##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2681835 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.897775217341856 33.0 33.0 33.0 22.0 33.0 2 30.316038831620887 33.0 33.0 33.0 22.0 33.0 3 30.156926507410038 33.0 33.0 33.0 22.0 33.0 4 33.452708686403156 37.0 37.0 37.0 15.0 37.0 5 33.51862213745439 37.0 37.0 37.0 15.0 37.0 6 34.25913003596418 37.0 37.0 37.0 27.0 37.0 7 33.559462830487334 37.0 37.0 37.0 15.0 37.0 8 33.85223028262365 37.0 37.0 37.0 22.0 37.0 9 33.21709911310726 37.0 37.0 37.0 15.0 37.0 10-11 33.26186118832814 37.0 37.0 37.0 15.0 37.0 12-13 33.24464666916496 37.0 37.0 37.0 15.0 37.0 14-15 34.723045228360434 40.0 37.0 40.0 15.0 40.0 16-17 34.58888745951932 40.0 37.0 40.0 15.0 40.0 18-19 34.70336318229869 40.0 37.0 40.0 15.0 40.0 20-21 34.6329414374859 40.0 37.0 40.0 15.0 40.0 22-23 35.588130701553226 40.0 37.0 40.0 24.5 40.0 24-25 34.80912453599868 38.5 37.0 40.0 15.0 40.0 26-27 34.58731521514187 37.0 37.0 40.0 18.5 40.0 28-29 34.29720471244502 37.0 37.0 40.0 15.0 40.0 30-31 34.303017709888934 37.0 37.0 40.0 15.0 40.0 32-33 34.0988541427791 37.0 37.0 40.0 15.0 40.0 34-35 34.414338130421896 37.0 37.0 40.0 18.5 40.0 36-37 33.953180937678866 37.0 35.0 40.0 15.0 40.0 38-39 33.876467978082175 37.0 33.0 40.0 15.0 40.0 40-41 34.475757457114256 37.0 33.0 40.0 18.5 40.0 42-43 34.10388745019735 37.0 33.0 40.0 15.0 40.0 44-45 33.54066021958845 37.0 33.0 40.0 15.0 40.0 46-47 33.34490880311428 37.0 33.0 40.0 10.5 40.0 48-49 33.22027156778847 37.0 33.0 40.0 10.5 40.0 50-51 32.27409124722438 37.0 33.0 38.5 15.0 38.5 52-53 32.74055954225372 37.0 33.0 38.5 15.0 40.0 54-55 33.41023981714013 37.0 33.0 40.0 15.0 40.0 56-57 33.740600931824666 37.0 33.0 40.0 18.5 40.0 58-59 33.13796654156576 37.0 33.0 40.0 15.0 40.0 60-61 32.93351492541487 37.0 33.0 40.0 10.5 40.0 62-63 32.55839714225521 37.0 33.0 38.5 6.0 40.0 64-65 32.31883505137341 37.0 33.0 37.0 6.0 40.0 66-67 32.14090706549806 37.0 33.0 37.0 10.5 40.0 68-69 32.27721988862103 37.0 33.0 37.0 15.0 38.5 70-71 31.856230528723803 37.0 33.0 37.0 15.0 37.0 72-73 32.19747094806355 37.0 33.0 37.0 18.5 37.0 74-75 32.32329990472941 37.0 33.0 37.0 22.0 37.0 76-77 32.01457807806968 35.0 33.0 37.0 22.0 37.0 78-79 31.588079617127825 33.0 33.0 37.0 18.5 37.0 80-81 31.089493947241348 33.0 33.0 37.0 15.0 37.0 82-83 30.624694099375986 33.0 27.0 37.0 15.0 37.0 84-85 30.389781809842887 33.0 27.0 37.0 15.0 37.0 86-87 30.235150932104325 33.0 27.0 37.0 15.0 37.0 88-89 30.125986684490286 33.0 27.0 37.0 6.0 37.0 90-91 30.036737159444932 33.0 27.0 37.0 6.0 37.0 92-93 29.811461741680603 33.0 27.0 37.0 6.0 37.0 94-95 29.61955806378841 33.0 27.0 37.0 6.0 37.0 96-97 29.49156529018377 33.0 27.0 37.0 6.0 37.0 98-99 29.357646536792906 33.0 27.0 37.0 6.0 37.0 100-101 28.081853283292972 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.10363131499939371 2306 2 0.013778749978545335 2306 3 -0.03173168836078588 2306 4 0.2505310884379668 2306 5 0.17348661326926162 2306 6 -0.21200953038197667 2306 7 0.43052339032050924 2306 8 -0.08792447398310088 2306 9 0.25181337544812266 2306 10-11 0.19358835615137338 2306 12-13 0.2058264055324983 2306 14-15 0.577859558643631 2306 16-17 0.5672097480451157 2306 18-19 0.5725544064345343 2306 20-21 0.44744571924517373 2306 22-23 0.15235808503837944 2306 24-25 0.6368001090250957 2306 26-27 0.0923874213326954 2306 28-29 0.35640881347295306 2306 30-31 