##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8566642 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.654598616353994 33.0 33.0 33.0 27.0 33.0 2 31.41424819666796 33.0 33.0 33.0 27.0 33.0 3 31.333533839747243 33.0 33.0 33.0 27.0 33.0 4 34.90796767274738 37.0 37.0 37.0 33.0 37.0 5 34.89557752033994 37.0 37.0 37.0 33.0 37.0 6 35.20572203204009 37.0 37.0 37.0 33.0 37.0 7 34.87116795589217 37.0 37.0 37.0 33.0 37.0 8 34.97273855963632 37.0 37.0 37.0 33.0 37.0 9 34.688468013487665 37.0 37.0 37.0 33.0 37.0 10-11 34.662511985443075 37.0 37.0 37.0 33.0 37.0 12-13 34.6668765894501 37.0 37.0 37.0 33.0 37.0 14-15 36.323901594113536 40.0 37.0 40.0 33.0 40.0 16-17 36.261660753420074 40.0 37.0 40.0 33.0 40.0 18-19 36.23537355710674 40.0 37.0 40.0 33.0 40.0 20-21 36.17329549898315 40.0 37.0 40.0 30.0 40.0 22-23 36.56894912849165 40.0 37.0 40.0 33.0 40.0 24-25 36.1431594199921 40.0 37.0 40.0 27.0 40.0 26-27 36.014878700428945 40.0 37.0 40.0 27.0 40.0 28-29 35.8459647315716 40.0 37.0 40.0 27.0 40.0 30-31 35.80603893567631 40.0 37.0 40.0 27.0 40.0 32-33 35.663041714594826 40.0 37.0 40.0 27.0 40.0 34-35 35.784727959917085 40.0 37.0 40.0 27.0 40.0 36-37 35.546767099640675 38.5 37.0 40.0 27.0 40.0 38-39 35.48102868078297 37.0 37.0 40.0 27.0 40.0 40-41 35.67345098581218 37.0 37.0 40.0 27.0 40.0 42-43 35.44820864464746 37.0 37.0 40.0 27.0 40.0 44-45 35.13506062235354 37.0 37.0 40.0 22.0 40.0 46-47 34.98575602902514 37.0 37.0 40.0 22.0 40.0 48-49 34.85101852044244 37.0 37.0 40.0 22.0 40.0 50-51 33.78375920226385 37.0 33.0 38.5 22.0 38.5 52-53 34.20343916554468 37.0 33.0 38.5 22.0 40.0 54-55 34.90793755592915 37.0 37.0 40.0 24.5 40.0 56-57 35.060833754929874 37.0 37.0 40.0 24.5 40.0 58-59 34.69325051753067 37.0 33.0 40.0 22.0 40.0 60-61 34.45920087474182 37.0 33.0 40.0 22.0 40.0 62-63 34.15459067858795 37.0 33.0 40.0 22.0 40.0 64-65 33.90881000980315 37.0 33.0 38.5 22.0 40.0 66-67 33.69245271367707 37.0 33.0 37.0 22.0 40.0 68-69 33.59628282587273 37.0 33.0 37.0 22.0 40.0 70-71 33.230583232029545 37.0 33.0 37.0 22.0 40.0 72-73 33.22642109942262 37.0 33.0 37.0 22.0 38.5 74-75 33.10863223886325 37.0 33.0 37.0 22.0 37.0 76-77 32.845943369642384 37.0 33.0 37.0 22.0 37.0 78-79 32.4944127465581 37.0 33.0 37.0 22.0 37.0 80-81 32.127009042749776 37.0 33.0 37.0 22.0 37.0 82-83 31.841255126571184 35.0 33.0 37.0 18.5 37.0 84-85 31.618774544331373 33.0 33.0 37.0 15.0 37.0 86-87 31.443118610536075 33.0 33.0 37.0 15.0 37.0 88-89 31.248949705147012 33.0 33.0 37.0 15.0 37.0 90-91 31.104988454052357 33.0 33.0 37.0 15.0 37.0 92-93 30.921077068470936 33.0 33.0 37.0 15.0 37.0 94-95 30.777156790256903 33.0 30.0 37.0 15.0 37.0 96-97 30.63352338057316 33.0 27.0 37.0 15.0 37.0 98-99 30.480391558325888 33.0 27.0 37.0 15.0 37.0 100-101 29.149932085407563 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18271718485606314 2306 2 0.2232508579564545 2306 3 0.15725278690092992 2306 4 0.3439602072565364 2306 5 0.3668956916099546 2306 6 0.15153861925865897 2306 7 0.49201215140870147 2306 8 0.19687414010649462 2306 9 0.3760613589496913 2306 10-11 0.2989417185096812 2306 12-13 0.300893759106188 2306 14-15 0.4785824952191362 2306 16-17 0.49178826982269896 2306 18-19 0.4352752064598562 2306 20-21 0.47451489706865146 2306 22-23 0.2888368915072306 2306 24-25 0.3842371774372637 2306 26-27 0.3176133500852174 2306 28-29 0.3780028121014922 