962602654 21.881368386438247 38-39 21.318701638506283 28.183314949861206 28.91020970066458 21.58777371096793 40-41 22.043400239373486 27.412491372002467 29.296694367688385 21.247414020935658 42-43 22.998176478182287 27.113671572424476 28.718403046733798 21.16974890265944 44-45 22.03912966173698 27.69515379097438 28.759939967324723 21.505776579963918 46-47 22.669410256704044 28.073874889518645 28.376388450262695 20.88032640351462 48-49 21.613618298951856 28.220619334543084 28.762086066053875 21.40367630045119 50-51 21.463028306229905 27.742796852830164 28.025199175045064 22.768975665894864 52-53 23.194017944617194 27.032580929552342 27.563871710573025 22.209529415257443 54-55 23.923353201451363 26.89987668732202 27.984589464283527 21.19218064694309 56-57 24.500884104827726 27.036619363831722 26.97077936744558 21.491717163894965 58-59 23.756353383131145 27.519646969202725 26.93385964012257 21.79014000754356 60-61 22.487667920399193 27.276690688072218 27.017476286261342 23.21816510526724 62-63 23.22319931451762 27.07894094713783 27.515189263227395 22.182670475117153 64-65 22.62564786727962 27.804812120101722 28.237836481330714 21.33170353128794 66-67 22.468434519603207 27.685697063330007 27.743088112681306 22.10278030438548 68-69 22.66564861068907 26.890451886532563 29.220254926894164 21.2236445758842 70-71 22.182234452763296 28.25954393729416 28.405628765518692 21.15259284442385 72-73 22.098281642950816 29.646195181736058 27.0535232020861 21.20199997322703 74-75 22.474028496688643 29.190648952552444 26.85343539542691 21.481887155331997 76-77 22.128404641647737 28.779161099652722 26.69499042935951 22.39744382934003 78-79 21.851631434898238 28.887211569632136 27.110430506566548 22.15072648890308 80-81 21.391053128521158 28.91530062312682 27.602950441143598 22.090695807208427 82-83 20.856019075705902 28.9400109059347 27.719933660941585 22.48403635741781 84-85 20.882297947813775 29.452220651439703 27.426443577905946 22.239037822840576 86-87 20.688052406323244 29.353313534471187 27.731184627616184 22.22744943158938 88-89 21.082683460371438 29.136520596242775 27.673874832644334 22.10692111074145 90-91 21.596467851577497 28.984991272870403 26.927937461486096 22.490603414066005 92-93 21.61023106637745 28.950915390065536 26.854000957702855 22.584852585854158 94-95 21.936614947904907 29.206573981484468 26.73484726299152 22.121963807619107 96-97 21.896365590430054 29.895837819467168 26.57952639008049 21.628270200022293 98-99 22.182698239392085 29.442124628635884 26.7641892711584 21.610987860813633 100-101 22.289316996104567 29.56515874716362 26.420563817676317 21.724960439055494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 140.0 2 181.5 3 168.5 4 133.0 5 124.5 6 157.0 7 194.5 8 268.0 9 391.5 10 503.0 11 612.5 12 712.5 13 742.0 14 728.5 15 677.0 16 603.0 17 566.5 18 559.5 19 584.0 20 651.5 21 783.5 22 957.5 23 1215.0 24 1580.5 25 2045.0 26 2650.0 27 3454.0 28 4448.0 29 5582.0 30 7271.5 31 9552.0 32 13985.5 33 22509.0 34 36156.5 35 55241.0 36 83352.5 37 116187.5 38 137626.0 39 141945.0 40 143275.5 41 146574.0 42 155875.0 43 171612.0 44 172415.5 45 164780.0 46 163303.0 47 160439.5 48 152359.5 49 137287.5 50 123599.5 51 120134.5 52 115749.5 53 105073.5 54 91038.0 55 75541.0 56 61367.5 57 47179.0 58 34155.0 59 25193.5 60 19069.5 61 15013.5 62 11784.0 63 9120.0 64 7122.5 65 5181.5 66 3726.0 67 2839.0 68 2268.0 69 1662.5 70 1159.5 71 920.0 72 719.0 73 520.0 74 396.5 75 317.0 76 257.0 77 192.5 78 159.0 79 132.5 80 100.5 81 83.5 82 68.5 83 52.5 84 45.5 85 43.5 86 30.0 87 22.5 88 21.5 89 16.5 90 13.0 91 12.0 92 9.0 93 5.0 94 3.5 95 4.5 96 4.0 97 2.0 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20777086210094597 2 0.1910899404381608 3 0.1669380268337421 4 0.15160961233280434 5 0.16201103645844067 6 0.12591197625770287 7 0.11277333525689909 8 0.10832938315368604 9 0.0969296799324004 10-11 0.08219701263370499 12-13 0.10815226912058695 14-15 0.10446507697697904 16-17 0.09580259063086086 18-19 0.0899900300900641 20-21 0.09366112095793575 22-23 0.08147245522557243 24-25 0.3922270769357598 26-27 0.18366725232373612 28-29 0.09122982832175759 30-31 0.17399038560623237 32-33 