FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105344
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA48340.1556671338183467No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC43080.13872859174378105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9000.067.456989
AGAGCGG11800.054.2621738
GGATCGG7800.051.7289731
GATCGGG25050.047.563691
TTGTGTT275550.042.6540031
ATCGGGA25200.042.5631142
TCGGGAG22050.041.7499163
CGGGAGA22900.041.2363324
GAGAGCG21700.038.469757
CGGAGAG9250.037.962295
AAGGGGC17600.037.1899729
TCGGAAA9450.036.656714
GGCGCCG13750.034.36965656-57
TCGGGAA7900.034.2381443
CGCCGGA7400.034.0153158-59
GAGGGGC24300.033.767539
GATCTCG19350.032.4143346-47
GTCGCCG10800.032.323556-57
TCTCGGG11650.031.8115448-49
GAATCGG5000.031.3295021
Produced by FastQC (version 0.11.2)