##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_30245-1.3420000003577e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2252931 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968878318954285 33.0 33.0 33.0 33.0 33.0 2 31.882627563826855 33.0 33.0 33.0 33.0 33.0 3 31.83777798787446 33.0 33.0 33.0 33.0 33.0 4 35.551438104407104 37.0 37.0 37.0 33.0 37.0 5 35.51011504568937 37.0 37.0 37.0 33.0 37.0 6 35.64228686985975 37.0 37.0 37.0 33.0 37.0 7 35.516417946222056 37.0 37.0 37.0 33.0 37.0 8 35.531687388561835 37.0 37.0 37.0 33.0 37.0 9 35.419325314445935 37.0 37.0 37.0 33.0 37.0 10-11 35.37643518598661 37.0 37.0 37.0 33.0 37.0 12-13 35.38025643040111 37.0 37.0 37.0 33.0 37.0 14-15 37.18780979089018 40.0 37.0 40.0 33.0 40.0 16-17 37.157281780933374 40.0 37.0 40.0 33.0 40.0 18-19 37.15560951489415 40.0 37.0 40.0 33.0 40.0 20-21 37.12477989783087 40.0 37.0 40.0 33.0 40.0 22-23 37.26671300630157 40.0 37.0 40.0 33.0 40.0 24-25 37.021321780382976 40.0 37.0 40.0 33.0 40.0 26-27 36.9237741857163 40.0 37.0 40.0 33.0 40.0 28-29 36.85178618430835 40.0 37.0 40.0 33.0 40.0 30-31 36.83938833457394 40.0 37.0 40.0 33.0 40.0 32-33 36.798083030505595 40.0 37.0 40.0 33.0 40.0 34-35 36.75421639632994 40.0 37.0 40.0 33.0 40.0 36-37 36.660009117012464 40.0 37.0 40.0 33.0 40.0 38-39 36.65478569916256 40.0 37.0 40.0 33.0 40.0 40-41 36.502568653900184 40.0 37.0 40.0 33.0 40.0 42-43 36.43005977546583 40.0 37.0 40.0 33.0 40.0 44-45 36.335759062305954 40.0 37.0 40.0 33.0 40.0 46-47 36.24791305193102 40.0 37.0 40.0 33.0 40.0 48-49 36.14758352563838 40.0 37.0 40.0 33.0 40.0 50-51 35.044468294856784 37.0 35.0 38.5 27.0 38.5 52-53 35.44416473473888 37.0 37.0 38.5 30.0 40.0 54-55 36.22440256714476 38.5 37.0 40.0 33.0 40.0 56-57 36.30322633050014 40.0 37.0 40.0 33.0 40.0 58-59 36.10529261659589 37.0 37.0 40.0 33.0 40.0 60-61 35.926613154153415 37.0 37.0 40.0 27.0 40.0 62-63 35.73131689341574 37.0 37.0 40.0 27.0 40.0 64-65 35.515061934875064 37.0 37.0 40.0 27.0 40.0 66-67 35.28069190756396 37.0 37.0 40.0 27.0 40.0 68-69 35.10136750748248 37.0 37.0 40.0 27.0 40.0 70-71 34.79488674974955 37.0 33.0 37.0 27.0 40.0 72-73 34.618880693638644 37.0 33.0 37.0 27.0 40.0 74-75 34.39838193002804 37.0 33.0 37.0 27.0 40.0 76-77 34.138194423175854 37.0 33.0 37.0 27.0 38.5 78-79 33.86643887451502 37.0 33.0 37.0 27.0 37.0 80-81 33.613006789821796 37.0 33.0 37.0 27.0 37.0 82-83 33.37392490049629 37.0 33.0 37.0 27.0 37.0 84-85 33.182316724302694 37.0 33.0 37.0 27.0 37.0 86-87 33.00468922483644 37.0 33.0 37.0 27.0 37.0 88-89 32.81857833195957 37.0 33.0 37.0 27.0 37.0 90-91 32.674788309096016 37.0 33.0 37.0 27.0 37.0 92-93 32.5033019209199 37.0 33.0 37.0 22.0 37.0 94-95 32.37406116743034 33.0 33.0 37.0 22.0 37.0 96-97 32.24778055785996 33.0 33.0 37.0 22.0 37.0 98-99 32.10256772178109 33.0 33.0 37.0 22.0 37.0 100-101 30.850420407904192 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.24596395671884963 2306 2 0.22583397478341638 2306 3 0.17089814288651084 2306 4 0.24629335884743853 2306 5 0.3722613477635548 2306 6 0.2585154361601312 2306 7 0.39820977874619956 2306 8 0.20606085130447838 2306 9 0.29943410201707366 2306 10-11 0.22155333609050842 2306 12-13 0.20523880139528217 2306 14-15 0.45674621946508864 2306 16-17 0.3849275942982828 2306 18-19 