0.36727605252448114 2306 32-33 0.44891204898043213 2306 34-35 0.21332592712015241 2306 36-37 0.4993067775984059 2306 38-39 0.2968689553371604 2306 40-41 0.0028423666492756183 2306 42-43 0.4785589674303736 2306 44-45 0.8235918945261815 2306 46-47 0.8283148429876164 2306 48-49 0.423856000865392 2306 50-51 0.48978271651263583 2306 52-53 0.3677547434695896 2306 54-55 0.3722721751188516 2306 56-57 0.23960347319803077 2306 58-59 0.7243658651660212 2306 60-61 0.6804262120710121 2306 62-63 0.6705646980117379 2306 64-65 0.5617143109910288 2306 66-67 0.5662935012096781 2306 68-69 0.34851187778538417 2306 70-71 0.5632934695441669 2306 72-73 0.056416643008724066 2306 74-75 0.011556505625009095 2306 76-77 0.11887846442988348 2306 78-79 0.26004506779450054 2306 80-81 0.6086491383260935 2306 82-83 0.5901639142566069 2306 84-85 0.4711812485010505 2306 86-87 0.5374327225915394 2306 88-89 0.4131522150628051 2306 90-91 0.4228777129620198 2306 92-93 0.48691610878608316 2306 94-95 0.5227163139602737 2306 96-97 0.44567813055663663 2306 98-99 0.46001466375985345 2306 100-101 0.4518542270998438 2307 1 0.04620687776634824 2307 2 -0.02254456199779753 2307 3 -0.047170784430736745 2307 4 0.17520871262273374 2307 5 0.10124522451727813 2307 6 -0.19894864861729644 2307 7 0.3717575062219467 2307 8 -0.20624845874375097 2307 9 0.18694575488804333 2307 10-11 0.08097586687550518 2307 12-13 0.03573479734330931 2307 14-15 0.396444703670376 2307 16-17 0.3572950456217825 2307 18-19 0.41028156892758716 2307 20-21 0.25831170739061093 2307 22-23 -0.039959656909722696 2307 24-25 0.21210905523103207 2307 26-27 -0.1556798883906083 2307 28-29 0.1534557336593565 2307 30-31 0.20821536894432313 2307 32-33 0.28388105657141693 2307 34-35 0.003951929451424974 2307 36-37 0.31180139923546335 2307 38-39 0.056629152061375976 2307 40-41 -0.05610749005809623 2307 42-43 0.20182713456252088 2307 44-45 0.4155715493200347 2307 46-47 0.4540908266739052 2307 48-49 0.19094380964861557 2307 50-51 -0.08711744281728784 2307 52-53 0.09578833574664714 2307 54-55 0.2796036987145456 2307 56-57 0.15475989555012148 2307 58-59 0.3158710505598279 2307 60-61 0.35866672463877336 2307 62-63 0.3687604365229049 2307 64-65 0.4504426964912227 2307 66-67 0.34543001644772886 2307 68-69 0.014145116459523877 2307 70-71 0.17931042996723434 2307 72-73 -0.0950088513443248 2307 74-75 0.02161528298294968 2307 76-77 0.0629916017170018 2307 78-79 0.16149025021552887 2307 80-81 0.43335218749932736 2307 82-83 0.5330176671152884 2307 84-85 0.5944462864038407 2307 86-87 0.5134229281036795 2307 88-89 0.47859144151731314 2307 90-91 0.39400548721941675 2307 92-93 0.6666791218167205 2307 94-95 0.5587677741369177 2307 96-97 0.4338060951754841 2307 98-99 0.34889233957690635 2307 100-101 0.36405626579276884 2201 1 -0.050288134447900035 2201 2 -0.21253206721559792 2201 3 -0.05889101585077228 2201 4 0.1048070054914021 2201 5 -0.01843067009819066 2201 6 -0.15150712231225327 2201 7 0.38787593822572575 2201 8 -0.18548691259419314 2201 9 0.01898789864532091 2201 10-11 -0.050052190394225704 2201 12-13 -0.008301103446207492 2201 14-15 0.35911272962282936 2201 16-17 0.11214355424486655 2201 18-19 0.11955450390699696 2201 20-21 0.12743637188580692 2201 22-23 0.09609622901270853 2201 24-25 0.3339860290026948 2201 26-27 -0.03676434329380385 2201 28-29 0.19256647393387993 2201 30-31 0.22025571077102768 2201 32-33 0.3411106278818892 