2306 30-31 0.4499528399349231 2306 32-33 0.4444964832485212 2306 34-35 0.3303971369418264 2306 36-37 0.46837764557849226 2306 38-39 0.3458224026521819 2306 40-41 0.17451832139901313 2306 42-43 0.4155600039214846 2306 44-45 0.5893137301440987 2306 46-47 0.5434872512736035 2306 48-49 0.5562384997732792 2306 50-51 0.47037271059421215 2306 52-53 0.4607163850099525 2306 54-55 0.3804233040007645 2306 56-57 0.25306125990212536 2306 58-59 0.49238708360190486 2306 60-61 0.5042263515251477 2306 62-63 0.5928783577233716 2306 64-65 0.5267313168925014 2306 66-67 0.4737661048673516 2306 68-69 0.36423824271465577 2306 70-71 0.4217517499112944 2306 72-73 0.18688827418107934 2306 74-75 0.0994935215453907 2306 76-77 0.11775784967249336 2306 78-79 0.13211683316090017 2306 80-81 0.4049834184764407 2306 82-83 0.49322778210081 2306 84-85 0.42511956966817976 2306 86-87 0.3649199803798133 2306 88-89 0.2887505697620334 2306 90-91 0.21420226869346948 2306 92-93 0.2839237565135697 2306 94-95 0.10871169566744499 2306 96-97 0.08915052635696341 2306 98-99 0.2785602236130096 2306 100-101 0.23359624095511577 2307 1 0.052490115155649164 2307 2 0.05742764774281284 2307 3 0.01879192738249813 2307 4 0.1493044764872451 2307 5 0.19313204418823915 2307 6 0.02818410884042777 2307 7 0.2661905754159619 2307 8 0.0516750282672902 2307 9 0.18653164663336952 2307 10-11 0.09022361647852506 2307 12-13 0.08979187229909513 2307 14-15 0.38622921555057843 2307 16-17 0.32595101306636565 2307 18-19 0.32224993651989564 2307 20-21 0.33139147638748767 2307 22-23 0.1470104829796739 2307 24-25 0.3214750233517236 2307 26-27 0.11269692124888309 2307 28-29 0.21763879332692682 2307 30-31 0.2815488133571833 2307 32-33 0.27772072875525566 2307 34-35 0.16094849047297544 2307 36-37 0.15803153614586307 2307 38-39 0.1021310954494723 2307 40-41 0.076342920123885 2307 42-43 0.12515653899580315 2307 44-45 0.24300586389151135 2307 46-47 0.27774144379971233 2307 48-49 0.19523587956894772 2307 50-51 -0.02014570274606342 2307 52-53 0.07653567215000834 2307 54-55 0.2098924972071856 2307 56-57 0.08501524982257536 2307 58-59 0.1901135563242562 2307 60-61 0.1473242473320937 2307 62-63 0.1390414554849997 2307 64-65 0.15484746115007653 2307 66-67 0.14947775356038306 2307 68-69 0.034405280741168554 2307 70-71 0.11381864520815554 2307 72-73 -0.08343489798110681 2307 74-75 -0.18110131599376444 2307 76-77 -0.032628852172031486 2307 78-79 0.04827265292656335 2307 80-81 0.22286269399770475 2307 82-83 0.2068697083682025 2307 84-85 0.35708814073855066 2307 86-87 0.27134331497873276 2307 88-89 0.2533646163676231 2307 90-91 0.23679452093499265 2307 92-93 0.26313786083577284 2307 94-95 0.2250370514380684 2307 96-97 0.17233212121400143 2307 98-99 0.12964380955699184 2307 100-101 -0.033474475211008325 2201 1 0.14902511819405717 2201 2 0.15114137747995215 2201 3 0.16003340123848986 2201 4 0.26156253455112477 2201 5 0.24353184331406652 2201 6 0.09868355953869212 2201 7 0.40110084253986855 2201 8 0.20953318894322592 2201 9 0.32550088190117776 2201 10-11 0.284682230335072 2201 12-13 0.2668209036565088 2201 14-15 0.40588911502901937 2201 16-17 0.30996342953326206 2201 18-19 0.2902926474298866 2201 20-21 0.3345026205007642 2201 22-23 0.2752966261426266 2201 24-25 0.4088375929332173 2201 26-27 0.23144832581697727 2201 28-29 0.29562183379076856 2201 30-31 0.321461110050123 