0.42925239507311375 2306 20-21 0.39410594370721697 2306 22-23 0.2446996660756895 2306 24-25 0.3341830249923774 2306 26-27 0.3323688377741618 2306 28-29 0.3006774972542985 2306 30-31 0.2688813103197347 2306 32-33 0.15379807461029316 2306 34-35 0.2671776056022708 2306 36-37 0.20456838561025137 2306 38-39 0.05128078837930872 2306 40-41 0.10417496716820551 2306 42-43 0.2413839400811355 2306 44-45 0.3535061701450246 2306 46-47 0.3854794174148992 2306 48-49 0.3869635511350751 2306 50-51 0.4291327969378784 2306 52-53 0.3253001911424178 2306 54-55 0.28128359939213254 2306 56-57 0.1571925081489951 2306 58-59 0.19611169236736004 2306 60-61 0.21950770955530174 2306 62-63 0.2793072642541077 2306 64-65 0.3331323202460581 2306 66-67 0.2907161771805633 2306 68-69 0.20323249795090703 2306 70-71 0.20576944518490592 2306 72-73 2.2284538666639264E-4 2306 74-75 0.17191015795793874 2306 76-77 0.1334595228143911 2306 78-79 0.066468493691346 2306 80-81 0.14214743478162717 2306 82-83 0.2299509186744828 2306 84-85 0.18929850201636356 2306 86-87 0.2352513209256486 2306 88-89 0.23435880026529787 2306 90-91 0.11567220808917966 2306 92-93 0.24639159929191123 2306 94-95 0.2652132527690796 2306 96-97 0.036933635692470546 2306 98-99 0.037078566350764675 2306 100-101 0.06454371260012337 2307 1 0.19364836028175247 2307 2 0.21499558317652756 2307 3 0.16518211502795666 2307 4 0.1779151266336143 2307 5 0.2575328858974757 2307 6 0.13474834246385115 2307 7 0.3549542626490876 2307 8 0.25950211149635294 2307 9 0.242035974778382 2307 10-11 0.3119792958347247 2307 12-13 0.2127888683452781 2307 14-15 0.4290560018240015 2307 16-17 0.5202119632371662 2307 18-19 0.5525702190911446 2307 20-21 0.4174951606221242 2307 22-23 0.39133202023348446 2307 24-25 0.32185989859630837 2307 26-27 0.2741135361621545 2307 28-29 0.4101280936760716 2307 30-31 0.4073360371116834 2307 32-33 0.37118140237723196 2307 34-35 0.33076535145581687 2307 36-37 0.19451267310606823 2307 38-39 0.1766994173292744 2307 40-41 0.32836302616998836 2307 42-43 0.25454087729522 2307 44-45 0.2077692912708926 2307 46-47 0.16908674269380697 2307 48-49 0.1572618845858642 2307 50-51 0.17263562201302562 2307 52-53 0.291122565308612 2307 54-55 0.27717352870397605 2307 56-57 0.15919181566853524 2307 58-59 0.21042723211910896 2307 60-61 0.23098859893308088 2307 62-63 0.295134732441376 2307 64-65 0.28286314144821034 2307 66-67 0.3418869594077165 2307 68-69 0.36250262048318405 2307 70-71 0.2846335091259746 2307 72-73 0.23228193296706223 2307 74-75 0.2347952736113612 2307 76-77 0.313722565806394 2307 78-79 0.24829880993429754 2307 80-81 0.12054304028837493 2307 82-83 0.25284969243253386 2307 84-85 0.3567793822019212 2307 86-87 0.3316238800086282 2307 88-89 0.23240430481084928 2307 90-91 0.31249388364676634 2307 92-93 0.2789674810554672 2307 94-95 0.2696818199779827 2307 96-97 0.19613309304516946 2307 98-99 0.1797582665618762 2307 100-101 0.15296557721100257 2201 1 0.010001538796988285 2201 2 0.005692202768713628 2201 3 -0.0308507499429993 2201 4 0.024767811885645585 2201 5 0.07514714135867706 2201 6 0.07345313053591696 2201 7 0.20777043302299347 2201 8 0.05544577331331624 2201 9 0.10064657036750191 2201 10-11 0.037022600254921656 2201 12-13 0.05036043961622738 2201 14-15 0.25011949172215964 2201 16-17 0.08671092156011895 