2201 34-35 0.08588855317829314 2201 36-37 0.34715788466066044 2201 38-39 0.06089511794622382 2201 40-41 -0.017003151991708876 2201 42-43 0.41042271592142754 2201 44-45 0.5452601482445019 2201 46-47 0.4648365256122773 2201 48-49 0.22477424146241987 2201 50-51 0.14209406355709575 2201 52-53 0.2540159996647091 2201 54-55 0.3666767558248054 2201 56-57 0.46547791366860025 2201 58-59 0.6364656969635405 2201 60-61 0.5247578133936344 2201 62-63 0.4027263623415678 2201 64-65 0.5513568619703761 2201 66-67 0.4153215257927343 2201 68-69 0.3017640840371172 2201 70-71 0.4111674451460594 2201 72-73 0.0716325945024252 2201 74-75 0.13881817920222517 2201 76-77 0.3493592286140057 2201 78-79 0.49344113340596607 2201 80-81 0.7018011978692762 2201 82-83 0.5497490814468762 2201 84-85 0.45986919415640415 2201 86-87 0.5024266118178353 2201 88-89 0.5483142817789606 2201 90-91 0.581750706341392 2201 92-93 0.5675898184631016 2201 94-95 0.4349502328781796 2201 96-97 0.3287859026110347 2201 98-99 0.34980354839994376 2201 100-101 0.25124202504692406 1101 1 -0.023201169744645256 1101 2 -0.07094013517282605 1101 3 -0.0728479608081507 1101 4 0.1411227097840353 1101 5 0.09511617170804243 1101 6 -0.06475533584281834 1101 7 0.4298282625710783 1101 8 -0.11598637950775981 1101 9 0.14637592041912484 1101 10-11 0.15292503545441605 1101 12-13 0.13567774379325925 1101 14-15 0.43032454164681866 1101 16-17 0.3278770605144459 1101 18-19 0.4656068156090001 1101 20-21 0.338412618044849 1101 22-23 0.2810157053826927 1101 24-25 0.32227628678862885 1101 26-27 0.08097807143223434 1101 28-29 0.22873349966117473 1101 30-31 0.3635900194054784 1101 32-33 0.11978850998183077 1101 34-35 -0.010508386358559108 1101 36-37 0.22552395035570783 1101 38-39 -0.06447950096564625 1101 40-41 -0.2623088249074925 1101 42-43 0.07978700950416595 1101 44-45 0.2945995500845342 1101 46-47 0.1965820323984886 1101 48-49 0.08053026969727028 1101 50-51 0.2476785439663729 1101 52-53 0.12710159289331813 1101 54-55 0.35566716480889227 1101 56-57 0.43679804540461475 1101 58-59 0.45990331790675043 1101 60-61 0.4701186676212217 1101 62-63 0.39769705450498094 1101 64-65 0.5310477104301441 1101 66-67 0.3824747988662267 1101 68-69 0.25290842096135435 1101 70-71 0.47789801711363467 1101 72-73 0.01067983047710186 1101 74-75 0.1434544535489124 1101 76-77 0.17737331199248274 1101 78-79 0.4181876478015667 1101 80-81 0.7362219881718914 1101 82-83 0.6136708769754868 1101 84-85 0.5350081632169612 1101 86-87 0.5319645029474422 1101 88-89 0.6356803036648984 1101 90-91 0.44324833758540905 1101 92-93 0.5191421702288679 1101 94-95 0.65488453284161 1101 96-97 0.5168898241614137 1101 98-99 0.5018362340773379 1101 100-101 0.4663615228901783 2304 1 0.01036402221494015 2304 2 0.01017276932770983 2304 3 -0.04725010915625205 2304 4 0.13543148072621847 2304 5 0.22297356724170214 2304 6 -0.013721079850782303 2304 7 0.5435666257911862 2304 8 0.12307355759541139 2304 9 0.21322673000132397 2304 10-11 0.19551838644552078 2304 12-13 0.2626258359467073 2304 14-15 0.5181800902570401 2304 16-17 0.29282365822366785 2304 18-19 0.3699493890537937 2304 20-21 0.28100172153468606 2304 22-23 0.17786798996781528 2304 24-25 0.37821513883061897 2304 26-27 0.24989464585838306 2304 28-29 0.45261506495624815 2304 30-31 0.5602127698675332 2304 32-33 0.4676155432775886 2304 34-35 0.20217921689692986 2304 36-37 0.5452715135367541 