2201 32-33 0.26672406204504995 2201 34-35 0.14989418458485204 2201 36-37 0.27346902175427346 2201 38-39 0.21827139306814303 2201 40-41 0.08134263635804473 2201 42-43 0.31266787809919094 2201 44-45 0.37652389423712407 2201 46-47 0.2681488475587983 2201 48-49 0.27043334199836977 2201 50-51 0.11872261237763126 2201 52-53 0.2434088842613633 2201 54-55 0.2847970788190892 2201 56-57 0.34509776087014643 2201 58-59 0.4249802196346053 2201 60-61 0.4009063844835765 2201 62-63 0.39147127028858364 2201 64-65 0.3325530033809585 2201 66-67 0.27386704407732765 2201 68-69 0.21586530721270947 2201 70-71 0.41036738503779446 2201 72-73 0.19856248001281074 2201 74-75 0.18213688724851096 2201 76-77 0.3242194492308883 2201 78-79 0.3417823674037521 2201 80-81 0.4682471175784926 2201 82-83 0.4564444237199865 2201 84-85 0.39231978178044 2201 86-87 0.4208579467368452 2201 88-89 0.3110659242004452 2201 90-91 0.3241438489197108 2201 92-93 0.38704056211666327 2201 94-95 0.32047504097965884 2201 96-97 0.3057385264765813 2201 98-99 0.2881063866198055 2201 100-101 0.15547076740004684 1101 1 0.20010753818402804 1101 2 0.17929323324192836 1101 3 0.11743956138749923 1101 4 0.3134789243448637 1101 5 0.3425989136943173 1101 6 0.228262944930556 1101 7 0.43593140205654635 1101 8 0.1866150112813827 1101 9 0.2970360220363659 1101 10-11 0.28831675850754124 1101 12-13 0.309998239248614 1101 14-15 0.5653810402044215 1101 16-17 0.4580527132292005 1101 18-19 0.5315560263037611 1101 20-21 0.42137696343214515 1101 22-23 0.3765257052876976 1101 24-25 0.4733523149200636 1101 26-27 0.3865665213066265 1101 28-29 0.43671297303855994 1101 30-31 0.4719492513507646 1101 32-33 0.3358547485019159 1101 34-35 0.2438211949743021 1101 36-37 0.4641342068991534 1101 38-39 0.30090964097066575 1101 40-41 0.2917738282089246 1101 42-43 0.2964622792385754 1101 44-45 0.36555953812953845 1101 46-47 0.35711431934208093 1101 48-49 0.3258225268008488 1101 50-51 0.4203578934763712 1101 52-53 0.4459881101761809 1101 54-55 0.4612142181106762 1101 56-57 0.4373264956244469 1101 58-59 0.5724922672291015 1101 60-61 0.5723791715295263 1101 62-63 0.5704051718114087 1101 64-65 0.4960256280744204 1101 66-67 0.4598803879480897 1101 68-69 0.3093980598372781 1101 70-71 0.4576432031207247 1101 72-73 0.23653154102483143 1101 74-75 0.2883293187469249 1101 76-77 0.42635413013414336 1101 78-79 0.49147045818489943 1101 80-81 0.5541473676765349 1101 82-83 0.5678414027692185 1101 84-85 0.49218429265374297 1101 86-87 0.4839364795569274 1101 88-89 0.46773971756642396 1101 90-91 0.5080791693679174 1101 92-93 0.5426656888854247 1101 94-95 0.5678527828069058 1101 96-97 0.42657127089504954 1101 98-99 0.47840004113680124 1101 100-101 0.4222845355991751 2304 1 0.19223944454775577 2304 2 0.14435163989233857 2304 3 0.17920944083162382 2304 4 0.28528265165174815 2304 5 0.3011332608167976 2304 6 0.2625087184388022 2304 7 0.4561122732984373 2304 8 0.27438142659660514 2304 9 0.2501630944881583 2304 10-11 0.2727171004293396 2304 12-13 0.2927370268633851 2304 14-15 0.4908958316156671 2304 16-17 0.45790884858471514 2304 18-19 0.37266748173191644 2304 20-21 0.45729357283739347 2304 22-23 0.2755138622783022 2304 24-25 0.36644920723415453 2304 26-27 0.3246737356469893 2304 28-29 0.3492359337459945 2304 30-31 0.3320577261909179 2304 32-33 0.2937268092693799 2304 34-35 0.23615370344375464 2304 36-37 