FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8566642
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT179040.20899671072982856Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC46950.083.777439
CGGGAGA69100.081.139314
AGAGCGG53250.075.380488
ATTCGGA10800.075.1888962
GATCGGG92050.074.6690141
CGGAAAG26550.072.9555
AAGCGGC16900.072.714529
TTCGGAG11500.071.420933
ATCGGGA93950.071.016772
GAGAGCG72050.069.081597
TCGGGAA20750.067.725283
GATCCGG14600.067.020871
GTTCGGA11850.065.320672
AAAGCGG21050.061.5344968
TCGGAAA22900.059.0838554
GAACCGG6650.055.7145541
AAGCGTC6600.053.9169659
AAGGGGC47350.053.4091079
ATCCGGA17800.053.3569952
TCGGAGA21000.053.3521774
Produced by FastQC (version 0.11.2)