2304 38-39 0.3631405416615152 2304 40-41 0.10245147443779956 2304 42-43 0.5038741242758746 2304 44-45 0.4814418477587239 2304 46-47 0.46762533704745834 2304 48-49 0.4004248048748238 2304 50-51 0.44021967816331653 2304 52-53 0.2537056585761732 2304 54-55 0.48231391682286073 2304 56-57 0.5853315797440857 2304 58-59 0.8024047030993273 2304 60-61 0.5592300819038982 2304 62-63 0.6528810135734417 2304 64-65 0.6041498486772312 2304 66-67 0.5667199925332724 2304 68-69 0.5109338738485363 2304 70-71 0.5141185313190881 2304 72-73 0.3473863349018984 2304 74-75 0.5871013116447514 2304 76-77 0.5564920674788425 2304 78-79 0.6546730919739758 2304 80-81 0.8144043281182256 2304 82-83 0.6456845687218511 2304 84-85 0.721960240836772 2304 86-87 0.6034230061280006 2304 88-89 0.5571464354422524 2304 90-91 0.5274176467895515 2304 92-93 0.6379219582088211 2304 94-95 0.5249925630702919 2304 96-97 0.38383758665804635 2304 98-99 0.4797121411090437 2304 100-101 0.3516867018663561 2202 1 0.17103292644143409 2202 2 0.19151988765372963 2202 3 0.24952384582137555 2202 4 0.3583101311716703 2202 5 0.4276406849006591 2202 6 0.3076286922240925 2202 7 0.5366741294473414 2202 8 0.35793323156133283 2202 9 0.33247612511334523 2202 10-11 0.39772716107336237 2202 12-13 0.44071105463809346 2202 14-15 0.5664583800536676 2202 16-17 0.5576654375115169 2202 18-19 0.5313643882453363 2202 20-21 0.5688254372036283 2202 22-23 0.35733917612829913 2202 24-25 0.42820495162531813 2202 26-27 0.37747162813989377 2202 28-29 0.6724665565298764 2202 30-31 0.6307838189176493 2202 32-33 0.6579678248127934 2202 34-35 0.4079725464163957 2202 36-37 0.521205781772764 2202 38-39 0.5080705762255064 2202 40-41 0.46073983542885344 2202 42-43 0.7242262752988324 2202 44-45 0.7136733315161123 2202 46-47 0.5843567522859203 2202 48-49 0.6541911705657668 2202 50-51 0.5314422950642239 2202 52-53 0.5438523108284841 2202 54-55 0.6370920061381824 2202 56-57 0.7452879234002054 2202 58-59 0.7951085251502406 2202 60-61 0.6602695756324266 2202 62-63 0.7117568971594253 2202 64-65 0.7472425609463258 2202 66-67 0.8073236551417295 2202 68-69 0.7004439889095764 2202 70-71 0.7033070787251283 2202 72-73 0.5590191807720188 2202 74-75 0.6951264729542572 2202 76-77 0.7158052758249553 2202 78-79 0.6364597465175024 2202 80-81 0.6917072346982884 2202 82-83 0.6503540065983913 2202 84-85 0.7080654003481079 2202 86-87 0.6831170419362032 2202 88-89 0.5806275811366888 2202 90-91 0.536134232937421 2202 92-93 0.9095592559964203 2202 94-95 0.8216334680711626 2202 96-97 0.7019564557549991 2202 98-99 0.8696702031763159 2202 100-101 0.9584638398924739 1102 1 -0.04398184409992467 1102 2 -0.25580458335478795 1102 3 -0.10154650317560154 1102 4 -0.194303567646692 1102 5 -0.11054116450851836 1102 6 0.022646554274110997 1102 7 -0.07775703295991576 1102 8 -0.05508717239579397 1102 9 -0.12877524889049852 1102 10-11 -0.18388849570047228 1102 12-13 -0.1901061341500352 1102 14-15 -0.3350339598748846 1102 16-17 -0.2922016276162651 1102 18-19 -0.16870551420038993 1102 20-21 -0.2546210796966193 1102 22-23 -0.08605461337431564 1102 24-25 -0.17294526134527644 1102 26-27 0.02595884990412145 1102 28-29 -0.06808854102439454 1102 30-31 -0.11429140779298308 1102 32-33 -0.11621361239522798 1102 34-35 -0.06752724748302086 1102 36-37 -0.08359996843334017 1102 38-39 -0.16390816106495976 1102 40-41 -0.020097448165543597 1102 42-43 