0.4081370273048748 2304 38-39 0.22916504637404245 2304 40-41 0.3492030673131623 2304 42-43 0.4526048205079718 2304 44-45 0.4637241785652364 2304 46-47 0.4570312341415743 2304 48-49 0.43160585556824316 2304 50-51 0.3478231661081068 2304 52-53 0.2876296641538616 2304 54-55 0.3190898717516788 2304 56-57 0.3827351775039318 2304 58-59 0.5246265089606297 2304 60-61 0.3951972119463605 2304 62-63 0.5141216102557493 2304 64-65 0.45979419312563863 2304 66-67 0.3927631150588624 2304 68-69 0.3319511258308765 2304 70-71 0.5008347826227819 2304 72-73 0.3650721307650855 2304 74-75 0.38014400431809037 2304 76-77 0.45635731880940256 2304 78-79 0.3711476684383612 2304 80-81 0.45121558287168995 2304 82-83 0.5096757742932994 2304 84-85 0.5210700976595142 2304 86-87 0.4654385701602166 2304 88-89 0.3491933043769855 2304 90-91 0.3447993848205684 2304 92-93 0.49581985759179403 2304 94-95 0.41159991037936194 2304 96-97 0.28963039312610306 2304 98-99 0.3900711914102324 2304 100-101 0.33317812815833037 2202 1 0.08084808949940481 2202 2 0.04927388276999167 2202 3 0.04715198524193909 2202 4 0.038757490693605234 2202 5 0.1634127764602411 2202 6 0.11386900706204983 2202 7 0.2396804900741074 2202 8 0.09671099848193876 2202 9 0.10195616789986417 2202 10-11 0.12113162460996563 2202 12-13 0.04065128148013031 2202 14-15 0.19708691456509797 2202 16-17 0.1582068285532543 2202 18-19 0.2327574028614876 2202 20-21 0.2258491268371543 2202 22-23 0.2405646831463102 2202 24-25 0.2762644166405863 2202 26-27 0.17609178992015728 2202 28-29 0.2260288584422554 2202 30-31 0.3231594732632175 2202 32-33 0.149600698216382 2202 34-35 0.1528285563230085 2202 36-37 0.18258006513011793 2202 38-39 0.14356161543121715 2202 40-41 0.23335815636899326 2202 42-43 0.270423693164453 2202 44-45 0.24352241793924634 2202 46-47 0.200532392611926 2202 48-49 0.15488367365934153 2202 50-51 0.15011207507586732 2202 52-53 0.17375143015375016 2202 54-55 0.21361724449725017 2202 56-57 0.38014260535017996 2202 58-59 0.3672594896141632 2202 60-61 0.33808553470991853 2202 62-63 0.3000978913797141 2202 64-65 0.2614703029934944 2202 66-67 0.3134675796059767 2202 68-69 0.35791927473739804 2202 70-71 0.3619721820465287 2202 72-73 0.26237094601101063 2202 74-75 0.27663308462433633 2202 76-77 0.41810852402215204 2202 78-79 0.3717490278895994 2202 80-81 0.33210514642529887 2202 82-83 0.4006613219902704 2202 84-85 0.4374973141760954 2202 86-87 0.3968337066988319 2202 88-89 0.45539152566719565 2202 90-91 0.27710215223630996 2202 92-93 0.42550850055569356 2202 94-95 0.4284425325701413 2202 96-97 0.36304720294853254 2202 98-99 0.3108862819358329 2202 100-101 0.3752035170400383 1102 1 0.0399583596817763 1102 2 -0.04447163616514871 1102 3 0.036057732883023164 1102 4 -0.019801272323000774 1102 5 0.03963262861638839 1102 6 0.08958263803096855 1102 7 0.09812224241884593 1102 8 0.05102665889877045 1102 9 -0.05193462707163832 1102 10-11 0.03565032142746105 1102 12-13 0.07225650417721141 1102 14-15 0.0028948568684157294 1102 16-17 0.038740645494748094 1102 18-19 0.11435587241665957 1102 20-21 0.006683977999770718 1102 22-23 0.09083515106040352 1102 24-25 0.06616251316374644 1102 26-27 0.12033736976666631 1102 28-29 0.10816356065203792 1102 30-31 0.1583704865642943 1102 32-33 0.17862609798095264 1102 34-35 0.1173842147956563 1102 36-37 0.1353281306583014 1102 38-39 0.104098048242939 