2201 18-19 0.1918049687364558 2201 20-21 0.19266300927639435 2201 22-23 0.16050992520325735 2201 24-25 0.19929507425883486 2201 26-27 0.2307481238260749 2201 28-29 0.13366824248315368 2201 30-31 0.2294582131325953 2201 32-33 0.0742598045961742 2201 34-35 0.15886475730646055 2201 36-37 0.13202218702104318 2201 38-39 0.10407272462555284 2201 40-41 0.08519594996829483 2201 42-43 0.16280663534506346 2201 44-45 0.2079094872910474 2201 46-47 0.1886102411617827 2201 48-49 0.3681668670768232 2201 50-51 0.23441963011415368 2201 52-53 0.23009726508124118 2201 54-55 0.24911130228809242 2201 56-57 0.19958562216572773 2201 58-59 0.3064740479685426 2201 60-61 0.3139169501763419 2201 62-63 0.19115361217209426 2201 64-65 0.2812782048233444 2201 66-67 0.0933079044869558 2201 68-69 0.12483978435206211 2201 70-71 0.27149047585450603 2201 72-73 0.11725023938883083 2201 74-75 0.08413472013260304 2201 76-77 0.27825743973821915 2201 78-79 0.1281676579319182 2201 80-81 0.18734008696299753 2201 82-83 0.26973661231566837 2201 84-85 0.29609924595063575 2201 86-87 0.26220520211894893 2201 88-89 0.26421213592687565 2201 90-91 0.2140393385983117 2201 92-93 0.22025661782252826 2201 94-95 0.249848721965499 2201 96-97 0.16171479828922486 2201 98-99 0.11076034854150407 2201 100-101 0.1380802304096882 1101 1 0.03556199611152877 1101 2 -0.040125296578356284 1101 3 -0.15211462091988537 1101 4 -0.028425608734544028 1101 5 0.019641638183777843 1101 6 -0.07446886333497105 1101 7 0.09640984734939906 1101 8 -0.048271982549394465 1101 9 -0.052394263601890145 1101 10-11 -0.07037096870028137 1101 12-13 0.010606926782685377 1101 14-15 0.14908229391761552 1101 16-17 0.09641765285171999 1101 18-19 0.15208266834253692 1101 20-21 0.03187727238275073 1101 22-23 0.07835119189516604 1101 24-25 0.006636206315455695 1101 26-27 0.09097207054964684 1101 28-29 0.16849221444662277 1101 30-31 0.12485520499576097 1101 32-33 -0.0598399712816402 1101 34-35 -0.006034272982091693 1101 36-37 0.1030796509782661 1101 38-39 0.043507263197717805 1101 40-41 0.2523718011905771 1101 42-43 0.017546809500352367 1101 44-45 0.04031825754792351 1101 46-47 0.07944481793121838 1101 48-49 0.11175262637593875 1101 50-51 0.254260450046381 1101 52-53 0.1840908039375222 1101 54-55 0.16199451512454033 1101 56-57 0.09764613178219861 1101 58-59 0.2118343879918072 1101 60-61 0.17321295950565485 1101 62-63 0.02131559292363505 1101 64-65 0.0833459530263525 1101 66-67 0.1604227480434588 1101 68-69 0.0561621255077398 1101 70-71 0.24907032739802304 1101 72-73 0.09909269788978037 1101 74-75 0.12157631912732114 1101 76-77 0.18913681395073212 1101 78-79 0.19928376753246368 1101 80-81 0.20600934375600133 1101 82-83 0.10789100220189596 1101 84-85 0.17326513618677808 1101 86-87 0.016088535755969247 1101 88-89 0.09282783554780139 1101 90-91 0.1589099031419252 1101 92-93 0.26423106478871006 1101 94-95 0.21839231916373336 1101 96-97 0.0781162435770213 1101 98-99 0.2006137074386629 1101 100-101 0.08289883045922153 2304 1 0.2609630799176408 2304 2 0.17723384271523557 2304 3 0.1659839461049195 2304 4 0.1464801586052218 2304 5 0.20064634843485862 2304 6 0.20741162563687254 2304 7 0.2602834684078914 2304 8 0.2551624040646203 2304 9 0.18622365328665325 2304 10-11 0.2282476790786987 2304 12-13 0.20480605204809166 