FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230062
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA322081.4442647782886753No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC225991.013379897061158No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT123120.5520922736677276No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT119460.5356801739144472No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC105730.47411237893834346No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT60690.2721449000072644No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT58280.2613380255795579No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51810.23232537929438732No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC30640.13739528318046762No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT30020.13461509141898298No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC27990.12551220549025094No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC25940.11631963595630974No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA25220.11309102616877915No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23900.10717190822497312No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT23530.10551276152860323No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA23410.10497465989734815No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC22460.10071468864991198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10950.073.2320569
AGAGCGG12100.065.8798458
CGGGAGA15000.064.885084
GTTCGGA2000.064.112522
AAGCGGC3250.062.7789089
TCGGGAG15500.060.035293
ATCGGGA19350.059.8849832
GGTCCGG1850.053.9132731
TCGGGAA5200.052.954993
TTGTGTT290300.052.2886961
TCGGAGA4000.051.0376554
CGGAGAG7400.049.4016275
ATCCGGA2700.049.2496722
GGATCGG6000.049.078191
GAACCGG1700.047.495021
GGTTCGG3250.045.3029441
AAGGGGC10650.044.553229
GATCGGG28050.044.5318761
TCGGAAA5250.044.311764
GATCCGG3250.043.841561
Produced by FastQC (version 0.11.2)