1102 40-41 0.15903302807139852 1102 42-43 0.2687194452428727 1102 44-45 0.1462401850736228 1102 46-47 0.16362947959653695 1102 48-49 0.16498440004643555 1102 50-51 0.15216494725090257 1102 52-53 0.23285422123576893 1102 54-55 0.3579202632121863 1102 56-57 0.4084322980955335 1102 58-59 0.4090877281251153 1102 60-61 0.2833943325379522 1102 62-63 0.3510213347070419 1102 64-65 0.3157182384022761 1102 66-67 0.39160601199760237 1102 68-69 0.3974065997796856 1102 70-71 0.4082369426828265 1102 72-73 0.35801812170774383 1102 74-75 0.3991610838040742 1102 76-77 0.4155460117413554 1102 78-79 0.2757143718784363 1102 80-81 0.3392777626785559 1102 82-83 0.38545154212714294 1102 84-85 0.36826543247385857 1102 86-87 0.2682615590828554 1102 88-89 0.28914452923379486 1102 90-91 0.27464560395986837 1102 92-93 0.3361201150056665 1102 94-95 0.34841752433915474 1102 96-97 0.22703203684717366 1102 98-99 0.3048105283806102 1102 100-101 0.39302723123974204 2305 1 0.18422243493183998 2305 2 0.13976422080461504 2305 3 0.15311553887469387 2305 4 0.1764559350302477 2305 5 0.2008735995938835 2305 6 0.24833426185373497 2305 7 0.248781358283928 2305 8 0.25166155168634674 2305 9 0.23201691121724366 2305 10-11 0.23032341420579883 2305 12-13 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-0.5409048042858373 1213 66-67 -0.39471833810348045 1213 68-69 -0.32791247298249004 1213 70-71 -0.43644453692758134 1213 72-73 -0.29615882021155215 1213 74-75 -0.23600748598867938 1213 76-77 -0.18134489737020942 1213 78-79 -0.19782677823635453 1213 80-81 -0.3801679138811025 1213 82-83 -0.41017460618101964 1213 84-85 -0.4653513375092686 1213 86-87 -0.37586808955948925 1213 88-89 -0.38919795276632385 1213 90-91 -0.3701478908477469 1213 92-93 -0.3263771190742979 1213 94-95 -0.31797806458333966 1213 96-97 -0.2796353467746364 1213 98-99 -0.3705134443301148 1213 100-101 -0.13482708463818227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1260.0 3 617.0 4 466.0 5 516.0 6 1451.0 7 7339.0 8 29139.0 9 81256.0 10 112089.0 11 89509.0 12 56136.0 13 39084.0 14 29885.0 15 25078.0 16 23475.0 17 23432.0 18 24802.0 19 26960.0 20 29881.0 21 33809.0 22 37736.0 23 41891.0 24 47330.0 25 55464.0 26 66036.0 27 80529.0 28 98307.0 29 119495.0 30 146118.0 31 188364.0 32 254740.0 33 365050.0 34 550422.0 35 903515.0 36 1652421.0 37 2598858.0 38 722951.0 39 1231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.561544500679275 27.874366685489598 17.28236611647313 17.281722697357992 2 32.22855193858221 27.091913813342718 25.339062984680645 15.34047126339444 3 34.33812977570555 22.592209472455266 24.387039829664943 18.68262092217424 4 30.54731571908255 21.044524814271924 26.482653200453036 21.92550626619249 5 33.48773664175961 19.905246243537523 25.808804940843505 20.79821217385936 6 32.0868270955034 19.783703696084594 27.68588482290778 20.443584385504224 7 33.626132003773925 20.781748024541002 23.210476006936855 22.38164396474823 8 29.05485417372473 26.58270185206329 22.500966168388278 21.8614778058237 9 28.800422060736082 24.626636332511882 25.32076966491042 21.25217194184162 10-11 24.67012394503599 29.623900493472693 28.23532470036028 17.47065086113103 12-13 25.194806788408002 26.97761115538966 28.696005815162895 19.131576241039436 14-15 23.948801338747064 26.96446465601411 29.584925306070698 19.501808699168134 16-17 25.276871007793115 26.838216843032804 