0.08342007854148648 1102 44-45 -0.1400258303415427 1102 46-47 -0.17967457500179762 1102 48-49 -0.20302561289122423 1102 50-51 0.06455784878777138 1102 52-53 -0.0024734311335947723 1102 54-55 0.08538394760562085 1102 56-57 0.2711808541504084 1102 58-59 0.182960441885605 1102 60-61 -0.002907121262325063 1102 62-63 0.02032329589533788 1102 64-65 0.07561642739315033 1102 66-67 0.1237670461832252 1102 68-69 0.12144838572189087 1102 70-71 0.214325215575915 1102 72-73 0.18497153718862336 1102 74-75 0.31553798710649517 1102 76-77 0.4829756866521038 1102 78-79 0.24969123181566033 1102 80-81 0.1265327362096933 1102 82-83 0.16311695658465197 1102 84-85 0.14996101040581422 1102 86-87 0.08441684957566764 1102 88-89 0.043628405538999715 1102 90-91 0.103095966930276 1102 92-93 0.2195913733363497 1102 94-95 -0.03396027334131446 1102 96-97 0.052903659657165036 1102 98-99 0.10056691243709892 1102 100-101 0.13086216360778025 2305 1 0.2169239325930299 2305 2 0.16740248261091395 2305 3 0.21573913958319224 2305 4 0.231785273259554 2305 5 0.3206306029216961 2305 6 0.3305762075473595 2305 7 0.31111078529017533 2305 8 0.37626140064212876 2305 9 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78-79 -0.21445209593779424 1213 80-81 -0.5323246764437535 1213 82-83 -0.4560564166662715 1213 84-85 -0.5011658567072423 1213 86-87 -0.31873191855893523 1213 88-89 -0.283031393585393 1213 90-91 -0.3405847138148559 1213 92-93 -0.2916017011764822 1213 94-95 -0.2744788264360025 1213 96-97 -0.3321833113274657 1213 98-99 -0.4098332828586315 1213 100-101 -0.18521925969035635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 370.0 3 222.0 4 200.0 5 203.0 6 519.0 7 3432.0 8 18436.0 9 55263.0 10 76960.0 11 55940.0 12 31775.0 13 19639.0 14 13755.0 15 10748.0 16 9661.0 17 8541.0 18 8665.0 19 8875.0 20 9231.0 21 9830.0 22 10972.0 23 11361.0 24 12850.0 25 14869.0 26 17647.0 27 21592.0 28 27371.0 29 33577.0 30 40768.0 31 54346.0 32 75412.0 33 108896.0 34 167820.0 35 291025.0 36 549417.0 37 759359.0 38 142232.0 39 56.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.4950548621086 20.397143074938604 24.41438566545249 11.693416397500304 2 34.400819267277356 24.01430504629488 31.177027936834822 10.407847749592944 3 39.70833554336531 16.71482668742478 29.718966108443716 13.857871660766193 4 31.522936670662798 14.122177032331312 35.335880399911865 19.01900589709403 5 35.88159340115496 12.868890758920218 34.83360394006388 16.415911899860944 6 31.755023163151623 13.01224764709572 40.74974835647657 14.482980833276086 7 39.83856595318473 15.448115364643316 28.63822967580666 16.075089006365303 8 30.820998122701205 32.184083537369965 21.555488943199112 15.439429396729714 9 36.50501627925292 27.901777299521456 19.43772285295074 16.155483568274885 10-11 31.627795799005508 28.285320403828095 24.603684341734226 15.483199455432167 12-13 33.62205185883938 21.658499601881257 24.690156108089706 20.029292431189663 14-15 29.2850635729445 22.492352825129284 25.995056609344662 22.227526992581552 16-17 30.342419877115457 24.82306629841987 27.278480386640474 17.5560334378242 18-19 29.88602807254313 24.963125302312804 27.25047623597418 17.900370389169886 20-21 27.76522139145413 28.108284135750804 26.545363950138984 17.581130522656082 22-23 32.15397232207749 24.03935702430145 25.62121383168397 18.18545682193708 24-25 28.69532698665348 27.36206003622485 25.964575112287868 17.978037864833798 