2304 14-15 0.33148951536084326 2304 16-17 0.3014377953323617 2304 18-19 0.3494960225093138 2304 20-21 0.4071439091996112 2304 22-23 0.3315053833675776 2304 24-25 0.47827074758343 2304 26-27 0.430355825496207 2304 28-29 0.3993316758970664 2304 30-31 0.4187841771856924 2304 32-33 0.3064817028136986 2304 34-35 0.3087631567403335 2304 36-37 0.3849899283419731 2304 38-39 0.22917416387215894 2304 40-41 0.4411233359069513 2304 42-43 0.32575670526554745 2304 44-45 0.378900182688767 2304 46-47 0.2544029452793666 2304 48-49 0.2987830369459701 2304 50-51 0.31173408117765433 2304 52-53 0.18042437496379193 2304 54-55 0.42993188702677543 2304 56-57 0.38638286443168113 2304 58-59 0.47130003337726833 2304 60-61 0.5055159829967195 2304 62-63 0.5402378243382842 2304 64-65 0.4831886273237629 2304 66-67 0.44402144374183194 2304 68-69 0.3359990386687386 2304 70-71 0.47547024362200574 2304 72-73 0.5109896902449194 2304 74-75 0.5649140378032911 2304 76-77 0.43681270519178383 2304 78-79 0.39708882369740195 2304 80-81 0.5597558635812518 2304 82-83 0.49174406619902555 2304 84-85 0.571108610164309 2304 86-87 0.4514210079898149 2304 88-89 0.38846380543020587 2304 90-91 0.48527628439281045 2304 92-93 0.6317212422968197 2304 94-95 0.37541860235246105 2304 96-97 0.5675101941381158 2304 98-99 0.6766896365140411 2304 100-101 0.59587873834246 2202 1 0.10191626335472037 2202 2 0.11548844759695598 2202 3 0.06491202223811143 2202 4 0.06374971276049735 2202 5 0.21304416816433047 2202 6 0.17105679329853274 2202 7 0.2224523448655944 2202 8 0.19945411347232067 2202 9 0.11854734254394828 2202 10-11 0.1708089075993442 2202 12-13 0.10209045672125683 2202 14-15 0.19503876789715946 2202 16-17 0.2339311213598947 2202 18-19 0.18709204382354727 2202 20-21 0.2731883354503566 2202 22-23 0.18693622890732797 2202 24-25 0.22435901392218938 2202 26-27 0.1408020524983442 2202 28-29 0.2283090077522516 2202 30-31 0.24389145452349226 2202 32-33 0.07512847392776933 2202 34-35 0.1128751626699227 2202 36-37 0.12630845469487184 2202 38-39 0.07816943103281204 2202 40-41 0.16482299971288938 2202 42-43 0.21037649059406505 2202 44-45 0.17178576520922206 2202 46-47 0.2431510365338596 2202 48-49 0.2493002034437879 2202 50-51 0.2466970955832366 2202 52-53 0.13021414748592974 2202 54-55 0.17275882672634424 2202 56-57 0.27373900501729764 2202 58-59 0.26469041138852134 2202 60-61 0.3293759389495605 2202 62-63 0.34328727757163335 2202 64-65 0.4509767549798127 2202 66-67 0.4346468220397952 2202 68-69 0.36417029057217576 2202 70-71 0.3851293719385538 2202 72-73 0.3346525330492369 2202 74-75 0.4335681890460208 2202 76-77 0.3579715606028486 2202 78-79 0.18072083986359644 2202 80-81 0.28785851745428204 2202 82-83 0.3569875626597536 2202 84-85 0.43063171049795557 2202 86-87 0.4262133555928145 2202 88-89 0.33447298758452604 2202 90-91 0.2485209606122254 2202 92-93 0.2881297956004616 2202 94-95 0.37860984431661393 2202 96-97 0.4246320979352234 2202 98-99 0.1698206746804587 2202 100-101 0.10115128277720586 1102 1 0.11966196352436143 1102 2 0.06838998179128453 1102 3 0.11011183484929532 1102 4 0.09733162519956551 1102 5 0.13341682012881506 1102 6 0.17809006513201098 1102 7 0.15193337277992214 1102 8 0.2407260872874204 1102 9 0.11212081423131082 1102 10-11 0.18340580082486468 1102 12-13 