29.63659214128439 18.248320007889692 18-19 25.07727660590748 26.639610653264384 30.009034250113427 18.274078490714714 20-21 24.425018038203014 28.24502160202612 29.071133324959327 18.258827034811542 22-23 26.04262407584883 27.197233992663687 28.15819582372054 18.601946107766942 24-25 23.960885840795555 28.6637982184195 28.502035598910513 18.873280341874434 26-27 23.029294352945275 29.76726335562131 28.694190449023672 18.509251842409743 28-29 23.5396627214607 29.338686690170118 28.560307110554334 18.561343477814848 30-31 24.63767743094308 28.0833143113205 28.94663693931448 18.33237131842195 32-33 22.545234569853694 29.354089809638268 29.720933099790127 18.379742520717915 34-35 24.85482055416935 28.27291992902481 28.754513883243266 18.117745633562578 36-37 22.976580549690297 28.694032251460794 30.090028016774973 18.239359182073944 38-39 22.77127975952858 29.59822600212169 28.991432348754447 18.63906188959528 40-41 25.255520876328593 27.425801138201045 28.206718346857762 19.1119596386126 42-43 25.065810024884964 27.339110576051233 28.540105506631896 19.054973892431907 44-45 23.43533880465977 28.178356640405696 29.291205277796024 19.09509927713851 46-47 23.670865631090972 28.69399798870429 28.57257263511152 19.062563745093218 48-49 21.77165277646252 28.956804265895258 29.544666896852373 19.72687606078985 50-51 21.338968572083036 28.192523337370982 29.957721670805316 20.510786419740665 52-53 24.17848087665204 27.843761209844665 28.02449907256402 19.953258840939274 54-55 24.910606026404686 27.736704704158665 28.043099748972907 19.309589520463742 56-57 25.090419849995715 27.4098279737608 28.111080017392986 19.388672158850497 58-59 24.343908391214566 27.6720661860726 28.114992862435358 19.869032560277482 60-61 22.13596106717951 27.875837962025834 27.891659653319028 22.096541317475626 62-63 23.322626570031023 27.868540309321045 28.085090063323893 20.723743057324036 64-65 21.97406995469217 28.42853574837901 29.37785687926471 20.219537417664114 66-67 21.443914467702378 28.078919354203062 28.82782806300329 21.649338115091272 68-69 21.01886193435903 27.784999498640484 31.28800283216968 19.908135734830807 70-71 20.72326398537385 30.086369782730593 29.681177271124575 19.509188960770977 72-73 20.689999916394544 31.843406163429254 27.844370140177116 19.622223779999086 74-75 20.909197146210555 31.7872393934765 27.606497836379283 19.69706562393366 76-77 21.046895169756063 31.49412685643973 27.679531675639495 19.77944629816471 78-79 20.914471571789818 31.262705833627802 27.982247554832256 19.840575039750128 80-81 20.569274515772225 31.498557773656703 27.77858788225839 20.15357982831268 82-83 20.486774869400833 31.324723705814495 27.702738306019477 20.485763118765195 84-85 20.7548734474769 30.83444784983844 27.961524603606115 20.44915409907854 86-87 20.832557932256517 30.501328816180994 28.52588369697353 20.140229554588956 88-89 21.136373047636717 30.767082454938983 28.13485305791191 19.961691439512386 90-91 21.377110759572872 30.309479439340436 27.761732315769706 20.551677485316986 92-93 21.215749084795725 30.398176344216782 27.586997390392543 20.799077180594953 94-95 20.83186948873104 30.455463079748966 27.567033851220067 21.145633580299922 96-97 20.82987572732516 30.748827415486673 27.65110283988345 20.770194017304714 98-99 21.240271081922415 30.41846445741305 27.510106332057756 20.831158128606777 