26-27 24.096055845848603 29.60827810252328 27.28157943990523 19.01408661172289 28-29 25.40286192634768 28.90383637860549 25.34010660380809 20.35319509123874 30-31 27.143712752660015 26.440202466039803 26.525251200981888 19.890833580318297 32-33 23.921213753854442 26.863499021280745 28.962972317507813 20.252314907357004 34-35 29.58641364016188 24.2216031251512 26.047547178129772 20.14443605655715 36-37 24.297804447898606 28.05574445507264 28.812847492078674 18.83360360495008 38-39 23.186099717855793 30.717380882721645 24.75930308027042 21.33721631915214 40-41 29.910703026614232 24.948358928286865 23.789360846343783 21.351577198755123 42-43 30.987791342952274 25.447015474762424 24.56841818107079 18.996775001214512 44-45 25.33305535519833 28.897900760684927 25.40449274688167 20.36455113723508 46-47 25.989340184514774 29.32650706528086 25.548317178558914 19.13583557164545 48-49 22.645646118610134 27.48652633164047 28.71634945839095 21.15147809135845 50-51 19.930803295344525 26.281538440267866 29.1712986507801 24.616359613607514 52-53 26.528103907497457 26.820140110869882 25.29926144656935 21.352494535063308 54-55 28.27642127296252 26.437402088175414 25.834339029925086 19.451837608936973 56-57 29.488078285931724 25.81251299432369 23.968304404442943 20.73110431530165 58-59 26.65879028047718 24.591117491087697 24.942192617802565 23.80789961063256 60-61 21.60029522213748 24.69474912467981 26.586034618381316 27.118921034801403 62-63 25.226773089475117 24.69828166527644 26.85469174788147 23.220253497366972 64-65 23.263600670404625 28.02836614550021 27.96754419955275 20.740488984542413 66-67 21.033183385484506 27.783189828896592 26.623151534484577 24.56047525113432 68-69 19.916219333459825 23.96914782837345 34.32280749458046 21.791825343586265 70-71 19.525036843603104 30.875312881180967 29.870675778533528 19.728974496682394 72-73 21.285367580819376 34.857039453928586 25.179909854593095 18.67768311065894 74-75 22.383734012078715 33.85601109615001 25.011413775068057 18.748841116703215 76-77 21.472451411482567 32.71008088717813 25.023576687073028 20.79389101426628 78-79 21.68263256789881 33.80415592738985 24.96454684764799 19.548664657063348 80-81 20.979717060766646 34.3130117983479 24.69648798576489 20.01078315512056 82-83 20.586759587609148 33.2686637645684 25.61826498757418 20.52631166024828 84-85 21.26293228568728 31.8384066236017 25.849328901559403 21.049332189151617 86-87 19.56528439655409 32.228570809454524 26.964606714392218 21.241538079599167 88-89 19.474946995337607 32.26787843666277 27.000441448646516 21.256733119353104 90-91 20.98209332688317 30.91393702125752 25.931260938349272 22.17270871351004 92-93 21.185851797004045 31.198989334037986 24.92352310086958 22.69163576808839 94-95 21.51319299573153 32.487694797743124 24.6634965803891 21.335615626136235 96-97 20.895095622291947 33.37423427461527 23.72252726729419 22.008142835798594 98-99 21.290061677892716 31.008439149584767 25.255677466835525 22.445821705686996 100-101 22.652204217120566 31.63197234292841 24.820506823384093 20.89531661656693 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 153.0 1 170.5 2 188.5 3 218.5 4 238.5 5 216.5 6 229.5 7 306.5 8 406.5 9 473.0 10 531.0 11 665.0 12 758.0 13 712.0 14 654.0 15 637.0 16 617.5 17 563.0 18 543.0 19 541.0 20 546.5 21 580.0 22 655.5 23 719.5 24 813.0 25 1021.0 26 1259.0 27 1552.5 28 1905.5 29 