0.1251299425164376 1102 14-15 0.11606898331174875 1102 16-17 0.12235996520392689 1102 18-19 0.20213113131431015 1102 20-21 0.11381729527732887 1102 22-23 0.15285256640531486 1102 24-25 0.04529795983946627 1102 26-27 0.2208653684548736 1102 28-29 0.15784498310277684 1102 30-31 0.1490699795176127 1102 32-33 0.21688150867724687 1102 34-35 0.17652699507662817 1102 36-37 0.17144377501005437 1102 38-39 0.09040981084600475 1102 40-41 0.24335810622320508 1102 42-43 0.3776475790589231 1102 44-45 0.28795986551590147 1102 46-47 0.24968771768807585 1102 48-49 0.2642626740869005 1102 50-51 0.23098986790582643 1102 52-53 0.30167826706806267 1102 54-55 0.36863895566965965 1102 56-57 0.5105026061845592 1102 58-59 0.5116969369770104 1102 60-61 0.4139882834973321 1102 62-63 0.48702177362367394 1102 64-65 0.4457946700109474 1102 66-67 0.5098783751793832 1102 68-69 0.47547754425288247 1102 70-71 0.4337790064478142 1102 72-73 0.5115773443189866 1102 74-75 0.40748905235537336 1102 76-77 0.4882849518863779 1102 78-79 0.5806017096944132 1102 80-81 0.43780439551947126 1102 82-83 0.43696084737105423 1102 84-85 0.42478587081858166 1102 86-87 0.2840950364787389 1102 88-89 0.31105325521207305 1102 90-91 0.28937347653408096 1102 92-93 0.5177383634793742 1102 94-95 0.5909159030840598 1102 96-97 0.3355277359858846 1102 98-99 0.466456085800786 1102 100-101 0.4063056102618603 2305 1 0.18976227828693126 2305 2 0.17149300273024082 2305 3 0.07088423322973014 2305 4 0.19974672092739354 2305 5 0.20429036098931874 2305 6 0.2672159264755365 2305 7 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quality scores pass #Quality Count 2 339.0 3 165.0 4 91.0 5 135.0 6 316.0 7 2124.0 8 7274.0 9 13076.0 10 13530.0 11 12486.0 12 9044.0 13 6566.0 14 5013.0 15 4463.0 16 3958.0 17 4099.0 18 4133.0 19 4735.0 20 5381.0 21 6220.0 22 7321.0 23 7944.0 24 8876.0 25 10497.0 26 12437.0 27 14777.0 28 18120.0 29 22979.0 30 28338.0 31 36940.0 32 50741.0 33 73311.0 34 113887.0 35 193951.0 36 394400.0 37 792314.0 38 361830.0 39 1120.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.35558695354304 24.945414361494837 26.152348650529127 14.546650034432995 2 29.527751683106306 23.59444610501445 34.630446076547535 12.24735613533171 3 37.9693186386291 20.09496654203679 26.6301641058844 15.305550713449708 4 28.235353747778614 19.569981490859472 36.32050913252465 15.874155628837267 5 30.993992645325957 18.378014238008635 35.069611500682555 15.558381615982855 6 23.802384696552565 18.862668822666084 41.76219172334462 15.572754757436732 7 31.940468180456662 18.933159360364918 32.09943479524549 17.026937663932934 8 27.799792468887 28.21529908699642 27.827833610708076 16.15707483340851 9 30.37732939884747 26.831226641582173 25.608046470893264 17.183397488677095 10-11 24.2419052480689 29.202750153108383 29.91907747117597 16.636267127646743 12-13 25.00277746670151 26.058259252741472 29.94129101985022 18.997672260706803 14-15 23.24785747471419 26.8940443451797 30.9785015354459 18.8795966446602 16-17 22.441428141374487 28.079367353943873 32.01616495541641 17.463039549265236 18-19 21.885784207222063 28.261837385651585 32.228315176654945 17.624063230471407 20-21 21.692294396645856 29.064607295110907 31.638621792201555 17.604476516041686 22-23 22.21964791984644 28.797143516567637 31.21788616405276 