100-101 21.515840383036917 30.352016700080164 27.0476600552045 21.08448286167842 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 303.0 1 429.0 2 537.0 3 468.0 4 396.0 5 409.0 6 510.5 7 687.5 8 934.5 9 1192.0 10 1497.5 11 1775.5 12 1979.0 13 2062.5 14 1927.0 15 1780.5 16 1647.5 17 1499.5 18 1497.5 19 1652.5 20 2003.5 21 2635.5 22 3632.5 23 5116.5 24 7202.0 25 9903.0 26 13581.5 27 18759.0 28 25048.0 29 31637.5 30 39442.0 31 48903.5 32 65358.0 33 93907.5 34 136390.5 35 198192.0 36 271303.5 37 353733.5 38 440086.5 39 490168.0 40 527304.5 41 535179.5 42 503382.5 43 497043.0 44 491508.5 45 487198.0 46 508608.0 47 488536.0 48 409841.0 49 334834.5 50 278278.5 51 233865.5 52 195564.0 53 160642.0 54 134468.0 55 112258.5 56 88127.0 57 66023.5 58 50346.5 59 39413.0 60 31082.5 61 24418.0 62 18826.0 63 14497.5 64 11428.5 65 9133.5 66 7274.0 67 5760.0 68 4623.5 69 3680.5 70 2876.5 71 2247.5 72 1755.0 73 1392.0 74 1069.0 75 803.5 76 632.5 77 488.0 78 376.5 79 311.0 80 234.0 81 185.0 82 175.0 83 155.5 84 119.5 85 97.0 86 84.0 87 73.0 88 70.0 89 57.0 90 45.5 91 43.0 92 34.5 93 29.0 94 29.0 95 26.0 96 21.0 97 12.5 98 7.5 99 5.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21664264714225245 2 0.20075544186391822 3 0.17540128325661328 4 0.15970084894407868 5 0.17019504258494753 6 0.13022605590381856 7 0.11700033688812957 8 0.1125878728211124 9 0.10076293604892092 10-11 0.08643410101647764 12-13 0.11325324438677371 14-15 0.10957619099759275 16-17 0.10086215812450199 18-19 0.09456447462144443 20-21 0.09825320119598788 22-23 0.08522592633146103 24-25 0.40139998846689284 26-27 0.19016786274014952 28-29 0.09221816436358611 30-31 0.17680206550011077 32-33 0.20731577203763155 34-35 0.20707063514501947 36-37 0.2123585881142226 38-39 0.21322824042372732 40-41 0.2098430166686083 42-43 0.22543255572019935 44-45 0.20743834048393758 46-47 0.22664073040521596 48-49 0.21562124342303554 50-51 0.2190356501415607 52-53 0.2109753156487688 54-55 0.19341300827091876 56-57 0.20100641534921151 58-59 0.1961912263871888 60-61 0.17616004030517443 62-63 0.1646386063524074 64-65 0.16900437767797463 66-67 0.18131958823539024 68-69 0.17386625938144723 70-71 0.16678063586642233 72-73 0.170060800953279 74-75 0.16031369117560884 76-77 0.17066780659212794 78-79 0.16888764582434984 80-81 0.17431567701790268 82-83 0.19966399903252638 84-85 0.19809979219395416 86-87 0.2019811263269785 88-89 0.20895001798837864 90-91 0.19008615044261218 92-93 0.17030593784589107 94-95 0.1713098317870643 96-97 0.18030402110885457 98-99 0.1947145684388352 100-101 0.20662705410124527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8566642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.72255129160981 #Duplication Level Percentage of deduplicated Percentage of total 1 90.72057935199813 46.92299818738282 2 4.369812861468262 4.520357397240567 3 1.341200650676808 2.08110958340915 4 0.736532632137303 1.5238138737466413 5 0.5040195472074847 1.3034588441206538 6 0.34143507985032256 1.059593605818792 7 0.2316706692784023 0.8387818652159614 8 0.1690115648620541 0.6993367465962265 9 0.1327721675963144 0.6180583723738727 >10 1.1616556102396602 12.478172101909518 >50 0.171689497447599 6.262168170148354 >100 0.10019292989500586 9.744287020216545 >500 0.010778062642744396 3.956871202873032 >1k 0.008535884726667223 7.524459253020136 >5k 9.086830475388668E-5 