2368.0 30 3092.5 31 4355.5 32 6709.0 33 10806.5 34 17424.5 35 28913.0 36 45625.0 37 66493.0 38 91567.5 39 110245.0 40 118743.0 41 121224.0 42 150593.0 43 187985.0 44 180987.5 45 161622.0 46 162133.5 47 156372.0 48 134044.5 49 117191.0 50 103931.5 51 97731.0 52 97914.0 53 100069.0 54 86909.5 55 58634.0 56 42540.0 57 36325.0 58 31809.0 59 26308.5 60 20983.0 61 17146.0 62 13782.0 63 10927.5 64 8817.5 65 7004.0 66 5588.5 67 4523.0 68 3529.0 69 2706.5 70 2120.0 71 1687.5 72 1267.5 73 869.0 74 602.0 75 452.5 76 340.0 77 246.5 78 176.0 79 134.0 80 110.5 81 79.5 82 55.5 83 40.0 84 32.0 85 28.5 86 22.0 87 15.5 88 16.5 89 16.0 90 10.5 91 8.0 92 9.5 93 9.5 94 5.5 95 3.5 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21145969084600658 2 0.1975140155900717 3 0.1733514552535857 4 0.15828714294503576 5 0.16846674012383311 6 0.1283822457384589 7 0.11536876802637 8 0.11134167463695567 9 0.0991112428616973 10-11 0.08497912809699329 12-13 0.11134167463695567 14-15 0.10830271064401799 16-17 0.09894344730380505 18-19 0.09288416326880662 20-21 0.09648244578805185 22-23 0.084363877718055 24-25 0.39019924790302163 26-27 0.18785644903582807 28-29 0.08878249407588461 30-31 0.16952944532381747 32-33 0.20053433563213247 34-35 0.19973264574442498 36-37 0.20564277817240809 38-39 0.20672412732326934 40-41 0.2024173746707012 42-43 0.21869354378625086 44-45 0.20006823686020953 46-47 0.2199054005932505 48-49 0.20868174216534574 50-51 0.21239188838985246 52-53 0.203386860116301 54-55 0.18645815272005922 56-57 0.19335641454451896 58-59 0.1885835631200279 60-61 0.16887690704312533 62-63 0.15711257403978993 64-65 0.16210915287480399 66-67 0.1745819560114623 68-69 0.1673108151694642 70-71 0.16037526544325062 72-73 0.1634328733870652 74-75 0.1547261483275444 76-77 0.1641413435203881 78-79 0.16250067584321928 80-81 0.16820572481155627 82-83 0.1920699819340116 84-85 0.19031745055158128 86-87 0.1943258999901187 88-89 0.2020817835549167 90-91 0.18271071859379864 92-93 0.16378710845372665 94-95 0.1644582906852957 96-97 0.172829424629032 98-99 0.1873717063130282 100-101 0.1991360393163636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2681835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.85047586814573 #Duplication Level Percentage of deduplicated Percentage of total 1 88.12141026628817 37.7604436408255 2 5.173827466984924 4.43401938039974 3 1.7722632668890566 2.278269730494919 4 1.0154363060562583 1.7404771571321094 5 0.677378413842627 1.4512993687983156 6 0.4760044752628628 1.2238210968228405 7 0.31092652772388146 0.932634477109897 8 0.21130696773157853 0.7243683297242444 9 0.193623905347481 0.7467188835229531 >10 1.629838490564404 13.718167704758018 >50 0.21302680255235848 6.380592116865218 >100 0.18267278488339483 15.519734600010715 >500 0.015005952924947785 4.426642777670374 >1k 0.0065733240888754445 4.604060471741162 >5k 2.6439332310147806E-4 0.6159028455291675 >10k+ 4.40655536293241E-4 3.442847418594698 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31479 1.173785859309018 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24214 0.902889253067396 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12126 0.4521530966670209 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11847 0.4417497720777005 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 11500 0.4288108701691193 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6038 