FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681835
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA314791.173785859309018No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC242140.902889253067396No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC121260.4521530966670209No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT118470.4417497720777005No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT115000.4288108701691193No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT60380.22514435078966455No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC52130.19438183184274946No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT50580.1886022070709048No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC36220.13505678015239567No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG34580.1289415642647665No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27060.10090106214588146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC32700.088.809069
ATCGGGA53550.087.694862
CGGGAGA46150.085.077444
AGAGCGG36050.083.848618
ATCCGGA8150.082.148352
TCGGGAG48800.081.7237243
AAGCGGC9300.080.61729
GTTCGGA4950.079.6178052
CGGAGAG16800.078.843775
TCGGAGA9900.078.168724
ATTCGGA8250.077.699322
CCGGAGA8350.073.915834
GAGAGCG49350.072.118047
TTGGGAG22300.071.961553
TCCGGAG7800.071.825073
TCGGGAA14000.071.555523
AAGCGTC4800.070.189269
AAGGGGC26000.069.717799
CGGAAAG18600.068.150775
GTCCGGA2650.068.088712
Produced by FastQC (version 0.11.2)