0.2570479507442766 >10k+ 2.2621668489596295E-5 0.20948582518348405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 17904 0.20899671072982856 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0032101259746817948 0.0 0.0 2 0.003501955608743776 0.0 0.0 3 0.0037937852428057573 0.0 1.1673185362479253E-5 4 0.004050595320780301 0.0 2.3346370724958507E-5 5 0.004634254588904264 0.0 3.501955608743776E-5 6 0.004937757408328724 0.0 3.501955608743776E-5 7 0.0057198608276148345 0.0 3.501955608743776E-5 8 0.006280173725013839 0.0 4.669274144991701E-5 9 0.006968891661400115 0.0 4.669274144991701E-5 10-11 0.010453337492100173 0.0 4.669274144991701E-5 12-13 0.014048678583743783 0.0 4.669274144991701E-5 14-15 0.018093437311842844 0.0 4.669274144991701E-5 16-17 0.022132359447260663 0.0 5.252933413115664E-5 18-19 0.027291907377476495 0.0 6.42025194936359E-5 20-21 0.03367713977075265 0.0 8.75488902185944E-5 22-23 0.04127638344172664 0.0 9.338548289983403E-5 24-25 0.04957018164176815 0.0 9.922207558107365E-5 26-27 0.059393167124294446 0.0 1.0505866826231328E-4 28-29 0.07028424906748759 0.0 1.1089526094355291E-4 30-31 0.10472598247948263 0.0 1.1673185362479253E-4 32-33 0.11886804654612623 0.0 1.1673185362479253E-4 34-35 0.13274746394211406 0.0 1.1673185362479253E-4 36-37 0.147140501494051 0.0 1.2256844630603215E-4 38-39 0.16262498187737973 0.0 1.284050389872718E-4 40-41 0.17958028361638084 0.0 1.284050389872718E-4 42-43 0.19806477263786673 0.0 1.284050389872718E-4 44-45 0.21622824906188445 0.0 1.284050389872718E-4 46-47 0.2357808345440372 0.0 1.284050389872718E-4 48-49 0.2557069619577893 0.0 1.284050389872718E-4 50-51 0.27744243310272565 0.0 1.284050389872718E-4 52-53 0.29970319758897357 0.0 1.284050389872718E-4 54-55 0.32314295379683194 0.0 1.3424163166851142E-4 56-57 0.34741150616542626 0.0 1.459148170309907E-4 58-59 0.37279484773613747 0.0 1.5758800239346993E-4 60-61 0.39940971036259015 0.0 1.6342459507470955E-4 62-63 0.4277288580519648 0.0 1.6926118775594917E-4 64-65 0.4576647419140428 0.0 1.750977804371888E-4 66-67 0.4893340938024491 0.0 1.8093437311842843E-4 68-69 0.5223458620075404 0.0 1.8677096579966805E-4 70-71 0.5584101681849201 0.0 1.8677096579966805E-4 72-73 0.5960328446081906 0.0 1.8677096579966805E-4 74-75 0.6369940520451305 0.0 1.8677096579966805E-4 76-77 0.6819591620614005 0.0 1.8677096579966805E-4 78-79 0.7294690264866911 0.0 1.8677096579966805E-4 80-81 0.7821676218056037 0.0 1.8677096579966805E-4 82-83 0.8390627272623276 1.1673185362479253E-5 1.8677096579966805E-4 84-85 0.9017710790295661 1.1673185362479253E-5 1.8677096579966805E-4 86-87 0.9696214689489767 1.750977804371888E-5 1.8677096579966805E-4 88-89 1.043722849629995 2.3346370724958507E-5 1.8677096579966805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4695 0.0 83.77743 9 CGGGAGA 6910 0.0 81.13931 4 AGAGCGG 5325 0.0 75.38048 8 ATTCGGA 1080 0.0 75.188896 2 GATCGGG 9205 0.0 74.669014 1 CGGAAAG 2655 0.0 72.955 5 AAGCGGC 1690 0.0 72.71452 9 TTCGGAG 1150 0.0 71.42093 3 ATCGGGA 9395 0.0 71.01677 2 GAGAGCG 7205 0.0 69.08159 7 TCGGGAA 2075 0.0 67.72528 3 GATCCGG 1460 0.0 67.02087 1 GTTCGGA 1185 0.0 65.32067 2 AAAGCGG 2105 0.0 61.534496 8 TCGGAAA 2290 0.0 59.083855 4 GAACCGG 665 0.0 55.714554 1 AAGCGTC 660 0.0 53.916965 9 AAGGGGC 4735 0.0 53.409107 9 ATCCGGA 1780 0.0 53.356995 2 TCGGAGA 2100 0.0 53.352177 4 >>END_MODULE