0.20527516436182272 34-35 0.20447010057500878 36-37 0.21063688918200366 38-39 0.21079790193936646 40-41 0.20677258300529666 42-43 0.22361451742544466 44-45 0.2049692401228334 46-47 0.22446788503946744 48-49 0.21353511881453394 50-51 0.21738332371550464 52-53 0.20854372333628737 54-55 0.19018826899692917 56-57 0.1984965272768492 58-59 0.19356953690154777 60-61 0.17390987922755097 62-63 0.16247797345479278 64-65 0.1665515962160714 66-67 0.17896567980874262 68-69 0.1712531687310649 70-71 0.1639270882710579 72-73 0.16753377403598443 74-75 0.15710014735887554 76-77 0.16867696461326023 78-79 0.16613296304692815 80-81 0.17173620700315329 82-83 0.19688639970322128 84-85 0.1944873096185157 86-87 0.19888295789451993 88-89 0.20625734218173575 90-91 0.1872256342614538 92-93 0.1680168123080728 94-95 0.16887017992209558 96-97 0.1775809700954226 98-99 0.1919272067764473 100-101 0.20377774571834875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3105344.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.69344550316098 #Duplication Level Percentage of deduplicated Percentage of total 1 86.48150492684984 43.84045457040611 2 6.682972599992608 6.775658145936866 3 2.0769169495458613 3.1585822858918338 4 1.0572370559531397 2.1437995631953135 5 0.684979170154523 1.7361977116514375 6 0.45960575625356465 1.39793996145475 7 0.3878657066610737 1.3763574344217715 8 0.27595019863623843 1.1191093084922081 9 0.17581938075353906 0.802160117696614 >10 1.3635583693695301 13.709563296203203 >50 0.2055651375991563 7.283069832861805 >100 0.14060500016157942 13.378972587370294 >500 0.00562221169797003 1.8925261403759017 >1k 0.0017975363713879408 1.3856090440422353 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4834 0.1556671338183467 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4308 0.13872859174378105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001191494404484656 0.0 0.0 2 0.0012881020589023309 0.0 0.0 3 0.0013847097133200057 0.0 0.0 4 0.0015135199192102388 0.0 0.0 5 0.0018677479854083798 0.0 0.0 6 0.0019643556398260546 0.0 0.0 7 0.0021897735001339628 0.0 0.0 8 0.002447393911914429 0.0 0.0 9 0.0029948372869479196 0.0 0.0 10-11 0.004830382720883741 0.0 0.0 12-13 0.006682029430555842 0.0 0.0 14-15 0.008742992724799573 0.0 0.0 16-17 0.010562436882999114 0.0 0.0 18-19 0.012607298901506563 0.0 0.0 20-21 0.015167401743574947 0.0 0.0 22-23 0.017824112240061002 0.0 0.0 24-25 0.020802848251272647 0.0 0.0 26-27 0.024715458255188477 0.0 0.0 28-29 0.029239916737082913 0.0 0.0 30-31 0.06229583582366398 0.0 0.0 32-33 0.06981513159250634 0.0 0.0 34-35 0.07630394571422683 0.0 0.0 36-37 0.08340460831392593 0.0 0.0 38-39 0.09187387935120875 0.0 0.0 40-41 0.10192107541064693 0.0 0.0 42-43 0.11417414624595536 0.0 0.0 44-45 0.1284398765482987 0.0 0.0 46-47 0.14336575915582944 0.0 0.0 48-49 0.1601593897487686 0.0 0.0 50-51 0.17946481935656727 0.0 0.0 52-53 0.20199050411162178 0.0 0.0 54-55 0.22725340574184374 0.0 0.0 56-57 0.25396542218833085 0.0 0.0 58-59 0.28325364275262255 0.0 0.0 60-61 0.31387826920302553 0.0 0.0 62-63 0.349381582201521 0.0 0.0 64-65 0.3864628202221718 0.0 0.0 66-67 0.4274727695224748 0.0 0.0 68-69 0.4716063663156159 0.0 0.0 70-71 0.5205059407266956 0.0 0.0 72-73 0.5757172152263967 0.0 0.0 74-75 0.6388664186640836 0.0 0.0 76-77 0.7123848436759341 0.0 0.0 78-79 0.7918285381587354 0.0 0.0 80-81 0.8794033768883576 0.0 0.0 82-83 0.9808736165783888 0.0 0.0 84-85 1.099540018754766 0.0 0.0 86-87 1.233245012468828 0.0 0.0 88-89 1.379428494878506 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 900 0.0 67.45698 9 AGAGCGG 1180 0.0 54.262173 8 GGATCGG 780 0.0 51.728973 1 GATCGGG 2505 0.0 47.56369 1 TTGTGTT 27555 0.0 42.654003 1 ATCGGGA 2520 0.0 42.563114 2 TCGGGAG 2205 0.0 41.749916 3 CGGGAGA 2290 0.0 41.236332 4 GAGAGCG 2170 0.0 38.46975 7 CGGAGAG 925 0.0 37.96229 5 AAGGGGC 1760 0.0 37.189972 9 TCGGAAA 945 0.0 36.65671 4 GGCGCCG 1375 0.0 34.369656 56-57 TCGGGAA 790 0.0 34.238144 3 CGCCGGA 740 0.0 34.01531 58-59 GAGGGGC 2430 0.0 33.76753 9 GATCTCG 1935 0.0 32.41433 46-47 GTCGCCG 1080 0.0 32.3235 56-57 TCTCGGG 1165 0.0 31.81154 48-49 GAATCGG 500 0.0 31.329502 1 >>END_MODULE