17.765322399533158 24-25 21.615500827809957 29.289340361713617 31.172878231372714 17.92228057910371 26-27 21.174025731431108 29.852551850356974 31.055575754947647 17.91784666326427 28-29 21.200428015036948 29.506425445791614 30.91006952689766 18.383077012273777 30-31 21.490379238978647 29.191123292519734 30.850709293099115 18.467788175402504 32-33 20.442396260071874 29.23165435243009 32.26415232810348 18.061797059394554 34-35 20.143895451628097 30.263679341582232 31.713975765678853 17.878449441110813 36-37 20.375718750931313 30.374068520588516 31.30669330758203 17.943519420898145 38-39 20.58489401147957 29.406432259510858 31.77364858853965 18.23502514046993 40-41 20.979434508473567 29.64292552918228 30.686534209953358 18.691105752390797 42-43 20.918210511565213 29.166779734908726 31.36949737644607 18.54551237707999 44-45 20.30936398136833 29.590461332838146 31.690805033530555 18.409369652262967 46-47 20.87360403370227 29.71730229700642 30.890506772995924 18.51858689629539 48-49 20.433036611859485 30.070650017525725 30.629048931464638 18.86726443915015 50-51 20.325233630725002 29.491640627380537 30.822685750799973 19.360439991094484 52-53 21.173897330686817 29.180391306523447 30.345904291695163 19.29980707109458 54-55 21.53648732694583 29.28298577409728 30.04325562086258 19.137271278094307 56-57 21.731070722524777 29.29957948528643 29.95839328243867 19.010956509750116 58-59 21.43489617035773 29.206742689364074 30.46918465723743 18.889176483040764 60-61 20.400557583626426 29.481500584706914 30.32125531905433 19.796686512612325 62-63 20.867185659092222 29.443142764412713 29.997332420428325 19.692339156066744 64-65 20.31422982015428 29.509548051486117 30.393326367738922 19.78289576062068 66-67 20.151646329614543 29.09565818277867 30.460755292369303 20.29194019523748 68-69 19.83452425389518 29.31667667468271 31.301354072343617 19.547444999078493 70-71 19.482768202704534 30.81451725847362 30.38485051251898 19.31786402630286 72-73 19.523870711870796 31.19138808314022 29.791615905982617 19.493125299006365 74-75 19.85352582548158 30.705516310684228 29.899923910963157 19.541033952871032 76-77 19.947458714941632 30.587035592418882 30.06446833906269 19.401037353576793 78-79 19.856305204384604 30.65940256328858 30.160612357956946 19.32367987436987 80-81 19.401709230695012 31.18255545128808 29.99295027375214 19.422785044264767 82-83 19.249109948342536 30.83167850066601 30.027907928297747 19.891303622693705 84-85 19.353117867543332 30.371946218941847 30.404056171959915 19.8708797415549 86-87 19.55787452608208 30.42012104990568 30.61770498861085 19.40429943540139 88-89 19.848166385373872 30.93463117210471 29.875073974185707 19.342128468335712 90-91 20.04326537273748 30.5253458639411 29.64378443269286 19.78760433062856 92-93 19.621323495901578 30.82442216241055 29.91423444925551 19.640019892432363 94-95 19.223736445300005 31.141068031549768 29.954428063189354 19.680767459960876 96-97 19.493518687120325 31.475860186043015 29.603868755609714 19.42675237122694 98-99 20.068941088101347 30.780070057344915 29.2417258575554 19.909262996998336 100-101 20.03143658243252 30.636902135623856 28.931885625636028 20.399775656307597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 174.5 2 209.5 3 206.0 4 240.0 5 265.5 6 289.0 7 370.5 8 