FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252931
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28120.12481518519652844No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27420.12170812155365611No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26260.11655927323118195No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG25240.11203183763728228No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA22610.10035815566477622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8350.076.775122
GAGCGGC6550.068.826619
AGAGCGG6950.066.230958
CGGGAGA7900.064.9100654
GAGAGCG8050.061.311297
AACGCGG250.005021661557.0006831
GATCGGG12100.055.744471
GATCCGG2500.053.2006381
TCGGGAG9850.050.615993
TTGTGTT287750.048.2186471
CGGAAAG3850.048.0971375
TCGGGAA4150.044.622223
GGTTCGG2700.042.2227251
GTTCGGA1700.041.900152
ATTCGGA2550.040.969042
AGCGGCG6050.040.78702510-11
GAGCGTC18350.040.0838749
AAGCGGC2250.040.072389
AGAGCGT18150.040.002668
GGCGCCG3650.039.67990556-57
Produced by FastQC (version 0.11.2)