0.22514435078966455 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5213 0.19438183184274946 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5058 0.1886022070709048 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3622 0.13505678015239567 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3458 0.1289415642647665 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2706 0.10090106214588146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00432539660344503 0.0 0.0 2 0.00473556352273723 0.0 0.0 3 0.005183018343783268 0.0 0.0 4 0.005444033656060123 0.0 0.0 5 0.006413519101659871 0.0 0.0 6 0.0068609739227059085 0.0 0.0 7 0.007755883564797983 0.0 0.0 8 0.00883723271565924 0.0 0.0 9 0.010179597178797354 0.0 0.0 10-11 0.015959221950642005 0.0 3.728790175383646E-5 12-13 0.021235460048809864 0.0 3.728790175383646E-5 14-15 0.027593047297838978 0.0 3.728790175383646E-5 16-17 0.03361504343108357 0.0 3.728790175383646E-5 18-19 0.03982347907309734 0.0 3.728790175383646E-5 20-21 0.045267512729157466 0.0 3.728790175383646E-5 22-23 0.05035731131855614 0.0 3.728790175383646E-5 24-25 0.05598778448338544 0.0 3.728790175383646E-5 26-27 0.06089114356401494 0.0 3.728790175383646E-5 28-29 0.06736059451830556 0.0 3.728790175383646E-5 30-31 0.1183331562158 0.0 3.728790175383646E-5 32-33 0.12776699535952063 0.0 3.728790175383646E-5 34-35 0.13561609867870322 0.0 3.728790175383646E-5 36-37 0.14381943706454722 0.0 3.728790175383646E-5 38-39 0.1531973443556371 0.0 3.728790175383646E-5 40-41 0.16330236573092677 0.0 3.728790175383646E-5 42-43 0.1740785693377855 0.0 3.728790175383646E-5 44-45 0.18753950187092044 0.0 3.728790175383646E-5 46-47 0.20252923837596273 0.0 3.728790175383646E-5 48-49 0.21707152005995894 0.0 3.728790175383646E-5 50-51 0.2323782037299088 0.0 3.728790175383646E-5 52-53 0.2495306385366736 0.0 3.728790175383646E-5 54-55 0.26808136965920726 0.0 3.728790175383646E-5 56-57 0.28885073093609415 0.0 3.728790175383646E-5 58-59 0.31217431348311886 0.0 3.728790175383646E-5 60-61 0.34002837609323466 0.0 3.728790175383646E-5 62-63 0.3713875014682111 0.0 3.728790175383646E-5 64-65 0.4033805211730028 0.0 3.728790175383646E-5 66-67 0.4386548762321321 0.0 3.728790175383646E-5 68-69 0.47765802146664504 0.0 3.728790175383646E-5 70-71 0.52027809317128 0.0 3.728790175383646E-5 72-73 0.5689201610091598 0.0 3.728790175383646E-5 74-75 0.6201164501171772 0.0 3.728790175383646E-5 76-77 0.676514401519855 0.0 3.728790175383646E-5 78-79 0.7426444952802838 0.0 3.728790175383646E-5 80-81 0.8188982543668794 0.0 3.728790175383646E-5 82-83 0.9018079039165348 0.0 3.728790175383646E-5 84-85 0.995009014350249 0.0 3.728790175383646E-5 86-87 1.098277858257499 0.0 3.728790175383646E-5 88-89 1.212509345280377 0.0 3.728790175383646E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3270 0.0 88.80906 9 ATCGGGA 5355 0.0 87.69486 2 CGGGAGA 4615 0.0 85.07744 4 AGAGCGG 3605 0.0 83.84861 8 ATCCGGA 815 0.0 82.14835 2 TCGGGAG 4880 0.0 81.723724 3 AAGCGGC 930 0.0 80.6172 9 GTTCGGA 495 0.0 79.617805 2 CGGAGAG 1680 0.0 78.84377 5 TCGGAGA 990 0.0 78.16872 4 ATTCGGA 825 0.0 77.69932 2 CCGGAGA 835 0.0 73.91583 4 GAGAGCG 4935 0.0 72.11804 7 TTGGGAG 2230 0.0 71.96155 3 TCCGGAG 780 0.0 71.82507 3 TCGGGAA 1400 0.0 71.55552 3 AAGCGTC 480 0.0 70.18926 9 AAGGGGC 2600 0.0 69.71779 9 CGGAAAG 1860 0.0 68.15077 5 GTCCGGA 265 0.0 68.08871 2 >>END_MODULE