496.5 9 605.5 10 692.0 11 779.0 12 797.5 13 767.5 14 748.5 15 731.5 16 733.0 17 793.0 18 907.5 19 1060.5 20 1349.5 21 1903.5 22 2706.0 23 3850.0 24 5522.0 25 7821.5 26 11572.0 27 16706.0 28 22411.0 29 28238.5 30 34234.0 31 39838.0 32 46859.0 33 57033.0 34 68990.5 35 84910.0 36 104538.0 37 124204.5 38 144643.0 39 156775.5 40 157388.0 41 146851.0 42 129879.0 43 119204.5 44 108204.0 45 98363.0 46 95147.0 47 86354.0 48 69626.5 49 55815.5 50 45939.0 51 36932.5 52 29500.0 53 23817.5 54 19185.5 55 15091.5 56 11251.0 57 8017.5 58 5790.5 59 4203.0 60 3096.5 61 2226.0 62 1565.5 63 1105.5 64 756.0 65 560.5 66 430.0 67 319.0 68 251.0 69 196.5 70 140.5 71 102.0 72 81.0 73 69.0 74 64.0 75 49.5 76 37.5 77 40.0 78 38.0 79 32.0 80 28.0 81 27.0 82 21.5 83 16.5 84 10.5 85 9.0 86 8.5 87 6.0 88 7.0 89 9.5 90 9.0 91 5.0 92 4.5 93 4.5 94 3.0 95 2.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22579475358987913 2 0.20892783667142933 3 0.18371623454069388 4 0.16742634372734896 5 0.1784786129712805 6 0.13693273340373052 7 0.12308410688121386 8 0.11833473816996615 9 0.10670544282092972 10-11 0.09208005038769497 12-13 0.118911764275071 14-15 0.11573812069699428 16-17 0.1057955170398028 18-19 0.09960358306579295 20-21 0.10393127885407942 22-23 0.09105915804789405 24-25 0.41548098898723484 26-27 0.20033902503006082 28-29 0.09212443701116456 30-31 0.17605954199218707 32-33 0.20650876569233587 34-35 0.205598839911209 36-37 0.2112803277153184 38-39 0.21141348758572723 40-41 0.2074852714086672 42-43 0.22308716955823324 44-45 0.20455575425967326 46-47 0.22506237430263065 48-49 0.21336649901838983 50-51 0.2168508489607538 52-53 0.2083286172545897 54-55 0.19181679332389673 56-57 0.19956225911934275 58-59 0.19494605027850387 60-61 0.17492768309371215 62-63 0.16427489346100702 64-65 0.16782582333857538 66-67 0.1810974237559872 68-69 0.17313002484319315 70-71 0.16627229151713924 72-73 0.16933496853654195 74-75 0.15945894481455491 76-77 0.17055560068195608 78-79 0.16818091632633225 80-81 0.17344073120748038 82-83 0.1982306604152546 84-85 0.19561184963054795 86-87 0.2006053447708785 88-89 0.20775159114948483 90-91 0.1886653430575548 92-93 0.17020050769419925 94-95 0.1708663070462433 96-97 0.17943292537587702 98-99 0.19283768566369766 100-101 0.20395653484283363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2252931.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.52249034661725 #Duplication Level Percentage of deduplicated Percentage of total 1 93.15506365109863 64.76372013422021 2 3.457639309093273 4.807673909770427 3 1.026415532687392 2.1407689188863164 4 0.5270282280647614 1.4656125959210864 5 0.3709016673706822 1.2893003794661246 6 0.23224938176545531 0.9687933241078013 7 0.1698975430167391 0.8268190206006663 8 0.11008571619004 0.6122746512898002 9 0.117260658856381 0.7337027721042653 >10 0.7142346345787586 9.285990946496451 >50 0.06275916370148901 3.0406843825489913 >100 0.05242918169554871 7.507031789706999 >500 0.0031987068333931197 1.410549646974493 >1k 8.366250475054142E-4 1.1470775279064582 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2812 0.12481518519652844 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2742 0.12170812155365611 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2626 0.11655927323118195 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2524 0.11203183763728228 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2261 0.10035815566477622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.433458459224894E-4 0.0 0.0 2 0.0010652789632705128 0.0 0.0 3 0.0011540522102097223 0.0 0.0 4 0.0012428254571489317 0.0 4.4386623469604704E-5 5 0.0014203719510273505 0.0 4.4386623469604704E-5 6 0.001642305068375374 0.0 4.4386623469604704E-5 7 0.001953011432662607 0.0 4.4386623469604704E-5 8 0.0023524910438890495 0.0 4.4386623469604704E-5 9 0.0026188107847066777 0.0 4.4386623469604704E-5 10-11 0.0034177700071595625 0.0 4.4386623469604704E-5 12-13 0.0042833091648168545 0.0 4.4386623469604704E-5 14-15 0.0054373613750265766 0.0 4.4386623469604704E-5 16-17 0.006835540014319125 0.0 4.4386623469604704E-5 18-19 0.00812275209493766 0.0 6.657993520440705E-5 20-21 0.009587510669434616 0.0 8.877324693920941E-5 22-23 0.011496135478627618 0.0 8.877324693920941E-5 24-25 0.013693273340373052 0.0 8.877324693920941E-5 26-27 0.015823831266914078 0.0 8.877324693920941E-5 28-29 0.018797735039377594 0.0 8.877324693920941E-5 30-31 0.03036045045320962 0.0 1.1096655867401175E-4 32-33 0.03386699370730839 0.0 1.331598704088141E-4 34-35 0.03699625066191552 0.0 1.331598704088141E-4 36-37 0.040280860798666274 0.0 1.331598704088141E-4 38-39 0.044253463599195894 0.0 1.331598704088141E-4 40-41 0.048226066399725515 0.0 1.331598704088141E-4 42-43 0.053596847839547684 0.0 1.331598704088141E-4 44-45 0.05885666272069584 0.0 1.331598704088141E-4 46-47 0.06484885688909248 0.0 1.331598704088141E-4 48-49 0.07097421092789793 0.0 1.331598704088141E-4 50-51 0.07769878438354305 0.0 1.331598704088141E-4 52-53 0.08508915719123222 0.0 1.331598704088141E-4 54-55 0.09276804305147383 0.0 1.331598704088141E-4 56-57 0.10058008878212427 0.0 1.331598704088141E-4 58-59 0.11007882620461967 0.0 1.331598704088141E-4 60-61 0.12115328876028605 0.0 1.331598704088141E-4 62-63 0.13278258410932248 0.0 1.331598704088141E-4 64-65 0.14438968614662412 0.0 1.331598704088141E-4 66-67 0.15803857286352757 0.0 1.331598704088141E-4 68-69 0.17321879809013235 0.0 1.331598704088141E-4 70-71 0.18897604942184204 0.0 1.331598704088141E-4 72-73 0.20717456504437998 0.0 1.331598704088141E-4 74-75 0.2273260920995805 0.0 1.331598704088141E-4 76-77 0.250961969097145 0.0 1.331598704088141E-4 78-79 0.2762845377865545 0.0 1.331598704088141E-4 80-81 0.3056240958999632 0.0 1.331598704088141E-4 82-83 0.33873651700828833 0.0 1.331598704088141E-4 84-85 0.3762210205283695 0.0 1.331598704088141E-4 86-87 0.41816637970714593 0.0 1.331598704088141E-4 88-89 0.4662370929247278 0.0 1.331598704088141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 835 0.0 76.77512 2 GAGCGGC 655 0.0 68.82661 9 AGAGCGG 695 0.0 66.23095 8 CGGGAGA 790 0.0 64.910065 4 GAGAGCG 805 0.0 61.31129 7 AACGCGG 25 0.0050216615 57.000683 1 GATCGGG 1210 0.0 55.74447 1 GATCCGG 250 0.0 53.200638 1 TCGGGAG 985 0.0 50.61599 3 TTGTGTT 28775 0.0 48.218647 1 CGGAAAG 385 0.0 48.097137 5 TCGGGAA 415 0.0 44.62222 3 GGTTCGG 270 0.0 42.222725 1 GTTCGGA 170 0.0 41.90015 2 ATTCGGA 255 0.0 40.96904 2 AGCGGCG 605 0.0 40.787025 10-11 GAGCGTC 1835 0.0 40.083874 9 AAGCGGC 225 0.0 40.07238 9 AGAGCGT 1815 0.0 40.00266 8 GGCGCCG 365 0.0 39.679905 56-57 >>END_MODULE